Abstract We evaluate recent and upcoming low-redshift neutral hydrogen (HI) surveys as a cosmological probe of small scale structure with a goal of determining the survey criteria necessary to test ultra-light axion (ULA) dark matter models. Standard cold dark matter (CDM) models predict a large population of low-mass galactic halos, whereas ULA models demonstrate significant suppression in this small-scale regime, with halo mass cutoffs of 1012 M⊙ to 107 M⊙ corresponding to ULA masses of 10−24 eV to 10−20 eV, respectively, if ULAs compose all of the dark matter. We generate random, homogeneously populated mock universes with cosmological parameters adjusted to match CDM and ULA models. We simulate observations of these mock universes with hypothetical analogs of the mass-limited ALFALFA and WALLABY HI surveys and reconstruct the corresponding HI mass function (HIMF). We find that the ALFALFA HIMF can test for the presence of ULA DM with ma ≲ 10−21.5 eV, while WALLABY could reach the larger window ma ≲ 10−20.9 eV. These constraints are complementary to other probes of ULA dark matter, demonstrating the utility of local Universe HI surveys in testing dark matter models.