Low-level Sustained Contractions Research Articles

Medial gastrocnemius is a key muscle for involuntary alternate muscle activity of plantar flexor synergists

Redundant and/or complicated muscle activations between synergist muscles have been demonstrated during low-level sustained contractions. Identification of a key muscle for this phenomenon allows for the simplification of motor control during prolonged contraction. In this study, we have identified a key muscle for involuntary alternate muscle activity of plantar flexor muscles based on a physiological tremor sequence that was recorded over 120min. Two epochs where the muscle activity of medial gastrocnemius abruptly increased with decrease in other synergists (case ON) and vise verse (case OFF) were analyzed. Our results indicated that involuntary alternate muscle activity was associated with changes in physiological tremor of ankle angular acceleration when the muscle activity of medial gastrocnemius decreased in case OFF. In particular, the activity of the medial gastrocnemius muscle, but not the activity of other synergists, was accompanied by physiological tremor, demonstrating that the medial gastrocnemius is a key muscle for involuntary alternate muscle activity in plantar flexor synergists. In addition, weaker correlations between muscle activities and physiological tremor were found in case ON than case OFF. We suggest that, if the central nervous system can employ this unique muscle strategy, redundant and/or complicated neuromuscular activities will be reduced because of the existence of the key muscle.

Alternate muscle activity patterns among synergists of the quadriceps femoris including the vastus intermedius during low‐level sustained contraction in men

We quantified the alternate muscle activity among four synergists of the quadriceps femoris (QF), including the vastus intermedius (VI), during low-level sustained contraction. Surface electromyograms (EMGs) were recorded from the VI, vastus lateralis (VL), vastus medialis (VM), and rectus femoris (RF) in 11 healthy men during isometric knee extension at 2.5% maximum voluntary contraction for 60 min to determine alternate muscle activity among the four synergists of the QF. Alternate activity was primarily found between the RF and VI, VL, or VM, and rarely found among the vasti muscles. Multiple muscle comparison revealed the duration of alternate activity in the RF/VI+VL+VM combination remained high throughout the experiment, although the frequency of that combination did not. These results suggested that there is a fixed muscle combination, i.e., RF and the 3 vasti muscles, to perform low-intensity sustained contraction in the human QF.

Firing duration of masseter motor units during prolonged low-level contractions

Objective The study aimed to determine whether motor units (MUs) of the masseter muscle can be continuously active during a prolonged low-level sustained contraction. Methods Intramuscular fine-wire EMG activity was recorded unilaterally from the masseter muscles of 13 pain-free volunteers (mean age ± SD = 26.7 ± 7.7 years), during low-level biting tasks performed for 30 min. The stored intramuscular EMG signals were decomposed into individual MU action-potential trains using long-term decomposition software. Depending on relative duration of the MU activity, MUs were classified as sporadically (<50% of the time), intermittently (50% ⩽ time ⩽ 95%), or continuously active (>95% of the time). Results The overall number of MUs identified during the low-level biting task was 203. Of these, nine continuously active MUs (4.4%) were found in six of the 13 subjects investigated, whereas 50 intermittent MUs (24.6%) were found in 12 subjects. The remaining MUs (71.0%) were sporadically active and were found in all of the subjects investigated. Conclusion The majority of masseter MUs is sporadically or intermittently active during prolonged low-level contractions, but some of them can also be continuously active. Significance Sustained clenching efforts may be accompanied by continuous activity of a few selected muscle fibres.

Alternate Muscle Activity Of Four Synergists In Quadriceps Femoris During Low-level Sustained Contraction

Alternate Muscle Activity Of Four Synergists In Quadriceps Femoris During Low-level Sustained Contraction

Motor Unit Rotation in a Variety of Human Muscles

The phenomena of substitution and rotation among motor units of a muscle were examined in seven different muscles. Intramuscular motor unit activity and surface electromyographic (EMG) activity were recorded from one of the following muscles: abductor digiti minimi, first dorsal interosseous, extensor digitorum communis, flexor and extensor carpi radialis, tibialis anterior, and soleus. The subject was asked to discharge a discernible unit at a comfortable constant or rhythmically (pseudosinusoidally) modulated rate with audio and visual feedback. Results are reported from a total of 42 sets of motor units from all seven muscles. We observed that when a subject fired a motor unit for a long period, an additional motor unit frequently started to discharge after a few minutes. When the subject was asked to keep activity down to one unit, very often it was Unit 1 that dropped and Unit 2 continued to fire. Whereas Unit 2 had fired for a few minutes, Unit 1 resumed firing without any conscious effort by the subject. If the subject was then asked to retain just one unit, it was Unit 2 that dropped. Rhythmic modulation of firing rate of a tonically firing unit showed that whereas the threshold of this unit increased, the threshold of a phasically discharging unit decreased substantially. The increase in threshold of a tonically discharging unit is suggested to arise from inactivation of Na(+) and Ca(2+) channels and the decrease in threshold of higher-threshold units is suggested to arise from an increase in persistent inward currents that may occur during prolonged contractions. Whether a unit stops or starts to fire is suggested to depend on a balance between the strength of the central motor command, persistent inward currents, and inactivation of voltage-gated channels. Such rotations among low-threshold motoneurons would ensure low-level sustained contractions to be viable not only in small hand muscles but also in larger limb muscles.

Finger flexor contractile properties and hemodynamics following a sustained submaximal contraction: A study using electrical stimulation and near-infrared spectroscopy

We examined the effect of a low-level sustained contraction on the muscle contractile properties, hemodynamics and oxygenation of the flexor digitorum superficialis muscle (FDS) of the finger. We tested the hypothesis that hemodynamics and oxygenation, reflecting the muscle metabolic characteristics, would recover more quickly than the muscle contractile properties. Eleven subjects (26 ± 4 years) were equipped with electrodes for electrical stimulation and a near-infrared spectroscopy (NIRS) probe on the forearm over the FDS. The experimental protocol consisted of three baselines measurements (−60, −30 min and pre-exercise), immediately after a sustained 15-min contraction of the FDS at 10% maximal voluntary contraction (post-exercise), and after 30, 60 and 120 min of recovery. For each time point, participants were subjected to a battery of test that included upper arm venous occlusion (at rest), a computer-mouse point and click task (standardized voluntary task), and electrical stimulation. For venous occlusion (50 mmHg, 1 min), slopes were calculated for NIRS-derived total hemoglobin (HbTslope) and deoxyhemoglobin (HHbslope) as estimates of blood flow and oxygen consumption, respectively. The computer-mouse task entailed using the mouse to point and click on targets presented on the screen during which NIRS signals were monitored for determination of change in total hemoglobin (ΔHbT) and oxygen saturation (ΔStO 2%). Electrical stimulation (2 Hz, 5 trains of 15 twitches) provided twitch force (Tw-force), contraction time (CT) and one-half relaxation time (½RT) data. Statistical analysis revealed significant changes over time for all contractile parameters as well as for HHbslope ( P < 0.05 for each). Post-hoc testing demonstrated significant decreases for Tw-force post-exercise and at 60 min; for CT at post-exercise, 30 and 60 min; and for ½RT at post-exercise and at 30 min. HHbslope was significantly higher post-exercise as compared to pre-exercise. During the computer-mouse point and click task, no significant differences were detected for ΔHbT, however, ΔStO 2% showed a tendency to decrease, albeit not significant ( P = 0.11). Further testing showed ΔStO 2% was significantly lower post-exercise and at 30 min as compared to pre-exercise. The present study shows that NIRS provides insight into muscle hemodynamics and oxygenation for low-level sustained activity to fatigue. The overall quick recovery of hemodynamic and oxygenation responses, and a more prolonged recovery of contractile responses confirms our hypothesis, and this may fit well with the classical definition of low-frequency fatigue. Relevance to industry Understanding changes in tissue hemodynamics, oxygenation and muscle contractile properties in response to sustained contractions may provide insight into mechanisms behind work-related musculoskeletal disorders. Detecting changes in the underlying physiology of the muscle before the onset and development of an injury, may lead to primary prevention methods to reduce the occurrence of such injuries.

The frequency of alternate muscle activity is associated with the attenuation in muscle fatigue

Alternate muscle activity between synergist muscles has been demonstrated during low-level sustained contractions [< or =5% of maximal voluntary contraction (MVC) force]. To determine the functional significance of the alternate muscle activity, the association between the frequency of alternate muscle activity during a low-level sustained knee extension and the reduction in knee extension MVC force was studied. Forty-one healthy subjects performed a sustained knee extension at 2.5% MVC force for 1 h. Before and after the sustained knee extension, MVC force was measured. The surface electromyogram was recorded from the rectus femoris (RF), vastus lateralis (VL), and vastus medialis (VM) muscles. The frequency of alternate muscle activity for RF-VL, RF-VM, and VL-VM pairs was determined during the sustained contraction. The frequency of alternate muscle activity ranged from 4 to 11 times/h for RF-VL (7.0 +/- 2.0 times/h) and RF-VM (7.0 +/- 1.9 times/h) pairs, but it was only 0 to 2 times/h for the VL-VM pair (0.5 +/- 0.7 times/h). MVC force after the sustained contraction decreased by 14% (P < 0.01) from 573.6 +/- 145.2 N to 483.3 +/- 130.5 N. The amount of reduction in MVC force was negatively correlated with the frequency of alternate muscle activity for the RF-VL and RF-VM pairs (P < 0.001 and r = 0.65 for both) but not for the VL-VM pair. The results demonstrate that subjects with more frequent alternate muscle activity experience less muscle fatigue. We conclude that the alternate muscle activity between synergist muscles attenuates muscle fatigue.

Force fluctuations are modulated by alternate muscle activity of knee extensor synergists during low-level sustained contraction.

The study examined the hypothesis that altered synergistic activation of the knee extensors leads to cyclic modulation of the force fluctuations. To test this hypothesis, the force fluctuations were investigated during sustained knee extension at 2.5% of maximal voluntary contraction force for 60 min in 11 men. Surface electromyograms (EMG) were recorded from the rectus femoris (RF), vastus lateralis (VL), and vastus medialis (VM) muscles. The SD of force and average EMG (AEMG) of each muscle were calculated for 30-s periods during alternate muscle activity. Power spectrum of force was calculated for the low- (< or =3 Hz), middle- (4-6 Hz), and high-frequency (8-12 Hz) components. Alternate muscle activity was observed between RF and the set of VL and VM muscles. The SD of force was not constant but variable due to the alternate muscle activity. The SD was significantly greater during high RF activity compared with high VL and VM activity (P < 0.05), and the correlation coefficient between the SD and AEMG was significantly greater in RF [0.736 (SD 0.095), P < 0.05] compared with VL and VM. Large changes were found in the high-frequency component. During high RF activity, the correlation coefficient between the SD and high-frequency component [0.832 (SD 0.087)] was significantly (P < 0.05) greater compared with other frequency components. It is suggested that modulations in knee extension force fluctuations are caused by the unique muscle activity in RF during the alternate muscle activity, which augments the high-frequency component of the fluctuations.

Alternate muscle activity observed between knee extensor synergists during low-level sustained contractions.

To determine quantitatively the features of alternate muscle activity between knee extensor synergists during low-level prolonged contraction, a surface electromyogram (EMG) was recorded from the rectus femoris (RF), vastus lateralis (VL), and vastus medialis (VM) in 11 subjects during isometric knee extension exercise at 2.5% of maximal voluntary contraction (MVC) for 60 min (experiment 1). Furthermore, to examine the relation between alternate muscle activity and contraction levels, six of the subjects also performed sustained knee extension at 5.0, 7.5, and 10.0% of MVC (experiment 2). Alternate muscle activity among the three muscles was assessed by quantitative analysis on the basis of the rate of integrated EMG sequences. In experiment 1, the number of alternations was significantly higher between RF and either VL or VM than between VL and VM. Moreover, the frequency of alternate muscle activity increased with time. In experiment 2, alternating muscle activity was found during contractions at 2.5 and 5.0% of MVC, although not at 7.5 and 10.0% of MVC, and the number of alternations was higher at 2.5 than at 5.0% of MVC. Thus the findings of the present study demonstrated that alternate muscle activity in the quadriceps muscle 1) appears only between biarticular RF muscle and monoarticular vasti muscles (VL and VM), and its frequency of alternations progressively increases with time, and 2) emerges under sustained contraction with force production levels < or =5.0% of MVC.

Muscle blood flow during isometric activity and its relation to muscle fatigue.

The effect of isometric exercise on blood flow, blood pressure, intramuscular pressure as well as lactate and potassium efflux from exercising muscle was examined. The contractions performed were continuous or intermittent (5 s on, 5 s off) and varied between 5% and 50% maximal voluntary contraction (MVC). A knee-extensor and a hand-grip protocol were used. Evidence is presented that blood flow through the muscle is sufficient during low-level sustained contractions (less than 10% MVC). Despite this muscle fatigue occurs during prolonged contractions. One mechanism for this fatigue may be the disturbance of the potassium homeostasis. Such changes may also play a role in the development of fatigue during intermittent isometric contractions and even more so in the recovery from such exercise. In addition the role of impaired transport of substances within the muscle, due to long-lasting daily oedema formation, is discussed in relation to fatigue in highly repetitive, monotonous jobs.