Recent anthropogenic activities have escalated human exploitation of riparian zones of river ecosystems, consequently diminishing aquatic biodiversity. This intensification of land use is also causing water quality degradation and changes in water environmental factors, evidenced by increased nutrient levels and adversely impacting the community structure and diversity of aquatic organisms. Notably, the Weihe River Basin, the largest tributary of the Yellow River, has demonstrated signs of significant anthropogenic pressure. Despite this, comprehensive investigations examining the effects of land-use intensity on aquatic organism diversity in this watershed remain limited. In this study, the environmental impacts and macroinvertebrate diversity under high-intensity and low-intensity land-use scenarios within the Weihe River Basin were investigated through field surveys conducted during the spring and autumn seasons. Our results demonstrated that areas under high-intensity land use exhibited elevated nutrient concentrations (e.g., total nitrogen) compared to those under low-intensity land use. These environmental changes significantly influenced the macroinvertebrate community structure, reducing functional and phylogenetic diversities in high-intensity land-use watersheds. Hydrological factors (water depth, river width, and discharge) have a significant impact on macroinvertebrate taxonomic diversity. Thus, understanding the effects of land-use intensity on aquatic biodiversity is essential for ecological assessments of impacted watersheds and developing management strategies for the sustainable use and planning of riparian lands in the Weihe River Basin.