Low Frequency Of Isolation Research Articles

Bacterial pathogens and antimicrobial resistance in acute otitis media

IntroductionRecent studies show an increase in the prevalence of Haemophilus influenzae and a decrease in Streptococcus pneumoniae among the bacteria that cause acute otitis media (AOM). The objective of our study was to analyse the distribution of pathogens identified in children aged less than 14 years presenting to the emergency department with AOM and their patterns of antimicrobial resistance. Patients and methodsSingle centre retrospective, analytical study in patients aged less than 14 years with a diagnosis of AOM in whom an ear drainage sample was collected for culture in the paediatric emergency department of a tertiary care hospital between 2013 and 2021. ResultsDuring the study period, there were 14 684 documented care episodes corresponding to children with a diagnosis of AOM. An ear drainage culture was performed in 768 of those episodes. The median age of the patients was 2 years, 57% were male and 70% had a previous history of AOM. The most frequently isolated pathogens were: Haemophilus influenzae (n = 188 [24.5%]; 15.5% of them resistant to ampicillin), Streptococcus pyogenes (n = 86 [11.2%]), Staphylococcus aureus (n = 82 [10.7%]), Streptococcus pneumoniae (n = 54 [6.9%]; 9.4% with intermediate resistance to penicillin), Pseudomonas aeruginosa (n = 42 [5.5%]) and Moraxella catarrhalis (n = 11 [1.4%]). No pathogen was isolated in 34.9% of cases. ConclusionsHaemophilus influenzae is the leading cause of AOM in children aged less than 14 years. This, combined with the low frequency of isolation and penicillin resistance of Streptococcus pneumoniae, calls into question the appropriateness of high-dose amoxicillin for empiric treatment of AOM.

Patógenos bacterianos y resistencia a los antibióticos en otitis media aguda

IntroductionRecent studies show an increase in the prevalence of Haemophilus influenzae and a decrease in Streptococcus pneumoniae among the bacteria that cause acute otitis media (AOM). The objective of our study was to analyse the distribution of pathogens identified in children aged less than 14 years presenting to the emergency department with AOM and their patterns of antimicrobial resistance. Patients and methodsSingle centre retrospective, analytical study in patients aged less than 14 years with a diagnosis of AOM in whom an ear drainage sample was collected for culture in the paediatric emergency department of a tertiary care hospital between 2013 and 2021. ResultsDuring the study period, there were 14,684 documented care episodes corresponding to children with a diagnosis of AOM. An ear drainage culture was performed in 768 of those episodes. The median age of the patients was 2 years, 57% were male and 70% had a previous history of AOM. The most frequently isolated pathogens were: Haemophilus influenzae (n=188 [24.5%]; 15.5% of them resistant to ampicillin), Streptococcus pyogenes (n=86 [11.2%]), Staphylococcus aureus (n=82 [10.7%]), Streptococcus pneumoniae (n=54 [6.9%]; 9.4% with intermediate resistance to penicillin), Pseudomonas aeruginosa (n=42 [5.5%]) and Moraxella catarrhalis (n=11 [1.4%]). No pathogen was isolated in 34.9% of cases. ConclusionsHaemophilus influenzae is the leading cause of AOM in children aged less than 14 years. This, combined with the low frequency of isolation and penicillin resistance of Streptococcus pneumoniae, calls into question the appropriateness of high-dose amoxicillin for empiric treatment of AOM

An Improved Structural Analysis Method for Isolator with Quasi-Zero-Stiffness Characteristic

A typical quasi-zero-stiffness (QZS) vibration isolator consisting of a vertical spring and two oblique springs has been widely researched on its static and dynamic characteristics. A general criterion for determining structural parameters of QZS isolator is to achieve low nondimensional stiffness around the equilibrium position. However, lower nondimensional stiffness of linear isolator means lower isolation frequency, which may be invalid on QZS isolator. Because there is an implicit relationship between geometric parameter and stiffness ratio of QZS isolator, this study presents an improved optimization criterion for determining the optimal structural parameters of the typical QZS isolator. The optimization criterion is that the QZS isolator has the maximum displacement range around the equilibrium position without exceeding given natural frequency, rather than given nondimensional stiffness. The results show that isolator with these optimal parameters can achieve lower stiffness around the equilibrium position and better vibration isolation performance. Furthermore, an extended QZS isolator consisting of vertical spring with fixed stiffness and prestressed oblique springs is discussed to further improve stiffness characteristic. Better stiffness performance can be obtained when the prestressed oblique springs have softening stiffness and the exponent of the nonlinear stiffness is 2. Considering the existence of friction in practical application, the influence of friction on both static and dynamic characteristics is investigated. The analysis reveals that friction has little influence on its stiffness characteristic around the static equilibrium position and friction damping produced by friction affects the response amplitude and resonant frequency in dynamics.

Low Viability of Cholera Toxin-Producing Vibrio cholerae O1 in the Artificial Low Ionic Strength Aquatic Solution.

It has been well known that Vibrio cholerae inhabit in environmental water. As many patients infected with cholera toxin-producing V. cholerae O1 (toxigenic V. cholerae O1) emerge in Kolkata, India, it has been thought that toxigenic V. cholerae O1 is easily detected in environmental water in Kolkata. However, we could not isolate toxigenic V. cholerae O1 from environmental water in Kolkata, though NAG Vibrio (generic name of V. cholerae non-O1/non-O139) is constantly detected. To clear the reason for the non-isolation of toxigenic V. cholerae O1, we examined the viability of V. cholera O1 and NAG Vibrios in the artificial low ionic strength aquatic solution. We found that the viability of toxigenic V. cholerae O1 in the solution is low, but that of NAG Vibrios is high. Subsequently, we examined the viability of NAG Vibrios possessing cholera toxin gene (ctx) in the same condition and found that the viability of these NAG Vibrios is low. These results indicate that the existence of ctx in V. cholerae affects the viability of V. cholerae in the aquatic solution used in this experiment. We thought that there was closely relation between the low viability of toxigenic V. cholerae O1 in the artificial low ionic strength aquatic solution and the low frequency of isolation of the strain from environmental water.

Improvement of vibration isolation performance of QZS platform in chaotic interval based on damping increase control method

In order to further extend the effective isolation range of the quasi-zero stiffness (QZS) platform, the vibration isolation performance of the chaotic interval is improved. For a class of self-design quasi-zero-stiffness (QZS) vibration isolation platform, starting with chaotic motion of the non-linear vibration isolation system, the jumping interval is eliminated by means of transient chaos phenomena. The control method of damping increase is proposed in combination with the application of the Von der Pol Plane, which can make the jumping-down frequency to be decreased below the external excitation frequency, thus the effective isolation range of vibration isolation platform can be extended, and a lower isolation frequency can be obtained. Research shows that, when the solution of the motion equation of the platform system falls to resonant branch led by certain initial conditions, the resonant amplitude response will jump to the non-resonant branch by transient damping increase control method, which the removal time is determined by the Von der Pol Plane, hence making the platform system obtain the ideal vibration isolation performance. The research results have a significant importance for improving the low frequency and ultra-low frequency vibration isolation effect of such platforms, which lays a foundation for the popularization and engineering application, and can also provide a reference for control of other nonlinear vibration system.

Investigation on a two-stage platform of large stroke for broadband vertical vibration isolation

For the purpose of preventing vibration-sensitive optical switches from malfunction caused by broadband vertical vibration, a novel two-stage vibration isolation platform is proposed. The primary stage is a bellows-type isolator of large stroke and low isolation frequency, and the secondary stage is a small-stroke hybrid isolator composed of bellows and voice-coil actuators. In the primary stage, two pre-compressed horizontal bellows and one vertical bellows are used to counter the weight of the switch and to reduce the total height of the isolator. The static properties of the primary stage are analyzed, and the vibration isolation of the platform is investigated. Numerical results indicate that the two-stage platform is effective in isolating vertical vibration. Experiments are also conducted to verify the performance of the platform. It is exhibited that the transmissibility is less than 0 dB over 2 Hz, and the attenuation rate reaches −35 dB/dec at high frequencies. The frequency range of test is 2–200 Hz, and the maximum displacement is 10 mm at 2 Hz. In the secondary stage, the actuators can substantially suppress the resonance peak, and promote isolation performance at low frequencies.

Pilidium lythri Is Associated with Bunch Rot of Grapevine (Vitis vinifera)

Bunch rot of Vitis vinifera is frequently caused by a complex of filamentous fungi. In a study on non-Botrytis fungi associated with bunch rot at harvest in northwestern Spain, rotting berries showing pink masses were observed in bunches of V. vinifera Albarino in one vineyard that experienced prolonged warm, moist conditions before harvest. The aim of this work was to identify the fungal species and determine its pathogenicity on grapes. Fungal isolates not corresponding morphologically to any known genus associated with bunch rot were obtained from the pink masses. Morphological and molecular phylogenetic analyses revealed that the isolates belonged to Pilidium lythri, an opportunistic pathogen causing tan-brown rot on strawberry. Most frequent non-Botrytis grape-rotting fungi at the vineyard were Penicillium brevicompactum, Penicillium expansum, and Talaromyces purpurogenus, while P. lythri had a low isolation frequency. Pathogenicity tests showed that P. lythri caused tan-brown rot in berries of the V. vinifera table grape varieties Regal Seedless and Red Globe. That P. lythri can directly infect healthy grape berries suggests that it may be a true pathogen associated with bunch rot on grapevine. Its presence on berries may contribute to postharvest decay of table and wine grapes.

Designing Metamaterial with Arc-Structure for Wide Broad Vibration Isolating

The paper is devoted to the idea of metamaterials with quasi-zero stiffness for vibration isolation. Metametarials may provide special and unique properties of materials. In this area it is possible to use the principles of systems with quasi-zero stiffness. Such nonlinear deformation of material provides low stiffness at a certain point. Hence low natural frequency and high efficiency of vibration isolation can be obtained. An analytical study was done. Computer modeling shows a big enough margin of safety and also proves the existence of force characteristic with quasi-zero stiffness. The calculation has shown that the metamaterial has natural frequency lower than 1 Hz and optimum load 2 tons per m2. An experimental prototype has been made for further study.

H∞ active control of frequency-varying disturbances in a main engine on the floating raft vibration isolation system

Reducing the vibration of marine power machinery can improve warships' capabilities of concealment and reconnaissance. Being one of the most effective means to reduce mechanical vibrations, the active vibration control technology can overcome the poor effect in low frequency of traditional passive vibration isolation. As the vibrations arising from operation of marine power machinery are actually the frequency-varying disturbances, the H∞ control method is adopted to suppress frequency-varying disturbances. The H∞ control method can solve the stability problems caused by the uncertainty of the model and reshape the frequency response function of the closed loop system. Two-input two-output continuous transfer function models were identified by using the system identification method and are validated in frequency domain of which all values of best fit exceeds 89%. The method of selecting the weighting functions on the mixed sensitivity problem is studied. Besides, the H∞ controller is designed for a multiple input multiple output (MIMO) system to suppress the single-frequency-varying disturbance. The numerical simulation results show that the magnitudes of the error signals are reduced by more than 50%, and the amplitudes of the dominant frequencies are attenuated by more than 10 dB. Finally, the single excitation source dual-channel control experiments are conducted on the floating raft isolation system. The experiment results reveal that the root mean square values of the error signals under control have fallen by more 74% than that without control, and the amplitudes of the error signals in the dominant frequencies are attenuated above 13 dB. The experiment results and the numerical simulation results are basically in line, indicating a good vibration isolation effect.

Phage Biodiversity in Artisanal Cheese Wheys Reflects the Complexity of the Fermentation Process.

Dairy fermentations constitute a perfect “breeding ground” for bacteriophages infecting starter cultures, particularly strains of Lactococcus lactis. In modern fermentations, these phages typically belong to one of three groups, i.e., the 936, P335, and c2 phage groups. Traditional production methods present fewer chemical and physical barriers to phage proliferation compared to modern production systems, while the starter cultures used are typically complex, variable, and undefined. In the current study, a variety of cheese whey, animal-derived rennet, and vat swab samples from artisanal cheeses produced in Sicily were analysed for the presence of lactococcal phages to assess phage diversity in such environments. The complete genomes of 18 representative phage isolates were sequenced, allowing the identification of 10 lactococcal 949 group phages, six P087 group phages, and two members of the 936 group phages. The genetic diversity of these isolates was examined using phylogenetic analysis as well as a focused analysis of the receptor binding proteins, which dictate specific interactions with the host-encoded receptor. Thermal treatments at 63 °C and 83 °C indicate that the 949 phages are particularly sensitive to thermal treatments, followed by the P087 and 936 isolates, which were shown to be much less sensitive to such treatments. This difference may explain the relatively low frequency of isolation of the so-called “rare” 949 and P087 group phages in modern fermentations.

Diagnostic value of sepsis biomarkers in hematopoietic stem cell transplant recipients in a condition of high prevalence of gram-negative pathogens

Objective/backgroundA decision about the need for antimicrobial therapy in a patient with febrile neutropenia after hematopoietic stem cell transplantation (HSCT) is often complicated because of the low frequency of culture isolation and reduced clinical manifestation of infection. Usefulness and choice of sepsis biomarkers to distinguish bloodstream infection (BSI) from other causes of febrile episode is still argued in HSCT recipients in modern epidemiological situations characterized by the emergence of highly resistant gram-negative microorganisms. In this study a comparative analysis of diagnostic values of presepsin, procalcitonin (PCT), and C-reactive protein (CRP) was performed as sepsis biomarkers in adult patients after HSCT in a condition of high prevalence of gram-negative pathogens. MethodsA prospective observational clinical study was performed at the Center of Hematology and Bone Marrow Transplantation in Minsk, Republic of Belarus. The biomarkers (presepsin, PCT, and CRP) were assessed in a 4-hour period after the onset of febrile neutropenia episode in adult patients after HSCT. Microbiologically-confirmed BSI caused by a gram-negative pathogen was set as a primary outcome. ResultsClinical and laboratory data were analyzed in 52 neutropenic patients after HSCT aged 18–79years. Out of the biomarkers assessed, the best diagnostic value was shown in presepsin (area under the curve [AUC]: 0.889, 95% confidence interval [CI]: 0.644–0.987, p<.0001) with 75% sensitivity and 100% specificity, then in PCT (AUC: 0.741, 95% CI: 0.573–0.869, p=.0037) with 62% sensitivity and 88% specificity. The optimal cut-off value for CRP was set as 165mg/L, while it had an average diagnostic value (AUC: 0.707, 95% CI: 0.564–0.825, p=.0049) with low sensitivity (40%) and should not be routinely recommended as a biomarker in adult patients with suspected BSI after HSCT. ConclusionPresepsin may be recommended in adult patients with suspected gram-negative BSI after HSCT as a possible additional supplementary test with a cut-off value of 218pg/mL. PCT is inferior to presepsin in terms of sensitivity and specificity, but still shows a good quality of diagnostic value with an optimal cut-off value of 1.5ng/mL. CRP showed an average diagnostic value with low sensitivity (40%) and should not be routinely recommended as a biomarker in adult patients with suspected BSI after HSCT in a condition of high prevalence of gram-negative pathogens.

Salmonella spp. Isolated by Miniaturized Most Probable Number and Conventional Microbiology in Poultry Slaughterhouses

Background: Salmonella spp. are frequently isolated from fowls, and their detection in poultry products varies according to the breeding system and the slaughtering process, bringing risks to the consumer and compromising the marketability. The control of Salmonella in poultry slaughterhouses is based on the detection of bacteria, but the quantification of the agent would be important in assessing risk, as well as in obtaining data to determine the capacity of each step of the process to decrease or increase bacterial contamination. The aims of this study were to propose a method for the quantification of Salmonella in poultry slaughterhouses, frequency of isolation and serovars identified.Materials, Methods &amp; Results: Twenty-one broiler flocks from seven federally inspected slaughterhouses in southern Brazil, totaling 1,071 samples, were assessed by miniaturized most probable number (mMPN) and conventional microbiology. The samples were collected in triplicate at 17 points, which included cloacae, transportation cages before and after sanitization, water (scald tank, supply, pre-chiller and chiller), and carcasses (before and after scalding, defeathering, rinsing, evisceration, final rinsing, chilling at 4ºC, and freezing at -12°C for 24 h, 30 and 60 days). Typical Salmonella colonies were submitted to TSI, LIA, SIM, urea, and polyvalent anti-O antiserum tests, and to final identification by Microarray by Check&amp;Trace. Nine of the 1,071 (0.83%) samples analyzed by mMPN and by conventional microbiology were positive for Salmonella and the following serovars were identified: Anatum, Brandenburg, Agona, Tennessee, Bredeney, Schwarzengrund and Infantis.Discussion: This positive rate was lower than that described by other authors, whose rates ranged from 3% and 39% for the isolation of Salmonella spp. from different sources, such as slaughterhouses and retail sales in samples collected in Brazil. The low frequency of isolation of Salmonella in this study can be attributed to the efficiency of control systems used from the field to the slaughterhouse, such as Good Manufacturing Practices (GMP) and Sanitation Standard Operating Procedures (SSOP), which are HACCP requirements. Also, when slaughtering technology actions are properly managed, such as water replacement and temperatures lower than 4ºC in the chiller, the initial contamination by Salmonella spp. can be reduced, with a decline in contamination from 70% to 20%, and with a reduction in the contamination of broiler carcasses after chilling from 15.8% to 3.3%. On the other hand the contamination of carcasses by Salmonella before pre-chilling and in post-chilling might be due to the automated system, inadequate temperatures during chilling, and inappropriate water chlorination in the assessed meat-packing plant. Of the 17 points evaluated, seven were positive for Salmonella, especially the cages after sanitization and frozen carcasses. The contamination by Salmonella spp. in transportation cages after sanitization indicates inefficiency of the automated system as well as possible bacterial resistance to the sanitizers used in SSOP while the isolation in carcasses frozen for 24 h and 60 days demonstrates the thermal resistance of the bacterium to a conservation method widely used in the food industry. In this work, just one of the nine positive samples for Salmonella was identified by conventional methods (CM) and mMPN. The discrepancy between methods can be explained by the heterogeneous distribution of Salmonella and other bacteria in naturally contaminated samples. Samples that were positive in the qualitative test but negative in the mMPN protocol could have had a number of Salmonella below the detection amount.

Farmers acquire antimicrobial resistant Staphylococcus aureus from pigs

Carriage of animal-associated MRSA CC398 is common among pig farmers. This study was conducted to investigate: 1) whether pig farmers are colonized with pig-specific S. aureus genotypes different than CC398, and 2) survey antimicrobial resistance of S. aureus isolates from pigs and pig farmers. Forty-eight S. aureus isolates from pig farmers and veterinarians and 130 isolates from pigs collected in Western Switzerland were genotyped by spa-typing and amplified fragment length polymorphism (AFLP). Antimicrobial resistance profiles were determined for representative sample of the isolates. Obtained earlier data on healthy S. aureus carriers without exposure to agriculture were used for comparison. The genotype composition of S. aureus isolates from pig farmers and veterinarians was similar to isolates from pigs with predominant AFLP Clusters CC398, CC9 and CC49. The resistance to tetracycline and macrolides was common among the isolates from farmers and veterinarians: 52% isolates were resistant to tetracycline and 21% to clarithromycin. The resistance levels among isolates from pigs were very similar: 39% resistant to tetracycline and 23% resistant to clarithromycin. This was in contrast to isolates from persons without contact with agriculture, where no (0/128) isolates were resistant to tetracycline and 3% of isolates were resistant to clarithromycin. MRSA CC398 was isolated from pigs (n=11) and pig farmers (n=5). These data imply that zoonotic transmission of multidrug resistant S. aureus from pigs to farmers is very frequent, and well-known MRSA transmission merely represents a tip of an iceberg of this phenomenon. We speculate that relatively low frequency of MRSA isolation is related to lower antimicrobial use in Switzerland compared to e.g. the Netherlands.

Fungal communities of symptomless bark of tropical trees

The fungi associated with the bark (phellophytes) of fifteen dicotyledonous tree species of a dry thorn, dry deciduous and a stunted montane evergreen forest of the Western Ghats, southern India, were studied. The species diversity of the phellophytes was higher for the montane evergreen forest when compared with dry thorn and dry deciduous forests. Although many fungal species were present in the bark of different tree species, most of them had a low frequency of isolation and only a few were isolated with high frequencies. Species of Phoma and Phomopsis, Fusarium and Paecilomyces and sterile forms EGS1 and EGS3 were isolated with high frequency from dry thorn, dry deciduous and a stunted montane evergreen forest respectively. The dry thorn and dry deciduous forests, which are more arid and fire-prone, shared more phellophyte species than the dry deciduous and montane evergreen forests. The phellophyte assemblages of the different forests were distinct while those of six tree species of a family from one forest showed a high overlap. It appears that the environment, rather than the tree species, determines the phellophyte assemblage in these forests.

Antimicrobial Resistance of Staphylococcus aureus Strains Acquired by Pig Farmers from Pigs

Carriage of animal-associated methicillin-resistant Staphylococcus aureus (MRSA) clonal complex 398 (CC398) is common among pig farmers. This study was conducted (i) to investigate whether pig farmers are colonized with pig-specific S. aureus genotypes other than CC398 and (ii) to survey antimicrobial resistance of S. aureus isolates from pigs and pig farmers. Forty-eight S. aureus isolates from pig farmers and veterinarians and 130 isolates from pigs collected in Western Switzerland were genotyped by spa typing and amplified fragment length polymorphism (AFLP). Antimicrobial resistance profiles were determined for representative sample of the isolates. The data obtained earlier on healthy S. aureus carriers without exposure to agriculture were used for comparison. The genotype composition of S. aureus isolates from pig farmers and veterinarians was similar to isolates from pigs with predominant AFLP clusters CC398, CC9, and CC49. The resistance to tetracycline and macrolides (clarithromycin) was common among the isolates from farmers and veterinarians (52 and 21%, respectively) and similar to resistance levels in isolates from pigs (39 and 23%, respectively). This was in contrast to isolates from persons without contact with agriculture, where no (0/128) isolates were resistant to tetracycline and 3% of the isolates were resistant to clarithromycin. MRSA CC398 was isolated from pigs (n = 11) and pig farmers (n = 5). These data imply that zoonotic transmission of multidrug-resistant S. aureus from pigs to farmers is frequent, and well-known MRSA transmission merely represents the tip of the iceberg for this phenomenon. We speculate that the relatively low frequency of MRSA isolation is related to lower antimicrobial use in Switzerland compared to, for example, the Netherlands.

Yeasts from phylloplane and their capability to produce indole-3-acetic acid

Yeasts were isolated from the phylloplane of various plant species collected from seven provinces in Thailand. A total of 114 yeast strains and 10 strains of a yeast-like fungus were obtained by enrichment isolation from 91 out of 97 leaf samples (93.8 %). On the basis of the D1/D2 domain of the large subunit rRNA gene sequence similarity, 98 strains were identified to be of 36 yeast species in 18 genera belonging to Ascomycota viz. Candida, Clavispora, Cyberlindnera, Debaryomyces, Hanseniaspora, Hyphopichia, Kazachstania, Kluyveromyces, Kodamaea, Lachancea, Metschnikowia, Meyrozyma, Pichia, Starmerella, Torulaspora and Wickerhamomyces, and to Basidiomycota viz. Sporidiobolus and Trichosporon. Three strains were found to represent two novels Candida species which were previously described as C. sirachaensis and C. sakaeoensis. Ten strains of yeast-like fungus were identified as Aureobasidium pullulans of the phylum Ascomycota. Ascomycetous yeast species accounted altogether for 98.0 % of the 98 strains. The prevalent species was Candida tropicalis with a low frequency of isolation (14.3 %). Diversity of yeasts other than ballistoconidium-forming yeast in phylloplane in a tropical country in Asia has been reported for the first time. All strains obtained were accessed for the capability to produce IAA and result revealed that 39 strains in 20 species, one strain each of an undescribed and a novel species, and two unidentified strains showed the capability of producing IAA when cultivated in yeast extract peptone dextrose broth supplemented with 0.1 % L-tryptophan. All five strains of Candida maltosa produced relatively high concentrations of IAA.

Seasonal variations in the prevalence ofCryptococcus neoformansvar.grubiiandCryptococcus gattiiin decayed wood inside trunk hollows of diverse tree species in north-western India: a retrospective study

This study presents a 7-year retrospective analysis of seasonal variations in the prevalence of Cryptococcus neoformans var. grubii and Cryptococcus gattii in decayed wood inside trunk hollows of 518 trees belonging to 20 species in north-western India during 2000-2007. Of the 1,439 wood samples investigated, 406 (28.2%) were found to be positive for the Cryptococcus neoformans species complex which included 247 samples from which C. neoformans var. grubii was recovered and 171 which yielded C. gattii. While both of the pathogens were isolated through all the seasons, the overall prevalence of C. neoformans var. grubii was significantly higher (17.2%) than that of C. gattii serotype B (11.9%, P < 0.0001), indicating that decayed wood was as good, if not better, a natural habitat of C. neoformans var. grubii as that of C. gattii. The highest recovery of both yeasts was in the autumn, followed by that in the summer. For C. gattii, the lowest prevalence occurred during the winter and for C. neoformans var. grubii during the rainy season. The low prevalence of C. gattii during winter is similar to that reported from Bogota, Colombia, where C. gattii had a low population density in bark samples but it was not found in decayed wood of trunk hollows investigated during the period of January and February. The prevalence of C. neoformans var. grubii was significantly lower in the rainy season than in the other portions of the year. This finding is similar to the reported low isolation frequency (4%) of C. neoformans var. grubii from chicken feces in the rainy season in northern Thailand. Further investigations are warranted to determine the clinical significance of seasonal variations in the prevalence of C. neoformans var. grubii and C. gattii in decayed trunk wood of various trees in climatically divergent regions of India.

Lessons Learned from the Anaerobe Survey: Historical Perspective and Review of the Most Recent Data (2005–2007)

The rationale and lessons learned through the evolution of the National Survey for the Susceptibility of Bacteroides fragilis Group from its initiation in 1981 through 2007 are reviewed here. The survey was conceived in 1980 to track emerging antimicrobial resistance in Bacteroides species. Data from the last 11 years of the survey (1997-2007), including 6574 isolates from 13 medical centers, were analyzed for in vitro antimicrobial resistance to both frequently used and newly developed anti-anaerobic agents. The minimum inhibitory concentrations of the antibiotics were determined using agar dilution in accordance with Clinical and Laboratory Standards Institute recommendations. The analyses revealed that the carbapenems (imipenem, meropenem, ertapenem, and doripenem) and piperacillin-tazobactam were the most active agents against these pathogens, with resistance rates of 0.9%-2.3%. In the most recent 3 years of the survey (2005-2007), resistance to some agents was shown to depend on the species, such as ampicillin-sulbactam against Bacteroides distasonis (20.6%) and tigecycline against Bacteroides uniformis and Bacteroides eggerthii ( approximately 7%). Very high resistance rates (>50%) were noted for moxifloxacin and trovafloxacin, particularly against Bacteroides vulgatus. During that period of study, non-B. fragilis Bacteroides species had >40% resistance to clindamycin. Metronidazole-resistant Bacteroides strains were also first reported during that period. In summary, resistance to antibiotics was greater among non-B. fragilis Bacteroides species than among B. fragilis and was especially greater among species with a low frequency of isolation, such as Bacteroides caccae and B. uniformis. The emergence of resistance among the non-B. fragilis Bacteroides species underscores the need for speciation of B. fragilis group isolates and for clinicians to be aware of associations between species and drug resistance.

Low occurrence of patulin- and citrinin-producing species isolated from grapes

To assess the ability of fungi isolated from grapes to produce patulin and citrinin. A total of 446 Aspergillus isolates belonging to 20 species and 101 Penicillium isolates were inoculated in Czapek yeast extract agar and yeast extract sucrose agar and incubated for 7 days at 25 degrees C. Extracts were analysed for patulin and citrinin by thin-layer chromatography. None of the isolates of Aspergillus spp. produced either patulin or citrinin. Patulin was produced by three isolates of Penicillium expansum and two of Penicillium griseofulvum. Citrinin was produced by five isolates of P. expansum, two of Penicillium citrinum and one of Penicillium verrucosum. Our results show that the Aspergillus and Penicillium species commonly isolated from grapes are not a source of the mycotoxins, patulin and citrinin. The possibility of co-occurrence of patulin and citrinin with ochratoxin A in grapes and grape products remain low, owing to the low frequency of isolation of potentially producing species.

RF MEMS Switches and Integrated Switching Circuits

Radio frequency (RF) microelectromechanical systems (MEMS) have been pursued for more than a decade as a solution of high-performance on-chip fixed, tunable and reconfigurable circuits. This paper reviews our research work on RF MEMS switches and switching circuits in the past five years. The research work first concentrates on the development of lateral DC-contact switches and capacitive shunt switches. Low insertion loss, high isolation and wide frequency band have been achieved for the two types of switches; then the switches have been integrated with transmission lines to achieve different switching circuits, such as single-pole-multi-throw (SPMT) switching circuits, tunable band-pass filter, tunable band-stop filter and reconfigurable filter circuits. Substrate transfer process and surface planarization process are used to fabricate the above mentioned devices and circuits. The advantages of these two fabrication processes provide great flexibility in developing different types of RF MEMS switches and circuits. The ultimate target is to produce more powerful and sophisticated wireless appliances operating in handsets, base stations, and satellites with low power consumption and cost.