Low Exposure Research Articles

ABCB1 attenuates brain exposure to the KRASG12C inhibitor opnurasib whereas binding to mouse carboxylesterase 1c influences its plasma exposure

Opnurasib (JDQ443) is a newly developed oral KRASG12C inhibitor, with a binding mechanism distinct from the registered KRASG12C inhibitors sotorasib and adagrasib. Phase I and II clinical trials for opnurasib in NSCLC are ongoing. We evaluated the pharmacokinetic roles of the ABCB1 (P-gp/MDR1) and ABCG2 (BCRP) efflux and OATP1 influx transporters, and of the metabolizing enzymes CYP3A and CES1 in plasma and tissue disposition of oral opnurasib, using genetically modified cell lines and mouse models. In vitro, opnurasib was potently transported by human (h)ABCB1 and slightly by mouse (m)Abcg2. In Abcb1a/b- and Abcb1a/b;Abcg2-deficient mice, a significant ∼100-fold increase in brain-to-plasma ratios was observed. Brain penetration was unchanged in Abcg2-/- mice. ABCB1 activity in the blood-brain barrier may therefore potentially limit the efficacy of opnurasib against brain metastases. The Abcb1a/b transporter activity could be almost completely reversed by co-administration of elacridar, a dual ABCB1/ABCG2 inhibitor, increasing the brain penetration without any behavioral or postural signs of acute CNS-related toxicity. No significant pharmacokinetic roles of the OATP1 transporters were observed. Transgenic human CYP3A4 did not substantially affect the plasma exposure of opnurasib, indicating that opnurasib is likely not a sensitive CYP3A4 substrate. Interestingly, Ces1-/- mice showed a 4-fold lower opnurasib plasma exposure compared to wild-type mice, whereas no strong effect was seen on the tissue distribution. Plasma Ces1c therefore likely binds opnurasib, increasing its retention in plasma. The obtained pharmacokinetic insights may be useful for further optimization of the clinical efficacy and safety of opnurasib, and might reveal potential drug-drug interaction risks.

Positional Differences in Decision-Making Situations during Professional Rugby League Match-Play

The aim of this study was to explore the types and frequency of decision-making situations of rugby league players during defensive situations and examine whether they were predictive of key performance indicators (KPI). Fifteen elite rugby league matches were coded using notational analysis methods. Specific defensive situations were analysed, including the number of: one-on-one situations with an opposing attacker (1-on-1), two-on-one situations (2-on-1), and combined 1-on-1 and 2-on-1 situations (i.e., total decisions; TDs). There was no relationship between the game outcome and game KPIs for TDs or 1-on-1 decision-making situations. However, successful tackles and missed tackles were predictive of 2-on-1 decision-making situations. Positional differences revealed that back rowers were exposed to the greatest number of decision-making situations, while wingers had the lowest exposure. The total number of decisions and the number of 1-on-1 decisions made by the centres and wingers were significant predictors of line breaks. Additionally, 2-on-1 decisions were significant predictors of line breaks for backrowers. The findings of this study suggest that the type and frequency of decision-making situations in Rugby League are position specific. Practical applications for coaches are discussed to ensure that practice approaches are representative of the various defensive decision-making demands players experience during a game, based on their position.

Exposure to sublethal levels of H2S does not influence the short-term growth or feed utilization in post-smolt Atlantic salmon (Salmo salar) during restrictive feeding

Hydrogen sulfide (H2S) related mass mortality events in land-based recirculating aquaculture systems (RAS) are associated with the sudden release of substantial amounts of H2S, resulting in acutely toxic exposure. However, the production and release of H2S can also occur at slower constant rates, leading to continuous exposure to sublethal H2S concentrations, the effects of which are unknown. Here, we examined growth rates, feed conversion ratios, and apparent nutrient digestibility in post-smolt Atlantic salmon, following a 10-day exposure to one of two sublethal concentrations of H2S, compared against a control group. The H2S concentrations of the low exposure (LE) and moderate exposure (ME) groups throughout the trial were 1.8 ± 0.8 μg/L and 4.1 ± 1.9 μg/L, respectively. Neither exposure regime affected growth, feed utilization, or the apparent nutrient digestibility. The results suggest that Atlantic salmon tolerate exposure to sublethal H2S concentrations for 10 days, without a discernible effect on the production performance. For aquaculture systems, specifically RAS, this means that while higher concentrations of H2S should be strictly avoided, the presence of low H2S concentrations for a limited period might not be detrimental to fish welfare or production. The RAS environment is complex, and the potential combined effects of H2S and factors pertinent to RAS may influence fish performance differently in a production setting.

Sleep patterns in Metropolitan and Regional areas in Japan: Before and during COVID-19 social restrictions

ABSTRACT Sleep timing is an important output of the circadian system. The COVID-19-mandated social restrictions significantly altered commuting time and sleep duration regionally in Japan. This study aimed to elucidate sleep patterns, especially chronotype and social jetlag (SJL), due to changes in social time pressure through the social restrictions between the Metropolitan and Regional areas in Japan. As part of the Global Chrono Corona Survey 2020 (GCCS), the data were collected during social restrictions (SR), but pre-COVID-19 behaviours were also queried retrospectively. We analyzed a cohort of 729 respondents representing both the Metropolitan and the Regional areas separately for workdays and work-free days. While the areas showed no difference in SJL before SR, the differential decrease was larger in the Metropolitan area during SR, resulting in a significant difference in SJL between the areas. The outdoor light exposure before SR was 30 min longer in the Metropolitan areas than in the Regional; during SR both areas showed similarly low (below 1 h) outdoor light exposures. The variables associated with decreased SJL were the Metropolitan areas, work-from-home, a no-usage alarm clock on workdays, and chronotypes (mid-sleep time on free days corrected for sleep deficit accumulated over the workweek, MSFsc) during SR. The results suggest that relaxed social schedules, as reflected in the increased frequency of work-from-home and reduced alarm clock use, and moving towards earlier MSFsc during SR were linked to decreased SJL and were more prominent in the Metropolitan areas. This study provides insights into sleep patterns and the social time pressure markers, by comparison between residential groups in Japan.

Insights into tissue accumulation, depletion, and health risk assessment of clopidol in poultry.

Clopidol is extensively used in livestock farming and residues of this antibiotic can persist in animal tissues, posing a risk to humans and the environment. In this study, we investigated the depletion of clopidol in various edible tissues of chickens (muscle, liver, kidney, fat, and eggs) using liquid chromatography-tandem mass spectrometry after the administration of a clopidol-contaminated diet (at 250 mg kg-1 for the high (1x) dose). After 14 d of exposure, the clopidol concentrations were highest in eggs (median: 9.83 mg/kg), followed by liver (3.56 mg/kg), kidney (3.01 mg/kg), muscle (1.56 mg/kg), and fat (0.727 mg/kg) at low exposure group, indicating that clopidol accumulated primarily in eggs rather than the other edible tissues. In addition, the maternal transfer ratios were estimated, and the transfer efficiencies of clopidol in muscle (egg-to-tissue ratio, ETR:1.81) and fat (2.06-58.2) were higher than those in liver (0.731-31.1) and kidney (0.832-38.9). Furthermore, we conducted a cumulative risk assessment for clopidol in edible chicken tissues using the hazard quotient (HQ) method. This assessment revealed that the exposure levels for Korean consumers pose an acceptable risk. However, for eggs from the 1x dose exposure group, the HQ values were greater than 1 for all age groups, particularly for young children (<18 y), suggesting that the higher daily consumption of eggs combined with the higher clopidol residues in eggs resulted in higher HQ values, which requires further attention. The findings of this study can assist in the management and monitoring of clopidol residues in chicken tissues and eggs.

Patterns and socioeconomic differences in secondhand exposure to cigarettes, e-cigarettes, and heated tobacco products at home in Hong Kong adolescents.

Smoke or aerosols from cigarettes, e-cigarettes (ECs), or heated tobacco products (HTPs) are harmful. Yet, there is little knowledge about the specific patterns of secondhand tobacco exposure by source within household settings and the socioeconomic status (SES) differences in adolescents. We used territory-representative student data from a cross-sectional school-based survey in 2020-2021 to calculate the weighted prevalence of secondhand exposure to cigarettes, e-cigarettes, and HTPs in the past seven days. Parental education and perceived family affluence were used as indicators of socioeconomic status. Generalized linear mixed models were used to analyze associations. Among 22039 participants, 29.8% reported any secondhand tobacco exposure (SH-Any) at home, primarily from cigarettes (27.4%), followed by e-cigarettes (4.0%) and HTPs (0.9%). Tertiary parental education level was associated with lower SH-Any exposure (Adjusted odds ratio, AOR=0.49; 95% CI: 0.45-0.53, p<0.001), fewer exposure days (β= -0.685, p<0.001), lower exposure to cigarettes (SH-CC) (AOR=0.49; 95% CI: 0.45-0.54, p<0.001) and to e-cigarettes or HTPs (SH-EC/HTP) (AOR=0.57; 95% CI: 0.45-0.71, p<0.001). 'Poor' family affluence was associated with higher exposures [AOR(SH-Any) =1.14; 95% CI: 1.06-1.22, p=0.001; β(days)=0.160, p<0.001; AOR(SH-CC) =1.15; 95% CI: 1.07-1.24, p<0.001], except for SH-EC/HTP exposure, which was higher in students in an affluent family (AOR =1.66; 95% CI: 1.25-2.21, p<0.001). Significant SES differences in SH-EC/HTP exposure were found only in groups with low parental education level. Dose-response relationships were found between lower SH-Any and SH-CC and higher SES categories (p for trend<0.001). Adolescents experienced a high prevalence of tobacco smoke exposure at home, primarily from cigarettes. Higher SES was associated with lower tobacco exposure, except for SH-EC/HTP, which was higher among adolescents from affluent families. Additionally, high parental education level was protective against exposure to SH-EC/HTP. Comprehensive control measures to reduce the use of these tobacco products are needed to protect adolescents of diverse socioeconomic backgrounds.

A clinical comparative study of dual LVIS devices and single flow diversion stenting for the treatment of unruptured V3-V4 vertebral artery dissection.

This study aims to compare the efficacy and safety of using overlapping low-profile visualized intraluminal support (LVIS) devices and flow diversion (FD) for the treatment of unruptured vertebral artery dissection (VAD) in the V3-V4 segments. The clinical and imaging data of 71 patients with unruptured VAD in the V3-V4 segments who underwent either dual LVIS stenting (d-LVIS group) or single FD stenting (FD group) at our center from September 2014 to December 2021 were retrospectively analyzed. Immediate postoperative angiography revealed no significant difference in the degree of occlusion between the two groups in treating vertebral artery dissecting aneurysms (with or without noncompact coiling). However, the d-LVIS group had significantly higher fluoroscopy exposure time and total radiation exposure dose compared to the FD group. During the perioperative period, two cases of pontine infarction and one case of acute thrombosis were encountered. One patient died from subarachnoid hemorrhage during the follow-up period. For dissecting the aneurysm, angiographic follow-up (8.56 ± 1.96 months) showed similar healing outcomes between the two groups (with or without noncompact coiling). However, seven patients (7/40, 17.5%) showed poor healing and one patient showed mild in-stent stenosis. For simple dissection, angiographic follow-up (8.78 ± 1.83 months) showed patent lumens in both groups, with all dissections healing well, and two patients having mild in-stent stenosis. Both methods could effectively treat unruptured VAD in V3-V4 segments. Nevertheless, simple FD implantation is relatively easier to perform and involves lower radiation exposure.

Exposure assessment and risks associated with wearing silver nanoparticle-coated textiles

Background Silver (Ag) nanoparticles (NPs) are used increasingly in consumer and healthcare fabrics due to their antimicrobial properties. Abrasive leaching experiments have shown that AgNPs can be released during textile wear and cause a dermal exposure. Derived-no-effect-limit value for AgNPs ranges from 0.01 to 0.0375 mg/kg-body-weight, and thus, low exposures levels can cause relevant risk. Methods In this study AgNP release from textiles by artificial sweat immersion and mechanical stress was investigated. A mass balance model was used to calculate dermal Ag exposure and potential intake via percutaneous absorption and inadvertent (peri-)oral intake during wear of face mask, suit with a full body exposure and gloves. Mass flow analysis was performed for up to 8-h wear time and by using Ag penetration rate constants reported for fresh-, cryopreserved- and glycerolized skin grafts. Results Dermal intake risk characterization ratio (RCR) during 8-h wear time for glycerolized skin was up to 0.02 for face mask and 0.9 for full body wear in a worst-case condition. Wearing gloves for 1-h followed by single unintentional fingertip mouthing (contact area 11.5 cm2) resulted in an RCR of 0.0002. RCR varied depending on the type of textile-product, exposure wear duration and skin type. Conclusions This study provides a comprehensive assessment of AgNPs release from textiles and their potential impact on human dermal exposure and was essential for understanding the safety implications for different exposure scenarios and mitigating potential risks.

Pharmacokinetics of idelalisib in chronic lymphocytic leukemia and follicular lymphoma disclose better outcomes for patients with lower exposure

Pharmacokinetics of idelalisib in chronic lymphocytic leukemia and follicular lymphoma disclose better outcomes for patients with lower exposure

Evaluation and SAR Analysis of Low Frequency and Broadband Electric Field Exposure Measurement Values in the Home Environment

In this study, the effective value of low-frequency (50 Hz) and high-frequency (700–2500 MHz) broadband electric field exposures was measured for 24 h in four different selected environments in a house. All the statistical values of 1000 data points recorded with two measuring devices for 24 h in each environment are calculated, the most appropriate curves are fitted to the data, and the curves are plotted by expressing their changes with respect to time. All statistical and parametric values of the density and cumulative probability functions of the curves are calculated and plotted. In broadband measurements, the broadband is divided into fifteen sub-bands, but the data are available in only six of these sub-bands. The six-layer human head model is created by using the middle frequencies of the six sub-bands used, and taking the permittivity (εr) and conductivity (σ) of each layer into consideration. The specific absorption rate (SAR) value in the brain is calculated by using the total transmission coefficient of six layers. The SAR value surrounding the head is obtained. These SAR values are interpreted by considering the Federal Communications Commission (FCC) and the Community European (CE) occupational and general public SAR limits.

Association between secondhand smoke exposure and rheumatoid arthritis in US never-smoking adults: a cross-sectional study from NHANES

While smoking is widely acknowledged as a risk factor for rheumatoid arthritis (RA), the connection between secondhand smoke (SHS) exposure and RA in never-smoking adults remains limited and inconsistent. This study aims to explore and quantify this association using serum cotinine levels. We conducted a cross-sectional study with 14,940 adults who self-report as never smokers, using National Health and Nutrition Examination Survey data from 1999 to 2018. Based on previous literature, SHS exposure was categorized into four groups according to serum cotinine levels. Compared to individuals in the unexposed group (serum cotinine < 0.05 ng/mL), the adjusted odds ratio (OR) for RA was 1.37 (95% CI 1.14–1.64, p = 0.001) in the low exposure group (serum cotinine at 0.05 to 0.99 ng/mL) after adjusting for covariates. However, no significant association was found in the moderate exposure group (serum cotinine at 1 to 10 ng/mL) or the heavy exposure group (serum cotinine ≥ 10 ng/mL). Furthermore, we detected a non-linear, positively saturated correlation between the cotinine levels after log2 transformation and RA, with a turning point at approximately − 2.756 ng/mL (OR = 1.163, 95% CI 1.073–1.261, p = 0.0002). The stability of the results was confirmed by subgroup analysis.

Recreational music exposure and hearing health in young adults.

This study aimed to compare daily and total recreational music exposure levels and extended-spectrum audiogram results in young adults without pre-existing hearing problems. The study included healthy volunteers aged 18-25 with no known ear disease or hearing loss. Participants completed a questionnaire, underwent otoscopic and tympanometric examinations, and determined preferred music volumes in an audiometry booth using calibrated music samples of their preferred genres. Hearing thresholds up to 16kiloHertz (kHz) were measured. Daily music exposure for each participant was normalized to 8h to calculate a time-weighted average of 8h (TWA8). Total exposure (TE) was calculated by multiplying TWA8 by the number of years of music listening. A total of 32.4% of participants had TWA8s above 65dB. Their hearing thresholds at 125, 250, 500, and 16,000Hz and the average of 125Hz-8kHz were significantly higher. Participants with TWA8s above 65dB were also more prone to speaking loudly and experiencing communication difficulties on the phone. Those with a TE of more than 400 experienced significantly more speech discrimination difficulty in noisy environments and temporary hearing loss/tinnitus after exposure to loud music. Participants with a TE above 700 had worse thresholds at 4, 14, and 16kHz frequencies, as well as 125-8000Hz and 500-4000Hz averages compared to those with a TE below 700. This study provides evidence that recreational music with much lower exposure levels than the universally accepted TWA8 of 85dB could negatively impact hearing in healthy young adults. Therefore, maintaining a maximum TWA8 of 65dB is recommended.

The role of membrane transporters in the absorption of atrazine following nasal exposure

Objective The purpose of these studies was to investigate the uptake of atrazine across the nasal mucosa to determine whether direct transport to the brain through the olfactory epithelium is likely to occur. These studies were undertaken to provide important new information about the potential for the enhanced neurotoxicity of herbicides following nasal inhalation. Materials and Methods Transport of atrazine from aqueous solution and from commercial atrazine-containing herbicide products was assessed using excised nasal mucosal tissues. The permeation rate and the role of membrane transporters in the uptake of atrazine across the nasal mucosa were also investigated. Histological examination of the nasal tissues was conducted to assess the effects of commercial atrazine-containing products on nasal tissue morphology. Results Atrazine showed high flux across both nasal respiratory and olfactory tissues, and efflux transporters were found to play an essential role in limiting its uptake at low exposure concentrations. Commercial atrazine-containing herbicide products showed remarkably high transfer across the nasal tissues, and histological evaluation showed significant changes in the morphology of the nasal epithelium following exposure to the herbicide products. Discussion Lipophilic herbicides such as atrazine can freely permeate across the nasal mucosa despite the activity of efflux transporters. The adjuvant compounds in commercial herbicide products disrupt the nasal mucosa’s epithelial barrier, resulting in even greater atrazine permeation across the tissues. The properties of the herbicide itself and those of the formulated products play crucial roles in the potential for the enhanced neurotoxicity of herbicides following nasal inhalation.

Enteroparasites and risk factors for infection in native and exotic domiciled birds in the North Pioneer region of Paraná State, Brazil

Although parasitic infections are common in free-ranging birds, they pose more significant threats in captivity. Free-ranging birds can live in large areas and have low exposure to parasitosis. On the other hand, birds kept in captivity, the high population density results in greater exposure, putting the animal's survival at risk. The aim of this study was to assess the occurrence of gastrointestinal endoparasites in domiciled birds (native and exotic) in the Mesoregion North Pioneer of Paraná. For this, 59 fecal samples were collected from 63 birds and analyzed by the centrifugation-flotation method in hypersaturated sucrose solution. Of the 63 birds, 38 (60%) were native, 22 (35%) exotic and 3 (5%) hybrids. Oocysts, eggs and larvae were found in 22.03% (13/59) of the samples analyzed. Higher occurrence of protozoa (20.34% - 12/59) than helminths (5.08% - 3/59) was observed, in which seven genera found: Isospora, Eimeria, Ancylostoma, Heterakis, Strongyloides, Cryptosporidium and one cestode. No endoparasites were found in the ten exotic birds and three native birds analyzed. It is concluded that the birds were infected by helminths and protozoa with a higher occurrence of coccidia, with the genus Isospora predominating. Probably due to the low sampling, there was no influence of keeping birds in individual cages compared to birds kept in collective cages, birds raised in rural or urban areas, nor of the adequacy and hygiene of drinkers and feeders, thermal comfort and hygiene of the birds' enclosures. Studies on parasites in wild birds are necessary to gain a more profound understanding of the intensity of anthropogenic influences on these animal species.

Assessment of body mass-related covariates for rifampicin pharmacokinetics in healthy Caucasian volunteers.

Currently, body weight-based dosing of rifampicin is recommended. But lately, fat-free mass (FFM) was reported to be superior to body weight (BW). The present evaluation aimed to assess the influence of body mass-related covariates on rifampicin's pharmacokinetics (PK) parameters in more detail using non-linear mixed effects modeling (NLMEM). Twenty-four healthy Caucasian volunteers were enrolled in a bioequivalence study, each receiving a test and a reference tablet of 600mg of rifampicin separated by a wash-out period of at least 9days. Monolix version 2023R1 was used for NLMEM. Monte Carlo simulations (MCS) were performed to visualize the relationship of body size descriptors to the exposure to rifampicin. A one-compartment model with nonlinear (Michaelis-Menten) elimination and zero-order absorption kinetics with a lag time best described the data. The covariate model including fat-free mass (FFM) on volume of distribution (V/F) and on maximum elimination rate (Vmax/F) lowered the objective function value (OFV) by 56.4. The second-best covariate model of sex on V/F and Vmax/F and BW on V/F reduced the OFV by 51.2. The decrease in unexplained inter-individual variability on Vmax/F in both covariate models was similar. For a given dose, MCS showed lower exposure to rifampicin with higher FFM and accordingly in males compared to females with the same BW and body height. Our results indicate that beyond BW, body composition as reflected by FFM could also be relevant for optimized dosing of rifampicin. This assumption needs to be studied further in patients treated with rifampicin.

Variation in metabolic rate during low tide aerial exposure in the Asian shore crab Hemigrapsus sanguineus

Intertidal animals deal with physical gradients daily that create stressful conditions across the shore. These physical gradients influence the physiological performance of organisms, requiring responses that may differ with height on the shore. We examined the respiratory response to aerial exposure in the invasive Asian shore crab Hemigrapsus sanguineus during periods of low tide emersion using two field experiments. The first experiment simultaneously measured respiration of individuals collected from different heights on the shore, which had therefore been emersed for different lengths of time. The second experiment measured respiration of individuals collected at different times from the same tidal height. Respiration rates of crabs in both experiments increased immediately after emersion, nearly doubling by and peaking at ~ 1.5 h of aerial exposure, before decreasing again over the next 1.5 h. These results suggest that the energetic cost of low tide exposure is greatest shortly after emersion during the first half of the typical low tide period, but then decreases thereafter. These respiration patterns facilitate the broad intertidal distribution of this species on rocky shores throughout its range.

Comprehensively evaluating the performance of Elekta high-definition dynamic radiosurgery for hypofractionated stereotactic radiotherapy of multiple brain metastases

PurposeTo evaluate the dosimetric and delivery performance of Elekta high-definition dynamic radiosurgery (HDRS) in hypofractionated stereotactic radiotherapy (HSRT) for multiple brain metastases (BMs) by comparison with a conventional single-isocenter noncoplanar volumetric modulated arc therapy (VMAT) technique using the RayStation treatment planning system (TPS). Materials and methodsFrom June 2022 to October 2023, thirty-two patients (a total of 140 BMs) treated with HSRT (30–48 Gy/3-6f) via HDRS-based single-isocenter noncoplanar VMAT (HDRS-VMAT) were retrospectively selected for this study. Each patient was replanned using RayStation TPS-based single-isocenter noncoplanar VMAT (RayStation-VMAT). HDRS-VMAT and RayStation-VMAT plans were compared in terms of the gradient index (GI), conformity index (CI), normal brain tissue (NBT) dose exposure, monitor units (MUs), homogeneity index (HI), delivery accuracy, and beam-on time (BOT). ResultsCompared with the RayStation-VMAT plan, the HDRS-VMAT plan achieved superior CI (1.14 ± 0.09 vs. 1.23 ± 0.12, P < 0.001) and GI (5.23 ± 1.18 vs. 7.83 ± 2.75, p < 0.001) but had greater HI values. Compared with the RyaStation-VMAT plan, the HDRS-VMAT plan also yielded significantly lower NBT dose exposures (V23Gy, V21Gy, V18Gy, V12Gy and Dmean) but at the cost of increased MUs (3659.16 ± 1225.28 vs. 2536.23 ± 785.80, p < 0.001). The difference in BOTs was minimal (<1 min), which was not considered clinically significant. Furthermore, the HDRS-VMAT exhibited a higher gamma passing rate across all criteria (P < 0.001), particularly under stricter criteria (2%/1 mm), achieving an approximately 3% increase. ConclusionCompared with RaySation-VMAT, HDRS-VMAT offers superior target conformity, better NBT sparing, and higher delivery accuracy while maintaining comparable delivery efficiency. This represents a promising approach for HSRT of multiple BMs.

An investigation into the effect of UV irradiation and biofilm colonization on adsorption and desorption behavior of polyurethane (PU) microplastics for bisphenol A (BPA)

Recent studies have demonstrated that microplastics (MPs) can act as a transport vector for pollutants in the environment. However, most studies have focused on carbon-carbon backbone polymers, with little known about heteroatom backbone polymers. In this study, polyurethane (PU) MPs were modified by UV irradiation and microorganism colonization to assess the different aging treatments on the interaction behavior (adsorption/desorption) between PU MPs and bisphenol A (BPA). The results of the adsorption kinetics experiment illustrated that the adsorption process of BPA could be described well by a pseudo-second order kinetic equation on both fresh and aged PU MPs. Although fresh PU MPs revealed the highest saturated adsorption capacity, at low BPA exposure concentrations (≤ 100 μg/L) the adsorption capacities for UV aged and biofilm colonized PU MPs were very similar to fresh MPs or even slightly higher than for fresh MPs, the main adsorption mechanisms including H-bonding interactions and π–π interactions. Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) investigations suggested obvious physicochemical property changes (e.g., generation of new functional groups, erosion, etc.) after UV irradiation and biofilm colonization. This study also showed that different exposure environments (ultrapure water, artificial seawater, simulated gastric fluid) could affect BPA adsorption/desorption via hydrophobic and electrostatic interaction on PU surfaces. These results further clarify the adsorption mechanisms of BPA on PU MPs that have undergone different aging treatments, and provides a theoretical basis for the assessment of environmental behavior and exposure risks when PU MPs and BPA coexist.

Investigating the temperature dependence of helium bubble dynamics in plasma exposed tungsten via in-situ TEM annealing

In-situ TEM annealing and imaging were performed on tungsten samples pre-exposed at either 573 K or 1013 K in a pure helium plasma to a fluence of 1025 m−2. To investigate the thermal evolution of helium bubbles, the samples were imaged for annealing temperatures up to 998 K in steps of 100 K. It is found that annealing temperatures had little effect on the bubble dynamics for the lower temperature exposure of 573 K. Elongated bubble structures remained in the sample throughout the annealing process and there is minimal change in both the average bubble size and cross-sectional number density. However, annealing had a significant effect on the higher temperature exposure of 1013 K. Bubbles were found to be trapped in large clusters and increasing annealing temperature caused a significant increase in the average bubble size accompanied by a decrease in the cross-sectional bubble number density. The experimental observations are supported by theoretical calculations that show a simultaneous increase in the average number of He atoms per bubble in the higher exposure temperature sample but an overall decrease in He atoms retained within bubbles as the annealing temperature increased. These trends in bubble dynamics with annealing temperature suggest that Ostwald ripening dominates, whereby bubble growth is determined by the different pressures of different bubble sizes, rather than through bubble migration and coalescence.

Augmented fluoroscopy-guided dye localization for small pulmonary nodules in hybrid operating room: intrathoracic stamping versus transbronchial marking.

We developed a novel augmented fluoroscopy-guided intrathoracic stamping technique for localizing small pulmonary nodules in the hybrid operating room. We conducted an observational study to investigate the feasibility of this technique and retrospectively compared two augmented fluoroscopy-guided approaches: intrathoracic and transbronchial. From August 2020 to March 2023, consecutive patients underwent single-stage augmented fluoroscopy-guided localization under general anaesthesia. This included intrathoracic stamping and bronchoscopic lung marking, followed by thoracoscopic resection in a hybrid operating room. Comparative analyses were performed between the two groups. The data of 50 patients in the intrathoracic stamping and 67 patients in the bronchoscopic lung marking groups were analysed. No significant difference was noted in demographic data between the groups, except a larger lesion depth in the bronchoscopic lung marking group (14.7 ± 11.7 vs 11.0 ± 5.8mm, p = 0.029). Dye localization was successfully performed in 49 intrathoracic stamping group patients (98.0%) and 67 bronchoscopic lung marking group patients (100%). No major procedure-related complications occurred in either group; however, the time flow (total anaesthesia time/global operating room time) was longer, and the radiation exposure (fluoroscopy duration/total dose area product) was larger in the bronchoscopic lung marking group. Augmented fluoroscopic stamping localization under intubated general anaesthesia is feasible and safe, providing an alternative with less global operating room time and lower radiation exposure for image-guided thoracoscopic surgery in the hybrid operating room.