Low-carbohydrate Foods Research Articles

Replacement of Dietary Carbohydrate with Protein versus Fat Differentially Alters Postprandial Circulating Hormones and Macronutrient Metabolism in Dogs.

The effect of dietary macronutrients on fasting and postprandial responses was examined. Thirty-six healthy dogs were fed a high-carbohydrate (HiCHO) food once daily for 5 weeks, followed by randomization to either a high-protein, low-carbohydrate (PROT_LoCHO) or high-fat, low-carbohydrate (FAT_LoCHO) food for 5 weeks, then crossed over to the other LoCHO food for 5 weeks. Plasma samples were obtained at the end of each feeding period at timepoints before (0 h) and 2 h post-feeding. Apparent total circulating energy availability was assessed as a summation of the energetic contributions of measured glucose, β-hydroxybutyrate, triglycerides (TGs), non-esterified fatty acids (NEFAs), and fatty acids not from TGs or NEFAs. In both the fed and fasted states, there were increases in circulating apparent total energy availability after feeding the FAT_LoCHO food compared with the HiCHO or PROT_LoCHO foods. Changes from the postabsorptive to postprandial points in catabolic, anabolic, and signaling lipids all exhibited food effects. Consumption of either LoCHO food led to lower leptin/ghrelin ratios in the fasted state relative to the HiCHO food. The FAT_LoCHO food led to the highest postprandial levels of the incretins gastric inhibitory peptide and glucagon-like peptide-1, yet the lowest increases in insulin relative to the other foods. These findings provide information on how macronutrients can influence dietary energy processing and metabolic health.

Immune-Modulating Effects of Low-Carbohydrate Ketogenic Foods in Healthy Canines

BackgroundKetogenic foods limit digestible carbohydrates but contain high fat, and have antioxidant and anti-inflammatory effects as well as improving mitochondrial function. β-Hydroxybutyrate (BHB), 1 of the ketone bodies, reduces the proinflammatory NLR family pyrin domain containing 3 inflammasomes, as well as chemokines in cultures. ObjectivesWe assessed the immune-modulating effects of 2 low-carbohydrate (LoCHO) foods varying in protein and fat and compared their effects with a food replete with high-carbohydrate (HiCHO) in healthy canines. MethodsDogs were fed control food [HiCHO; ketogenic ratio (KR: 0.46) followed by LoCHO_PROT (KR: 0.97), then LoCHO_FAT (KR: 1.63) or LoCHO_FAT followed by LoCHO_PROT. Each food was fed for 5 wk, with collections in the 5th wk; 15 wk feeding total. Gene expression for circulating inflammatory cytokines from 10 dogs was assessed using the Canine RT2 Profiler polymerase chain reaction array, and fold changes were calculated using the ΔΔCt method. ResultsLoCHO_FAT significantly increased circulating β-hydroxybutyrate compared with both HiCHO and LoCHO_PROT. When compared with HiCHO, there was a significant decrease in several proinflammatory cytokines/chemokines in LoCHO_PROT and LoCHO_FAT groups, including chemokine (C-C motif) ligand (CCL)1, CCL8, CCL13, CCL17, CCL24, chemokine (C-X3-C motif) ligand 1, chemokine (C-X-C motif) receptor 1, Interleukin-10 receptor alpha ((IL)-10RA), IL-1 receptor antagonist, IL-5, and secreted phosphoprotein 1 (all P < 0.05). Interestingly, a subset of inflammatory proteins that decreased in LoCHO_PROT but not in LoCHO_FAT included IL-33, IL-6 receptor, IL-7, IL-8, Nicotinamide phosphoribosyltransferase, and tumor necrosis factor (TNF) receptor superfamily member 11B. In contrast, the decrease in inflammatory markers in LoCHO_FAT, but not in LoCHO_PROT, included complement component 5, granulocyte colony-stimulating factor or G-CSF, interferon-γ, IL-3, IL-10RB, IL-17C, Tumor necrosis factor superfamily (TNFSF)13, TNFSF13B, and TNFSF14. Decreased concentrations of selected cytokines indicate that both low-carbohydrate foods exert an anti-inflammatory effect and provide a strong rationale for testing their efficacy in dogs with inflammatory conditions. ConclusionsBoth LoCHO_PROT and LoCHO_FAT foods might be important as part of immune-modulating therapeutic nutritional strategies to reduce inflammation to maintain health in canines. Our study identifies several inflammatory genes that are reduced when fed ketogenic food that were not previously reported.

HUBUNGAN SCREEN TIME, KEBIASAAN MAKAN JUNK FOOD, AKTIVITAS FISIK DENGAN KEJADIAN OBESITAS PADA REMAJA DI MAN 14 JAKARTA TIMUR

Based on the results of the 2018 Basic Health Research, the prevalence of adolescents aged 15 years who have central obesity is 31.0%. Obesity occurs due to the income of certain population groups, and eating habits that have begun to shift to disproportionate foods such as low carbohydrate, high fat and low fiber foods, as well as less physical activity caused by busyness and screen time. The aim of this study was to analyze the relationship between screen time, junk food eating habits and physical activity with obesity in teenagers at MAN 14 East Jakarta. The research was conducted in May 2023 at Madrasah Aliyah Negeri 14 East Jakarta with a sample size of 57 respondents aged 16 - 17 years. The sampling technique uses simple random sampling. This research uses quantitative research methods with a cross sectional design. Data were collected using a screen time questionnaire, for junk food eating habits using the FFQ and physical activity using the physical activity level (PAL), then the data was analyzed using the chisquare test. The results of the study showed that there was a relationship between screen time (p= 0.000), junk food eating habits (p= 0.000), and physical activity (p= 0.032) with the incidence of obesity in adolescents. It is hoped that MAN 14 East Jakarta students can increase physical activity and reduce screen time habits and consumption of junk foodKeywords physical activity, Junk food, Screen Time, Obesity

GUEST`S PREFERENCES FOR DIFFERENT FOODS AT HOTEL’S BREAKFAST: RESULTS OF AN ON-LINE SURVEY

Consumer choice and satisfaction are very important for the hotel industry. For guests, food has a big impact on satisfaction and so does breakfast. The objective of this work is to know what the preferences of guests in a hotel breakfast are and how this varies according to their characteristics. An online questionnaire was sent using social media to hotel guests (n=626). Most respondents live in Europe (62%) and they prefer fruit juices (37%) and pastries (37%) while non-European residents suggested fruits (58%) and dairy products (39%) as the main preferences. Respondents aged over 55 years old have also preferences for fruits and dairy products, whereas guests aged 18-34 prefer pastries. For families traveling with children, bread was preferred for breakfast. Moreover, guests between the ages of 18 and 34 prefer lactose-free foods; and vegans or vegetarians or guests between the ages of 35 and 54 prefer gluten-free foods; whereas those 35 or younger prefer sugar foods. Most women prefer lactose-free food and non-Europeans give more importance to low-carbohydrate food while Europeans prefer lactose-free food and vegetarians or vegans. Breakfast is a meal valued by guests. Knowing the preferences of the guests following the profile of each one will be an asset for hotel projects that can satisfy and retain their customers. As far as we are aware, this is the first assessment of travellers about their preferences in hotels breakfast.

A very low-carbohydrate diabetes prevention program for veterans with prediabetes: a single-arm mixed methods pilot study.

All Veterans Affairs (VA) Medical Centers offer the MOVE! Weight Management Program to help patients achieve and maintain a healthy weight through a calorie-restricted, low-fat diet and increased physical activity. Yet, most MOVE! participants do not achieve clinically significant weight loss of ≥5%. A carbohydrate-restricted diet may help more Veterans to achieve ≥5% weight loss. This was a single-arm explanatory sequential mixed methods pilot study conducted in one VA health care system. Veterans with prediabetes and body mass index ≥25 kg/m2 were invited to participate in a group-based, virtual, very low-carbohydrate Diabetes Prevention Program (VLC-DPP) consisting of 23 sessions over 12 months. Participants were taught to follow a very low-carbohydrate eating pattern, defined as 20-35 grams of net carbohydrates per day. The primary outcomes were measures of feasibility and acceptability, including program uptake and session attendance. Secondary outcomes included change in weight, hemoglobin A1c, lipids, and patient-reported measures of food cravings, stress eating, perceived health status, and motivation. Interviews were conducted at 6 months to identify factors that facilitated or hindered participants' achievement of ≥5% weight loss. Among 108 screened Veterans, 21 enrolled in the study (19%), and 18 were included in the analytic cohort. On average, participants attended 12.4/16 weekly sessions and 3.6/8 bimonthly or monthly sessions. At 12 months, mean percent weight loss was 9.4% (SD = 10.7) with 9 participants (50%) achieving ≥5% weight loss. Three factors facilitated achievement of ≥5% weight loss among 10/16 interviewees: (1) enjoyment of low-carbohydrate foods; (2) careful monitoring of carbohydrate intake; and (3) reduced hunger and food cravings. Three factors hindered achievement of ≥5% weight loss among 6/16 interviewees: (1) food cravings, particularly for sweets; (2) challenges with maintaining a food log; and (3) difficulty with meal planning. A VLC-DPP is feasible and acceptable and shows preliminary efficacy among Veterans with prediabetes. The program's weight loss effectiveness compared to standard MOVE! should be evaluated in a larger-scale trial. Such a program may be offered in addition to the standard MOVE! program to expand the menu of evidence-based lifestyle counseling options for Veterans. https://clinicaltrials.gov/ct2/show/NCT04881890, identifier NCT04881890.

Physico-Biochemical Property Evaluation of Certain Promising Varieties of Chickpea (Cicer arietinum L.)

Chickpea (Cicer arietinum L.), often known as gramme or Bengal gramme, is a self-pollinated leguminous crop with diploid annual (2n = 16 chromosomes) that belongs to the family Leguminosae and subfamily Papilionaceae. During the Rabi season, it is the most significant crop in India. Chickpeas are a high-energy, high protein, and low carbohydrate food. Gram is a grain that is utilised for both human consumption and animal feed. It comes in flour, whole grain, roasted, and cocked varieties. The most common forms consumed by people are salted Dahl or sweet concoctions, as well as green foliage and grain as vegetables. In the Rabi season, 60% of pulses are grown, and 40% in the Kharif season. Chickpeas are high in calories, protein, minerals, vitamins, and fibre that may be useful to one's health. For biochemical characteristics on selected potential genotypes/varieties of chickpea [Cicer arietinum (L)], seeds of chickpea genotypes/varieties were obtained from legume Breeder, Department of Genetics and Plant breeding, CSAUAT, Kanpur. The experiment was planned in the Department's Research Laboratory in order to achieve the goals of their research. Dhal percent, husk percentages, broken dal, percentage loss in processing, protein, test weight, grain yield quintal/ha, were 71.40-82.92g, 7.50-17.74 percent, 2.00-5.60 percent, 20.85-23.95 percent, 21.45-27.70g, 21-30q/ha, respectively. In genotypes K3256, Avrodhi, KGD1296, KGD2021, K3256, K3256 of the varietal trail, lower husk percentage, broken percentage, and processing loss were identified, as well as greater nutritional elements such as protein content also identified.

Macronutrient Proportions and Fat Type Impact Ketogenicity and Shape the Circulating Lipidome in Dogs.

Many physiological processes including ketogenesis are similar in dogs and humans, but there is little information available on the effect of carbohydrate restriction in dogs. Here, the ketogenicity and serum metabolic profiles of dogs were assessed after they had consumed high carbohydrate (HiCHO); high protein, low carbohydrate (PROT_LoCHO); or high fat, low carbohydrate (FAT_LoCHO) foods. Thirty-six dogs were fed HiCHO for 4 weeks, then randomized to PROT_LoCHO or FAT_LoCHO for 5 weeks. Dogs then crossed over to the other food for an additional 5 weeks. Generally, reduction of dietary carbohydrate by replacement with either protein or fat increased the energy required to maintain body weight, and fat had a greater effect. Postabsorptive energy availability derived mainly from glucose and triglycerides with HiCHO, from gluconeogenic amino acids and fatty acids with PROT_LoCHO, and from fatty acids and β-hydroxybutyrate with FAT_LoCHO. This study demonstrated that the reduction of carbohydrate in canine foods is potentially beneficial to dogs based on improvements in metabolism and supports the use of low-carbohydrate foods as safe and effective for healthy adult dogs.

Abstract 735: Low-carbohydrate diet score and the risk of colorectal cancer: Findings from a prospective cohort study

Abstract Background. Colorectal cancer (CRC) is a commonly diagnosed cancer. Glucose intake enhances the secretion of insulin, may contribute to cell growth and proliferation, leading to CRC development. Epidemiologic studies have shown that individuals with diabetes history are at increased risk of CRC. A low-carbohydrate diet, represented by a low-carbohydrate diet (LCD) score, may reduce the risk of diabetes. Yet, little is known about the association between LCD scores and the risk of CRC. Methods. We evaluated the associations between LCD and CRC risk in the Singapore Chinese Health Study, a population-based prospective cohort study that includes 61,321 Chinese men and women in Singapore who were 45-74 years old at enrollment. Food and dietary supplement consumption were assessed by a validated semi-quantitative food frequency questionnaire. Participants were assigned a score from 0 to 10 according to their rankings by daily intake of calories from carbohydrates, fat and protein, respectively. A score of 0 was assigned to a subject ranked in the highest one-eleventh of calorie intake from carbohydrates or in the lowest one-eleventh of calories from fat or protein, respectively. In contrast, a score of 10 was given to each subject in the lowest one-eleventh of carbohydrate intake or the highest one-eleventh of fat or protein intake. The LCD score for each subject was the sum of the 3 individual ranking scores. The highest possible value was 30, which represented the lowest carbohydrate intake and the highest fat and protein. In addition, animal-based and plant-based LCDs were similarly created from the daily consumption of calories from animal or plant protein and fat, respectively, as well as carbohydrates. Cox proportional hazard regression models were used to estimate hazard ratios (HRs) and their 95% confidence intervals (CIs) for CRC according to higher quartiles of LCD scores with adjustment for potential confounders. Results. After an average of 17.5 years of follow-up, 2,140 participants developed CRC. Overall, there was a null association between total LCD and CRC risk. A moderate positive association was found for animal-based LCD with CRC risk. The multivariable-adjusted HRs(95%CIs) for CRC in quartiles 2, 3, and 4 of animal-based LCD were 1.12(0.99-1.26), 1.18(1.04-1.33), and 1.11(0.98-1.25)(Ptrend=0.06) compared with the lowest quartile. There were no statistically significant differences in the animal-based LCD and CRC risk in subgroups stratified by sex, body mass index, smoking status, and history of type 2 diabetes. Conclusions. This large population-based prospective cohort study shows that a high animal-based LCD score, which represented low intake of carbohydrates and high intake of animal protein and fat, was associated with ~10% increased risk of CRC. Our findings suggest that replacing low-carbohydrate food with animal-based food may increase the risk of developing CRC. Citation Format: Yi-Chuan Yu, Pedram Paragomi, Aizhen Jin, Renwei Wang, Robert E. Schoen, Woon-Puay Koh, Jian-Min Yuan, Hung N. Luu. Low-carbohydrate diet score and the risk of colorectal cancer: Findings from a prospective cohort study [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 735.

Ketogenic and Modified Mediterranean Diet as a Tool to Counteract Neuroinflammation in Multiple Sclerosis: Nutritional Suggestions.

Ketogenic Diet is a nutritional pattern often used as dietotherapy in inflammatory diseases, including neurological disorders. Applied on epileptic children since 1920, in recent years it has been taken into account again as a tool to both reduce inflammatory burdens and ameliorate the nutritional status of patients affected by different pathologies. Multiple sclerosis (MS) is considered an immune-mediated neuro-inflammatory disease and diet is a possible factor in its pathogenesis. The aim of this work is to investigate the main potential targets of MS-related impairments, in particular the cognitive deficits, focusing on the alteration of biomarkers such as the Brain Derived-Neurotrophic Factor and the Tryptophan/Kynurenine ratio that could play a role on neuroprotection and thus on MS progression. Furthermore, we here propose nutritional suggestions which are useful in the development of a ketogenic diet protocol that takes advantage of the anti-inflammatory properties of low-carbohydrate foods from the Mediterranean diet to be applied to subjects with MS. In conclusion, this approach will allow one to develop the ketogenic diet combined with a modified Mediterranean diet as a possible tool to improve neuroinflammation in multiple sclerosis.

1515-PUB: Opposite Effects of Low-Carbohydrate High-Fat Diet on Metabolism in Humans and Mice

Aims: Low-carbohydrate diet (LCD) , characterized by low consumption of carbohydrate and high intake of fat, is well known to decrease body weight and blood glucose in humans. However, in animal experiments, high-fat diet (HFD) is widely used to induce dietary obese model with metabolic disorders. The study aims to explore why LCD/HFD has the opposite effects on humans and animals. Methods: Twenty-two adults with overweight or obesity were enrolled to receive LCD for three weeks. The subjects were divided into two groups according to whether carbohydrate intake is below 10% by calories, and their weight loss and glucose homeostasis were compared. C57 mice were randomly assigned to five groups: chow diet (CD) , HFD with respectively 60%, 70% and 75% fat (cocoa butter) by calories and 60% fat (lard) by calories (60%HFD-L) , and metabolism of mice was evaluated. Results: In this trial, subjects who consumed less carbohydrate had greater weight loss and better glycemic control compared with the others. The questionnaires revealed that most of the subjects favored high-carbohydrate (HC) food rather than low-carbohydrate food. However, in mice, HFD groups showed more weight gain and higher blood glucose compared with CD group. When provided with CD and HFD at the same time, mice ate little CD. Besides, we found HFD group had lower mRNA level of Npy and Agrp and higher mRNA level of Pomc and Cartpt in hypothalamus in comparison with CD group. Among HFD with coco butter groups, mice took in more calories and gained more weight as the fat content in diet increases. Compared with HFD with coco butter groups, mice in 60%HFD-L group consumed even more calories, and had heavier body weight and poorer glucose metabolism. Conclusion: LCD has opposite effects on metabolism in humans and mice because of different food preferences. People prefer HC food while mice favor HFD. Mice consume more and feel less hungry when the diet contains higher proportion of fat, which leads to poorer metabolic status. Besides, mice prefer lard to coco butter. Disclosure X.Xia: None. L.Cai: None. Y.Gu: None. J.Yin: None. Funding National Natural Science Foundation of China (No. 82070885) ,Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Grant Support (No. 20172025)

Equivalent Glycemic Load (EGL) of Atkins Chocolate Peanut Butter Bar and Atkins Chocolate Ready-to-Drink Shake in People With Type 2 Diabetes

ObjectivesIt is difficult to estimate the glycemic impact of low-carbohydrate (low-CHO) foods from the information in the Nutrition Facts Table. We developed equivalent glycemic load (EGL) to quantify the glycemic impact of low-CHO foods in people without diabetes. The current objective was to determine the feasibility and safety of measuring EGL in people with type 2 diabetes (T2D). MethodsOvernight fasted adults with T2D treated by diet or oral agents and A1c < 8.1% consumed 1 serving of Atkins Chocolate Peanut Butter Bar (CPB) or Atkins Milk Chocolate Shake (MCS) or 45 g white bread (WB) in random order on 3 separate days. CPB/MCS, respectively, contained 150/160 kcal, 14/9 g fat, 16/15 g protein, 23/5 g CHO and 12/3 g fiber. We took finger-stick blood fasting and every 30 min for 3 h after starting to eat. Serum glucose was measured by glucometer (analytic CV = 8.6%) and by the Vitros Chemistry System (Vitros, CV = 1.6%). In each subject F/(WB/Cwb) was calculated where F = incremental area under the curve (iAUC) for the food, WB = iAUC after WB and Cwb = 20.8 (the amount of CHO in WB). EGL = mean F/(WB/Cwb), after excluding values >2 SD from the mean. ResultsWe screened 18 subjects; 4 were ineligible (A1c >8.0%), 14 were enrolled and 2 dropped out. The remaining 12 subjects (5 males, 7 females; aged 43–75 (mean ± SD, 63 ± 11) yr; BMI 23.0–56.0 (35.2 ± 9.0) kg/m2) completed in the study. By Vitros, the EGLs (mean [95% confidence interval] for CPB, 4.1 [2.0, 6.2] g (n = 11), and MCD, 3.8 [1.6, 6.0] g (n = 11) were similar to those by glucometer CPB, 5.8 [2.5, 9.1] g (n = 12) and MCD, 3.0 [0.8, 5.2] g (n = 11). There were no episodes of hypoglycemia during the study. Loose stools were experienced by 1 subject after CPB and by a different subject after MCS, events considered to be possibly related to the study products. ConclusionsThe results suggest it is feasible and safe to measure EGL in people with T2D not taking insulin. Funding SourcesSimply Good Foods USA, Inc.

Immune‐modulating Effects of Low Carbohydrate Ketogenic Foods in Healthy Canines

Ketogenic foods (KF) limit digestible carbohydrate but contain high fat, and have antioxidant and anti‐inflammatory effects as well as improving mitochondrial function. Beta hydroxybutyrate (BHB), one of the ketone bodies, has been reported to reduce pro‐inflammatory NLRP3 inflammasome, as well chemokines including CXCL2 and CCL5 in cultures. We assessed the immune‐modulating effects of two low carbohydrate (LoCHO) foods varying in protein and fat, and compared their effects to a food replete with carbohydrate (CON) in healthy canines. This study was approved by IACUC and the Animal Welfare Committee, Hills PNC. Macronutrients (P/F/C; % energy) for the foods were: CON (23/34/35), LoCHO1 (53/39/8) and LoCHO2 (27/67/5). We previously reported that LoCHO2 significantly increased circulating BHB compared to both CON and LoCHO1 although LoCHO1 did not increase BHB above that found when dogs consumed CON food. In the present study, dogs were fed control food (CON; ketogenic ratio (KR 0.46) followed by LoCHO1 (KR: 0.97), then LoCHO2 (KR: 1.63) or LoCHO2 followed by LoCHO1. Each food was fed for 5 weeks, with collections in the 5th week; 15 weeks feeding total. Gene expression for inflammatory cytokines and receptors from whole blood collected in PAXgene RNA tubes from 10 dogs was assessed using Canine RT2 Profiler PCR array. When compared to CON there was a significant decrease in several pro‐inflammatory cytokines/chemokines in both LoCHO1 and LoCHO2 groups including CCL1, CCL8, CCL13, CCL17,CCL24, CX3CL1, CXCR1, IL‐10RA, IL‐17A, IL‐1RN, IL‐5, IL‐9, and SPP1 (all p&lt;0.05). Interestingly, a subset of inflammatory proteins that decreased in LoCHO1, but not in LoCHO2, included IL‐33, IL‐6R, Il‐7, IL‐8, NAMPT, and TNFRSF11B. In contrast, the decrease in inflammatory markers in LoCHO2, but not in LoCHO1, included C5, CSF3, IFN‐γ, IL‐3, IL‐10RB, IL‐17C, TNFSF13, TNFSF13B, and TNFSF14. Decreased levels of selected cytokines indicate the ability of both low‐carbohydrate foods to exert an anti‐inflammatory effect and provide a strong rationale for testing its efficacy in dogs with inflammatory conditions. For instance, cytokines including IL‐7 and IL‐8 are increased in chronic inflammatory conditions such as colitis, dermatitis, as well as in autoimmune conditions. Increased IL‐33 is also associated with chronic lesional skin of atopic dermatitis in canines and these conditions might benefit from LoCHO1 food. LoCHO2 food decreased complement component 5 (C5) and blockade of C5 attenuates renal dysfunction including glomerulopathy. LoCHO2 also decreased TNFSF13, a member of the tumor necrosis factor superfamily, highly expressed in tumors, and associated with poor prognosis, indicating its potential as a nutritional intervention strategy for cancer. Taken together, our results indicate that both LoCHO1 and LoCHO2 foods might be important as part of immune‐modulating therapeutic nutritional strategies to reduce inflammation or inflammatory pathways to maintain health in canines.

Navike u ishrani i životnom stilu obolelih od dijabetesa Melitusa tipa 2 u Subotici

Introduction: The activities of the health care service in the control of diabetes and the improvement of glucoregulation of patients are primarily focused on lifestyle modification. The goal of this study was to review the recommendations in the field of adequate nutrition and lifestyle and to assess the health behavior of patients with type 2 diabetes in Subotica. Methodology: The research was conducted in the form of a cross-sectional study in February 2017 at the Diabetes Counseling Center of the Health Center in Subotica, Serbia. The study included 114 patients with type 2 diabetes. The research instrument consisted of a customized survey questionnaire. Results: Only a third of the patients actually consumed at least five meals a day, that was suggested as part of the treatment. Three-quarters of patients always prepared their own meals, but 41% of them never read the declarations about the ingredients of the food they consumed. Twenty percent of patients did not know how to assemble a healthy plate and what low-carbohydrate foods were. Two-thirds of patients used dietary supplements. A relatively small number, one-quarter of patients, consumed alcoholic beverages and 22 patients were active smokers. More than half of the patients exercised lightly, although 68% of them were dissatisfied with their body weight. Conclusion: We identified some deficiencies in the health behavior and lifestyle of people with diabetes. There is a recommendation to repeat the information on proper nutrition and the importance of physical activity in achieving ideal health and optimal glucoregulation during the educational work with patients.

Глюкозо-галактозная мальабсорбция: патогенез, диагностика, диетотерапия

Congenital glucose-galactose malabsorption is an orphan autosomal recessive life-threatening disease resulting from a selective defect in the intestinal glucose and galactose/sodium co-transport system in the brush border membrane of the enterocyte. In glucose-galactose malabsorption, glucose, galactose, as well as di- and polysaccharides: starch, dextrins, maltose, sucrose, lactose cannot be absorbed. The disease manifests itself in the neonatal period in the form of severe watery osmotic diarrhea, leading to rapid dehydration and metabolic acidosis. The only treatment is diet therapy with the exclusion of all carbohydrates except fructose. In infants with glucose-galactose malabsorption, diet therapy using specialized formulas with fructose as a carbohydrate component is vital. Subsequently, such infants need the introduction of low-carbohydrate complementary foods with primarily fructose content. The article presents a clinical example of the disease course and its successful dietary correction in an infant using a specialized formula and a specific diet. Key words: congenital diarrheal disorders, glucose-galactose malabsorption, infants, diet therapy, specialized milk formula based on fructose

Supplementary comparison study - fipronil-sulfone in chicken egg powder (APMP.QM-S16)

Main textThe APMP.QM-S16 comparison was coordinated by the National Institute of Metrology, China (NIM) on behalf of the Technical Committee on Amount of Substance (TCQM) of the Asia Pacific Metrology Programme (APMP) and the Organic Analysis Working Group (OAWG) of the Comité Consultatif pour la Quantité de Matière (CCQM) for National Measurement Institutes (NMIs) and Designated Institutes (DIs) which provide measurement services in organic analysis under the 'Comité International des Poids et Mesures' Mutual Recognition Arrangement (CIPM MRA).Fipronil is a broad-spectrum insecticide attributed to the phenylpyrazole family. Due to its high toxicity to invertebrates and long persistence, it has been one of the most widely used insecticides to control household and agricultural pests since its appearance in the market. Fipronil has also shown genotoxicity and cytotoxicity to many vertebrate animals, like mammals, birds, fish, reptiles and so on. Moreover, fipronil could have negative effects on the liver, kidneys and thyroid glands of humans. Its oxidation metabolite is fipronil-sulfone that could induce even higher toxicity than fipronil. In 2017, a fipronil-in-egg scandal occurred in the European Union (EU) that involved more than 15 states. The detected concentration of the sum of fipronil and fipronil-sulfone was many times higher than the EU maximum residue limit in bird egg (5 μg/kg). Besides European countries, fipronil-contaminated eggs were also found in Asian areas like China, Hong Kong and South Korea. Systematic monitoring and control of pesticide residues are necessary to safeguard public health.Fipronil-sulfone was selected to be representative of pesticide residues in this comparison. This study provides the means for assessing measurement capabilities for determination of low-polarity measurands in a procedure that requires extraction, clean-up, analytical separation, and selective detection in a food matrix. Generally, it provides a demonstration of a laboratory's capabilities in determining the medium molecular weight analytes (mass range 300-500) with low polarity (pKow < -2) at mass fraction levels of 4 to 400 μg/kg in a high fat, high protein and low carbohydrate food matrix that falls within Sector 4 of the AOAC Food-matrix Triangle.Seven NMIs/DIs participated in the APMP.QM-S16 comparison. Different methods such as liquid-liquid extraction, QuEChERS and SPE were applied in the sample pre-treatment and HPLC-MS/MS, HPLC-HRMS, and GC-MS/MS were applied for detection by the participants. Most participating laboratories applied isotope dilution mass spectrometry (IDMS) techniques with fipronil-sulfone-13C4,15N2 as internal standard, except EC-JRC-Geel which used matrix matched external calibration method. In the aspect of the traceability for the measurement results, most participants either used their own CRMs, other NMIs' CRMs or the pure materials assessed in-house to guarantee traceability to SI unit. One lab was excluded from the SCRV evaluation, as they did not meet the CIPM metrological traceability requirements. As a result, six sets of valid results were used for the estimation of the SCRV. Finally Hierarchical Bayes was considered to be the appropriate estimator to calculate SCRV, which was determined as 121.82 μg/kg with a standard uncertainty of 1.77 μg/kg. Inspection of the degree of equivalence plots for the fipronil-sulfone mass fraction assignments in APMP.QM-S16 indicated that there was excellent agreement of results.To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database https://www.bipm.org/kcdb/.The final report has been peer-reviewed and approved for publication by the CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

Making “gluten free” cauliflower (Brassica oleracea var. botrytis L.) flour to make tortillas with linaza (Linum usitatissimum) and chía (Salvia hispánica)

The current interest in the ketogenic diet provides the industry with the opportunity to develop new and better products, such as gluten-free foods for celiac patients and low-carbohydrate foods for overweight people, which in turn help prevent diseases that this provocative suffering. The tortilla is a globalized food in the daily diet, which makes it possible to add alternative ingredients. The objective of this work was to make cauliflower flour to make tortillas with flaxseed and chia with low carbohydrate content to verify if it is feasible as a daily food for those suffering from celiac disease, incorporate them into the ketogenic diet and in turn prevent the diseases caused by being overweight. For this purpose, tortillas with a diameter of 12 cm. and a thickness of 1 mm. were made with 50% cauliflower flour, 25% powdered chia and 25% powdered flaxseed.

CCQM-K146.1 subsequent comparison low-polarity analyte in high fat food: benzo[a]pyrene in olive oil

Main textThe Track A key comparison on the determination of benzo[a]pyrene in olive oil (CCQM-K146) organized by the CCQM was finished in 2020. This key comparison was related to low polarity analytes in a high fat, low protein, low carbohydrate food matrix and was the model system selected to align with the OAWG strategy for comparisons to underpin the food sector. Due to requests from the participants of this comparison, CCQM organized a supplementary comparison CCQM-K146.1 for those NMIs or DIs who did not participate in CCQM-K146 or wished to improve their results from CCQM-K146.5 NMIs and DIs participated in CCQM-K146.1. Different extraction methods and SPE cartridges were applied in the sample pre-treatment. HPLC-FLD, GC-MS/MS and GC-TOF MS were applied for detection by the participants. Certified reference materials (CRMs) were adopted or pure materials assessed in house to guarantee traceability to SI for the calibrants.NIM's result for CCQM-K146 was equivalent with the CCQM-K146 KCRV, thus they demonstrated acceptable performance in determining the measurand and their result for CCQM-K146.1 was used as the KCRV. The KCRV and u(KCRV) values were 8.69 μg/kg and 0.18 μg/kg, respectively. The random effects model obtained in CCQM-K146 suggests a dark uncertainty term (tau) is present, and the tau of CCQM-K146 normalized to CCQM-K146.1 measurand level is included in the estimation of degrees of equivalence (DoE) for the participants in CCQM-K146.1. This helps establish a consistent expectation of performance amongst the two studies, even when comparing to a different anchor KCRV.One participant demonstrated their capabilities for this analysis, whereas the other three were all deviated from the KCRV. This supplementary comparison directly illustrates the capabilities in determining mass fraction of organic compounds, with molecular mass of 100 g/mol to 500 g/mol, having low polarity pKow < -2, in mass fraction range from 0.1 μg/kg to 1000 μg/kg in a high fat, low protein, low carbohydrate food matrix.To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/.The final report has been peer-reviewed and approved for publication by the CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

Palatable Noodles as a Functional Staple Food Made Exclusively from Yellow Peas Suppressed Rapid Postprandial Glucose Increase.

Legumes are low-carbohydrate food and are abundant in dietary fiber. In order to provide a functional staple food that does not cause a rapid increase in postprandial blood glucose levels, four kinds of legumes were focused on as ingredients. Noodles made from dehulled yellow pea, unshelled yellow pea, chickpea, and lentil were prepared and evaluated as functional staple foods for controlling blood glucose via an in vitro digestion method. We also measured breaking stress and breaking strain using a creep meter, as well as sensory tests on a 9-point hedonic scale. The noodles made from yellow pea had high values for both breaking stress and breaking strain, and was highly regarded in the sensory tests. Therefore, the noodles made from yellow pea on postprandial glucose and insulin response were measured in a randomized double-blind study (n = 12). The results show that noodles made from yellow pea have a low glycemic index (50.4), and have potential as a functional staple food.

Effect of JumpstartMD, a Commercial Low-Calorie Low-Carbohydrate Physician-Supervised Weight Loss Program, on 22,407 Adults.

Background Commercial weight loss programs provide valuable consumer options for those desiring support. Several commercial programs are reported to produce ≥3-fold greater weight loss than self-directed dieting. The effectiveness of JumpstartMD, a commercial pay-as-you-go program that emphasizes a low-to-very-low-carbohydrate real-food diet and optional pharmacologic treatment without prepackaged meals or meal replacement, has not previously been described. Methods Completer and last observation carried forward (LOCF) of clinic-measured weight loss (kg) in 18,769 female and 3638 male JumpstartMD participants. Results Completers lost (mean ± SE) 8.7 ± 0.04 kg, 9.5 ± 0.04% with 44.5 ± 0.5% achieving ≥10% weight loss at 3 months (mo, N = 14,999 completers); 11.8 ± 0.1 kg, 12.6 ± 0.1% with 66.4 ± 0.6% achieving ≥10% weight loss at 6 mo (N = 11,805); and 11.5 ± 0.2 kg, 12.0 ± 0.2% with 57.6 ± 0.9% achieving ≥10% weight loss at 12 mo (N = 8514). LOCF estimates were −6.5 ± 0.03 kg, −7.2 ± 0.03% with 27.1 ± 0.3% achieving ≥10% weight loss at 3 mo; −7.7 ± 0.04 kg, −8.5 ± 0.04% with 36.3 ± 0.3% achieving ≥10% weight loss at 6 mo; and −7.7 ± 0.1 kg, −8.4 ± 0.1% with 34.6 ± 0.3% achieving ≥10% weight loss after 12 mo. Frequent health coach meetings was a major determinant of weight loss, with women and men attending ≥75% of their weekly appointments losing 8.8 ± 0.04 and 11.9 ± 0.1 kg, respectively, after 3 mo, 13.1 ± 0.1 and 16.5 ± 0.3 kg after 6 mo, and 16.5 ± 0.3 and 19.4 ± 0.8 kg after 12 mo. Phentermine and phendimetrazine had a minor effect in women only at 1 (6.1% greater weight loss than untreated), 2 (4.1%), and 3 mo (1.2%), but treated patients showed longer enrollment than nontreated during the first 3 (females: +0.4 ± 0.01; males: +0.3 ± 0.04 mo), 6 (females: +1.1 ± 0.04; males: +1.0 ± 0.1 mo), and 12 mo (females: +2.7 ± 0.1; males: +2.4 ± 0.2 mo). JumpstartMD produced generally greater weight loss than published reports for other real-food and prepackaged-meal commercial programs and somewhat greater or comparable losses to meal replacement diets. Conclusion A one-on-one medically supervised program that emphasized real low-carbohydrate foods produced effective weight loss, particularly in those attending ≥75% of their weekly appointments.

CCQM-K146 low-polarity analyte in high fat food: benzo[a]pyrene in olive oil

The demonstration of competency and equivalence for the assessment of levels of contaminants and nutrients in primary foodstuffs is a priority within the 10-year strategy for the CCQM Organic Analysis WG series of Track A core comparisons. The area of low polarity analytes in high fat foods had not been covered by the OAWG for many years since the key comparison CCQM-K21 p,p'-DDT in fish oil occurring in 2000.Benzo[a]pyrene (BaP) is one of the markers for the occurrence of PAHs in foods, for which maximum residue limits are enforced in many countries. Edible oil and fats are the main source of human PAH intake. BaP may form in edible oils by pyrolytic processes, such as incomplete combustion of organic substances. Worldwide regulatory limits of BaP in edible fats and oils are from 2.0 μg/kg to 10 μg/kg. Comparable and traceable measurement results for BaP in oil are important worldwide. Thus BaP in edible oil was the model system selected to align within the OAWG strategy.16 NMIs and DIs participated in CCQM-K146. Different methods such as liquid-liquid extraction, GPC and SPE were applied in the sample pre-treatment and HPLC-FLD, HPLC-MS/MS, and GC-MS or GC-MS/MS were applied for detection by the participants. Most participants either used their own CRMs, other NMI's CRMs or pure materials assessed in house to guarantee traceability to SI for the calibrants. One lab was excluded from the KCRV evaluation, as they did not meet the CIPM metrological traceability requirements for their calibrant. Regarding the data evaluation, five labs withdrew their results from the statistical evaluation of the KCRV for technical reasons. In line with the OAWG guidance document for KCRV estimator selection, the Hierarchical Bayes option was selected for the KCRV value, which was determined as 2.74 μg/kg with a standard uncertainty of 0.03 μg/kg. The remaining ten institutes that were included in the calculation of the consensus KCRV all agreed within their standard uncertainties.In general, the majority of CCQM-K146 participants performed very well. Therefore, this Track A Key Comparison directly illustrates the capabilities in determining mass fraction of organic compounds, with molecular mass of 100 g/mol to 500 g/mol, having low polarity pKow < -2, in mass fraction range from 0.1 μg/kg to 1000 μg/kg in a high fat, low protein, low carbohydrate food matrix.Main textTo reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/.The final report has been peer-reviewed and approved for publication by the CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).