Lower Assimilation Research Articles

Analysis of the Relative Effectiveness of Curcumin Loaded Chitosan Nanoparticles and Pure Curcumin Compound in the Treatment of Leishmaniasis.

Aim: A proficient drug conveyance plan is expected to treat cutaneous leishmaniasis (CL). Regardless of their deficient solvency, significant expense, low assimilation, and rising medication-safe Leishmania spp., pentavalent antimonials and curcumin (CC) may fix leishmaniasis. Drug conveyance frameworks (DDS) might be utilized to treat CL without these worries. Methodology: We tried free CC and Curcumin-stacked nanoparticles produced using chitosan (CC-CNPs) for antileishmanial movement in-vitro. Nanoparticles of Chitosan (CNPs) were produced by tripolyphosphate (TPP), which are negatively charged ionic gelation. CC was added to the CNPs mix. The size, surface shape, epitome adequacy (EE), drug stacking content (DLC), and surface charge of the NPs were characterized using various approaches. The MTT test was performed to evaluate their effectiveness against Leishmania tropica promastigotes and axenic amastigotes after characterization. Results: Round CC-CNPs with a typical molecule size of 276 nm, a zeta capability of (+18.74 mV), and 88% embodiment viability were integrated. Following 72 hours, free CC restrained promastigotes and axenic amastigotes parasite load by 64 and 70%, separately, though CC-CNPs repressed parasites by 91 and 86%. CC-CNPs diminished parasite suitability better than free CC. The half-maximal inhibitory focus (IC50) of CC-CNPs was a lot of lower than that of free CC. Conclusion: In a portion-time subordinate way, CC-CNPs showed more antileishmanial action than unbound CC. In-vivo testing is expected for CL nearby treatment with this detailing.

Microplastics in feed affect the toxicokinetics of persistent halogenated pollutants in Atlantic salmon

Microplastics (MPs) are carriers of persistent organic pollutants (POPs). The influence of MPs on the toxicokinetics of POPs was investigated in a feeding experiment on Atlantic salmon (Salmo salar), in which fish were fed similar contaminant concentrations in feed with contaminants sorbed to MPs (Cont. MPs); feed with virgin MPs and contaminated feed (1:1), and feed with contaminants without MPs (Cont.). The results showed that the salmon fillets accumulated more POPs when fed with a diet where contaminants were sorbed to the MPs, despite the 125–250 μm size MPs themselves passing the intestines without absorption. Furthermore, depuration was significantly slower for several contaminants in fish fed the diet with POPs sorbed to the MPs. Modelled elimination coefficients and assimilation efficiencies of lipophilic chlorinated and brominated contaminants correlated with contaminant hydrophobicity (log Kow) within the diets and halogen classes. The more lipophilic the contaminant was, the higher was the transfer from feed to salmon fillet. The assimilation efficiency for the diet without MPs was 50–71% compared to 54–89% for the contaminated MPs diet. In addition, MPs caused a greater proportional uptake of higher molecular weight brominated congeners. In the present study, higher assimilation efficiencies and a significantly higher slope of assimilation efficiencies vs log Kow were found for the Cont. MPs diet (p = 0.029), indicating a proportionally higher uptake of higher-brominated congeners compared to the Cont. diet. Multiple variance analyses of elimination coefficients and assimilation efficiencies showed highly significant differences between the three diets for the chlorinated (p = 2E-06; 6E-04) and brominated (p = 5E-04; 4E-03) congeners and within their congeners. The perfluorinated POPs showed low assimilation efficiencies of <12%, which can be explained by faster eliminations corresponding to half-lives of 11–39 days, as well as a lower proportional distribution to the fillet, compared to e.g. the liver.

Beyond singular perspectives: Minority-group members’ acculturation expectations and tightness

Previous research has established that ethnic minority groups experience specific acculturation expectations from the majority group. However, this research has largely ignored minority-group members’ expectations toward their own group and its heterogeneity. Sampling 327 Haredi Israeli minority-group members, the present study had three main objectives: (1) Identify the acculturation expectations individuals hold toward their minority group; (2) identify to which extent those expectations are uniform (i.e., tight) or differ (i.e., loose) toward subgroups of their overarching group, operationalized as within-individual standard deviations; and (3) examine whether a set of psychological variables is meaningfully correlated with both acculturation expectation means and tightness. Although participants on average endorsed separation expectations, these expectations were also relatively loose (i.e., not uniformly expected from all Haredi subgroups). Whereas integration expectations were also relatively pronounced and loose, assimilation and marginalization expectations were low and tight. Perceived physical threat toward the group was associated with high assimilation expectations. Personal mortality thereat was associated with higher integration expectations, whereas past victimization was associated with lower assimilation and higher separation expectations. The belief in the eternity of the group was positively associated with separation expectations and negatively associated with marginalization, integration, and assimilation expectations. The belief in the eternity of the group was also associated with looser separation expectations and tighter assimilation expectations. Bi-cultural identity harmony was associated with higher assimilation and integration expectations, lower separation expectations, and generally more loose expectations. We discuss the implications of the findings for research and society.

CIPK15-mediated inhibition of NH4+ transport protects Arabidopsis from submergence

Ammonium (NH4+) serves as a vital nitrogen source for plants, but it can turn toxic when it accumulates in excessive amounts. Toxicity is aggravated under hypoxic/anaerobic conditions, e.g., during flooding or submergence, due to a lower assimilation capacity. AMT1; 1 mediates NH4+ uptake into roots. Under conditions of oxygen-deficiency, i.e., submergence, the CBL-interacting protein kinase OsCIPK15 has been shown to trigger SnRK1A signaling, promoting starch mobilization, thereby the increasing availability of ATP, reduction equivalents and acceptors for NH4+ assimilation in rice. Our previous study in Arabidopsis demonstrates that AtCIPK15 phosphorylates AMT1; 1 whose activity is under allosteric feedback control by phosphorylation of T460 in the cytosolic C-terminus. Here we show that submergence cause higher NH4+ accumulation in wild-type, plant but not of nitrate, nor in a quadruple amt knock-out mutant. In addition, submergence triggers rapid accumulation of AtAMT1;1 and AtCIPK15 transcripts as well as AMT1 phosphorylation. Significantly, cipk15 knock-out mutants do not exhibit an increase in AMT1 phosphorylation; however, they do display heightened sensitivity to submergence. These findings suggest that CIPK15 suppresses AMT activity, resulting in decreased NH4+ accumulation during submergence, a period when NH4+ assimilation capacity may be impaired.

Back to the 'roots' of research: a hypothesis-driven approach provides predictive understanding of plant-herbivore-microbe interactions.

This article is a Commentary on Böttner et al. (2023), 239: 1475–1489.

Beer Brewed with Sake Yeast Strain Has Unique Sake-like Flavors

"Sake Yeast Beer” is a beer style characterized by the unique flavor of the sake yeast strain; however, it cannot ferment wort because of the low assimilation ability of maltose. Herein, a sake yeast strain was bred for improved maltose assimilation ability and an original sake yeast strain optimized for a beer brewing environment was developed. The mutant sake yeast strains produced large amounts of isoamyl acetate (3-methylbutyl acetate) and ethyl caproate (ethyl hexanoate) responsible for the ginjo-aroma of sake. A detailed analysis of the volatile components in a small-scale beer brewing test revealed that the beer brewed with BMK3, one of the mutant strains, was composed of many volatile components generated by yeast, such as esters, alcohols, and fatty acids, in comparison to the beer fermented with a general brewer’s yeast. Moreover, in a full-scale beer brewing test using BMK3, the beer was composed of isoamyl acetate and ethyl caproate at 2.3 and 6.7 ppm, respectively. On the sensory evaluation of two beers fermented with BMK3 and a general brewer’s yeast, the beer brewed with BMK3 had the unique characteristics of “ginjo-aroma like fruit,” remaining “sweetness,” and a “rich body but crisp finish.”

METHODOLOGY FOR WORKING WITH CHILDREN WITH LOW ASSIMILATION IN MUSIC CULTURE CLASSES OF SECONDARY SCHOOLS

METHODOLOGY FOR WORKING WITH CHILDREN WITH LOW ASSIMILATION IN MUSIC CULTURE CLASSES OF SECONDARY SCHOOLS

LDPE and biodegradable PLA-PBAT plastics differentially affect plant-soil nitrogen partitioning and dynamics in a Hordeum vulgare mesocosm

Micro and macroplastics are emerging contaminants in agricultural settings, yet their impact on nitrogen (N) cycling and partitioning in plant-soil-microbial systems is poorly understood. In this mesocosm-scale study, spring barley (Hordeum vulgare L.) was exposed to macro or microplastic produced from low density polyethylene (LDPE) or biodegradable plastic at concentrations equivalent to 1, 10 and 20 years of plastic mulch film use. Partitioning of 15N-labelled fertiliser into plant biomass, soil and leachate yielded a partial mass balance. Soil N partitioning was probed via compound-specific 15N-stable isotope analyses of soil microbial protein. Concentration-dependent decreases in plant 15N uptake occurred with increased leached nitrogen for LDPE microplastic. Assimilation into soil microbial protein was higher for biodegradable plastics, which we associate with early-stage biodegradable plastic degradation. Partitioning of 15N into inorganic soil N pools was affected by LDPE size, with lower assimilation into the microbial protein pool. While microplastics and macroplastics altered soil N cycling, the limited impacts on plant health indicated the threshold for negative effects was not reached at agriculturally relevant concentrations. This study highlights the difference between conventional and biodegradable plastics, and emphasises that the interplay of micro and macroplastics on soil N cycling must be considered in future studies.

Divergence in digestive and metabolic strategies matches habitat differentiation in juvenile salmonids.

Divergent energy acquisition and processing strategies associated with using different microhabitats may allow phenotypes to specialize and coexist at small spatial scales. To understand how ecological specialization affects differentiation in energy acquisition and processing strategies, we examined relationships among digestive physiology, growth, and energetics by performing captive experiments on juveniles of wild coho salmon (Oncorhynchus kisutch) and steelhead trout (O. mykiss) that exploit adjacent habitats along natural low‐to‐high energy flux gradients (i.e., pools versus riffles) in coastal streams. We predicted that: (i) the specialization of steelhead trout to high‐velocity, high‐energy habitats would result in elevated food intake and growth at the cost of lower growth efficiency relative to coho salmon; (ii) the two species would differentiate along a rate‐maximizing (steelhead trout) versus efficiency‐maximizing (coho salmon) axis of digestive strategies matching their ecological lifestyle; and (iii) the higher postprandial metabolic demand (i.e., specific dynamic action, SDA) associated with elevated food intake would occupy a greater fraction of the steelhead trout aerobic budget. Relative to coho salmon, steelhead trout presented a pattern of faster growth and higher food intake but lower growth efficiency, supporting the existence of a major growth versus growth efficiency trade‐off between species. After accounting for differences in ration size between species, steelhead trout also presented higher SDA than coho salmon, but similar intestinal transit time and lower assimilation efficiency. Both species presented similar aerobic budgets since the elevated SDA of steelhead trout was largely compensated by their higher aerobic scope relative to coho salmon. Our results illustrate the key contribution of digestive physiology to the adaptive differentiation of juvenile growth, energetics, and overall performance of taxa with divergent habitat specializations along a natural productivity gradient.

Patterns in climate and seedling establishment at a dry tropical treeline

In some internal valleys of the northern Andes, precipitation at treeline elevation is seasonal, with one or two dry periods that could exert a strong control on tree seedling survival. However, little is known about (1) the severity of the dry periods, due to a lack of climate data, or (2) the effect of drought on tree seedling demographic dynamics and how this is modulated by local plant interactions in these tropical-alpine ecosystems. We studied annual precipitation variation and seedling demography and ecophysiology in relation to microclimate and neighboring plants in a dry treeline ecotone at 3100 m a.s.l in the Venezuelan Andes. We selected seedlings of the dominant tree species Cybianthus marginatus and Clusia multiflora at three sites, each with three adjacent 10 × 10-m plots within the forest, forest border, and páramo. For both species, seedling density was zero in the páramo and did not differ between border and forest. Growth rates were low for both species, but higher for Cybianthus in the border than in the forest. Survival rates were high in both environments. Low assimilation and transpiration rates were found in both species regardless of the environment. The spatial association of seedlings with moisture-regulating elements such as mosses, ferns and basal rosettes may indicate a benefit of better hydric conditions in their neighborhood. Our study shows a strong limitation for tree establishment in the páramo, but high rates of survival at the edge of the forest. This suggests a scenario of slow potential forest expansion at this tropical treeline, tree cover advancing in a closed front by the successive formation of forest-edge vegetation allowing tree seedling establishment outside of the forest.

Sightseeing spot satisfaction of inbound tourists: comparative analysis of first-time visitors and repeat visitors in Japan

PurposeThis study aims to enhance knowledge on marketing strategies to increase repeat visitors. Furthermore, the authors suggest using appropriate destination information tailored to first-time visitors and repeat visitors as social capital.Design/methodology/approachThis study compares the differences in satisfaction between first-time foreign tourists and those repeat visiting. The authors apply a theoretical framework based on optimal distinctiveness theory (ODT) and consumer knowledge to determine which actions maximize the satisfaction of each group. Then, the relationships among assimilation desire, differentiation desire and satisfaction are quantitatively analyzed.FindingsThe results show the difference in the relationship between assimilation and travel satisfaction for first-time visitors and repeat visitors. First-time visitors are satisfied with popular sightseeing spots with higher assimilation level, whereas repeat visitors are satisfied with moderately unpopular sightseeing spots with lower assimilation level. The results clarify that information reflecting only the popularity of destinations is significantly effective for first-time visitors, but unsuitable for repeat visitors. Therefore, it would be possible to propose to repeat visitors a combination of “moderately differentiated” destinations.Originality/valueThe first contribution is that on the basis of Brewer (1991), the quantitative analysis confirmed that the social identity of an individual changes from assimilation to differentiation with the accumulation of experience until the optimal point is found. The second contribution is that we combined several fields such as ODT (Brewer, 1991), familiarity (Alba and Hutchinson, 1987), trial/repeat behavior (Iyengar et al., 2015) and reference groups (Peter & Olson, 2010). The third contribution is that the authors proposed marketing strategies on the basis of the empirical analysis to increase the number of inbound tourists.

The relationships between maize (Zea mays L.) lodging resistance and yield formation depend on dry matter allocation to ear and stem

Lodging is a critical constraint to yield increase. There appear to be tradeoffs between yield formation and lodging resistance in maize. Hypothetically, it is feasible to reduce lodging risk as well as increase grain yield by optimizing dry-matter allocation to different organs under different environments. A three-year field experiment was conducted using four maize cultivars with differing lodging resistances and five growing environments in 2018–2020. Lodging-susceptible (LS) cultivars on average yielded more than lodging-resistant (LR) cultivars when lodging was not present. The yield components kernel number per ear (KN) and thousand-kernel weight (TKW) were both negatively correlated with lodging resistance traits (stalk bending strength, rind penetration strength, and dry matter weight per internode length). Before silking, the LR cultivar Lishou 1 (LS1) transported more assimilates to the basal stem, resulting in a thicker basal stem, which reduced dry matter allocation to the ear and in turn KN. The lower KN of LS1 was also due partly to the lower plant height (PH), which increased lodging resistance but limited plant dry matter production. In contrast, the LS cultivars Xianyu 335 (XY335) and Xundan 20 (XD20) produced and allocated more photoassimilates to ears, but limited dry matter allocation to stems. After silking, LS cultivars showed higher TKW than LR cultivars as a function of high photoassimilate productivity and high assimilate allocation to the ear. The higher lodging resistance of LS1 was due mainly to the greater assimilate allocation to stem after silking and lower PH and ear height (EH). High-yielding and high-LR traits of Fumin (FM985) were related to optimized EH and stem anatomical structure, higher leaf productivity, low assimilate demand for kernel formation, and assimilate partitioning to ear. A high pre-silking temperature accelerated stem extension but reduced stem dry matter accumulation and basal stem strength. Post-silking temperature influences lodging resistance and yield more than other environmental factors. These results will be useful in understanding the tradeoffs between KN, KW, and LR in maize and environmental influences on these tradeoffs.

Carbon Regulating Services of A 30-year-old Reclaimed Limestone Mine Area in Foothills of Himalaya

The study aims to assess various carbon regulating services of a limestone mine site, reclaimed 30 years back, compared to its adjoining natural forest. The natural forest is dominated by broad leaf species ( Quercus leucotricophora ) whereas, the reclaimed sites have Cupressus torulosa as the dominant species. Though there was no significant difference in the number of species of various life forms (trees, shrubs, herbs and grasses), total basal cover (TBC) estimated for the natural forest (2513.36cm 2 /100m 2 ) was much higher compared to the reclaimed site (584.30 to 997.07 cm 2 /100m 2 ). The difference is much higher in TBC of trees compared to shrubs. Annual litter production was also higher in the natural forest. Microbial activity in the top soil layer was found to have increased substantially in the reclaimed mine site. Soil respiration rate and soil microbial biomass in the reclaimed mine site was on a par with that of natural forest. It indicates a significant level of organic matter and detritus availability in the reclaimed sites' top soils since the soil's degradable organic carbon is the main fuel responsible for the CO emission during soil 2 respiration. Though the carbon assimilation rate recorded in the vegetation of the reclaimed area is on a par with that of vegetation of natural forest at the species level, at the community level, the reclaimed site may have lower assimilation owing to lower TBC. Therefore, the difference in vegetation structure and composition in the reclaimed site compared to the natural forest might significantly impact the overall carbon regulating services in the study area.

BERBERINE: A POTENT ADJUVANT IN PSORIASIS NANOTECHNOLOGY

Psoriasis is a multi-factorial disease represented by complex atomic scenes and cellular pathways that lead to abnormal cell development. The normal mixture has a clear target and limited cytotoxicity; in this sense, it can support the further development of new therapies for the treatment of this flexible disease. Berberine is an individual of the original berberine alkaloid family. It mainly exists in the roots, trunks and barks of various trees and has anti-psoriatic activity. In any case, according to the berberine organization, limited bioavailability and low assimilation rate are the two main obstacles, because only 0.5% of the ingested berberine is consumed in the small digestive tract, and this rate is when it enters the intestines. It is further reduced to 0.35%. Nano-based details are seen as the best way to increase the assimilation rate because substances accumulated at the nano-level can be quickly absorbed in the intestine. Useful methods based on nanotechnology have been developed to solve these problems to ultimately achieve greater sufficiency in the treatment of various diseases. The investigation introduced and essentially examined the anti-proliferative effects of berberine and nanotechnology-based repair technology for nano-scale transportation of berberine. Finally, we will thoroughly analyze and examine the current methods and promising viewpoints of the recent transportation of this alkaloid.

Effect of assimilate competition during early seed development on the pod and seed growth traits in soybean.

Although the seed remains small in size during the initial stage of seed development (the lag phase), several studies indicate that environment and assimilate supply level manipulations during the lag phase affect the final seed size. However, the manipulations were not only at the lag phase, making it difficult to understand the specific role of the lag phase in final seed size determination. It also remained unclear whether environmental cues are sensed by plants and regulate seed development or if it is simply the assimilate supply level, changed by the environment, that affects the subsequent seed development. We investigated soybean (Glycine max L. Merr.) seed phenotypes grown in a greenhouse using different source-sink manipulations (shading and removal of flowers and pods) during the lag phase. We show that assimilate supply is the key factor controlling flower and pod abortion and that the assimilate supply during the lag phase affects the subsequent potential seed growth rate during the seed filling phase. In response to low assimilate supply, plants adjust flower/pod abortion and lag phase duration to supply the minimum assimilate per pod/seed. Our results provide insight into the mechanisms whereby the lag phase is crucial for seed development and final seed size potential, essential parameters that determine yield.

Replacing concentrates with a high-quality hay in the starter feed of dairy calves: II. Effects on the development of chewing and gut fermentation, and selected systemic health variables

Early development of the rumen, rumination, and fermentation is highly important in dairy calves. Yet, common rearing practices with feeding of concentrate-rich starters may jeopardize them because of lacking physically effective fiber (peNDF). The main objective of this study was to establish the influence of the composition of the calf starter feed (only forage with 2 different qualities or concentrate-rich starter diet) on chewing behavior, rumen development, rumen and hindgut fermentation, and selected systemic health and stress variables of dairy calves. The experiment was carried out with 40 newborn Holstein-Friesian calves, randomly assigned to 4 different solid feed treatments: MQH = 100% medium-quality hay (9.4 MJ metabolizable energy, 149 g of crude protein, and 522 g of neutral detergent fiber/kg of dry matter); HQH = 100% high-quality hay (11.2 MJ of metabolizable energy, 210 g of crude protein, 455 g of neutral detergent fiber/kg of dry matter); MQH+C = 30% MQH + 70% starter concentrate; HQH+C = 30% HQH + 70% starter concentrate). All calves were up to 14 wk in the trial and received acidified whole milk ad libitum in the first 4 wk of life, thereafter in reduced quantity until weaning on 12 wk of age. Water and the solid feed treatments were available ad libitum throughout the trial. Chewing activity was measured in wk 4, 6, 10, and 12 using RumiWatch halters. Until wk 3, rumen fluid, feces and blood were sampled weekly, thereafter every 2 wk. Rumen mucosal thickness (RMT) was measured on the same days with rumen fluid samples. Data showed that calves fed the HQH diet consumed more peNDF and this was associated with longer rumination time (591 min/d) and more ruminating boli (709 boli/d) than calves fed concentrate-rich diets (MQH+C: 430 min/d, 518 boli/d; HQH+C: 430 min/d, 541 boli/d), whereas the MQH group was intermediate (539 min/d, 644 boli/d). Ruminal and fecal pH were higher in calves fed only hay (especially MQH) compared with calves with concentrate supplementation. In both hay-fed groups, ruminal and fecal short-chain fatty acids were shifted toward acetate, whereas only the HQH diet increased the butyrate proportion in the ruminal short-chain fatty acids profile. Ruminal ammonia concentration was at a high level only in the first 3 wk and decreased thereafter. Feeding HQH tended to increase ruminal ammonia, likely because of its high crude protein content and ruminal degradability as well as lower assimilation from rumen microbes. The RMT similarly, though nonlinearly, increased in all groups over the course of the experiment. When using RMT as an indicator of rumen development in dairy calves in the practice, our data suggest an RMT of 1.7mm and >2mm at wk 5 and 10 of life, respectively. Feeding did not affect the blood levels of aspartate aminotransferase, gamma glutamyl transferase, glutamate dehydrogenase, and cortisol. In conclusion, feeding high-quality hay, instead of concentrate-rich starter feeds, resulted in improved rumination and ruminal fermentation profile, without affecting ruminal pH and systemic and stress health variables.

Comparing life history traits and tolerance to changing environments of two oyster species (Ostrea edulis and Crassostrea gigas) through Dynamic Energy Budget theory.

To predict the response of the European flat oyster (Ostrea edulis) and Pacific cupped oyster (Crassostrea gigas/Magallana gigas) populations to environmental changes, it is key to understand their life history traits. The Dynamic Energy Budget (DEB) theory is a mechanistic framework that enables the quantification of the bioenergetics of development, growth and reproduction from fertilization to death across different life stages. This study estimates the DEB parameters for the European flat oyster, based on a comprehensive dataset, while DEB parameters for the Pacific cupped oyster were extracted from the literature. The DEB parameters for both species were validated using growth rates from laboratory experiments at several constant temperatures and food levels as well as with collected aquaculture data from the Limfjorden, Denmark, and the German Bight. DEB parameters and the Arrhenius temperature parameters were compared to get insight in the life history traits of both species. It is expected that increasing water temperatures due to climate change will be beneficial for both species. Lower assimilation rates and high energy allocation to soma explain O. edulis' slow growth and low reproductive output. Crassostrea gigas' high assimilation rate, low investment in soma and extremely low reserve mobility explains the species' fast growth, high tolerance to starvation and high reproductive output. Hence, the reproductive strategies of both species are considerably different. Flat oysters are especially susceptible to unfavourable environmental conditions during the brooding period, while Pacific oysters' large investment in reproduction make it well adapted to highly diverse environments. Based on the life history traits, aquaculture and restoration of O. edulis should be executed in environments with suitable and stable conditions.

A multifaceted assessment of the effects of polyethylene microplastics on juvenile gilthead seabreams (Sparus aurata).

Plastic pollution has become a major environmental and societal concern in the last decade. From larger debris to microplastics (MP), this pollution is ubiquitous and particularly affects aquatic ecosystems. MP can be directly or inadvertently ingested by organisms, transferred along the trophic chain, and sometimes translocated into tissues. However, the impacts of such MP exposure on organisms' biological functions are yet to be fully understood. Here, we used a multi-diagnostic approach at multiple levels of biological organization (from atoms to organisms) to determine how MP affect the biology of a marine fish, the gilthead seabream, Sparus aurata. We exposed juvenile seabreams for 35 days to spherical 10-20µm polyethylene primary MP through food (Artemia salina pre-exposed to MP) at a concentration of 5±1µg of MP per gram of fish per day. MP-exposed fish experienced higher mortality, increased abundance of several brain and liver primary metabolites, hepatic and intestinal histological defects, higher assimilation of an essential element (Zn), and lower assimilation of a non-essential element (Ag). In contrast, growth and muscle C/N isotopic profiles were similar between control and MP-exposed fish, while variable patterns were observed for the intestinal microbiome. This comprehensive analysis of biological responses to MP exposure reveals how MP ingestion can cause negligible to profound effects in a fish species and contributes towards a better understanding of the causal mechanisms of its toxicity.

Filter Feeding and Carbon and Nitrogen Assimilation of a Freshwater Bivalve (Unio douglasiae) on a Toxic Cyanobacterium (Microcystis aeruginosa)

We investigated the possible intake of toxic cyanobacteria (Microcystis aeruginosa) as a nutrient resource for a filter-feeder bivalve (Unio douglasiae) based on the measurement of feeding and assimilation rates of carbon and nitrogen in a limited space with no current for 72 h using 13C and 15N dual isotope tracers. With high clearance rates, the unionid rapidly removed Microcystis cells within 24 h, but only a small amount of carbon and nitrogen were incorporated into the tissues. Even with the low assimilation rates, the mussels showed more favorable uptake of carbon than of nitrogen from toxic Microcystis water, and of tissues, the gills and gut accumulated more carbon and nitrogen than the muscle and mantle. Collectively, our findings indicate that although Unio douglasiae effectively uptake toxic Microcystis cells, they can assimilate only low amounts of nutrients into tissues within three days, despite a non-flowing system.

Putting Sense and Mind into Your Enterprise Systems: A Qualitative Study of IS Assimilation in Large Public Organizations in India

The purpose of this paper is to investigate the information systems (IS) assimilation level of an enterprise system in the post-implementation phase, through the lens of IS governance mechanisms and IS support structures, impacted by the socio-cognitive processes. The research follows a qualitative approach and builds on semi-structured interviews with enterprise system stakeholders in large public sector organizations in India. The study posits that high levels of IS governance mechanisms and high levels of IS support structures lead to a high level of IS assimilation only in the presence of higher level socio-cognitive processes. A not-so-higher level of socio-cognitive fabric results in low or moderate IS assimilation levels in spite of high levels of IS governance and/or IS support structures. Despite close to a couple of decades of IS research on enterprise systems, IS assimilation is still an enigma for practitioners and academicians. The generalizability of the results of this study may be applicable to any public organization in a developing country, like India, which are using enterprise system solutions but are yet to reap the potential benefits. The results present a way forward for practitioners to ensure optimal resources and focus for the triad- IS governance, IS support structures and socio-cognitive processes.