Lotus Rhizomes Research Articles

The peels of sacred Lotus (Nelumbo nucifera) rhizomes suppress allergic reactions by inhibiting the A23187-induced degranulation in rat basophilic leukemia (RBL[sbnd]2H3) cells

We isolated ten compounds from methanolic extract of the peels of sacred lotus (Nelumbo nucifera) rhizomes which were identified as β-sitosterol linoleate 1, β-sitosterol 2, lupeol 3, stigmasterol 3-O-β-D-glucoside 4, oleanolic acid 5, betulinic acid 6, pinoresinol 7, 4-hydroxybenzoic acid 8, catechin 9 and gallocatechin 10. All of the isolated compounds from the peels of sacred lotus rhizomes are reported for the first time, and were investigated for their anti-allergic activity. We found that three of them, stigmasterol 3-O-β-D-glucoside 4, oleanolic acid 5 and pinoresinol 7, were capable of inhibiting A23187-induced degranulation in RBL-2H3 cells with IC50 values 0.18 ± 0.01 mM, 0.28 ± 0.06 mM, and 0.27 ± 0.01 mM, respectively. With an exception to 4, compounds 5 and 7 achieved the anti-allergic effect without affecting the cells viability even at higher concentrations with their selectivity indices (SI) being >5. By reducing A23187-induced degranulation, it is suggestive of a mechanism attenuation of Ca2+ elevation. Our findings suggest that, the peels of sacred lotus rhizomes would be beneficial for providing an inexpensive source for the production of bioactive compounds with anti-allergic effect.

Identifying dominant spoilage fungal species and preservative treatments for extending shelf life of whole lotus rhizomes at 4 °C

A preservative for whole lotus rhizome preservation was developed under 4 °C storage in this study. The study included the following steps: Firstly, isolation and identification of spoilage causing fungi of lotus rhizome, and four main spoilage causing fungi were identified, including Penicillium solitum, Geotrichum silvicola, Phytopythium helicoides, and Pseudeurotium zonatum. Secondly, selection of antifungal agent with good inhibitory ability against the above four spoilage causing fungi, finally 0.5 % potassium sorbate was selected considering edible security at the same time. Thirdly, the selection of suitable color fixative according to the indexes of root skin and flesh color differences, and sensory evaluation. The final color fixative composition: EDTA-2Na (8 g/L), ascorbic acid (8 g/L), and d-isoascorbic acid sodium (6 g/L). Finally, the antifungal agent and color fixatives were mixed to make the preservative. Soaked in preservative for 20min, drained and packed into PE bags, vacuumed and stored at 4 °C, the lotus rhizomes could maintain better color, got high sensory score, soluble solids content, and total acid content, and the microbial counts were always lower than the untreated group. The preservative could extend the shelf life of whole lotus rhizome to about 50 d at 4 °C.

Study on the formation mechanism of blackening in damaged lotus rhizome epidermis: Effects of polyphenols and iron.

Lotus rhizome is an important aquatic vegetable, but the blackening of lotus rhizome epidermis (LRE) seriously affects its appearance and quality, which makes lotus rhizome products unmarketable. In this study, the effects of polyphenols and iron on the LRE color were studied to explore the possible mechanism of LRE blackening. Results indicated that the measurable total phenols contents in the mud treatment (MT) group were significantly reduced, and the total iron contents were significantly increased compared with the bruised treatment group (p<0.05). The high-performance liquid chromatography results showed that the main polyphenols in LRE were dopa, gallocatechin, and catechin, as well as a small amount of catechol, epicatechin, proanthocyanidin B2, and proanthocyanidin C1. Moreover, the results of color difference and ultraviolet adsorption spectroscopy showed that there were obviously black or brown-gray of dopa (525nm), gallocatechin (504.5nm), and catechin (550 and 504.5nm) with FeCl2. The simulated system treatment of LRE further confirmed that the chromaticity effect of dopa and iron in bruised LRE was similar to that of the MT group, whereas 1% (w/w) ascorbic acid, 2% (w/w) EDTA-2Na, or 3% (w/w) citric acid could solely prohibit the blackening. This suggested that the dopa in LRE and FeCl2 in mud may mainly combine into [2(DOPA-2H+)+Fe3+]- through non-covalent interaction, which leads to the blackening of bruised LRE under neutral conditions. These results can guide the storage of lotus rhizomes and improve the development of the lotus rhizome industry.

Insight into the mechanisms of acid treatment on alkali-soluble polysaccharides from crispy and mealy lotus rhizomes

The present study investigated the structural disparities in cell wall polysaccharides between lotus rhizomes with mealy and crispy textures through a combination of acid and enzyme treatments. The results from size exclusion chromatography equipped with multi-angle light scattering detectors, viscometer detector and differential refractive index (SEC-MALS-DRI-VD) indicated that, before and after acid treatment, the CASP (alkali-soluble polysaccharides from crisp lotus rhizomes) were all semi-flexible chains using, while MASP (alkali-soluble polysaccharides from mealy lotus rhizomes) changed from flexible chains to semi-flexible. The atomic force microscope (AFM) images revealed a reduction in pectin chain length and decreased chain height of CASP following acid hydrolysis. However, MASP exhibited adhesion of pectin chains and formation of cluster structure. Through the analysis of monosaccharide composition result, the structural disparities between pectin are closely linked to the acid hydrolysis discrepancies observed in CASP and MASP. This study offers a molecular-level rationale for enhancing the thermal texture of fruits and vegetables through acid treatment.

NnNF-YB1 induced by the potassium fertilizer enhances starch synthesis in rhizomes of Nelumbo nucifera

Lotus (Nelumbo nucifera Gaertn.) is one of the most important aquatic vegetables in China, which is rich in starch and is important sources of energy for livings. Potassium fertilizer has important effects on physiological characteristics, yield and quality of lotus rhizomes. However, the relationship between starch and potassium fertilizer remains unclear. Here, we investigated the relationship between starch quality and potassium fertilizer. Compared with the treatment without potassium fertilizer (CK), the total starch content, amylose content, and amylopectin content of potassium fertilizer treatment 30 kg/667 m2 (K30) were significantly increased. RNA-seq showed that K30 induces more than 2000 differentially expressed genes (DEGs), which mainly enriched in biosynthesis of starch and sucrose metabolism, secondary metabolites, photosynthesis. Meanwhile, K30 significantly promoted the expression of most of key genes and transcription factors involved in the regulation of starch synthesis. We identified a NF-YB transcription factor NnNF-YB1, induced by potassium fertilizer could activate the expression of NnGBSSII by directly bind to its promoter. Transient overexpression of NnNF-YB1 resulted in the increase of total starch, amylose and amylopectin content in rhizomes of lotus. NnNF-YB1 positively regulated the expression of NnGBSSII and plays an important role in starch synthesis under potassium fertilizer treatment. Collectively, our study provides a reference for fertilizer application in lotus production. At the same time, we revealed a new mechanism of potassium fertilizer increasing starch content and improving the quality of starch in rhizomes.

Effects of Slow-Release Fertilizer on Lotus Rhizome Yield and Starch Quality under Different Fertilization Periods

Slow-release fertilizer is an environmentally friendly fertilizer that is widely used in crop cultivation instead of traditional nitrogen fertilizer. However, the optimal application time of slow-release fertilizer and its effect on starch accumulation and rhizome quality of lotus remains unclear. In this study, two slow-release fertilizer applications (sulfur-coated compound fertilizer, SCU, and resin-coated urea, RCU) were fertilized under three fertilization periods (the erect leaf stage, SCU1 and RCU1; the erect leaf completely covering the water stage, SCU2 and RCU2; and the swelling stage of lotus rhizomes, SCU3 and RCU3) to study the effects of different application periods. Compared with CK (0 kg∙ha-1 nitrogen fertilizer), leaf relative chlorophyll content (SPAD) and net photosynthetic rate (Pn) remained at higher levels under SCU1 and RCU1. Further studies showed that SCU1 and RCU1 increased yield, amylose content, amylopectin and total starch, and the number of starch particles in lotus, and also significantly reduced peak viscosity, final viscosity and setback viscosity of lotus rhizome starch. To account for these changes, we measured the activity of key enzymes in starch synthesis and the relative expression levels of related genes. Through analysis, we found that these parameters increased significantly under SCU and RCU treatment, especially under SCU1 and RCU1 treatment. The results of this study showed that the one-time application at the erect leaf stage (SCU1 and RCU1) could improve the physicochemical properties of starch by regulating the key enzymes and related genes of starch synthesis, thus improving the nutritional quality of lotus rhizome. These results provide a technical choice for the one-time application of slow-release fertilizer in lotus rhizome production and cultivation.

Effects of diet shift on the gut microbiota of the critically endangered Siberian Crane

Wetlands worldwide have suffered from serious degradation and transformation, leading to waterbirds increasingly dependent on agricultural fields for feeding. Although gut microbiota is an essential component of host health, the impacts of agricultural feeding on gut microbial community and pathogen transmission remain poorly understood. To fill this knowledge gap, we used 16S rRNA sequencing to characterize the fecal bacterial community of the Siberian Crane (Grusleucogeranus), a Critically Endangered species, that recently has shifted its foraging from largely Vallisneria tubers in Poyang Lake natural wetlands to crops (i.e., rice seeds and lotus rhizomes) in agricultural fields. We compared the bacterial communities between tuber foraging cranes and crop foraging cranes. Our results indicate that diet shift greatly modified the gut microbiota diversity, composition and function. Crop foraging cranes had higher microbiota diversity than tuber foraging cranes. The alteration in microbiota composition and function were correlated with change in food nutrition. Tuber (i.e., high in fiber) foraging cranes were enriched in Clostridiaceae with fiber digestion ability, and crop (i.e., high in carbohydrate) foraging cranes were enriched in bacterial taxa and functions related to carbohydrate metabolism. The flexibility of gut microbiota might enhance Siberian Cranes’ ability to adapt to novel diet and environment. However, many enriched families in crop foraging cranes were pathogenic bacteria, which might increase the susceptibility of cranes to pathogenic infection. Special caution should be taken to agricultural feeding waterbirds in Asia, where the widespread poultry-keeping in over-harvested rice fields might increase the transmission probability of pathogenetic bacteria among wild birds, domestic poultry and humans.

Nontarget Metabolites of Rhizomes of Edible Sacred Lotus Provide New Insights into Rhizome Browning

The “edible rhizome” variant of Nelumbo nucifera with various cultivars has a long history of use as a food in East Asia. In this study, 48 target metabolites were untargeted and identified in 212 rhizome cultivars (tropical and temperate types) using ultraperformance liquid chromatography-electrospray ionization quadrupole time-of-flight high-resolution mass spectrometry; among these, 32 compounds were newly reported in the rhizome. Combined with the browning phenotype of 212 lotus rhizomes, (epi) catechin, norarmepavine, and N-feruloyl-3-methoxytyramine were used as predominant chemical markers to separate different degrees of lotus rhizome browning. p-Coumaroyltyramine and N-trans-feruloyltyramine were selected as predominant chemical markers to investigate the differential expression between tropical and temperate lotus using principal component analysis and orthogonal partial least squares discriminant analysis. Shared and unique structure plots were used to compare the outcomes of the ecotype and browning OPLS model, showing that variation in tropical lotus rhizome browning is not obvious; this will be of great importance for genetic improvement by providing a hereditary basis.

Effect of different drying methods on drying kinetics, modeling, energy‐economic, texture profile, color, and antioxidant of lotus rhizomes ( Nelumbo nucifera )

The study was conducted on lotus rhizomes to find the kinetics of drying and ultrasound-assisted extraction process of antioxidant, energy-economic, quality parameters, and phytochemicals, under different drying processes. Midlli et al. model was found the best-fitted drying model with a low SSerror (0.0145–0.0088), RMSE (0.02 to 0.005), and highest R2 (0.999 to 0.992) value. The energy consumption cost (1518.33 Rs) is high in tray dryers and low in microwave dryers (28.68 Rs). The vacuum-dried lotus rhizomes had a high content of total phenolics of 78.364 mg/g, flavonoids of 10.819 mg/g (dm) content, DPPH of 74.621 μmol/g, ABTS of 104.36 μmol/g, and FRAP of 108.312 μmol/g. Low hardness (12.642 N), resilience (0.468), and high whiteness (61.158) were observed in freeze-dried rhizomes. Microwave and vacuum drying were showing a short time drying process with better energy economy as well as higher antioxidant content, but quality parameters are best for a freeze-drying product. Novelty impact statement The use of waste parts of plants as a source of functional food requires intensive study. Lotus rhizomes are edible and can have a vast range of applications in the food and pharmaceutical industries. The novelty of this study is that it explores different drying methods of lotus rhizomes and makes a comparative analysis of these methods based on their costs and effects. In addition, it also discusses the extraction of both free and bound phenolic compounds. The extraction of bound phenolic compounds increases the antioxidant content of the samples. Physicochemical properties (texture and color) of lotus rhizomes are also discussed in detail.

Expression rewiring and methylation of non-coding RNAs involved in rhizome phenotypic variations of lotus ecotypes

Non-coding RNAs (ncRNAs), including miRNAs, lncRNAs, and circRNAs, emerge as crucial components for gene regulation. Nelumbo nucifera (lotus), a horticulturally important plant, differentiates into a temperate ecotype of enlarged rhizomes and a tropical ecotype of thin rhizomes. Nevertheless, whether and how ncRNAs can be rewired in expression and differentially methylated contributing to adaptive divergence of this storage organ in lotus ecotypes is unclear. Herein, we study the expression behaviors and DNA methylation patterns of ncRNAs in temperate and tropical lotus rhizomes. By whole transcriptome sequencing, we found both mRNAs and lncRNAs have divergent expression patterns between ecotypes, whereas miRNAs and circRNAs tended to be accession-specific or noisier in expression. The differentially expressed ncRNAs are involved in phenotypic differentiation of lotus rhizome between ecotypes, as the genes that interacted with them in the competing endogenous RNA network are enriched in functions including carbohydrate metabolism and plant hormone signaling, being critical to rhizome enlargement. Intriguingly, ncRNA-targeted genes are less prone to show positive selection or differential expression during ecotypic divergence due to constraints from ncRNA-mRNA interactions. The methylation levels of ncRNAs generally tend to be higher in temperate lotus than in tropical lotus, and differential methylation of lncRNAs also tends to have expression changes. Overall, our study of ncRNAs and their targets highlights the role of ncRNAs in rhizome growth variation between lotus ecotypes through expression rewiring and methylation modification.

Comparative Analysis of Volatile Flavor Compounds of Different Pot-stewed Lotus Rhizomes by SPME-GC-MS and E-nose

Electronic nose and gas chromatography-mass spectrometry (GC-MS) were used to analyze the volatile flavor compounds of pot-stewed lotus rhizomes made in the laboratory (PSLR) and other three samples sold in the market. Principal component analysis (PCA) of the electronic nose and GC-MS data suggested that lotus rhizome stewed in laboratory has unique flavor. There were 65 kinds of volatile flavor components in PSLR (sample A), 51 kinds in sample B, 49 kinds in sample C and 41 kinds in sample D. There were 25 kinds of common components in four pot-stewed lotus rhizomes, such as β - myrcene, d-limonene, β - ocimene, α - terpinene, γ – terpinene, linalool, (-) - 4-terpineol, α - terpineol, anethol and eugenol. The principal component analysis (PCA) can find out the characteristic components which determine the flavor of different pot-stewed lotus rhizome products, and the overall flavor of PSLR samples is better than that of market products.

LAMP Assay for Distinguishing Fusarium oxysporum and Fusarium commune in Lotus (Nelumbo nucifera) Rhizomes

Yields of edible rhizome from cultivation of the perennial hydrophyte lotus (Nelumbo nucifera) can be severely reduced by rhizome rot disease caused by Fusarium species. There is a lack of rapid field-applicable methods for detection of these pathogens on lotus plants displaying symptoms of rhizome rot. Fusarium commune (91%) and Fusarium oxysporum (9%) were identified at different frequencies from lotus samples showing symptoms of rhizome rot. Because these two species can cause different severity of disease and their morphology is similar, molecular diagnostic-based methods to detect these two species were developed. Based on the comparison of the mitochondrial genome of the two species, three specific DNA loci targets were found. The designed primer sets for conventional PCR, quantitative PCR, and loop-mediated isothermal amplification (LAMP) precisely distinguished the above two species when isolated from lotus and other plants. The LAMP detection limits were 10 pg/μl and 1 pg/μl of total DNA for F. commune and F. oxysporum, respectively. We also carried out field-mimicked experiments on lotus seedlings and rhizomes (including inoculated samples and field-diseased samples), and the results indicated that the LAMP primer sets and the supporting portable methods are suitable for rapid diagnosis of the lotus disease in the field. The LAMP-based detection method will aid in the rapid identification of whether F. oxysporum or F. commune is infecting lotus plants with symptoms of rhizome rot and can facilitate efficient pesticide use and prevent disease spread through vegetative propagation of Fusarium-infected lotus rhizomes.

NnABI4-Mediated ABA Regulation of Starch Biosynthesis in Lotus (Nelumbo nucifera Gaertn).

Starch is an important component in lotus. ABA is an important plant hormone, which plays a very crucial role in regulating plant starch synthesis. Using ‘MRH’ as experimental materials, the leaves were sprayed with exogenous ABA before the rhizome expansion. The results showed that stomatal conductance and transpiration rate decreased while net photosynthetic rate increased. The total starch content of the underground rhizome of lotus increased significantly. Meanwhile, qPCR results showed that the relative expression levels of NnSS1, NnSBE1 and NnABI4 were all upregulated after ABA treatment. Then, yeast one-hybrid and dual luciferase assay suggested that NnABI4 protein can promote the expression of NnSS1 by directly binding to its promoter. In addition, subcellular localization results showed that NnABI4 encodes a nuclear protein, and NnSS1 protein was located in the chloroplast. Finally, these results indicate that ABA induced the upregulated expression of NnABI4, and NnABI4 promoted the expression of NnSS1 and thus enhanced starch accumulation in lotus rhizomes. This will provide a theoretical basis for studying the molecular mechanism of ABA regulating starch synthesis in plant.

Discovery of an effective processing method for edible rhizome to enhance the gamma-aminobutyric acid content

This study aimed to discover a simple, rapid, effective, inexpensive, and natural method to enhance the GABA content in edible rhizomes. To achieve this aim, edible rhizome samples were treated with different processing methods. Drying with hot air increased the GABA content in Chinese yam (CY) eight-fold compared with untreated CY. Drying with sunshine or hot air increased it 13- and 28-fold in taro root, respectively. These processing methods also produced an apparent increase in the GABA contents in other edible rhizome, such as lotus rhizomes, potatoes, and sweet potatoes. However, lyophilization did not affect the GABA content. Further, HPLC data showed that while GABA levels increased, glutamate levels decreased, indicating that GABA is produced by the catalytic action of glutamate decarboxylase on glutamate under drought conditions. Drying with hot air or sunshine to enhance the GABA content was not indicated in the literature.

Physico-mechanical characterization of different grades of Lotus rhizome (Nelumbo nucifera Gaertn) for valorisation and smart post-harvest management

The debilitated attention in post-harvest management of lotus rhizomes (Nelumbo nucifera Gaertn) is of serious concern to the food industry. Far too often, exposure to mechanical damage during post-harvest handling, transportation, and valorisation of lotus rhizomes scores low in its quality and leads to post-harvest losses, hence the detailed profiling of this biomaterial is much needed. In this study, the data for physical and mechanical/ engineering properties of lotus rhizomes of varying sizes were generated to facilitate its utilisation in designing post-harvest and processing machinery and also planning its processing and valorisation. Lotus rhizomes were classified into different sizes for assessing the requirement of grading and sorting devices in the future. The significant difference (P<0.05) in length, width, thickness, L*, a*, and b* values ranged from 16.02 to 39.00 cm, 1.13 to 3.50 mm, 1.03 to 3.30mm, 49.10 to 64.20, 5.90 to 2.80, 6.77 to 18.01 respectively, which indicates less wastage with the increase in sizes. Besides, colour saturation, colourfulness, and purity improve with the increase in rhizome size. The mechanical properties are also influenced by the presence of air canals (pores) in the rhizome compared to other cylindrical vegetables and also suggest that the rhizomes of different sizes should be handled separately. The minimum pressure forces during handling should be in the range of 9.28 to 80.57 N depending upon the size and the shear force ranged from 100.40 to 167.62 N which increased with the increase in the size of rhizomes. Similarly, hardness values of TPsA have increased with the increase in size. The results obtained from observing these properties potentials to be useful in reducing wastages and manage smart postharvest operations in the future.

Acetic acid reducing the softening of lotus rhizome during heating by regulating the chelate-soluble polysaccharides

Lotus rhizomes were used to study on the relationship between the cell wall polysaccharides and cooked texture by adding acetic acid. Hardness and scanning electron microscopy results showed that acetic acid treatment can maintain higher hardness and the integrity of the cell wall. Then, the cell walls were sequentially extracted and divided into water-soluble fraction, chelate-soluble fraction (CSF), sodium carbonate-soluble fraction and hemicellulose fraction. The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group. The nanostructures showed that acetic acid treatment maintained longer chain and destroy helical structure of CSF backbone. This work helps us to demonstrate the relationship between polysaccharides structure and cooked texture, and further control the plant-based vegetables processing texture in food industry.

Physicochemical Properties of Starches from Lotus Rhizomes Harvested in Different Months.

We investigated the physicochemical properties of starches extracted from 8 lotus (Nelumbo nucifera Gaertn.) rhizomes harvested in different months (September 2012 to May 2013). The physicochemical properties of the lotus starches depended on the harvest date. The peak viscosity (PV) in the Rapid Visco-Analyser analysis, and the viscosity at 65 °C (V65) in the rotational viscometer analysis were significantly lower in SEP starch (extracted from the September-harvested sample) than in the other lotus starches. The Spearman’s rank correlation coefficients of potassium ion (K) content vs. V65 and of K content vs. PV were 0.905 and 0.714, respectively, indicating that potassium ions are important for expressing the pasting properties of lotus starch. Principal component analysis suggested that the potassium, magnesium, calcium, and phosphorus contents are important for displaying both the pasting and gelatinization properties of the lotus starches. Meanwhile, the cluster analysis revealed that physicochemical properties of the SEP starch were different from those of the starches harvested in other months.

The ratio of chelate-soluble fraction to alcohol insoluble residue is a major influencing factor on the texture of lotus rhizomes after cooking

The differences in cell wall polysaccharides are considered as a major influencing factor on the texture of plant-based food after cooking. Here, 18 varieties of lotus rhizomes were collected from different regions of China and subjected to analysis, with the aim to identify the key factors that affect the texture of lotus rhizomes after cooking. The texture (hardness) of fresh samples and the samples after thermal treatment for different time periods was examined. The cell wall polysaccharides present in alcohol insoluble residue (AIR) were further subdivided into different fractions, and the composition of monosaccharides in each fraction was analyzed by gas chromatography. We then calculated the sugar ratios to examine the discrepancies in molecular structure among the fractions. Principal component analysis and regression analysis showed that the ratio of chelate-soluble fraction (CSF) to AIR is the major factor affecting the texture of lotus rhizomes after cooking.

Cell wall CSF/WSF ratio value discriminates the cooked texture of lotus rhizome

ABSTRACTIn order to find out the key factors to affect the texture variation and the formation mechanism of different cultivars lotus rhizomes after thermal processing, representative lotus rhizomes harvested in year round with 2 months interval, including Lulinhu, Miancheng, and Elian 5, were chosen to calculate the cause of thermal process texture. The texture (hardness), content of different fractions of alcohol insoluble residue (AIR), and microstructure of the cell wall components of the lotus rhizomes exhibited changes during processing. The changes of three pectin fractions in the three lotus rhizomes cultivars were compared by combining the effect of thermal treatment on the lotus rhizome AIR pectin content degree of esterification (DE) and analysing the texture and microstructure, the cell wall materials were found to be the crucial factor in this process. Another six cultivars lotus rhizomes have been employed to replicate the above mentioned results, the total data were used to calculate the chelator soluble fraction (CSF) to water soluble fraction (WSF) ratio value (0.25), which determines the texture of the lotus rhizomes after thermal-processing. The CSF/WSF ratio values (0.25) will give an index for the lotus rhizome processing food industry.

Prescreening Consumer Acceptance for Edible Lotus Rhizome

Lotus (Nelumbo nucifera Gaertn.) is an underused edible aquatic perennial vegetable currently evaluated as a potential functional food source and promoted in the southeastern United States as a rich source of phytonutrients. There is a paucity of information concerning consumer acceptance and willingness to purchase edible, value-added lotus products in the southeastern United States. The purpose of this exploratory study was to evaluate the potential demand and consumer preference for fresh lotus rhizomes and value-added products namely lotus salad, baked lotus chips, and lotus stir-fry. Results of two taste panels indicated that lotus stir-fry was the most preferred value-added product with 77% of participants strongly liking stir-fry, whereas 92% of the participants were willing to recommend this preparation. Results suggest socioeconomic characteristics such as gender (P = 0.014), age (P = 0.005), income (P = 0.043), education (P = 0.003), shopping habits (P = 0.013), and type of meal purchased (P = 0.004) are the factors affecting consumer choice and willingness to recommend lotus stir-fry. Results provide information on consumer acceptance of fresh lotus rhizomes and value-added products. Findings of this case study will assist in analyzing consumer behavior and development of sustainable niche markets for locally cultivated fresh edible lotus rhizomes.