Lotion Preparation Research Articles

Formulation and Physical Evaluation of Handbody Lotion Preparations Niacinamide with a combination of Alpha Arbutin as brightening

Formulation and Physical Evaluation of Handbody Lotion Preparations Niacinamide with a combination of Alpha Arbutin as brightening

Potential and Activities of the Sunscreen Lotion Preparation of Bawang Dayak (Eleutherine americana (Aubl.) Merr. by Ethanol Extract Fraction from East Boneo

Has conducted research Potential and Activities of the Sunscreen Lotion of Bawang Dayak (Eleutherine americana (Aubl.) Merr. by ethanol extract fraction from East Borneo. The purpose of the study was to determine the potency ann activity of the sunscreen ethanol extracts fraction of Bawang Dayak in the preparation of a lotion with the parameter measuring the percent transmission erythema (% Te) and pigmentation (% Tp) in vitro in the room temperature 27oC storage and accelerated temperature 40oC. Lotion dosage formulations using ethanol extracts fraction bawang Dayak with a concentration of 0.1% and 0.2%, and positive control is a trademark preparation Bless Sunscreen Lotion SPF 15®. Stages of research include the formulation and measurement of absorbance and value %Te) and %Tp at various storage temperatures with UV-Vis spectrophotometry at λ 292.5 to 372.5 nm. The analysis of research data using a program SPSS. The results showed that ethanol extract fraction concentration in lotion preparations of bawang Dayak 0.1% and 0.2% active as a sunscreen included in the category of sunblock. The extract fraction is active as a sunscreen and in the Sunbloc category, with the best concentration as a sunscreen being 0.2%, with its sunscreen potential not being significantly different from the control Sunscreen SPF 15® at the 5% level.

FORMULATION OF RED BETEL LEAF EXTRACT LOTION (Piper crocatum Ruiz & Pav) AS SUNSCREEN

Betel leaf is a natural ingredient that people use as a traditional medicine. One of the ingredients found in red betel leaves is a phenolic compound in the form of flavonoids, a photoprotective substance that can be used as protection from UV radiation. Sunscreen is a cosmetic preparation that can protect the skin from UV radiation and reduce the amount of radiation harmful to the skin. This study aimed to formulate a lotion preparation from red betel leaf extract that has activity as a sunscreen. Red betel leaf (Piper crocatum Ruiz & Pav) was extracted using the maceration method. Preparation formulations were made with variations of red betel leaf ethanol extract in each formula, namely with concentrations of 0.04% (F1), 0.05% (F2), 0.06% (F3), and base control (F0). The activity of sunscreen lotion extract is based on the Sun Protection Factor (SPF) value using UV-VIS spectrophotometry at wavelengths of 290–320 nm using the Mansur equation. The results of the SPF value of the lotion are F1 of 13.48, F2 of 16.71, and F3 of 18.7. The evaluation of the lotion results obtained a light green lotion with M/A emulsion type that met the requirements, including homogeneity, pH, spreadability, viscosity, centrifugation test, and freeze and thaw test. Research results show that betel leaf extract lotion is physically stable and has a high SPF value, so it has the potential to be a sunscreen.

The Antioxidant Activity of Avocado (Persea americana Mill) Seed Extract and Lotion Preparation

Excessive exposure to free radicals can cause skin to look dry and thin. Avocado seeds are a part of the fruit that is very rarely used and is even considered waste by society. Avocado seeds are known to contain good antioxidant activity to ward off free radicals. Topical preparations that are widely used to protect the skin from exposure to free radicals, one of which is lotion preparations with natural ingredients that contain antioxidants. This research aims to determine the antioxidant activity of avocado seed extract with different concentrations, formulate avocado seed extract lotion, distribute avocado seed extract lotion preparations, and determine the antioxidant activity of avocado seed extract lotion. The method used in this research is the in vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Each avocado seed was extracted with ethanol 50 %; 70 %; and 96 %. The best antioxidant results are made into cream preparations in 3 formulas, namely F1 0.01 %; F2 0.05 %; and F3 0.1 %. The best antioxidant test results were obtained from avocado seed extract with ethanol 70 % and an IC50 of 20.68 ppm with very strong antioxidant intensity. The three lotion formulations met the evaluation requirements for topical preparations and provided an antioxidant value of F1 of 128 ppm, F2 of 72 ppm, and F3 of 55 ppm. The conclusion of this research is that the highest antioxidants in avocado seed extract are found in the 70 % ethanol extract and in the formula 3 lotion preparation.

Analysis of Hydroquinone Compounds in Whitening Body Lotion Preparations Circulating in The Community

Skin care has become an important part for today's women. Whitening body lotion is very necessary in maintaining skin when doing activities outside and indoors. Many Indonesian women desire to have white, clean and bright skin so they can appear attractive. An attractive appearance is very necessary in society. To achieve this, many women choose skin care products at affordable prices without paying attention to the dangers of the chemical content in them. One of the skin whitening ingredients that is often used in beauty products is the compound hydroquinone. The hydroquinone compound plays a role in preventing skin pigmentation. However, if the levels exceed 0.02% in the preparation, it will cause skin irritation, kidney function disorders and cancer. The type of research is a mixed type, namely qualitative and quantitative. Qualitatively, it will obtain data on the presence of hydroquinone and quantitatively in the form of hydroquinone levels. The samples were 5 whitening body lotion preparations circulating in Lubuk Pakam which were determined randomly and were coded A, B, C, D, and E. Qualitative analysis was carried out using the TLC method and quantitative analysis was carried out using the UV-spectrophotometric method. Visible. The results of qualitative analysis show that samples A, C, and D contain hydroquinone compounds which are characterized by the appearance of blackish brown spots with respective Rf values of 0.89; 0.96; and 0.89. Meanwhile, when qualitative analysis was carried out, the levels obtained for the three samples were 44.56% each; 5,604%; and 5,612%. Based on regulations issued by BPOM RI in 2022, it is stated that hydroquinone levels in beauty products must not exceed 0.02%. So it can be concluded that whitening body lotion preparations with sample codes A, C, and D are not recommended for use because the use limit for hydroquinone is classified as unsafe. It is recommended that people, especially women, be more selective in choosing and using beauty products.

Antioxidant and Anti-inflammatory Potential of Sargassum sp Extract in Lotion Preparations

Sargassum sp. is a type of brown algae found in Indonesian marine waters with great potential in the food and pharmaceutical industries. There is antioxidant and anti-inflammatory potential in Sargassum sp. extract so that it makes this type utilised as a lotion preparation. It is necessary to know the potential of Sargassum sp. extract in making lotions. This research uses extraction method to produce Sargassum sp. extract with organic solvent extraction technique, namely alcohol. In addition, the emulsion method was used in the manufacture of lotion preparations. Furthermore, secondary metabolites and anti-inflammatory (irritation) tests were conducted. In addition, the lotion preparation can be evaluated by conducting organoleptic, pH, and homogeneity tests. The results showed that the Sargassum sp. extract contained significant antioxidant compounds, as indicated by a decrease in DPPH absorbance. Sargassum extract contains alkaloid, flavonoid, saponin, and tannin. In addition, lotion preparations containing Sargassum sp. extract also showed a significant decrease in pro-inflammatory cytokine expression in skin cells exposed to UVB radiation. In addition, the irritation test showed negative results with a safety level without causing irritation to the skin. These findings indicate the potential of Sargassum sp. extract in producing lotion preparations with antioxidant and anti-inflammatory effects that can contribute to skin protection from oxidative damage and inflammation.

Formulation and Sunscreen Activity Test of Lotion of Buas-Buas (Premna Serratifolia Linn.) Leaf Extract

Excessive UV exposure can cause various skin disorders, so protection from UV rays is needed by using sunscreen. One of the plants that have the potential as a sunscreen is buas-buas leaf (Premna serratifolia Linn.) which contains flavonoid compounds. Sunscreen formulated in lotion form is the most common preparation found in the market. The purpose of this study was to evaluate the physical characteristics of lotion preparations and to determine the potential of lotion of buas-buas leaf extract as a sunscreen. Buas-buas leaf extract was obtained through maceration extraction with 96% ethanol solvent. Lotion with the active substance of buas-buas leaf extract was made with concentration variations of 1, 2, and 3%. Evaluation of the physical characteristics of the lotion was tested with organoleptic observation parameters, homogeneity, pH, spreadability, and adhesion. Then, it was tested for sunscreen potential using UV-Vis spectrophotometry. Data were analyzed with SPSS Statistics 22 software, namely the One Way ANOVA (Analysis of Variance) test. The results of the study were the three lotion formulas of buas-buas leaf extract met all the requirements of the physical characteristics evaluation test. The SPF value of F1 (1%) is 7.12 (extra protection), F2 (2%) is 9.54 (maximum protection) and F3 (3%) is 15.68 (ultra protection). Based on the results of the study, it can be concluded that the lotion of buas-buas leaf extract meets the requirements of evaluating the physical characteristics of lotions and has activity as a sunscreen.

Effectiveness of repellent from patchouli (Pogestemon cablin) varieties of Southeast Sulawesi against Aedes aegypti.

The incidence of Dengue Hemorrhagic Fever (DHF) continues to increase over time in the world, including Indonesia. One of the prevention efforts against dengue virus transmission is to avoid vector mosquito bites by the use of repellants. Using repellents can reduce exposure to mosquito bites that may cause infection with the dengue virus. This study aimed to determine the effectiveness of repellent lotion composed of patchouli batik extract (Pogostemon cablin) from Southeast Sulawesi varieties against Aedes aegypti mosquitoes. The research subjects were Aedes aegypti adult mosquitoes. The research consisted of three stages. The first stage was a phytochemical test (qualitative method), the second stage was the analysis of patchouli essential oil (GC-MS method) and the third stage was a test of the effectiveness of lotions made from patchouli extract in lotion preparations against Aedes aegypti. The results of the effectiveness test of patchouli leaf repellent (Pogestemon cablin) lotion preparations were as follows: for a concentration of 2.5%, protective power 81.5%; concentration 5%, protection power 83.67%; concentration 7.5%, protection power 88.64 %; the concentration of 10%, protection power 90.44%, and the concentration of 12.5% had protection power 90.89%. Probit analysis and linear regression showed the value of ProbitLc 50 was 6.631. The results of the effectiveness test of Pogestemon cablin repellent lotion preparations with the most effective concentrations were 10% and 12.5%. The results of ANOVA test indicated there was no difference in the average value of the protection power in patchouli leaves.

Physical Quality Evaluation and Determination of SPF (Sun Protection Factor) Value of Forest Coconut Oil Lotion (Pandanus julianettii Martelli) from Papua

Forest coconut fruit contains double-bonded fat or omega 3-6, tocopherol, or vitamin E. Vitamin E and some compounds such as flavonoids, tannins, and anthraquinone have potential as sunscreens. This study aims to evaluate the physical quality and determine the SPF value in forest coconut oil lotion analyzed in vitro. Physical quality evaluation includes organoleptic tests, homogeneity, spreadability, adhesion, pH, and the determination of SPF values by the UV-Vis spectrophotometry method. The results showed that each formula, namely FI (2%), FII (4%), and FIII (6%), was physically stable. The lotion preparation in each formula, namely FI (2%), FII (4%), and FIII (6%), has SPF values of 1.95, 1.57, and 1.19, respectively.

Potential Test of Jojoba Oil-Based Sunscreen in Lotion Preparations

This study aimed to determine the formula of anti-aging cream using lemongrass leaf extract. The treatment by giving respondents lemongrass leaf extract and using moisture, evenness, pore, spot, and wrinkle as parameters. Human skin provides protection on the surface of the body against the influence of the surrounding environment. Skin can experience aging, especially in areas that are often exposed to direct sunlight such as the face, neck, upper arms, and hands. Aging is a process experienced by humans in their 30s whose functions in the human body are decreasing, for example, thinning skin, wrinkles, rough skin, and dark spots on the face. Treatment to prevent aging of the skin by using an antioxidant product such as flavonoids because it has the function of preventing or neutralizing free radicals. In Indonesia, several species of plants can be found that function as food and cosmetic ingredients. The lemongrass leaf extract which contains antiaging are Alkaloids, Flavonoids, and Tannins. The data were analysed by using the Kolmogorov-Smirnov method to determine homogeneity and normality. Then, if the data is normal, it is continued to be analysed using the One-Way ANOVA method to determine the average difference between groups. There is a difference between treatments, and it is continued with the Post-hoc Tukey HSD test. The higher the concentration of extract used, the greater the anti-aging productivity formed. It is concluded, the best anti-aging effect is a concentration of 9% with increase in moisture, evenness, pore reduction, reducing spots and wrinkles.

UJI STABILITAS FISIK LOTION ANTI NYAMUK KOMBINASI MINYAK LAVENDER (Lavandula angustifolia) DAN MINYAK ROSEMARY (Rormarinus officinalis)

Lavender essential oil 15% can be used as an anti-mosquito protection power on average 56% at 6 hours. Rosemary has a more fragrant aroma and can make mosquitoes uncomfortable and avoid it. This study aims to determine physical stability of mosquito repellent lotion. Lavender oil mosquito repellent lotion (Lavandula angustifolia) and rosemary oil (Rosmarinus officinalis) made in 3 formulas, namely F1 containing lavender oil (10%), F2 (15%), and F3 (20%), and F1 containing rosemary oil (30%), F2 (25%), and F3 (20%). Mosquito repellent lotion was tested for its physical stability using the cycling test method which included organoleptic, homogeneity, pH, adhesion, and spreadability. In this research, citronella and lavender oils can be formulated into anti-mosquito lotions. The results of physical stability (organoleptic, pH, homogeneity, spreadability and adhesion) using the cycling test method show that the anti-mosquito lotion did not change during storage and the results were in accordance with the expected specifications. It can be concluded that the difference in the concentration of lavender and rosemary essential oils does not affect the stability test of the anti-mosquito lotion preparation.

PENGARUH VARIASI KONSENTRASI SETIL ALKOHOL SEBAGAI EMULSIFYING AGENT PADA SEDIAAN LOTION EKSTRAK LIDAH BUAYA (Aloe vera (L.) Burm. f)

Lotion is a liquid emulsion consisting of an aqueous phase and an oil phase that is stabilized by an emulsifying agent and contains one or more active ingredients. The use of cetyl alcohol as an emulsifying agent was selected because of its properties as a softener, emulsifier, and absorbing water. Cetyl alcohol can increase the stability of the consistency and improve the texture. This study aims to determine the effect of concentration variations in cetyl alcohol on the physical properties of aloe vera extract lotion. The research design used in this study is a pre-experimental preparation formula lotion that was divided into 3 sample groups with different concentrations of cetyl alcohol, namely (2%), (3%), and (4%). Then the sample will be tested for physical properties including organoleptic tests (color, texture, flavor), pH test, spreadability test, and viscosity test, homogeneity test. The research results showed that variations in cetyl alcohol concentrations affected the physical properties of the preparations organoleptic texture, and viscosity. Lotion preparations do not affect organoleptic color, flavor, pH, and homogeneity. Formulation with concentration variation of 3% cetyl alcohol had good physical properties and met the criteria including organoleptic testing with a milky white color, slightly viscous texture, strong jasmine flower flavor, pH value of 7.16 ± 0.34, spreadability value 6.99cm ± 0.18, viscosity value 31.6 dPas ± 2.88, and homogeneous. 
 
 Keywords: lotion, cetyl alcohol, Aloe vera

A case study on Manjalkaram (medicated alkaline lotion) in the management of Powthiram (fistula-in-ano)

Abstract Background: Abscesses around the lower rectum and anal canal are known as anorectal abscesses. These are crucial as they often culminate in fistula-in-ano. Anorectal abscess and fistulas are one of the most frequent results in the manifestation of simple or complex and extensive fistulous tracts. Karam (herbo-mineral preparation) therapy is a Siddha parasurgical technique commonly used to cure anal disease. Materials and Methods: Manjalkaram is a medicated alkaline lotion preparation that acts as herbal chemical cauterization agent to cure anal disease. A 60-year-old male patient, already treated with allopathy, experienced a recurrence of fistula-in-ano. The patient decided to get Siddha treatment and presented with the complaints of pain in the anal area, swelling, and discharge of pus with stool and mild irritation. The patient was diagnosed with fistula-in-ano. Purgatives were given the previous night to clean the bowel, and the following day, local cleaning was carried out by providing Triphala decoction as a herbal antiseptic lotion. A probe was inserted into the fistula, and a Manjalkaram-soaked medicated thread was inserted through the fistula and tied through an external opening to the internal opening with help of the probe. A Manjalkaram-soaked gauze (Manjalkaraseelai) was inserted and changed once in a week. Result: Even though fistula-in-ano is very difficult to treat, the patient got completely healed within 2 months with the effect of Manjalkaram application. Conclusion: Manjalkaram application is found to be an effective, time-saving, affordable, and acceptable treatment for fistula-in-ano.

Characteristics of mosquito-repellent lotion made from pineapple peel waste

Pineapple peel can be used as a natural ingredient in making mosquito-repellent lotion because it contains many secondary metabolite compounds, including saponins, tannins, flavonoids, alkaloids, steroids, and essential oils. The research aims to obtain the right concentration of pineapple peel extract on the characteristics of the mosquito-repellent lotion produced. The method used was an experimental method with a Completely Randomized Design (CRD) consisting of five treatments (2%, 3%, 4%, 5%, and 6% pineapple peel extract) with three replications to obtain 15 experimental units. The selected treatment was LN2 (lotion with a 3% concentration of pineapple peel extract), providing homogeneous physical characteristics of the lotion preparation, a pH value of 7.93, a spread ability value of 5.50 cm, a viscosity value of 3829.51 cP, and mosquito protection power 80.16%. The descriptive organoleptic assessment showed that the color of the lotion was yellowish brown, slightly scented with citronella, and slightly sticky in texture, and the panelists liked the hedonic evaluation of color and texture. The panelists little appreciated the hedonic aroma.

DAYA PROTEKSI LOTION DENGAN MINYAK ATSIRI SERAI DAPUR (Cymbopogon citratus) TERHADAP NYAMUK Aedes aegypti

Lemongrass consists of two types that have many benefits. Its use in research or in processing using its stems alone causes lemongrass leaf waste which can be used as an essential oil and used as an anti-mosquito lotion to reduce the use of chemicals as repellent. The purpose of this study was to determine the yield of lemongrass essential oil, to determine the protective power of each concentration of citronella essential oil in lotion in repelling mosquito bites, and to determine the level of preference for mosquito repellent lotion. The average yield with three repetitions followed by standard deviation (SD) was 0.1% ± 0.0024% b/b. Lotion preparation provides different protection during 6 hours of exposure, namely K- and F1 no protection, F2 for 2 hours, F3 and K+ 4 hours and F4 5 hours. Based on all parameters, the most preferred formulation is F1 and does not give sensitive reactions. Keyword: Aedes aegypti, essential oils, lemongrass, lotions.

Formulation of Seaweed (Eucheuma cottonii Doty) Lotion

Seaweed (Eucheuma cottonii Doty) has potential as an antioxidant because it contains kappa-carrageenan which can counteract free radicals. Formulation of seaweed (Eucheuma cottonii Doty) into lotion preparations can minimize the negative effects of sun exposure, because it can reflect solar radiation or work as a physical sunscreen. To give the maximum effect, particle size reduction is carried out, so that the smaller the particle size of the seaweed powder, the surface area increases. This study aims to determine the formulation and evaluate the preparation of lotion from seaweed powder. This research was conducted using an experimental method. The lotion was made with three variations of seaweed powder concentration (Eucheuma cottonii Doty), namely 2.5%, 3% and 5%. Tests were carried out before and after accelerated conditions at 5oC and 35oC for 12 hours each for 12 cycles with test parameters including organoleptic, homogeneity, pH, spreadability, viscosity and flow type. The results obtained in the organoleptic test showed no change in the three formulas both before and after the accelerated condition. The pH test showed that Formula 2 and Formula 3 still showed that the preparation remained in the skin pH range for sunscreen. In the homogeneity test, all preparations were homogeneous, as well as the spreadability test met the test requirements criteria. Based on the tests carried out, it can be concluded that formulas 2 and 3 have optimum stability.

Karakteristik Lotion Anti Nyamuk Kombinasi Ekstrak Sabut Kelapa (Cocos nucifera) dan Bunga Kamboja (Plumeria sp.)

Mosquitoes are vectors of several diseases, one of which is Dengue Hemorrhagic Fever. Therefore, it is necessary to take steps to prevent the spread of the disease. One of them by using anti-mosquito lotion. However, some of commercial lotions are made of chemicals that have side effects on health. Therefore, coconut coir (Cocos nucifera L.) and frangipani flower anti-mosquito lotion was developed. Coconut coir contains tannins which repel insects and have a therapeutic effect. Frangipani flowers contain geraniol which can repel insects. The purpose of this study was to determine the characteristic of coconut coir extract (Cocos nucifera L.) with a combination of frangipani flower extract (Plumeria sp.) as a lotion preparation. This research is an experimental study with 7 treatments of lotion formulation.. The data in this study include organoleptic tests, homogeneity tests, pH, and spreadibility of the lotion. The conclusion of this study showed that a lotion formula P3 with a ratio of 1:2 (coconut coir extract:frangipani flower extract) was the best formulation with the characteristics of a fine solid texture, brownish yellow color, frangipani fragrant, homogeneous, have pH 6, and the diameter of spread is 7,5cm.

STUDY OF THE ESSENTIAL OIL OBTAINED FROM JUNIPER PLANT BY PHYSICAL-CHEMICAL METHODS

Plants are natural resource with a perfect structure that can meet many needs of people and show diversity since hundreds of years. All over the world and in our country, plants have been used for a long time in the preparation of tea, spices, perfumes, ointments and medicines used in the treatment of diseases. Essential oils arelarge group of biologically active compounds contained in plants. In the article, the process of extracting essential oil from the juniper plant (Juniperus communis L.) at different yields was studied. Juniper fruit 200.47 g; 200.28 g; when 200.54 g, the average amount of essential oil according to wet raw material was determined to be 1.2±1.4%. The composition of the essential oil obtained from juniper fruit, rich in biologically active substances and important for treatment, was studied by physicochemical methods (chromatographic, Ultraviolet (UV) and Infrared (IR) spectroscopic). In the chromatogram of the essential oil, it was determined that there is a peak of monoterpenoids with a tricyclic structure. Absorption bands obtained as a result of spectral analysis ensure the correctness of the composition of the synthesized essential oil. On the basis of medicinal properties of the juniper plant (Juniperus communis L.), the obtained essential oil can be used in medicine and cosmetology in the preparation of ointments and lotions with various ingredients.

Formulasi dan uji aktivitas sediaan lotion tabir surya ekstrak etanol 96% bekatul beras merah (Oryza nivara)

Brown Rice Bran (Oryza nivara) contains phenolic compounds, flavonoids, tannins, steroids, or terpenoids that can function as an antioxidant activity that can protect the skin from the adverse effects of UV rays of the sun. Extraction was carried out by maceration method using 96% ethanol as solvent. The viscous extract obtained was then subjected to phytochemical screening and SPF test and made into three different concentrations, 5%, 10%, and 15%, which would then be formulated into a sunscreen lotion dosage form. The resulting preparations evaluated the physical quality of lotion preparations in the form of organoleptic tests, homogeneity tests, pH tests, dispersibility tests, and SPF tests of preparations. The results of the SPF test extract with concentrations of 5%, 10%, and 15% showed that the protection produced was in the ultra-category with SPF values respectively 31.1, 40.1, and 45.1. The results of the physical evaluation of the preparation of sunscreen lotion with brown rice bran extract showed that the lotion produced was not homogeneous, and the results of the pH test and dispersion test still met the requirements. Testing the SPF value of lotion preparations showed that the protection produced by each formula was in the maximum to ultra-category with SPF values of 11.7, 14.1, and 16.4, respectively.

Repurposing sunscreen as an antibiotic: zinc-activated avobenzone inhibits methicillin-resistant Staphylococcus aureus.

Methicillin-resistant Staphylococcus aureus (MRSA) is a major healthcare concern with associated healthcare costs reaching over ${\$}$1 billion in a single year in the USA. Antibiotic resistance in S. aureus is now observed against last line of defense antibiotics, such as vancomycin, linezolid, and daptomycin. Unfortunately, high throughput drug discovery approaches to identify new antibiotics effective against MRSA have not resulted in much tangible success over the last decades. Previously, we demonstrated the feasibility of an alternative drug discovery approach, the identification of metallo-antibiotics, compounds that gain antibacterial activity only after binding to a transition metal ion and as such are unlikely to be detected in standard drug screens. We now report that avobenzone, the primary active ingredient of most sunscreens, can be activated by zinc to become a potent antibacterial compound against MRSA. Zinc-activated avobenzone (AVB-Zn) potently inhibited a series of clinical MRSA isolates [minimal inhibitory concentration (MIC): 0.62-2.5 µM], without pre-existing resistance and activity without zinc (MIC: >10 µM). AVB-Zn was also active against clinical MRSA isolates that were resistant against the commonly used zinc-salt antibiotic bacitracin. We found AVB-Zn exerted no cytotoxicity on human cell lines and primary cells. Last, we demonstrate AVB-Zn can be deployed therapeutically as lotion preparations, which showed efficacy in a mouse wound model of MRSA infection. AVB-Zn thus demonstrates Zn-activated metallo-antibiotics are a promising avenue for future drug discovery.