Loss Of Resolution Research Articles

Single-Cell Lipidomics: An Automated and Accessible Microfluidic Workflow Validated by Capillary Sampling.

We report the first demonstration of a microfluidics-based approach to measure lipids in single living cells using widely available liquid chromatography mass spectrometry (LC-MS) instrumentation. The method enables the rapid sorting of live cells into liquid chambers formed on standard Petri dishes and their subsequent dispensing into vials for analysis using LC-MS. This approach facilitates automated sampling, data acquisition, and analysis and carries the additional advantage of chromatographic separation, aimed at reducing matrix effects present in shotgun lipidomics approaches. We demonstrate that our method detects comparable numbers of features at around 200 lipids in populations of single cells versus established live single-cell capillary sampling methods and with greater throughput, albeit with the loss of spatial resolution. We also show the importance of optimization steps in addressing challenges from lipid contamination, especially in blanks, and demonstrate a 75% increase in the number of lipids identified. This work opens up a novel, accessible, and high-throughput way to obtain single-cell lipid profiles and also serves as an important validation of single-cell lipidomics through the use of different sampling methods.

Intraocular lens simulator using computational holographic display for cataract patients.

To develop and validate a holography based vision simulator for the demonstration of expected postoperative vision corresponding to monofocal and multifocal intraocular lenses (IOL) to cataract patients before surgery. An artificial eye model is used to measure the optical performance of different IOL types. The resultant aberrations and degradations are then modeled using phase holograms and shown to subjects on a holographic display. We measure the contrast and resolution loss, halos around the light sources, and point spread function (PSF) corresponding to three different IOLs. We tested the holography based vision simulator on 13 healthy subjects and 6 cataract patients. Monofocal, bifocal, and trifocal IOLs exhibited a contrast decrease of 5%, 42%, and 45% and a resolution limit of 4.49, 4.00, and 4.00 lp/mm (using 0.05 MTF criteria), respectively. Monofocal IOLs have the best resolution and contrast at the optimal focus distance, and multifocal lenses offer extended depth-of-field but exhibit prominent halos and reduced contrast/resolution. We confirmed that the visual functions of IOLs could be successfully modeled using phase holograms and simulated using a holographic display without using a physical IOL. Patients can experience the effects of different IOL options prior to surgery, which helps with IOL selection, expectation management, and patient satisfaction.

Magneto-acousto-electrical tomography based on frequency response compensated linearly frequency-modulated signal stimulation

Objective. In magneto-acousto-electrical tomography (MAET), linearly frequency-modulated (LFM) signal stimulation uses much lower peak voltage than the spike pulse stimulation, lengthening the operation life of the transducer. However, due to the uneven frequency responses of the transducer, the low-noise amplifier (LNA), and the bandpass filter (BPF), MAET using LFM signal stimulation suffers from longitudinal resolution loss. In this paper, frequency response compensated linearly frequency-modulated (FRC-LFM) signal stimulation is investigated to resolve the problem.Approach. The physical model of measurement of the frequency responses of the transducer and the cascading module of the detection electrodes, the LNA, and the BPF is constructed. The frequency responses are approximated by fitting a curve to the measurement data. The frequency response compensation function is set to the reciprocal of the product of the frequency responses. The digital FRC-LFM signal is generated in MATLAB and converted to analog signal through an arbitrary waveform generator. Two groups of MAET experiments are designed to confirm the performance of the FRC-LFM signal stimulation. Pure agar phantom with rectangular through-holes and agar phantom with pork tissue inclusion serve as the samples.Main results. The pulse-compressed magneto-acousto-electrical signal obtained using FRC-LFM stimulation has narrower main-lobe than that obtained using LFM excitation, although the signal to noise pulse interference ratio of the former is little lower than that of the latter, which is due to the limitation of the power amplifier. FRC-LFM also proves to be an effective method to utilize the frequency outside the working band of the transducer in MAET.Significance. The method in this study compensates for the longitudinal resolution loss due to the uneven frequency responses. Combining with high-capability power amplifier and high-performance LNA, the MAET using FRC-LFM signal stimulation can potentially achieve high longitudinal resolution and high sensitivity, advancing MAET toward the clinical application.

Performance evaluation of a 4D similarity filter for dynamic CT angiography imaging of the liver.

Dynamic computed tomography (CT) angiography of the abdomen provides perfusion information and characteristics of the tissues present in the abdomen. This information could potentially help characterize liver metastases. However, radiation dose has to be relatively low for the patient, causing the images to have very high noise content. Denoising methods are needed to increase image quality. The purpose of this study was to investigate the performance, limitations, and behavior of a new 4D filtering method, called the 4D Similarity Filter (4DSF), to reduce image noise in temporal CT data. The 4DSF averages voxels with similar time-intensity curves (TICs). Each phase is filtered individually using the information of all phases except for the one being filtered. This approach minimizes the bias toward the noise initially present in this phase. Since the 4DSF does not base similarity on spatial proximity, loss of spatial resolution is avoided. The 4DSF was evaluated on a 12-phase liver dynamic CT angiography acquisition of 52 digital anthropomorphic phantoms, each containing one hypervascular 1cm lesion with a small necrotic core. The metrics used for evaluation were noise reduction, lesion contrast-to-noise ratio (CNR), CT number accuracy using peak-time and peak-intensity of the TICs, and resolution loss. The results were compared to those obtained by the time-intensity profile similarity (TIPS) filter, which uses the whole TIC for determining similarity, and the combination 4DSF followed by TIPS filter (4DSF + TIPS). The 4DSF alone resulted in a median noise reduction by a factor of 6.8, which is lower than that obtained by the TIPS filter at 8.1, and 4DSF + TIPS at 12.2. The 4DSF increased the median CNR from 0. 44 to 1.85, which is less than the TIPS filter at 2.59 and 4DSF + TIPS at 3.12. However, the peak-intensity accuracy in the TICs was superior for the 4DSF, with a median intensity decrease of -34 HU compared to -88 and -50 HU for the hepatic artery when using the TIPS filter and 4DSF + TIPS, respectively. The median peak-time accuracy was inferior for the 4DSF filter and 4DSF + TIPS, with a time shift of -1 phases for the portal vein TIC compared to no shift in time when using the TIPS. The analysis of the full-width-at-half-maximum (FWHM) of a small artery showed significantly less spatial resolution loss for the 4DSF at 3.2 pixels, compared to the TIPS filter at 4.3 pixels, and 3.4 pixels for the 4DSF + TIPS. The 4DSF can reduce noise with almost no resolution loss, making the filter very suitable for denoising 4D CT data for detection tasks, even in very low dose, i.e., very high noise level, situations. In combination with the TIPS filter, the noise reduction can be increased even further.

Detection of multiple abnormalities of breast cancer in mammograms using a deep dilated fully convolutional neural network

Breast cancer is one of the serious health issues which are quite frequent among women and the mortality rate due to it can be reduced if detected and diagnosed at the early stage. In this paper, a new generalized deep learning architecture, deep dilated fully convolutional neural network (DDFCNN), is designed to detect multiple abnormalities like mass, microcalcification, and architectural distortion in mammograms. The proposed DDFCNN architecture consists of a feature and a dilation module. In feature module, convolutional kernels of various sizes are used to capture information at different scales. Since there is no loss of resolution in the output of the dilation module, it renders more details which helps in localizing the anomaly properly. Several experiments are conducted on a widely used databases — DDSM and mini-MIAS where an accuracy of 95.33%, 91.42%, and 92.67% is measured for detection of mass, microcalcification, and architectural distortion, respectively with a FPs/I of 0.43, 0.50, and 0.46 for DDSM and similar assessment for mini-MIAS database are 96.56%, 89.63%, and 95.07% with a FPs/I of 0.29, 0.51, and 0.31. Moreover, the overall performance in detection of all the abnormalities in combination is 93.14% with 0.46 as FPs/I for DDSM whereas for mini-MIAS is 93.75% with 0.37 as FPs/I. The performance is further compared with the state-of-the-other methods where the proposed approach takes an edge.

A Space–Time Coding Array Sidelobe Optimization Method Combining Array Element Spatial Coding and Mismatched Filtering

Digital array radar (DAR) can fully realize digitalization at both the transmitting and receiving ends. However, the development of freedom at the transmitting end is far from mature. So, the new concept of multi-dimensional waveform coding array has appeared, which can optimize the transmitting resources in space–time/frequency waveform or another dimension. Space–time coding array (STCA) is a typical kind of multi-dimensional waveform coding array, which can make full use of the high degree of freedom at the transmitting end. It realizes emission diversity by introducing a small time delay between different transmission array elements. In this paper, an optimization method for STCA, which combines the array spatial coding at the transmitting end and mismatched filter design at the receiving end, is proposed. This method aims to solve the sidelobe problems of STCA: the inherent resonance phenomenon and the resolution loss problem. The experimental verification and quantitative comparative analysis prove the effectiveness of the proposed method. The resolution is restored to the ideal level under the premise of maintaining the beam-scanning ability and ultra-low sidelobe, and the resonance phenomenon caused by spectrum discontinuity is eliminated simultaneously.

A Novel Skip-Connection Strategy by Fusing Spatial and Channel Wise Features for Multi-Region Medical Image Segmentation.

Recent methods often introduce attention mechanisms into the skip connections of U-shaped networks to capture features. However, these methods usually overlook spatial information extraction in skip connections and exhibit inefficiency in capturing spatial and channel information. This issue prompts us to reevaluate the design of the skip-connection mechanism and propose a new deep-learning network called the Fusing Spatial and Channel Attention Network, abbreviated as FSCA-Net. FSCA-Net is a novel U-shaped network architecture that utilizes the Parallel Attention Transformer (PAT) to enhance the extraction of spatial and channel features in the skip-connection mechanism, further compensating for downsampling losses. We design the Cross-Attention Bridge Layer (CAB) to mitigate excessive feature and resolution loss when downsampling to the lowest level, ensuring meaningful information fusion during upsampling at the lowest level. Finally, we construct the Dual-Path Channel Attention (DPCA) module to guide channel and spatial information filtering for Transformer features, eliminating ambiguities with decoder features and better concatenating features with semantic inconsistencies between the Transformer and the U-Net decoder. FSCA-Net is designed explicitly for fine-grained segmentation tasks of multiple organs and regions. Our approach achieves over 48% reduction in FLOPs and over 32% reduction in parameters compared to the state-of-the-art method. Moreover, FSCA-Net outperforms existing segmentation methods on seven public datasets, demonstrating exceptional performance.

Ambiguous Losses and Their Traumatic Effects: A Qualitative Synthesis of the Research Literature

This paper presents a comprehensive review of the research literature on ambiguous losses, focusing on the psychological impact of uncertainty and the associated unresolved circumstances. The research method involves synthesizing data from various sources, including academic articles, books, and case studies, accessed through databases like PubMed, Scopus, Web of Science, and PsycINFO. The study underscores the need for specialized therapeutic methods and comprehensive support systems to improve the resilience and psychological health of those experiencing ambiguous loss. Effective coping strategies include seeking social support, engaging in meaning-making activities, and using therapeutic interventions like resilience training and narrative therapy to improve the psychological health of those experiencing ambiguous loss. Healthcare providers should integrate narrative therapy and resilience training techniques, while social workers should enhance support systems involving community networks. Further research is urgently advocated to expand the understanding of ambiguous loss across various contexts and cultures, leading to more effective, tailored interventions. The study's contributions are significant, as it elucidates the complex dynamics of ambiguous loss and identifies effective coping mechanisms and therapeutic interventions. By highlighting how ambiguous loss differs from other losses in closure and resolution, the paper offers new insights into the tailored approaches necessary to support affected individuals. The study consolidates diverse aspects of ambiguous losses and makes unique suggestions for policy and practice in one scholarly work.

Three-directional digital shearography based on SLM using a honeycomb-like pattern.

The possibility of utilizing a honeycomb-like modulation pattern in a spatial light modulator (SLM) for multi-directional synchronous digital shearography measurements has been investigated. Each modulation pixel in the honeycomb-like pattern modulates the shearing amount in different directions, thus avoiding the resolution loss associated with previous methods where a single pixel could only modulate a specific shearing direction. It was demonstrated that high-quality phase maps for different shearing directions can be simultaneously obtained with a single exposure.

Methods for constructing and evaluating consensus genomic interval sets.

The amount of genomic region data continues to increase. Integrating across diverse genomic region sets requires consensus regions, which enable comparing regions across experiments, but also by necessity lose precision in region definitions. We require methods to assess this loss of precision and build optimal consensus region sets. Here, we introduce the concept of flexible intervals and propose three novel methods for building consensus region sets, or universes: a coverage cutoff method, a likelihood method, and a Hidden Markov Model. We then propose three novel measures for evaluating how well a proposed universe fits a collection of region sets: a base-level overlap score, a region boundary distance score, and a likelihood score. We apply our methods and evaluation approaches to several collections of region sets and show how these methods can be used to evaluate fit of universes and build optimal universes. We describe scenarios where the common approach of merging regions to create consensus leads to undesirable outcomes and provide principled alternatives that provide interoperability of interval data while minimizing loss of resolution.

Assessing the 3D resolution of refocused correlation plenoptic images using a general-purpose image quality estimator

Correlation plenoptic imaging (CPI) is emerging as a promising approach to light-field imaging (LFI), a technique enabling simultaneous measurement of light intensity distribution and propagation direction from a scene. LFI allows single-shot 3D sampling, offering fast 3D reconstruction for a wide range of applications. However, the array of micro-lenses typically used in LFI to obtain 3D information limits image resolution, which rapidly declines with enhanced volumetric reconstruction capabilities. CPI addresses this limitation by decoupling the measurement of the light field on two photodetectors with spatial resolution, eliminating the need for micro-lenses. 3D information is encoded in a four-dimensional correlation function, which is decoded in post-processing to reconstruct images without the resolution loss seen in conventional LFI. This paper evaluates the tomographic performance of CPI, demonstrating that the refocusing reconstruction method provides axial sectioning capabilities comparable to conventional imaging systems. A general-purpose analytical approach based on image fidelity is proposed to quantitatively study axial and lateral resolution. The analysis fully characterizes the volumetric resolution of any CPI architecture, offering a comprehensive evaluation of its imaging performance.

Deep learning-aided respiratory motion compensation in PET/CT: addressing motion induced resolution loss, attenuation correction artifacts and PET-CT misalignment.

Respiratory motion (RM) significantly impacts image quality in thoracoabdominal PET/CT imaging. This study introduces a unified data-driven respiratory motion correction (uRMC) method, utilizing deep learning neural networks, to solve all the major issues caused by RM, i.e., PET resolution loss, attenuation correction artifacts, and PET-CT misalignment. In a retrospective study, 737 patients underwent [18F]FDG PET/CT scans using the uMI Panorama PET/CT scanner. Ninety-nine patients, who also had respiration monitoring device (VSM), formed the validation set. The remaining data of the 638 patients were used to train neural networks used in the uRMC. The uRMC primarily consists of three key components: (1) data-driven respiratory signal extraction, (2) attenuation map generation, and (3) PET-CT alignment. SUV metrics were calculated within 906 lesions for three approaches, i.e., data-driven uRMC (proposed), VSM-based uRMC, and OSEM without motion correction (NMC). RM magnitude of major organs were estimated. uRMC enhanced diagnostic capabilities by revealing previously undetected lesions, sharpening lesion contours, increasing SUV values, and improving PET-CT alignment. Compared to NMC, uRMC showed increases of 10% and 17% in SUVmax and SUVmean across 906 lesions. Sub-group analysis showed significant SUV increases in small and medium-sized lesions with uRMC. Minor differences were found between VSM-based and data-driven uRMC methods, with the SUVmax was found statistically marginal significant or insignificant between the two methods. The study observed varied motion amplitudes in major organs, typically ranging from 10 to 20mm. A data-driven solution for respiratory motion in PET/CT has been developed, validated and evaluated. To the best of our knowledge, this is the first unified solution that compensates for the motion blur within PET, the attenuation mismatch artifacts caused by PET-CT misalignment, and the misalignment between PET and CT.

Hybrid Aerosol Jet Printing and Electrodeposition of Ferrite-Sandwiched Inductors

The additive manufacturing (AM) of inductors of suitable size to be integrated into packaged electronics is attractive to customize the device characteristics for the specific application. Magnetic ferrite cores are widely used in conventional inductors, however, wire wound inductors can be brittle, heavy, bulky. Planar spiral inductors in a sandwich configuration present a promising alternative. Aerosol jet printing, a direct write AM technology, can deposit metal inks at a resolution of < 10 µm. Metal nanoparticle inks have limited conductivity at < 40% bulk. Building a higher profile may result in loss of resolution. Electrodeposition offers the ability to plate thick layers of highly conductive metals, including copper and nickel which are normally hard to access as nanoparticle inks compatible with aerosol jetting. We have previously shown the construction of a multi-layer flyback transformer with this hybrid aerosol jet and electrodeposition method. [1]We present a three-layer planar inductor with the first layer being a 1.3mm stack of ferrite sheets (Fair-Rite) with a 9 mm x 9 mm footprint integrated onto a circuit board. The intermediate layer consists of a spiral inductor made by aerosol jet printing UTDots Ag40X silver nanoparticle ink as the seed layer. To achieve high accuracy, the inductor was printed with a rotary axis stage. Electrodeposition in an acidic CuSO4 plating bath on top of the Ag seed layer resulted in thicknesses of 50 µm. A submicron layer of Ni is added for oxidation protection by electrodeposition from a Ni-Sulfamate bath. Lastly, a top 1.3 mm ferrite stack, with laser cut holes, is added to provide pass throughs for wire bonding. Figure 1 shows a micrograph of an inductor as printed, after electrodeposition, and with the top ferrite layer. Electrical measurements including impedance characteristics will be presented.SNL is managed and operated by NTESS under DOE NNSA contract DE-NA0003525. The SAND No. is SAND2023-14571A.Figure 1. Construction of a ferrite-sandwiched inductor with (a) the aerosol jet printing of an Ag seed layer, (b) after electroplating of Cu and Ni.

Surface fatigue crack segmentation in steel box girder bridges using an improved Unet convolutional neural network

Despite the success of crack segmentation, the traditional segmentation strategy and networks are not necessarily suitable for fatigue cracks with unique visual features. Towards the indistinct crack features, a network named Fatigue Crack Unet (FC-Unet) was proposed, where the novel split attention mechanism was incorporated to boost diverse feature extraction. Scaled by various ratios, raw images were cropped and constituted three datasets to represent different segmentation strategies. FC-Unet was trained and tested on each dataset, where the class imbalance problem was alleviated by the adjustment of loss functions. Results showed that the segmentation performance is mainly limited to noise interference. Preserving abundant global contexts to distinguish noises, the global inference is the superior strategy to the previous local inference. Leveraging the wide receptive field in global inference, the down-sampling rate can be decreased to save resolution losses. Combining the focus-based and region-based loss functions, the accuracy of imbalanced data was further improved. Compared with nine classical networks, the proposed FC-Unet enjoyed a powerful backbone and achieved a superior metric of 83.1% mean intersection over union (MIoU).

3D Correlation Imaging for Localized Phase Disturbance Mitigation

Correlation plenoptic imaging is a procedure to perform light-field imaging without spatial resolution loss, by measuring the second-order spatiotemporal correlations of light. We investigate the possibility of using correlation plenoptic imaging to mitigate the effect of a phase disturbance in the propagation from the object to the main lens. We assume that this detrimental effect, which can be due to a turbulent medium, is localized at a specific distance from the lens, and is slowly varying in time. The mitigation of turbulence effects has already fostered the development of both light-field imaging and correlation imaging procedures. Here, we aim to merge these aspects, proposing a correlation light-field imaging method to overcome the effects of slowly varying turbulence, without the loss of lateral resolution, typical of traditional plenoptic imaging devices.

Database size positively correlates with the loss of species-level taxonomic resolution for the 16S rRNA and other prokaryotic marker genes.

For decades, the 16S rRNA gene has been used to taxonomically classify prokaryotic species and to taxonomically profile microbial communities. However, the 16S rRNA gene has been criticized for being too conserved to differentiate between distinct species. We argue that the inability to differentiate between species is not a unique feature of the 16S rRNA gene. Rather, we observe the gradual loss of species-level resolution for other nearly-universal prokaryotic marker genes as the number of gene sequences increases in reference databases. This trend was strongly correlated with how represented a taxonomic group was in the database and indicates that, at the gene-level, the boundaries between many species might be fuzzy. Through our study, we argue that any approach that relies on a single marker to distinguish bacterial taxa is fraught even if some markers appear to be discriminative in current databases.

Evaluation of analytical columns suitable for high-temperature liquid-chromatography-isotope-ratio-mass-spectrometry analysis of anabolic steroids

Isotope ratio mass spectrometry (IRMS) can be used to determine the carbon isotope ratio of anabolic steroids. For example, in sports doping and food safety control, it enables determining an endogenous or synthetic origin of anabolic steroids. Generally, the steroids of interest are purified by liquid chromatography (LC) and analysed by gas chromatography combustion IRMS. LC-IRMS is not used since only mobile phases without carbon atoms can be used. For analysing mid-to apolar compounds, heated water can be used as an eluent as it has a similar polarity to a weak polar organic solvent. The silica-based columns are not robust enough at elevated temperatures in aqueous conditions. However, modified silica particles, metal oxides coated with polymers, and porous graphitic carbon are promising column materials for high-temperature LC (HT-LC) applications. Here, the stability of the stationary phase is crucial, and their chromatographic performance needs to be evaluated under the conditions mentioned above for anabolic steroid separations. Six columns using temperatures up to 200 °C were assessed, and only two were found to be appropriate. The ZirChrom-PBD column can be used for HT-LC-IRMS research purposes but is not recommended for routine laboratory practice applications due to the substantial loss of retention and resolution over time at elevated temperatures. Sachtopore-RP columns are the only suitable option for routine HT-LC-IRMS applications, even though they suffer from peak broadening over time when operating at elevated temperatures

Evaluating spectral performance for quantitative contrast-enhanced breast CT with a GaAs based photon counting detector: a simulation approach

Quantitative contrast-enhanced breast computed tomography (CT) has the potential to improve the diagnosis and management of breast cancer. Traditional CT methods using energy-integrated detectors and dual-exposure images with different incident spectra for material discrimination can increase patient radiation dose and be susceptible to motion artifacts and spectral resolution loss. Photon Counting Detectors (PCDs) offer a promising alternative approach, enabling acquisition of multiple energy levels in a single exposure and potentially better energy resolution. Gallium arsenide (GaAs) is particularly promising for breast PCD-CT due to its high quantum efficiency and reduction of fluorescence x-rays escaping the pixel within the breast imaging energy range. In this study, the spectral performance of a GaAs PCD for quantitative iodine contrast-enhanced breast CT was evaluated. A GaAs detector with a pixel size of 100 μm, a thickness of 500 μm was simulated. Simulations were performed using cylindrical phantoms of varying diameters (10 cm, 12 cm, and 16 cm) with different concentrations and locations of iodine inserts, using incident spectra of 50, 55, and 60 kVp with 2 mm of added aluminum filtration and and a mean glandular dose of 10 mGy. We accounted for the effects of beam hardening and energy detector response using TIGRE CT open-source software and the publicly available Photon Counting Toolkit (PcTK). Material-specific images of the breast phantom were produced using both projection and image-based material decomposition methods, and iodine component images were used to estimate iodine intake. Accuracy and precision of the proposed methods for estimating iodine concentration in breast CT images were assessed for different material decomposition methods, incident spectra, and breast phantom thicknesses. The results showed that both the beam hardening effect and imperfection in the detector response had a significant impact on performance in terms of Root Mean Squared Error (RMSE), precision, and accuracy of estimating iodine intake in the breast. Furthermore, the study demonstrated the effectiveness of both material decomposition methods in making accurate and precise iodine concentration predictions using a GaAs-based photon counting breast CT system, with better performance when applying the projection-based material decomposition approach. The study highlights the potential of GaAs-based photon counting breast CT systems as viable alternatives to traditional imaging methods in terms of material decomposition and iodine concentration estimation, and proposes phantoms and figures of merit to assess their performance.

Deep learning based bilateral filtering for edge-preserving denoising of respiratory-gated PET

BackgroundResidual image noise is substantial in positron emission tomography (PET) and one of the factors limiting lesion detection, quantification, and overall image quality. Thus, improving noise reduction remains of considerable interest. This is especially true for respiratory-gated PET investigations. The only broadly used approach for noise reduction in PET imaging has been the application of low-pass filters, usually Gaussians, which however leads to loss of spatial resolution and increased partial volume effects affecting detectability of small lesions and quantitative data evaluation. The bilateral filter (BF) — a locally adaptive image filter — allows to reduce image noise while preserving well defined object edges but manual optimization of the filter parameters for a given PET scan can be tedious and time-consuming, hampering its clinical use. In this work we have investigated to what extent a suitable deep learning based approach can resolve this issue by training a suitable network with the target of reproducing the results of manually adjusted case-specific bilateral filtering.MethodsAltogether, 69 respiratory-gated clinical PET/CT scans with three different tracers ([18F]\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$[^{18}\ ext {F}]$$\\end{document}FDG, [18F]\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$[^{18}\ ext {F}]$$\\end{document}L-DOPA, [68Ga]\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$[^{68}\ ext {Ga}]$$\\end{document}DOTATATE) were used for the present investigation. Prior to data processing, the gated data sets were split, resulting in a total of 552 single-gate image volumes. For each of these image volumes, four 3D ROIs were delineated: one ROI for image noise assessment and three ROIs for focal uptake (e.g. tumor lesions) measurements at different target/background contrast levels. An automated procedure was used to perform a brute force search of the two-dimensional BF parameter space for each data set to identify the “optimal” filter parameters to generate user-approved ground truth input data consisting of pairs of original and optimally BF filtered images. For reproducing the optimal BF filtering, we employed a modified 3D U-Net CNN incorporating residual learning principle. The network training and evaluation was performed using a 5-fold cross-validation scheme. The influence of filtering on lesion SUV quantification and image noise level was assessed by calculating absolute and fractional differences between the CNN, manual BF, or original (STD) data sets in the previously defined ROIs.ResultsThe automated procedure used for filter parameter determination chose adequate filter parameters for the majority of the data sets with only 19 patient data sets requiring manual tuning. Evaluation of the focal uptake ROIs revealed that CNN as well as BF based filtering essentially maintain the focal SUVmax\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\ ext {SUV}_\ ext {max}$$\\end{document} values of the unfiltered images with a low mean ± SD difference of δSUVmaxCNN,STD\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\delta \ ext {SUV}_\ ext {max}^{\ ext {CNN},\ ext {STD}}$$\\end{document} = (−3.9 ± 5.2)% and δSUVmaxBF,STD\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\delta \ ext {SUV}_\ ext {max}^{\ ext {BF},\ ext {STD}}$$\\end{document} = (−4.4 ± 5.3)%. Regarding relative performance of CNN versus BF, both methods lead to very similar SUVmax\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\ ext {SUV}_\ ext {max}$$\\end{document} values in the vast majority of cases with an overall average difference of δSUVmaxCNN,BF\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\delta \ ext {SUV}_\ ext {max}^{\ ext {CNN},\ ext {BF}}$$\\end{document} = (0.5 ± 4.8)%. Evaluation of the noise properties showed that CNN filtering mostly satisfactorily reproduces the noise level and characteristics of BF with δNoiseCNN,BF\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\delta \ ext {Noise}^{\ ext {CNN},\ ext {BF}}$$\\end{document} = (5.6 ± 10.5)%. No significant tracer dependent differences between CNN and BF were observed.ConclusionsOur results show that a neural network based denoising can reproduce the results of a case by case optimized BF in a fully automated way. Apart from rare cases it led to images of practically identical quality regarding noise level, edge preservation, and signal recovery. We believe such a network might proof especially useful in the context of improved motion correction of respiratory-gated PET studies but could also help to establish BF-equivalent edge-preserving CNN filtering in clinical PET since it obviates time consuming manual BF parameter tuning.

Multi-Scale Flow Estimation Within Stereoscopic PIV Processing Based On Deep Learning

Classical methods of particle-image velocimetry (PIV) based on state-of-the-art cross-correlation algorithms constitute a spatial filtering operation that leads to a loss of spatial resolution of the estimated velocity fields. This is associated with numerous limitations, e.g., when it comes to aligning high-fidelity numerical simulations, since the detection of small turbulent scales is often precluded. An optical flow neural network adapted for PIV processing, called RAFT-PIV, can overcome the drawback of reduced spatial resolution while maintaining the reliability and robustness of the classical approach. The present work aims to extend the application of the aforementioned deep learning based method by integrating it within a stereoscopic PIV processing workflow. Experimental data from an optically accessible single-cylinder combustion engine the flow field of which is characterized by complex, three-dimensional flow structures and a broad range turbulent scales are processed using RAFT-PIV. The 2D-3C vector fields obtained from stereoscopic reconstruction are compared against a classical cross-correlation based PIV evaluation, providing insight into instantaneous quantities and turbulent structures. A qualitative comparison of the instantaneous in-plane and out-of-plane velocity components reveals that the reconstructed flow obtained from the deep learning based estimation includes a wide range of resolved turbulent length scales. It outperforms the classical method. Furthermore, the findings of the present study demonstrate that the pixel accuracy has an effect on both physical quantities and turbulence characteristics. In particular, an increase in turbulent kinetic energy is observed across the engine cycles of the investigated engine flow states. Moreover, a higher fraction of three-dimensional isotropic turbulent structures is derived from RAFT-PIV compared to the results from the cross-correlation based technique.