With the rapid development of renewable energy, the penetration ratio of photovoltaic (PV) in the distribution network is getting higher and higher. The fair and reasonable allocation of high loss caused by high PV penetration in the distribution network is of great significance to the healthy development of distribution systems. In order to study the loss allocation in distribution networks with high PV penetration, based on the cooperative game theory, combined with the power flow algorithm, the power flow and loss of the 72-node active distribution network in agricultural and pastoral areas were analyzed. Three scenarios were included: no PV, low PV penetration, and high PV penetration. The allocated loss of the distribution network with high PV penetration and multiple distributed generators was computed. The results show that the loss allocation method, which takes into account the power as well as the network topology, is applicable to distribution networks with multiple distributed generators. In distribution networks with high PV penetration, distributed generators are the main cause of high loss. If the network contains the extreme reverse power flow concerned in this paper, the loss allocated to DGs accounts for more than 95% of the total network power loss.