Longissimus Dorsi Fat Research Articles

Effects of Roughage Quality and Particle Size on Rumen Parameters and Fatty Acid Profiles of Longissimus Dorsi Fat of Lambs Fed Complete Feed.

Simple SummaryRoughage type and particle size may play an important role in rumen volatile fatty acid levels and consequently affect fatty acid profile in meat and quality. Two roughage sources and two particles were used in this trial for growing lambs to study the effect in volatile fatty acids levels and fatty acids profile of longissimus dorsi (LD) fat. The results of this trial supported the hypothesis by confirming the effect of roughage type and particle size on meat quality and human health.The fatty acid composition for the longissimus dorsi (LD) fat of carcass sheep is a crucial factor impacting meat quality. We performed a 90-day feeding trial of 25 Naemi lambs to investigate the effects of roughage sources (alfalfa or wheat straw) of two sizes (regular and 1 cm chopped) when fed with pelleted total mixed ration (TMR) on the growth performance, fermentation patterns, and fatty acid (FA) composition of longissimus dorsi (LD) fat. Lambs were randomly assigned to individual pens with five treatment diets, as follows: C, control group with TMR; T1, TMR and regular alfalfa hay; T2, TMR and alfalfa hay chopped to 1 cm; T3, TMR and regular wheat straw; and T4, TMR and wheat straw chopped to 1 cm. Four lambs were randomly selected from each treatment (20 total) and sacrificed. LD fat of the carcass was extracted and analyzed for FA using a gas chromatography-mass spectrometry. Significantly increased feed intake was found in T1 and T2. The FA composition of LD fat in T2 had higher unsaturated fatty acid (UFA), omega-6 (n6), and omega-3 (n3) FA content. Conjugated linoleic acid (CLA) and α-linoleic acid were highest in lambs fed T1 and T2. Feeding different types of roughage, especially alfalfa hay, either regular or chopped, with total pelleted mixed ration is crucial to improving feed intake and body weight gain, as it positively enhances the rumen microbial fermentation process by controlling rumen pH. The FA profiles of meat from lambs fed TMR with regular or 1 cm particle size alfalfa hay (T1 and T2) are recommended for human consumption as a source of healthy FAs.

Evaluation of pigeon pea leaves (Cajanus cajan) replacing alfalfa meal on growth performance, carcass trait, nutrient digestibility, antioxidant capacity and biochemical parameters of rabbits.

A 30-day experiment was performed to determine the effect of pigeon pea leaves (PPL) on growth performance, carcass trait, meat quality, nutrient digestibility, antioxidant capacity and biochemical parameters of growing rabbits. In a completely randomized design, PPL replaced alfalfa meal at the level of 0%, 10%, 20% and 30%, which were named PPL0 (control), PPL10, PPL20 and PML30 respectively. Two hundred New Zealand white rabbits at 6weeks with similar weight (870.23±15.98g) were allocated to four dietary groups with five replicates containing 10 rabbits/per replicate (male). The results showed that: (a) PPL powder contained 24.26% crude protein, 4.34% crude fat, 17.86% crude fibre, 7.05% ash, 1.35% calcium, 0.28% phosphorus, 1.09% lysine and 0.20% methionine, and the chemical compositions are on DM basis; (b) the ratio of feed to gain of rabbits fed diet PPL10 was significantly better (p<0.05) than those fed other three diets; (c) the content of longissimus dorsi (LD) moisture in the rabbits fed diets without PPL (control group) was 12% lower than that in the PPL30 diets (60.1 vs. 72.1; p<0.05). In PPL10, PPL20 and PPL30 diets, the leg muscle (LM) b*(yellowness) value was 33%, 30% and 22.6% higher than the control group respectively. The rabbits fed diets PPL0 had lower (p<0.05) LM crude protein and ash and higher (p<0.05) crude fat of LD and LM as compared with those fed other diets; (d) crude protein and energy digestibility of PPL0 and PPL10 diets were significantly higher (p<0.05) than PPL30 diets; and (e) serum glutathione peroxidase (GSH-Px) activity of the rabbits fed PPL10 and PPL30 diets was significantly higher (p<0.05) than that fed PPL20 diets. Liver total antioxidant capacity (T-AOC) activity of the PPL30 groups was 1.3% higher (p<0.05) than the PPL10 group. Additionally, the control group (PPL0) had the highest (p<0.05) blood urea nitrogen (BUN), total cholesterol (TCHO) and low-density lipoprotein cholesterol (LDLC) content compared with the groups supplemented with PPL. The PPL30 group had the highest (p<0.05) triiodothyronine (T3 ) and tetraiodothyroxine (T4 ) value among the dietary groups.

Perfil de ácidos graxos e características da carne de bovinos Nelore confinados com diferentes fontes lipídicas protegidas

O objetivo deste trabalho foi avaliar os efeitos da adição de fontes de lipídeos naturais e protegidos da degradação ruminal na dieta de bovinos Nelore confinados sobre características da carne, concentrações de lipoproteínas sanguíneas e perfil de ácidos graxos da gordura do músculo Longissimus dorsi. Foram utilizados 120 bovinos Nelore, não castrados (366,9±28,7kg), com aproximadamente 24 meses. O delineamento experimental foi inteiramente casualizado, com três tratamentos: (CONTR) sem fonte adicional de lipídeo, (GDESP) com fonte de lipídeo natural (torta de algodão) e (GPROT) com fonte de lipídeo protegido rico em ácidos graxos poli-insaturados. Foram utilizadas 24 baias, cinco animais/baia, com oito repetições/tratamento, sendo as baias consideradas unidades experimentais. Foi observada (P&lt;0,05) interação entre tratamento e dias de mensuração para colesterol e LDL, com maiores valores do tratamento GPROT nos dias 1 e 15, tendo o dia 84 valor intermediário aos demais tratamentos. Observaram-se efeitos de dias de mensuração, em que VLDL e triglicerídeos apresentaram valores crescentes nos dias 15, 84 e 1; enquanto o HDL teve maior valor no dia 1. Foi observado efeito (P&lt;0,05) de tratamento para o perfil de ácidos graxos, com diminuição do C14:1, C16:1 e C17:1 e aumento do ácido transvaccênico, C18:2, ácidos graxos poli-insaturados (AGPI) e da relação AGPI: AGMI (ácidos graxos monoinsaturados) para os tratamentos GPROT e GDESP. Adição de lipídios na dieta, independentemente da fonte, promove melhora na composição de ácidos graxos da carne de bovinos Nelore confinados, aumentando a quantidade de ácidos graxos insaturados sem alterar as características qualitativas da carne.

Effects of live weight at slaughter on fatty acid composition of Longissimus dorsi and Biceps femoris muscles of indigenous Lori goat.

This study aimed to determine fatty acid (FA) composition of Longissimus dorsi (LD) and Biceps femoris (BF) muscles of an Iranian indigenous goat (Lori goat) at two live weights at slaughter (LWS). Twenty male Lori goats (5 to 8 months) raised in nomadic system were slaughtered either at LWS less than 20 kg (light) or LWS more than 30 kg (heavy). Carcass dressing and FA composition of intramuscular fat of LD and BF muscles as well as cholesterol content of LD muscle were determined. Heavy goats had higher dressing percentage than light ones (42.7vs.39.3%, P < 0.01). The predominant n-6 FA were C18:2, and C20:4 while C22:5, C20:5, C18:3, C20:3, and C22:6 were the n-3 FA detected. Polyunsaturated and saturated FA contributed 22% and 36% of the total FA in both muscles, respectively. Palmitic acid (C16:0) of LD was higher in heavy compared to the light goats (P < 0.05). BF muscle had higher α-linolenic acid (18:3 n-3) as percentage than LD muscle (P < 0.05). The ratio of n-6/n-3 FA and polyunsaturated/saturated FA were 3.8 and 0.6, respectively. Cholesterol content of LD muscle of light and heavy goats were 71.2 ± 16 and 59.5 ± 14 mg per 100 g fresh meat respectively. In conclusion, desirable PUFA/SFA (0.6) and n-3/n-6 ratio (3.8) found in indigenous Lori goat propose healthy source of lean meat for the consumers.

Effect of different roe deer muscles on fatty acid composition in intramuscular fat

Abstract The aim of the work was to study the effect of different muscles on the fatty acid composition in the intramuscular fat of roe deer hunted in Lithuania. The samples were excised from the five muscles of different carcass sites: m. longissimus dorsi (LD), m. deltoideus (shoulder), m. tensor fascia e latae (hind quarter), m. cleidocipitalis (neck) and m. intercostales interni (brisket) of hunted animals. The data were subjected to the analysis of variance in general linear (GLM Multivariate) procedure in SPSS 17. The muscle location of roe deer males appeared to affect the fatty acid composition in the intramuscular fat. The total proportions of saturated (SFA ), monounsaturated (MUFA ) and polyunsaturated (PUFA), including individual SFA, MUFA and PUFA acids were affected by the muscle. The highest levels of SFA and MUFA and the lowest levels of PUFA were found in the intramuscular fat of neck and brisket muscles and vice versa, the lowest levels of SFA and MUFA and the highest levels of PUFA were found in the intramuscular fat of LD and hind quarter muscles. The muscle type of roe deer appeared to affect the lipid quality indices. The lowest atherogenic and thrombogenic indexes, and the highest hypocholesterolemic/hypercholesterolemic ratio were in the intramuscular fat of LD and hind quarter muscles.

Fatty acid composition differences between adipose depot sites in dairy and beef steer breeds

The objective of the study was to compare fatty acid composition of longissimus dorsi (LD) and kidney fat (KF) in Holstein steers (HS), Simmental steers (SS) and Chinese LongDong Yellow Cattle steers (CLD). All steers received the same nutrition and management but in different locations. Cattle were harvested at approximately 550kg and fatty acid composition of longissimus dorsi and kidney fat was analyzed in samples taken after 3days of aging. There was evidence (P < 0.05) that C18:3n6 was greater in KF than LD in CLD cattle but not in HS or SS cattle. Percentage C18:1n9, C18:2n6, C18:3n3, and n6 fatty acids were greater in LD than KF for all breeds (P < 0.05), but the difference between fat sources for n6 in CLD cattle was smaller than the other two breeds. The LD had greater percentage of polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA), and a greater ratio of n6:n3 PUFAs compared to the KF in each breed (P < 0.05). The △(9)-desaturase catalytic activity index was greater in LD than in KF in each breed group (P < 0.05). Percentage cis-9, trans-11 CLA was greater in KF than LD in HS (P < 0.05) but not SS or CLD cattle. These results indicate fatty acid percentages generally differed between longissimus dorsi fat and kidney fat. Further, there was some indication that some of these differences between fatty acid deposition sites were not consistent across breed group.

Chemical structure of european bison musculus longissimus dorsi at different stages of age

The aim of this study was to investigate the chemical composition of musculus longissimus dorsi muscle in European bison (Bison bonasus) of the age 6, 9, 12 and 14 years. In m. longissimus dorsi water content was from 74.90 g (group until 6 years of age) until 75.70 g.100 g-1 (group until 12 years of age). Non statistically significant differences (P≥0.05) were found between groups of age. In m. longissimus dorsi the protein content was statistically significant during aging (P≥0.05) of the European bison from 21.23 (group until 12 years of age) until 22.34 g.100 g-1 (group until 14 years of age). The protein content is comparable with the values of steers and bulls of different breeds of cattle feedlot and meat buffalo. The m. longissimus dorsi fat content of European bison was represented from 1.26 g (group until 12 years of age) to 2.11 g.100 g-1 (group until 9 years of age), without statistical differences (P≥0.05) between groups of age. Fat levels are comparable with American bison fat levels and European bison meat from this perspective be regarded as high dietary, maybe. Tendency increasing of fat content in muscle with increasing age of animals was not confirmed (P≥0.05) but was confirmed that this variable indicator has the greatest potential impact nutrition. Energy value in 100 g m. longissimus dorsi was from 402.81 kJ (group until 12 years of age) to 447.07 kJ.100 g-1 (group to 9 years of age). The energy value in 100 g muscle was recorded only statistical differences (P≤0.05) in the group 9 and 12 years of age. Experiment results confirmed that the European bison meat is good article and possible supplement in the diet and the human food chain especially in states where the farm is kept in a manner respectively, as a delicacy, because it contains low representation of fat, what ultimately increases its particular dietary value, moving it from this perspective, even before the beef meat.

Effects of feed supplementation of zilpaterol hydrochloride on growth performance and carcass traits of finishing lambs

Effects of zilpaterol hydrochloride (ZH) on growth performance trial (30-day period), and carcass traits of finishing lambs were evaluated. Twenty-eight male Rambouillet lambs (32.7±2.6 kg body weight) were randomly assigned to diets with: 0, 5.3, 10.6, and 15.9 mg ZH/kg dry matter (DM). Average daily weight gain, hot and chilled carcass dressing, muscle conformation, hindquarters width, major and minor torax width, crude protein, and ash of Longissimus dorsi (LD) increased linearly as ZH level increased. Feed conversion was linearly improved, and perirenal fat, leg length, and fat of LD were linearly reduced as ZH level increased. Hot and chilled carcass dressing, dorsal fat, area, and sherar forces values of LD, were cubically affected by ZH level, which at 15.9 mg/kg DM offered the best values. The ZH supplementations at 15.9 mg/kg DM showed positive effects on growth performance and carcass traits.

Fatty acid composition of lamb meat as affected by production system, weaning and sex

Fatty acid composition in intramuscular fat of Longissimus dorsi (LD) of 32 Jezersko-Solčava lambs was measured in two kinds of production system (grazing on mountain pasture and fattening in stable with hay and cereals ad libitum) and two kinds of weaning system (suckling and weaned) and interaction between them. The effect of sex was also determined. Pasture and stable lambs were slaughtered at average live weight 34 kg. Suckling lambs were slaughtered at 125 days of age (30 kg of live weight) and weaned lambs were slaughtered at 165 days (38 kg of live weight). Lambs on pasture displayed lower percentage of palmitic (C16:0) and oleic (C18:1n-9) and higher percentage of linoleic (C18:2n-6), α-linolenic (C18:3n-3), arachidonic (C20:4n-6) and eicosapentaenoic (C20:5n-3) fatty acids. Pasture lambs had lower percentage of SFA (saturated fatty acids) and MUFA (monounsaturated fatty acids), higher percentage of PUFA (polyunsaturated fatty acids) and especially relatively high percentage of C18:3n-3 (3.3%) in intramuscular fat. Pasture lambs also had higher percentage of n-6 PUFA (15.06%), even 1.5 times higher than stable lambs (9.34%) and consecutively higher n-6/n-3 PUFA ratio (2.1 vs. 1.6). Above all weaning affected MUFA and PUFA. Intramuscular fat of suckling lambs contained less MUFA (34.84% vs. 38.35%) and more PUFA (21.89% vs. 17.08%) as well as higher n-6/n-3 ratio than weaned lambs (1.9 vs. 1.7). The interaction between production system and weaning was statistically significant only for α-linolenic acid, with the highest proportion in LD fat of weaned lambs grazing on pasture (3.48%) and the lowest proportion of weaned lambs fatten in stable (2.71%). Males had lower values of MUFA and higher values of PUFA, n-6 PUFA and n-3 PUFA. The n-6/n-3 PUFA ratio was the same in both sexes (1.8).

Effects of a compensatory growth strategy on sensory and physical properties of meat from young bulls

The objective of the present study was to investigate the sensory properties, with special emphasis on tenderness, of meat from strategically fed young bulls (13 months of age) slaughtered when a plateau in protein turnover was observed. Twelve Holstein Friesian young bulls were divided into two feeding strategies. One group of young bulls ( n = 6) were fed ad libitum throughout the rearing period (AD) whereas the second group ( n = 6) was subjected to a compensatory growth feeding strategy (CO). Sensory profiling of beef longissimus dorsi (LD), semimembranosus (SM) and supraspinatus (SS) was performed in addition to physical measurements (shear force) and content of intramuscular fat of LD. The data was analysed using a regression-based multivariate data analytical strategy. In relation to predictivity of the various shear force measurement constituents for sensory texture, it was determined that a number of the responses collected (e.g. max mm), in addition to the commonly used max (N) may be utilised to predict subtlety in the sensory texture differences of the samples (e.g. Crumbliness) with respect to compensatory feeding. Through profiling LD and SS were found to have enhanced texture and flavour properties when the young bulls were fed ad libitum during rearing. However, the SM samples were found to improve in characteristics regarding texture and appearance when the young bulls had been fed compensatorily. Thus, compensatory feeding as a texture improvement strategy proved to be highly dependant on muscle type. Of note, LD and SS were found to develop an off-flavour designated as ‘steer taint’ when derived from compensatory feeding. This was postulated as potentially not a problem for the consumer, in LD as the level was not significant, and in SS due to sensory masking when commonly prepared as a stew or casserole. Considering the different preparation methods used for meat from LD, SM and SS, compensatory feeding may be considered to have improved the texture and elevated the eating quality where it was most relevant, namely in SM roasts.