Longer Pulse Width Research Articles

Tuning the Locally Enhanced Electric Field Treatment (LEEFT) between Electrophysical and Electrochemical Mechanisms for Bacteria Inactivation.

Efficient drinking water disinfection methods are critical for public health. Locally enhanced electric field treatment (LEEFT) is an antimicrobial method that uses sharp structures, like metallic nanowires, to enhance the electric field at tips and cause bacteria inactivation. Electroporation is the originally designed mechanism of LEEFT. Although oxidation is typically undesired due to byproduct generation and electrode corrosion, it can enhance the overall disinfection efficiency. In this work, we conduct an operando investigation of LEEFT, in which we change the electrical parameters to tune the mechanisms between electrophysical electroporation and electrochemical oxidation. Pure electroporation (i.e., without detectable oxidation) could be achieved under a duty cycle of ≤0.1% and a pulse width of ≤2 μs. Applying 2 μs pulses at 7-8 kV/cm and 0.1% duty cycle results in 80-100% bacteria inactivation with pure electroporation. A higher chance of oxidation is found with a higher duty cycle and a longer pulse width, where the antimicrobial efficiency could also be enhanced. For water with a higher conductivity, a higher antimicrobial efficiency can be achieved under the same treatment conditions, and electrochemical reactions could be induced more easily. The findings shown in this work improve the fundamental understanding of LEEFT and help optimize the performance of LEEFT in real applications.

Numerical Investigation on the Effect of Electrical Parameters on the Discharge Characteristics of NS-SDBD

There are numerous scientific and engineering fields where the surface dielectric barrier discharge driven by nanosecond pulses (NS-SDBD) has important applications. To improve its performance, more research is still needed on the effects of electrical parameters on the NS-SDBD actuator’s discharge characteristics. In this study, a two-dimensional numerical model based on 13 discharge particle chemical processes was constructed using a numerical simulation approach, producing findings for the NS-SDBD actuator’s voltage–current (V-A) characteristics, discharge profile, and spectrum analysis. Additionally, a comprehensive investigation into the trends and underlying mechanisms of the effects of the voltage amplitude, pulse width, rise time, and fall time parameters on the discharge behavior of the NS-SDBD actuator was carried out. The results show that higher voltage amplitudes increase the maximum current and electron density, which enhances the plasma excitation effect. The peak power deposition during the second discharge is also raised by longer pulse widths and rise times, whereas the total power deposition during the second discharge is decreased by longer fall times.

Experimental study on the influence of thermal accumulation on breakdown in slow-wave structures

Radio frequency (RF) breakdown in the slow-wave structures (SWSs) is a crucial bottleneck restricting relativistic backward wave oscillator (RBWO) to pursuing higher output power and longer pulse width. This paper has experimentally studied the influence of thermal accumulation during repetitive operation on RF breakdown in an X-band RBWO. A method for cooling the SWSs using water flow has been proposed to restrain the temperature rise to some extent. Under different heat dissipation conditions, the operating states of the RBWOs exhibit great differences. The greater the distance between the water-cooling heat transfer channel and the SWSs, the more serious the pulse shortening of high-power microwave. Moreover, the breakdown traces appearing in the SWSs become more obvious with the worse convective heat transfer capacity. The observed experimental phenomena provide a new guideline that helps to enrich the mechanism of RF breakdown and to explore corresponding suppression methods in RBWO.

Advancements of the nSpec system

A new characterization of the detector response for the EJ301 liquid scintillator based nSpec system has been conducted at the Los Alamos Neutron Science Center. The new characterization was accomplished in two orders of magnitude less time and with better statistics than the previous characterization. The new and old characterization methods were compared by conducting both types of characterization on two detectors (one EJ301 and one EJ301D). The two methods show consistent results and compare well to results previously published in the literature; however, the results from the characterization of the original nSpec detector show discrepancies that have been identified as being caused by oxygen being absorbed into the detector due to a leak. The new characterized detector response shows improved spectrum unfolding performance than the previous characterization. The cross talk of the detector system was also characterized as a function of incident neutron energy and found to have a negligible impact on spectrum unfolding results.Alternative materials (EJ301D and stilbene) were also characterized in a first step towards an upgraded system. The different materials show little difference in unfolding performance, but the comparison is ongoing. A SiPM based stilbene detector was used to evaluate the use of SiPMs in a future system upgrade. The SiPM provides adequate performance, but the custom SiPM readout results in a much longer pulse width than PMT based systems. The measured light output of the SiPM based stilbene compares well with the results in literature. Future work will look at additional materials (deuterated stilbene and organic glass) and optimizing the SiPM readout to decrease the pulse width.

Interaction Energy Dependency on Pulse Width in ns NIR Laser Scanning of Silicon.

Laser ablation of semiconductor silicon has been extensively studied in the past few decades. In the ultrashort pulse domain, whether in the fs scale or ps scale, the pulse energy fluence threshold in the ablation of silicon is strongly dependent on the pulse width. However, in the ns pulse scale, the energy fluence threshold dependence on the pulse width is not well understood. This study elucidates the interaction energy dependency on pulse width in ns NIR laser ablation of silicon. The level of ablation or melting was determined by the pulse energy deposition rate, which was proportional to laser peak power. Shorter pulse widths with high peak power were likely to induce surface ablation, while longer pulse widths were likely to induce surface melting. The ablation threshold increased from 5.63 to 24.84 J/cm2 as the pulse width increased from 26 to 500 ns. The melting threshold increased from 3.33 to 5.76 J/cm2 as the pulse width increased from 26 to 200 ns, and then remained constant until 500 ns, the longest width investigated. Distinct from a shorter pulse width, a longer pulse width did not require a higher power level for inducing surface melting, as surface melting can be induced at a lower power with the longer heating time of a longer pulse width. The line width from surface melting was less than the focused spot size; the line appeared either as a continuous line at slow scanning speed or as isolated dots at high scanning speed. In contrast, the line width from ablation significantly exceeded the focused spot size.

MScanFit motor unit number estimation of abductor pollicis brevis: Findings from different experimental parameters.

MScanFit motor unit number estimation (MUNE) based on the recording of the compound muscle action potential (CMAP) scan has wide applications. This study evaluated the effect of different CMAP scan settings on MScanFit MUNE. CMAP scan of the abductor pollicis brevis (APB) muscle was performed in 10 healthy subjects at a United States (US) research center using different stimulus pulse widths (0.1, 0.2 ms) and total number of stimuli or steps (500, 1,000), and in 12 healthy subjects at a China research center using a 0.1 ms pulse width and 500 steps. MScanFit MUNE was derived using the default model parameters. A significantly higher MUNE was obtained using the shorter than longer pulse width; 84.70 ± 21.56 (500 steps) and 77.90 ± 27.62 (1,000 steps) at a pulse width of 0.1 ms vs. 67.60 ± 18.72 (500 steps) and 62.20 ± 15.82 (1,000 steps) at a pulse width of 0.2 ms (p < 0.05). However, MUNE was unrelated to the number of steps (500 vs. 1,000, p > 0.1). MUNE was significantly higher in persons studied in the China center (136.42 ± 32.46) than the US center (84.70 ± 21.56) despite each center using the same pulse widths and steps (p < 0.001). After excluding the ethnicity, age and experimenter factors, this significant difference is speculated to be partly related to different electrode size used in the two centers. The findings suggest that CMAP scan experimental parameters should remain consistent, so the MScanFit MUNE will not be compromised by non-physiological factors.

Investigation of the effects of pulse width modulation on the laser sintering of LATP for all-solid-state batteries

Inorganic solid electrolytes are the most important component for realizing all-solid-state batteries with lithium metal anodes and enable safe battery cells with high energy densities. Their synthesis and processing are the subject of current research, especially the NASICON-type Li1+xAlxTi2-x(PO4)3 (LATP). Herein, the ability of sintering with electro-magnetic irradiation is investigated and correlated with different properties of prepared LATP pellets. First of all, an infrared camera records the temperature of the surface during the treatment. Second, the effect of the pulse fluence is investigated in terms of the topology and morphology of the pellets. Here, the arithmetic surface roughness Ra is the main parameter. Then, the depth of the radiation interaction in the pellet is measured. The focus of this paper is on the different pulse widths of the laser sources, and therefore, similar pulse and hatch overlap ensure equivalent areal energy input in both cases. As a summarized result, treatment with a shorter pulse width generates high peak pulse powers, resulting in higher temperatures, rougher surfaces and affecting deeper layers of the pellets compared to treatment with longer pulse width. On the contrary, excessive power leads to the ablation of the material up to destruction.

The evoked compound nerve action potential is shaped by the electrical pulse-width

Abstract Introduction: Despite its central role in medicine electrical stimulation (ES) is still limited by its selectivity. Different reports did assess effects of different waveforms, intensities, and frequency on the activation threshold of nerve fibres with different diameters. We aimed to extend this knowledge by investigating the effect of short monophasic rectangular pulses (1, 2, 5, 10, 50, 100 and 200 μs) on the recruitment order. Methods:The sciatic nerve of rats was stimulated, and the evoked compound nerve action potential (CNAP) measured at two sites on the tibialis nerve, using epineural electrodes. Changes in delay, amplitude, and the shape of the CNAP were analyzed. Results:The amplitude and delay of the CNAP were significantly affected by the pulse-width (PW). The delay and duration of the compound nerve action potential increased with longer PW, while the amplitude decreased. Discussion:Found changes are likely caused by changes in the time point of excitation of individual neuron fibres, depending on electrical field strength and exposure time. This might be of particular interest when selecting PWs for design and validation of stimulation patterns and analysis of experimental and clinical observations.

Generation of Tumor-activated T cells Using Electroporation

Expansion of cytotoxic T lymphocytes (CTLs) is a crucial step in almost all cancer immunotherapeutic methods. Current techniques for expansion of tumor-reactive CTLs present major limitations. This study introduces a novel method to effectively produce and expand tumor-activated CTLs using high-voltage pulsed electric fields. We hypothesize that utilizing high-voltage pulsed electric fields may be an ideal method to activate and expand CTLs due to their non-thermal celldeath mechanism.Tumor cells were subjected to high-frequency irreversible electroporation (HFIRE) with various electric field magnitudes (1250, 2500 V/cm) and pulse widths (1, 5, and 10 µs), or irreversible electroporation (IRE) at 1250 V/cm. The treated tumor cells were subsequently cocultured with CD4+ and CD8+ T cells along with antigen-presenting cells.We show that tumor-activated CTLs can be produced and expanded when exposed to treated tumor cells. Our results suggest that CTLs are more effectively expanded when pulsed with HFIRE conditions that induce significant cell death (longer pulse widths and higher voltages). Activated CD8+ T cells demonstrate cytotoxicity to untreated tumor cells suggesting effector function of the activated CTLs. The activated CTLs produced with our technique could be used for clinical applications with the goal of targeting and eliminating the tumor.

Optical power limiter in the femtosecond filamentation regime

We present the use of a power limiting apparatus to evaluate ultrafast optical nonlinearities of transparent liquids (water and ethanol) in the femtosecond filamentation regime. The setup has been previously employed for the same purpose, however, in a longer pulsewidth (> 20 ps) regime, which leads to an ambiguous evaluation of the critical power for self-focusing. The uncertainty originates from the existence of a threshold power for optical breakdown well below the critical power for self-focusing within this timeframe. Contrarily, using the proposed apparatus in the femtosecond regime, we observe for the first time a unique optical response, which features the underlying physics of laser filamentation. Importantly, we demonstrate a dependence of the optical transmission of the power limiter on its geometrical, imaging characteristics and the conditions under which a distinct demarcation for the critical power for self-focusing can be determined. The result is supported by numerical simulations, which indicate that the features of the observed power-dependent optical response of the power limiting setup are physically related to the spontaneous transformation of the laser pulses into nonlinear conical waves.

Two-photon calcium imaging of neuronal and astrocytic responses: the influence of electrical stimulus parameters and calcium signaling mechanisms

Objective. Electrical brain stimulation has been used to ameliorate symptoms associated with neurologic and psychiatric disorders. The astrocytic activation and its interaction with neurons may contribute to the therapeutic effects of electrical stimulation. However, how the astrocytic activity is affected by electrical stimulation and its calcium signaling mechanisms remain largely unknown. This study is to explore the influence of electrical stimulus parameters on cellular calcium responses and corresponding calcium signaling mechanisms, with a focus on the heretofore largely overlooked astrocytes. Approach. Using in vivo two-photon microscopy in mouse somatosensory cortex, the calcium activity in neurons and astrocytes were recorded. Main results. The cathodal stimulation evoked larger responses in both neurons and astrocytes than anodal stimulation. Both neuronal and astrocytic response profiles exhibited the unimodal frequency dependency, the astrocytes prefer higher frequency stimulation than neurons. Astrocytes need longer pulse width and higher current intensity than neurons to activate. Compared to neurons, the astrocytes were not capable of keeping sustained calcium elevation during prolonged electrical stimulation. The neuronal Ca2+ influx involves postsynaptic effects and direct depolarization. The Ca2+ surge of astrocytes has a neuronal origin, the noradrenergic and glutamatergic signaling act synergistically to induce astrocytic activity. Significance. The astrocytic activity can be regulated by manipulating stimulus parameters and its calcium activation should be fully considered when interpreting the mechanisms of action of electrical neuromodulation. This study brings considerable benefits in the application of electrical stimulation and provides useful insights into cortical signal transduction, which contributes to the understanding of mechanisms underlying the therapeutic efficacy of electrical stimulation for neurorehabilitation applications.

The Effect of Clinically Controllable Factors on Neural Activation During Dorsal Root Ganglion Stimulation

ObjectiveDorsal root ganglion stimulation (DRGS) is an effective therapy for chronic pain, though its mechanisms of action are unknown. Currently, we do not understand how clinically controllable parameters (e.g., electrode position, stimulus pulse width) affect the direct neural response to DRGS. Therefore, the goal of this study was to utilize a computational modeling approach to characterize how varying clinically controllable parameters changed neural activation profiles during DRGS. Materials and MethodsWe coupled a finite element model of a human L5 DRG to multicompartment models of primary sensory neurons (i.e., Aα-, Aβ-, Aδ-, and C-neurons). We calculated the stimulation amplitudes necessary to elicit one or more action potentials in each neuron, and examined how neural activation profiles were affected by varying clinically controllable parameters. ResultsIn general, DRGS predominantly activated large myelinated Aα- and Aβ-neurons. Shifting the electrode more than 2 mm away from the ganglion abolished most DRGS-induced neural activation. Increasing the stimulus pulse width to 500 μs or greater increased the number of activated Aδ-neurons, while shorter pulse widths typically only activated Aα- and Aβ-neurons. Placing a cathode near a nerve root, or an anode near the ganglion body, maximized Aβ-mechanoreceptor activation. Guarded active contact configurations did not activate more Aβ-mechanoreceptors than conventional bipolar configurations. ConclusionsOur results suggest that DRGS applied with stimulation parameters within typical clinical ranges predominantly activates Aβ-mechanoreceptors. In general, varying clinically controllable parameters affects the number of Aβ-mechanoreceptors activated, although longer pulse widths can increase Aδ-neuron activation. Our data support several Neuromodulation Appropriateness Consensus Committee guidelines on the clinical implementation of DRGS.

Role of temperature, MTJ size and pulse-width on STT-MRAM bit-error rate and backhopping

The switching voltage (Vc) variability of STT-MRAM has been investigated with respect to the effects of temperature, magnetic tunnel junction (MTJ) diameter, bias pulse-width (PW) and backhopping (BH). Arrays of STT-MRAM devices having 1 transistor + 1 MTJ bitcell structure with ±10 nm MTJ diameters were tested under an applied ramped voltage waveform sweep at temperatures ranging from −10 °C to 125 °C with pulsewidths of 40 ns to 10 µs. We show that the Vc distributions as a function of temperature and PW are highly skewed. BH of the MTJ bits increases with increasing temperature, longer PW and smaller MTJ diameter. We also demonstrate the switching and BH performances of 40 Mb embedded MRAM macro having different MTJ diameters.

Generating artificial sensations with spinal cord stimulation in primates and rodents

For patients who have lost sensory function due to a neurological injury such as spinal cord injury (SCI), stroke, or amputation, spinal cord stimulation (SCS) may provide a mechanism for restoring somatic sensations via an intuitive, non-visual pathway. Inspired by this vision, here we trained rhesus monkeys and rats to detect and discriminate patterns of epidural SCS. Thereafter, we constructed psychometric curves describing the relationship between different SCS parameters and the animal's ability to detect SCS and/or changes in its characteristics. We found that the stimulus detection threshold decreased with higher frequency, longer pulse-width, and increasing duration of SCS. Moreover, we found that monkeys were able to discriminate temporally- and spatially-varying patterns (i.e. variations in frequency and location) of SCS delivered through multiple electrodes. Additionally, sensory discrimination of SCS-induced sensations in rats obeyed Weber's law of just-noticeable differences. These findings suggest that by varying SCS intensity, temporal pattern, and location different sensory experiences can be evoked. As such, we posit that SCS can provide intuitive sensory feedback in neuroprosthetic devices.

Application of Pulsed Electric Field for Inactivation of Yeast S. cerevisiae in Apple Juice

Pulsed electric filed (PEF) is a non-thermal food preservation method as alternative to traditional thermal method. The effect of pulsed electric field (PEF) on inactivation of yeast Saccharomyces cerevisiae (S.cerevisiae) in apple juice containing different sugar concentration was studied. The results of this study have shown that, using PEF, the yeast could be inactivated at room temperature (20°C). The inactivation effect of PEF was dependent on pulse number, field strength, and pulse width as well as sugar concentration. Pulse numbers less than 20 pulses (at 12.6kV/cm) had no or very less effect on yeast inactivation. Increasing the pulse number up to 400 pulses resulted about 3 logs yeast inactivation. The most important factor for yeast inactivation was the field strength (10 to 30 kV/cm). Whereas, at 10.2 kV/cm and 100 pulses, less than 2 logs yeast inactivation could be achieved, was the yeast inactivation at filed strength of 30kV/cm and 100 pulses about 5 logs. At given field strength and pulse number, longer pulse width (1 to 2.5 μF) affected positively the inactivation of microorganism. In contrast, increasing the sugar concentration in apple juice higher than 20% negatively affected the inactivation of yeast during PEF treatment at given treatment conditions.

Atomic scale microscopy unveils the growth mechanism of 2D-like CuO nanoparticle agglomerates produced via electrical discharges in water

In this paper we describe the growth mechanism leading to the selective synthesis of 2D-like CuO nanoparticle agglomerates via pulsed electrical discharges in water. By adjusting the voltage and pulse width, we selected four experimental conditions: 5 kV/100 ns, 5 kV/500 ns, 20 kV/100 ns and 20 kV/500 ns. In these conditions, we obtained agglomerates with dimensions ranging between 40 and 100 nm, formed of CuO nanoparticles having sizes of few nm. While the morphology of the agglomerates and their composition do not change in all the four conditions, the size, the shape and the crystal properties of the CuO nanoparticles can be finely tuned by choosing the coupling between voltage and pulse width. Moreover, the nanoparticles are more densely agglomerated at longer pulse width. Based on our observations, we infer the growth mechanism of the CuO nanoparticle agglomerates. We demonstrate that CuO nanoparticles are formed during the discharge, while their agglomeration can be mainly due to the pressure gradients existing within the plasma formed by the discharge, which encourage nanoparticle agglomeration on one plane.

Effect of Voltage Pulse Width and Synchronized Substrate Bias in High-Power Impulse Magnetron Sputtering of Zirconium Films

The Zr film microstructure is highly influenced by the energy of the plasma species during the deposition process. The influences of the discharge pulse width, which is the key factor affecting ionization of sputtered species in the high-power impulse magnetron sputtering (HiPIMS) process, on the obtained microstructure of films is investigated in this research. The films deposited at different argon pressure and substrate biasing are compared. With keeping the same average HiPIMS power and duty cycle, the film growth rate of the Zr film decreases with increasing argon pressure and enhancing substrate biasing. In addition, the film growth rate decreases with the elongating HiPIMS pulse width. For the deposition at 1.2 Pa argon, extending the pulse width not only intensifies the ion flux toward the substrate but also increases the fraction of highly charged ions, which alter the microstructure of films from individual hexagonal prism columns into a tightly connected irregular column. Increasing film density leads to higher hardness. Sufficient synchronized negative substrate biasing and longer pulse width, which supports higher mobility of adatoms, causes the preferred orientation of hexagonal α-phase Zr films from (0 0 0 2) to (1 0 1¯ 1). Unlike the deposition at 1.2 Pa, highly charged ions are also found during the short HiPIMS pulse width at 0.8 Pa argon.

Investigation on the generation of high voltage quasi-square pulses with a specific two-node PFN-Marx circuit.

A specific multi-stage two-node pulse forming network (PFN)-Marx topology circuit, which can produce high voltage quasi-square pulses, is investigated and designed in this paper. The specificity of this circuit is that the PFN-Marx is formed by several uniform ladder networks that have two sections of the LC unit and a ladder network formed by four-node capacitors. Compared with conventional PFN-Marx generators formed by equal-value capacitors and inductors with more than four nodes, the number of capacitors in the circuit designed here can be reduced significantly. A five-stage two-node PFN-Marx is assembled to verify the validity of the topology circuit. The experimental results show that the PFN-Marx can generate a quasi-square pulse with a pulse width of 105 ns, flat-top of 50 ns, and amplitude of 80 kV with ±3% voltage variation. The topology circuit design is promising to achieve higher voltage (more stages) and longer pulse width.

High-speed slot-scanning radiography using small-angle tomosynthesis: Investigation of spatial resolution.

This paper studies spatial resolution that is achievable with a fast slot-scanning tomosynthesis approach for orthopedic examinations. Hereby, we use parallel scanning motion implemented in a twin robotic x-ray system. We have measured and analyzed the modulation transfer function (MTF) for various combinations of scanning speed, x-ray tube voltage, pulse length, the nominal focal spot size as well as source-to-object distances. Moreover, we present a theoretical model which describes the system in terms of the MTF. The system was equipped with newly developed linear trajectory prototypes for slot scanning. The acquired images form the basis for a small-angle tomosynthesis reconstruction. In total, three different scanning speeds (27, 14, 8cm/s), pulse lengths (1, 2, 4ms), tube voltages (80, 100, 120kV), two nominal focal spot sizes (0.6, 1.0), and three source-to-object distances (950, 1050, 1150mm) were investigated. To determine the resolution capabilities, we measured the MTF for the given parameter space. The results were then used to design a filter that yields a desired resolution in the reconstructed image. In addition, we also measured the noise power spectrum (NPS) to show the influence of the aforementioned filters on the noise distribution. We have shown that the presented model is in good agreement with the performed measurements. Scanning speed and pulse width have an impact on the MTF in the scanning direction. Up to a travel distance of 0.3mm during an x-ray pulse, an isotropic resolution can be achieved. Longer pulse width or higher scanning speed cause anisotropic resolution. Moreover, it is shown that none of the investigated parameters have an influence on the MTF perpendicular to the scanning direction (slot direction). The 10% MTF value ranges between 9 and 18lp/cm in the scanning direction and about 18lp/cm in slot direction. Tube voltage, nominal focal spot size as well as the source-to-object distance showed no major impact on the system MTF. In terms of the anisotropic resolution capabilities, we have shown that limiting the resolution in the slot direction to obtain isotropic resolution is possible yet at the cost of an inhomogeneous noise pattern. However, maintaining the resolution in slot direction will provide a better edge response and a more homogeneous noise texture at the cost of an inhomogeneous image resolution. We have demonstrated the feasibility of the slot-scanning technique using a twin-robotic x-ray system. Even the fastest scanning mode (27cm/s) yields image resolution on a level that is sufficient for typical orthopedic examinations in terms of musculoskeletal (MSK) measurements. Moreover, it could be shown that the application of specifically designed target MTFs on two-dimensional x-ray images is feasible.

Subjective memory complaints after electroconvulsive therapy: systematic review.

Aims and methodFew studies have looked at subjective memory impairment from electroconvulsive therapy (ECT) after treatment completion. We aimed to systematically review all available evidence for subjective post-treatment effects. RESULTS: We included 16 studies in this review. There was considerable between-study heterogeneity in clinical population, ECT modality and assessment scales used. The most common assessment scale (eight studies) was the Squire Subjective Memory Questionnaire. The majority of studies reported an improvement in subjective memory after ECT, which correlated with improved depression scores. Subjective complaints were fewer in studies that used ultra-brief pulse ECT. Longer pulse widths were associated with more subjective complaints, as was female gender and younger age of treatment in the largest study.Clinical implicationsThere is considerable heterogeneity between studies, limiting meaningful conclusions. Ultra-brief pulse ECT appears to result in fewer subjective complaints.Declaration of interestNone.