Long-term Straw Return Research Articles

The divergent role of straw return in soil O2 dynamics elucidates its confounding effect on soil N2O emission

The divergent effects of straw returns on nitrous oxide (N2O) emissions from soil require elucidation of the underlying mechanisms and factors that explain the inconsistency in in-situ conditions. We conducted a field experiment based on a long-term trial under different regimes of nitrogen (N) fertilization and straw management, complemented by laboratory incubation experiments involving visualized O2 dynamics imaging. In the field trial, we performed hourly basis high-time-resolution measurements of soil matrix oxygen (O2), N2O concentrations and fluxes during N2O “hot moment” events. We found that straw return increased cumulative N2O emissions by 32.7% under conventional high N input (Ncon), but showed no effect on N2O emission under optimized N input (Nopt). In situ O2 content and further microcosm experiments with visualized O2 spatiotemporal distribution suggested that long-term straw return increases porosity and soil O2 content, which reduced N2O emission under low N substrate conditions by improving soil pore structure and aeration during “hot moment” events. By contrast, straw return increased N2O emission via creating short-term O2 depletion zone and triggering denitrification in anoxic microsites when excess N substrate was available. Although straw return showed inconsistent effects on N2O emission under different N application rates, it consistently decreased N2O concentration in the soil matrix during the "hot moment" events, suggesting that straw return increases the transport of the produced N2O in soil matrix to the soil surface. Our study underscores the multifaceted role of straw return in soil O2 dynamics, i.e., stimulating O2 consumption in a short-term microscale of soil, but increasing soil porosity in a long-term mesoscale of soil. This explains the confounding effects of straw management on the production and transportation of soil N2O in situ and emphasizes the importance of optimized N fertilization for reducing the “hot moment” N2O emissions when straw is incorporated.

Long-term straw return increases fungal residual contribution to soil microaggregate nitrogen pool: An eco-enzymatic stoichiometric study

Straw return is a widespread agricultural practice for improving cropland nitrogen (N) stocks. However, the contribution of microbial N to the soil aggregate N pool and the underlying microbial metabolic regulation mechanisms remain uncertain. This study was based on a 13-year field experiment with rice (Oryza sativa L.) and wheat (Triticum aestivum L.) rotation, using only a chemical fertilizer alone (CF) as the control. We analyzed the effects of the chemical fertilizer combined with (CS, 9500 kg ha−1 y−1) and wheat (4000 kg ha−1 y−1) straw on microbial derived-N, microbial carbon (C) and N limitations. We also assessed microbial N use efficiency (NUE) in various aggregates of ferric lixisols (0–20 cm). Rotary tillage reached a depth of 20 cm. The CS significantly increased microbial-derived N concentrations in soil aggregates and enhanced the contribution of fungal residual N to the N pool in aggregates < 0.25 mm, but did not affect those > 0.25 mm. Conversely, the bacterial contribution to the N pool was not affected by CS. Meanwhile, CS significantly increased the soil organic C and microbial biomass in the aggregates. The results of our eco-enzymatic stoichiometric model revealed that the CS significantly alleviated microbial C limitations and increased microbial NUE in soil aggregates. Structural equation modeling further revealed that the microbial biomass and soil organic C contents are key drivers of the microbial C limitation. The increased contribution of fungal residual N to the N pools in < aggregates 0.25 mm was attributed to improved microbial NUE resulting from the straw, without altering net N mineralization rates or β-1,4-N-acetylglucosidase activity. Our findings suggest that straw return promotes microbial-derived N production and sequestration by alleviating microbial C limitation. The strategies governing these microbial-derived N responses in aggregates to straw return might vary. This might be valuable for designing cropland management practices to improve N storage.

Long-term straw return enhanced the chlorine reactivity of soil DOM: Highlighting the molecular-level activity and transformation trade-offs

Chemical properties and molecular diversity of dissolved organic matter (DOM) in agricultural soils are important for soil carbon dynamics and chlorine activity. Yet the chlorine reactivity of soil DOM at the molecular level under agricultural management practices remains unidentified. Here, we investigated the chlorine reactivity of soil DOM under long-term straw return and the molecular activities and transformations during chlorination. The 9-year straw return enhanced the chlorine reactivity of soil DOM, leading to increases in the production of traditional disinfection byproducts (DBPs) and decreases in the formation of emerging high molecular weight DBPs. C17HnOmCl1–2 and C22HnNmOzCl were the highest relative abundances of emerging DBPs. The emerging DBPs were primarily generated through chlorine substitution reactions, with their precursors exhibiting higher H/Cwa (1.47) and O/Cwa (0.41) ratios under straw return. The molecular transformation ability and inactive molecules of soil DOM under long-term straw return were reduced after chlorination, resulting in increased DOM instability. Chlorination led to a shift in the thermodynamic processes of soil DOM molecules from thermodynamically limited to thermodynamically favorable processes, and lignin-like compounds displayed higher potentials for transformation into protein/amino sugar-like compounds. C19H26O6 was identified as a sensitive formula for tracing chlorine reactivity under straw return, and a network illustrating the generation of DBPs from C19H26O6 was established. Overall, these results highlighted the strong chlorine reactivity of soil DOM under long-term straw return.

Variation in Soil Hydrothermal after 29-Year Straw Return in Northeast China during the Freeze–Thaw Process

In seasonal agricultural frozen soil areas, the straw return may influence the freeze–thaw characteristics by changing the soil organic matter and porosity. Monitoring moisture and heat in the freeze–thaw period is significant for preventing spring waterlogging and reasonable planting arrangements. However, the effect of long-term straw return on the soil freeze–thaw process is still unclear. In this study, we investigated the dynamics of soil temperature (ST) and soil moisture (SM) between straw-return cropland (SF) for 29 consecutive years and no-fertilization cropland (NF) during freeze–thaw progress in northeast China. The soil in both sites underwent unidirectional freezing and bidirectional thawing processes. The soil freezing and thawing dates in the NF of the profile occurred earlier than that in the SF. The NF had higher frozen depth and freezing rate than the SF and exhibited a larger range of ST variation and higher heat transmission efficiency. The SM showed a declining trend before the ST started to decrease to a freezing point at different depths in both sites. The migrated SM in most soil layers decreased during monitoring. The relationship between SM and negative ST was a power function at different frozen depths. The SM decreased rapidly in the range of −2–0 °C in both sites. During phase changes, the SF and NF consumed 33.0 and 43.6 MJ m−2, respectively. The results can partially explain the response of straw return to soil hydrothermal variation during the freeze-thaw process. This study may provide an integral theory for effectively utilizing agricultural soil hydrothermal resource in northeast China.

Long-term straw return promotes accumulation of stable soil dissolved organic matter by driving molecular-level activity and diversity

Agricultural management practices play pivotal roles in affecting soil organic matter formation pathways, particularly through changes in soil dissolved organic matter (DOM). The potential effects of long-term straw return on the molecular-level activity and transformations of DOM were investigated in this study. Over 9 years, the soil DOM from straw return had high stability, unsaturation degree, oxidability, and strong aromaticity. The relative abundance of biologically refractory DOM components (i.e., lignin-, condensed aromatic-, and tannin-like formulas) increased by 4 %-33 %, whereas the relative abundance of biodegradable DOM components (i.e., lipids and protein/amino sugar-like formulas) decreased by 15 %-23 % under straw return. Furthermore, the positive effects of straw return on the intergroup transformations of biologically refractory compounds favored the persistence of recalcitrant organic matter in the soil. Importantly, intragroup transformations of recalcitrant-active molecules and biologically refractory compounds were critical pathways for stable DOM accumulation under straw return, and these were mainly driven by thermodynamically limited processes. Thus, our results indicated that long-term straw return promoted the persistence of stabilized soil DOM by driving molecular-level activity and diversity.

Enhancing phosphorus transformation in typical reddish paddy soil from China: Insights on long-term straw return and pig manure application via microbial mechanisms

Effective utilization of organic resources to activate residual phosphorus (P) in soil and enhance its availability is crucial for mitigating P resource scarcity and assessing the sustainable use of P in agricultural practices. However, the mechanisms through which organic resources affect soil P conversion via microorganisms and functional genes remain unknown, particularly in long-term organic-inorganic agricultural systems. In this study, we examined the impact of combined organic-inorganic fertilizer application on P availability, carbon (C) and P cycling genes, and microbial communities (bacterial and fungal) in reddish paddy soil based on a 42-year field experiment. The results indicated that long-term straw returning and pig manure application significantly augmented soil organic carbon (SOC), Olsen-P, microbial biomass carbon (MBC), microbial biomass phosphorus (MBP), enzyme-P, and CaCl2-P levels in paddy soils. Furthermore, these practices increased the abundance of soil C degradation genes, reduced the abundance of soil P cycling genes, and altered microbial community structure and network complexity. Notably, Module #3 ecological clusters in the fungal ecological co-occurrence network were significantly correlated with P cycling genes. Finally, our study demonstrated that long-term straw returning and pig manure application in paddy fields facilitated two robust and contrasting predictive relationships between C degradation (negative relationship) and P cycling (positive relationship) genes, respectively, and enzyme-P and HCl-P changes to improve soil P availability. This study can enhance our understanding of the role of soil microbial communities and functional genes in mediating P transformation to decipher the enhancement in P application efficiency driven by organic resources in reddish paddy soils.

Long-term straw return enhanced crop yield by improving ecosystem multifunctionality and soil quality under triple rotation system: An evidence from a 15 years study

ObjectiveRice-rice-oilseed rape rotation is a typical triple cropping system in Asia, and the development of rice-oilseed rape rotation plays an important role in ensuring global food and oil security. With the intensive cropping rotation and frequent changes between paddy and upland farming under anaerobic and aerobic conditions, whether tillage and straw return would be benefit for both agronomic and environmental perspectives remained to be tested. MethodTherefore, we used a long-term locational experiment (15 years) employing four treatments of conventional tillage without straw incorporation (CT); conventional tillage with straw incorporation (CTS); no-tillage without straw mulching (NT); no-tillage with straw mulching (NTS), to assess the relationships among soil quality, ecosystem multifunctionality, and crop yields under the straw-returning and tillage strategies, based on both agronomic and environmental perspectives. ResultThe results showed that long-term straw return to the field could increase the yield of early rice by 6.24–8.80%, late rice by 3.81–7.13%, and oilseed rape by 9.55–14.45%. Long-term straw return enhanced soil quality index (SQI) by 22.21–40.74% and ecosystem multifunctionality (EMF) by 104.19–141.14% in the 0–20 cm soil layer. Long-term conventional tillage (i.e., CT and CTS) showed higher SQI and EMF values especially in the oilseed rape season. Structural equation modelling (SEM) indicated that straw return and tillage mainly increased crop yield by increasing the SQI in the oilseed rape season. ConclusionIn conclusion, long-term straw return with conventional tillage would be more benefit for the improvement of soil quality and the multifunctionality of soil ecosystems under triple paddy-upland rotation system, offering an effective approach for sustainable soil management and crop production.

Effect of long-term straw return on organic matter transformation by hydroxyl radical during paddy soil oxygenation

The generation of hydroxyl radicals (OH) mediated by Fe(II) has drawn increasing amounts of attention due to its significant role in the transformation of soil organic carbon (SOM) during redox fluctuations in paddy soil. This study further examined the effect of long-term (since 1984) straw return on Fe(II) on driving OH formation and SOM transformation upon paddy soils oxygenation. We found that long-term straw return could inhibit the OH and CO2 production in abiotic processes. The OH production decreased from 190.4 to 133.4 µM as the straw return amount increased from 0 to 19.16 t·hm−2·year−1. Moreover, 14.7–38.6 % of the CO2 production was associated with OH during oxygenation. By combining radical quenching and OM extraction, we determined that straw return could increase O2 reduction to H2O2 through a two-electron transfer pathway. Based on chemical extraction, Mössbauer spectra and multilinear regression model, we demonstrated that straw return resulted in more active Fe(II) species that controls the OH production. The results of 3DEEMs and FT-ICR MS showed that oxidative transformation can be accomplished by the introduction of OH to aromatic structures or olefins through ring opening and hydroxylation mechanisms. And OM at high straw return concentrations was likely oxidized by OH into bioavailable aliphatic compounds. Therefore, this study highlights the underappreciating effects of long-term straw return on Fe(II) oxidation in driving OH generation and SOM transformation in paddy soil redox fluctuation events.

Effects of Straw Return Duration on Soil Carbon Fractions and Wheat Yield in Rice–Wheat Cropping System

In China’s subtropical rice–wheat cropping system, the changes in the soil organic carbon (SOC) pool due to long-term straw return and its connection with crop yield remain unclear. This study aims to provide insights into establishing a sensible straw return system by evaluating the differences in the distribution and variation rates of SOC, light fraction organic carbon (LFOC), heavy fraction organic carbon (HFOC), particulate organic carbon (POC), and mineral-associated organic carbon (MOC) in the 0–20 cm soil layer under different durations of straw return. Additionally, the study analyzes the relationship between the changes in SOC and its fractions and wheat yield. The experiment was conducted in 2019 in a rice–wheat rotation field with ten years of straw return treatments: no straw return (NR) or 1, 2, 3, 4, 5, 6, 7, 8, 9 year(s) of straw return (SR1–9), and an additional treatment in 2020 (10 years of straw return, SR10). The results revealed that with an increase in the duration of straw return, the contents of SOC, LFOC, HFOC, and POC gradually increased, showing the highest increments of 45.88%, 187.22%, 41.55%, 97.89%, and 28.21%, respectively, compared to the NR treatment. However, after eight years of straw return, the compound annual increase in soil organic carbon and its components was lower than in years 1–8, indicating a trend of diminishing increments. The SOC content and its variation were significantly correlated with the content and variation of LFOC, HFOC, POC, and MOC, with the highest sensitivity observed for the variation in LFOC, indicating the strong influence of the duration of straw return. The SOC and its fraction contents showed significant positive correlations with wheat yield, with the highest contribution to wheat yield increase attributed to an increase in LFOC content. In summary, straw return enhances the 0–20 cm deep soil carbon pool, with LFOC being the most sensitive indicator, reflecting the influence of the duration of straw return on soil carbon pools.

Long-term straw returning improved soil nitrogen sequestration by accelerating the accumulation of amino acid nitrogen

Straw incorporation has been widely recommended as an effective management practice to increase soil nitrogen stocks in agroecosystems worldwide. However, the overall trend and magnitude of changes in various soil organic nitrogen fractions in response to crop residue return and their contribution to soil nitrogen sequestration remain uncertain. Here, we show through a long-term experiment (12 years) that straw return increased soil total nitrogen and soil nitrogen sequestration rate by 20.01% and 120.95%, respectively. Ammonia nitrogen, amino acid nitrogen, and amino sugar nitrogen were affected by straw and its interaction with year, and increased by 43.08%, 27.70%, and 46.56%, respectively, with the highest growth rate in amino acid nitrogen (10.51 mg kg−1 yr−1). Furthermore, redundancy analyses showed that soil total organic carbon and C:N ratio were the major factors explaining the variations in soil organic nitrogen fractions, which increased by 46.22% and 21.75%, respectively. The structural equation model further revealed that amino acid nitrogen was a pivotal driver of soil nitrogen sequestration, because the soil C:N ratio indirectly influenced soil nitrogen sequestration via affecting the accumulation of amino acid nitrogen. Our results suggest that long-term straw return can improve nitrogen sequestration by accelerating the accumulation of amino acid nitrogen and that soil nitrogen storage and supply capacity may be improved by adopting straw incorporation and other appropriate management practices to regulate soil stoichiometry.

Transcriptome Analysis of Maize Ear Leaves Treated with Long-Term Straw Return plus Nitrogen Fertilizer under the Wheat-Maize Rotation System.

Straw return (SR) plus nitrogen (N) fertilizer has become a practical field management mode to improve soil fertility and crop yield in North China. This study aims to explore the relationship among organic waste, mineral nutrient utilization, and crop yield under SRN mode. The fertilizer treatments included unfertilized (CK), SR (straws from wheat and corn), N fertilizer (N), and SR plus N fertilizer (SRN). SRN treatment not only significantly increased the grain yield, net photosynthetic rate, and transpiration rate but also enhanced the contents of chlorophyll, soluble sugar, and soluble protein and increased the activities of antioxidant enzymes but reduced intercellular CO2 concentration and malondialdehyde (MDA) content when compared to other treatments. There were 2572, 1258, and 3395 differentially expressed genes (DEGs) identified from the paired comparisons of SRvsCK, NvsCK, and SRNvsCK, respectively. The transcript levels of many promising genes involved in the transport and assimilation of potassium, phosphate, and nitrogen, as well as the metabolisms of sugar, lipid, and protein, were down-regulated by straw returning under N treatment. SRN treatment maintained the maximum maize grain yield by regulating a series of genes' expressions to reduce nutrient shortage stress and to enhance the photosynthesis of ear leaves at the maize grain filling stage. This study would deepen the understanding of complex molecular mechanisms among organic waste, mineral nutrient utilization, crop yield, and quality.

Long-term straw return improves cooked indica rice texture by altering starch structural, physicochemical properties in South China

Straw return can improve rice eating quality by modifying starch formation from long-term field trials, whereas the relevant mechanisms are still unknown. A long-term field experiment, including straw removal (CK), straw burning return (SBR), and straw return (SR) was conducted to investigate the starch structure, physicochemical properties, and cooked rice textures of indica early- and late-rice. Compared with CK, SBR and SR enhanced relative crystallinity, amylopectin long chains in both rice seasons, and gelatinization temperatures in late rice. Compared to SBR, SR decreased protein content and amylopectin short chains but increased starch branching degree, breakdown, and stickiness, ultimately contributing to improved starch thermal and pasting properties. Meanwhile, SR decreased hardness, cohesiveness, and chewiness, resulting in cooked texture meliorated, which was mainly attributed to amylopectin chain length and starch granule size. The results suggest that SR increased cooked texture of indica rice by altering starch structural and physicochemical properties.

Uncovering the potentials of long-term straw return and nitrogen supply on subtropical maize (Zea mays L.) photosynthesis and grain yield

ContextCrop straw is a significant byproduct of agricultural production and a rich source of nutrients, but it is carelessly disposed of, potentially harming the environment and wasting valuable resources. In China, returning straw to the soil as a means of disposing of agricultural waste is a crucial management technique that not only makes use of agricultural waste but also enhances soil quality and grain yield. ObjectiveA five-year (2018–2022) field experiment was designed to study the effect of straw treatment methods and optimal nitrogen (N) application rate on the photosynthesis process and grain yield of maize. MethodsThe different straw methods (straw return (SR) and traditional practice (TP) — no straw return) were assigned to main plots while the N levels such as N100 (100 kg ha−1), N150 (150 kg ha−1), N200 (200 kg ha−1), N250 (250 kg ha−1), and N300 (300 kg ha−1) were allotted to sub-plots. ResultsThe results showed the photosynthesis rate (Pn), intercellular carbon dioxide concentration, stomatal conductance, transpiration rate, relative chlorophyll content (SPAD), leaf area index (LAI), dry matter (DM), soil nitrogen content, maize grain nitrogen accumulation, and grain yield were increased with an increase in N levels. Similarly, SR also showed a promotion effect on the aforementioned parameters compared with TP. After five years, SR with an optimum N level (250 kg ha−1) increased the SPAD values (2%) and Pn (8%) which resulted in higher LAI (27%) compared with TPN300. As a result, more DM (15%) was formed and finally increased the grain yield (7%). It was revealed that the SPAD, Pn, LAI, DM, and grain yield were maximum at a higher 300 kg N ha−1 level but it was found statistically similar with 250 kg N ha−1. So in this study, SR with 250 kg N ha−1 is optimum for better photosynthetic efficiency, dry matter production, and grain yield. The correlation analysis also showed a positive correlation (i) of SPAD values with Pn (ii) LAI with SPAD values and Pn, (iii) DM with SPAD values, Pn, and LAI, and (iv) grain yield with DM under SR compared with TP. Conclusion and implicationIn summary, SRN250 resulted in better chlorophyll content and photosynthesis process that yielded more photosynthetic area for higher DM production and subsequently higher maize grain yield. The SR reduced the amount of N fertilizer and improved the grain yield and it is the best approach to improve grain yield and minimize agricultural waste production. Long-term trials are needed to understand the effects of straw incorporation and nitrogen application in different regions and locations.

Temporal dynamics of density separated soil organic carbon pools as revealed by δ13C changes under 17 years of straw return

Straw return is widely recommended as an effective measure to increase the amount of soil organic carbon (SOC) and improve soil fertility in agroecosystems. However, the temporal dynamics of the original and newly derived SOC during long-term straw return remain uncertain. This study aimed to (i) determine the temporal dynamics of original and newly derived SOC in various soil organic matter (SOM) fractions during cultivation and (ii) evaluate the effect of continuous straw return on the contents of original and newly derived SOC. These aims were achieved by analyzing topsoil samples (0–20 cm) in a 17-year field experiment using a continuous maize (Zea mays L.) cropping system. Three fertilizer treatments were tested: no fertilizer (NF), mineral fertilizer (NPK), and mineral fertilizer with straw return (NPKS). The SOC was physically separated into three density fractions: the free light fraction (fLFOC), occluded light fraction (oLFOC), and heavy fraction (HFOC), and their δ13C values were determined. The original and newly derived SOC of each fraction and the rate of conversion of exogenous C inputs were evaluated. The SOC content in the bulk soil and C fractions generally increased continually with cultivation time in the NPKS, indicating that the soil had not reached maximum C sequestration. NPKS significantly increased the C contents of fLFOC, oLFOC, and HFOC by 38.6%, 43.0%, and 11.6%, respectively, relative to the initial soil after the 17-year experiment. The newly derived SOC content was significantly higher in NPKS than in NF and NPK, and the rate of conversion of exogenous C inputs to SOC was the lowest in NPKS. The annual rate of loss of original SOC in NPKS was lowest for the light fractions (fLFOC and oLFOC) and highest for HFOC, implying that the return of straw moderated the mineralization of original SOC in the light fractions but increased the decomposition of original SOC in the heavy fraction via the priming effect (PE). The average rate of conversion of exogenous C inputs remained the highest in HFOC (23.5%), lowest in oLFOC (0.6%), and intermediate in fLFOC (4.1%) throughout the study. Thus, the stable HFOC pool retained more straw C, despite the stronger PE. Our results demonstrated that long-term straw return continuously increased the proportion of new SOC in the bulk soil and density fractions and increased the decomposition of the original SOC in the stable SOC fractions. These findings provide further detailed insights into the mechanisms of SOC stabilization in agricultural soils.

Quantification of the effects of long-term straw return on soil organic matter spatiotemporal variation: a case study in a typical black soil region

Abstract. The straw return practice is essential to soil organic matter (SOM) accumulation in the black soil area with high carbon sequestration potential. However, due to lacking accurate spatial distribution of straw return, few studies have carried out rigorous research on the impact of long-term straw return on SOM spatiotemporal variation on a regional scale. This study was carried out across an approximately 3000 km2 area in Lishu County, northeastern China, a typical agricultural plain. Based on a total of 619 soil samples and 16 environmental covariates, the study mapped the spatial distributions of SOM in 2006 and 2018 by random forest (RF) and evaluated the effects of the interaction of soil properties, land use, and straw return on SOM spatial–temporal variation. The results show that in the context of long-term straw return, the mean SOM content increased from 18.93 to 20.84 g kg−1 during 2006–2018. And 74.49 % of the region had a significant increase (maximum of 24.41 g kg−1) of SOM. The severest SOM loss occurred in the northwest due to the light texture and the transition from paddy fields to dryland. Nevertheless, for areas from paddy fields to dryland, the SOM loss decreased with the increased cumulative crop residue coverage (CRC). The SOM even increased by 1.79 g kg−1 when the cumulative crop residue coverage reached 0.60–1.00. In addition, soil with higher initial SOM and sand content had a lower response to straw return. The study revealed that straw return is beneficial to carbon sink in farmland and is a better way to prevent a carbon source caused by the conservation of paddy field to dryland.

Effects of Combined Long-Term Straw Return and Nitrogen Fertilization on Wheat Productivity and Soil Properties in the Wheat-Maize-Soybean Rotation System in the Pannonian Plain

The study, conducted to evaluate the effects of long-term straw management combined with the application of increasing nitrogen rates on the yield of twenty winter wheat varieties, as well as on soil properties, was carried out in a long-term field trial established in 1971. The trial was monitored for twenty growing seasons under rainfed conditions in a typical chernozem zone of the southern part of the Pannonian Plain. The cropping system was a winter wheat-maize-soybean rotation. The ten SN-treatments (combinations of straw management (S) and N-fertilization) were as follows: In the plot (treatment) with straw return (S1), seven variants of nitrogen fertilization (0–180 kg N ha−1) were included, while on the plot without straw return (S0) the variants of N-fertilization were 0, 90 and 150 kg N ha−l. Based on the high relative share in the total sum of squares, variance analysis showed that wheat grain yield (GY) was significantly affected by years, SN-treatments, and their interaction, and they can explain the largest part of the total variance of GY. The results showed that straw return integrated with N fertilization could increase wheat yield to varying degrees over 20 years. On average, for all years, the highest GYs were obtained in the treatment S1 and fertilization with 180 and 150 kg N ha−1. The overall results showed that long-term straw returning significantly increased GY by an average of 8.4 ± 4.5%, with a considerable simultaneous increase in yield stability compared to straw removal. In addition, straw incorporation (SI) significantly increased soil humus, total nitrogen (TN), and soil organic carbon (SOC) contents at a soil depth of 0–30 cm by an average of 4.2, 3.8, and 11.3%, respectively. The results of our study have demonstrated that the long-term practice of straw return, in combination with the application of mineral fertilizers, has the potential to serve as a sustainable soil management strategy that is economically viable and environmentally acceptable. However, additional research is required to investigate its interactive effects on both grain yield and soil productivity.

Effects of 24 Years Different Straw Return on Soil Carbon, Nitrogen, Phosphorus, and Extracellular Enzymatic Stoichiometry in Dryland of the Loess Plateau, China

Exploring the biogeochemical cycle characteristics of soil carbon, nitrogen, and phosphorus in farmland in the dryland of the loess plateau can provide scientific basis and technical support for efficient crop production and sustainable land use. Here, based on a long-term (24 year) straw return field experiment in Shouyang, Shanxi province, the effects of different straw return regimes, i.e., straw mulching (SM), direct straw return (DS), animal-digested straw return (AS), and non-straw return (CK), on the stoichiometric ratio of soil elements and extracellular enzyme activities were studied. The vector angle and length were calculated to indicate the resource constraints faced by microorganisms. The vector angle was greater than 45° and less than 45°, indicating that microorganisms were limited by phosphorus and nitrogen, respectively. The greater the deviation from 45°, the greater the degree of limitation, and the longer the vector length, the more severely limited by carbon. The results showed that ① the soil C/N and C/P of long-term straw returning ranged from 9.81 to 14.28 and from 14.58 to 21.92, with the mean values of 12.36 and 17.51, respectively, which were 6.0% and 4.2% lower than that at the initial stage of the experiment. The soil N/P was distributed between 1.27 and 1.57, with an average of 1.42, which was 2.2% higher than that in the initial stage. The soil C/N and C/P ratios showed a trend of first decreasing and then increasing, the soil N/P ratio basically showed a flat trend, and there was no significant difference in soil element metering ratios between different straw returning treatments. ② Compared with the 24-year long-term non-straw return treatment, the activities of β-1,4-glucosidase (BG) and β-1,4-N-acetylglucosaminidase (NAG) in the soil of the long-term straw mulching treatment increased by 134.4% and 107.5% (P<0.05), the activities of BG and alkaline phosphatase (AP) in the soil of the long-term straw mulching treatment decreased by 59.3% and 59.5% (P<0.05), respectively, and the activities of NAG in the soil of the long-term straw mulching treatment increased by 102.8% (P<0.05). Under the long-term straw returning treatment, soil microorganisms were faced with carbon and phosphorus limitation as a whole. Long-term straw mulching aggravated microbial carbon limitation, and animal-digested straw return could alleviate the degree of carbon limitation. Compared with that in the 24-year long-term non-straw return treatment, soil EEAC/N could be significantly reduced by the animal-digested straw return treatment, and soil EEAC/P could be increased by the direct straw return treatment. The three straw returning methods had no significant indigenous effect on soil EEAN/P. The overall vector angle was greater than 45°, and the vector length increased by 3.8%-20.1% compared with that in the initial stage. ③ Correlation analysis showed that C and N inputs were significantly negatively correlated with BG activity; available nitrogen was significantly correlated with NAG activity, AP activity, and EEAC/N; C/P was significantly positively correlated with EEAC/N; there were significant correlations between N/P and NAG activity, AP activity, EEAC/N, and EEAC/P; and there was no significant correlation between EEAN/P and any environmental factors. In conclusion, the availability of soil nitrogen and phosphorus elements and N/P ratio had significant effects on soil extracellular enzyme activity and stoichiometric characteristics under different long-term straw returning treatments. In the future, more attention should be paid to the improvement of organic carbon and the promotion of nitrogen and phosphorus availability in farmland soil in soil-efficient cultivation and agricultural production activities.

Crop responses to application of optimum nitrogen fertilizers on soils of various fertilities formed from long-term fertilization regimes

How crops uptake synthetic nitrogen (N) applied to soils of differing fertilities formed from long-term fertilization regimes is a noteworthy scientific question that remains unclear. This is important for understanding nitrogen use efficiency (NUE) and N losses of synthetic N input to soils of different quality. Here, we investigated crop biomass, N uptake, root growth, and NUE in response to three synthetic N rates (including no N fertilizer, No-N; optimum N, Opt.-N; original N, Ori.-N) in long-term plots (since 2006), with various background fertilities induced by long-term fertilization regimes (nil N, optimum synthetic N, conventional synthetic N, and balanced synthetic N and manure) in a cropping system of winter wheat–summer maize rotation. When Opt.-N was applied, no significant differences in crop biomass between soils. However, N uptake by the crops on nil N soils was lower than that on N-fertilized soils. Crop biomass and N uptake in the nil N soils were significantly lower than those in the N-fertilized soils under No-N and Ori.-N supply, and no significant differences were observed between the N-fertilized soils. When Opt.-N was supplied to the nil N soils, grain yield and N uptake increased by 126–191% and 138–148%, respectively, compared with No-N. This change was associated with a large increase in root mass and root length density in the soils. In optimum synthetic N soils, the crop maintained a high yield (7.0–8.2 Mg ha−1) and N uptake (136–228 kg N ha−1) when they received continuous Opt.-N rate. Crop yield and N uptake significantly decreased when N fertilizer supply was ceased, indicating a low legacy effect; however, the long-term straw return alleviated the above decline. Conventional synthetic N soils with long-term excessive N application continued to maintain a comparable high yield (6.1–7.0 Mg ha−1) and N uptake (128–206 kg N ha−1) even after N fertilizer supply was stopped, indicating a high legacy effect. This effect could be applied with less N fertilizer to reduce N losses. Balanced synthetic N and manure could provide resistance to N fertilizer reduction and maintain high yield and soil fertility even after N fertilizer supply is ceased. Our results highlight the strong resilience of soils to crop productivity when resupplying N fertilizer to long-term nil N soils and the different legacy effects from historic fertilization regimes, while emphasizing that balanced synthetic N and manure is an ideal measure for sustainable N management.

Profile soil organic and inorganic carbon sequestration in maize cropland after long-term straw return

Promoting cropland carbon (C) sequestration through straw return has always been the focus of numerous studies. However, there is still a lack of comprehensive understanding of the straw return effects on soil organic carbon (SOC) and soil inorganic carbon (SIC) sequestration. Therefore, the present study aims to investigate the effects of long-term straw return on SOC and SIC sequestration across the 0–100 cm soil profile in the maize planting cropland in Northeastern China. The results showed an increasing trend in SOC contents in the 0–100 cm soil profile following long-term straw return, while significant decreases in SIC contents were observed in the surface (0–20 cm) and subsoil (20–60 cm) layers, respectively. In addition, the SOC stock increased significantly in the subsoil layer following long-term straw return, by an average value of 44%, which is higher than those observed in other soil layers. On the other hand, the SIC stock in the subsoil layer increased by an average value of 24% and decreased in the surface and under-subsoil layers by average values of 53% and 33%, respectively. Moreover, the exchangeable calcium contents were positively correlated with SOC and SIC stock, demonstrating the soil calcium contributes to SOC and SIC sequestration. The present study highlighted the importance of the subsoil layer for effective straw return strategies in cropland to promote SOC and SIC sequestration in croplands.

Microbial nutrient limitations limit carbon sequestration but promote nitrogen and phosphorus cycling: A case study in an agroecosystem with long-term straw return

Soil fertility can be increased by returning crop residues to fields due to the cooperative regulation of microbial metabolism of carbon (C) and nutrients. However, the dose-effect of straw on the soil C and nutrient retention and its underlying coupled microbial metabolic processes of C and nutrients remain poorly understood. Here, we conducted a comprehensive study on soil nutrients and stoichiometry, crop nutrient uptake and production, microbial metabolic characteristics and functional attributes using a long-term straw input field experiment. We estimated the microbial metabolic limitations and efficiency of C and nitrogen (N) use (CUE and NUE) via an enzyme-based vector-TER model, biogeochemical-equilibrium model and mass balance equation, respectively. In addition, the absolute abundances of 20 functional genes involved in the N- and P-cycles were quantified by quantitative PCR-based chip technology. As expected, straw input significantly increased C and N stocks, C: nutrients, crop nutrient uptake and growth. However, the C sequestration efficiency decreased by approximately 6.1 %, and the N2O emission rate increased by 0.5–1.0 times with the increase in straw input rate. Interestingly, the microbial metabolism was more limited by P when straw input was <8 t ha−1 but was reversed when straw input was 12 t ha−1. The enhanced nutrient limitation reduced both the CUE and the NUE of microbes and then upregulated genes associated with the hydrolysis of C, the mineralization of N and P, and denitrification, which consequently influenced C and N losses as well as crop growth. This study highlights that soil C and nutrient cycling are strongly regulated by microbial metabolic limitation, suggesting that adding the appropriate limiting nutrients to reduce nutrient imbalances caused by straw input is conducive to maximizing the ecological benefits of straw return.