ABSTRACT The individual response to load is multifactorial and complicated by transient temporal changes in biological maturation. The period surrounding peak height velocity exposes potentially “fragile” individuals to systematic, age-related increases in training loads. Bio-banding allows practitioners to manage the biological diversity and align training to the individual development needs . This study explores the acute impact of maturation on neuromuscular performance and perceived intensity through comparing both chronological and bio-banded training sessions. 55 male soccer players (mean ± SD; age 13.8 ± 1.4 years) were recruited from an EPPP academy. Following a warm-up and standardised sub-maximal run (30–15IFT), players competed in five bouts of 5-min 6v6 small-sided games (SSGs) before repeating the standardised sub-maximal run. The sessions were repeated on three occasions with chronological SSGs and the same with bio-banded SSGs wearing foot-mounted inertial measurement units (PlayerMakerTM) with differential ratings of perceived exertion used to quantify internal loads. Mixed linear modelling indicated maturity-specific pre–post differences in neuromuscular response, stride length and cadence having contrasting responses pre- (reduced) and post-PHV (increased), and larger changes in post sessions stiffness for pre- (∼18.6 kN·m−1) and circa-PHV (∼12.1 kN·m− 1) players. Secondly, there were small to large differences in neuromuscular response (RSI, stride length, stiffness, and contact time) and perceptions of intensity between conditions, with bio-banding generally reducing pre–post changes. Bio-banding may therefore offer a mechanism to prescribe maturity-specific training loads which may help to alleviate the impact of repeated exposure to high-intensity activity, thus reducing injury risk whilst promoting long-term player development. Highlights Utilising a sub-maximal running protocol (30–15IFT) with foot mounted accelerometers can detect maturity specific responses to football specific training activity, which aligns with subjective perceptions of intensity. Chronologically derived small-sided games elicit different acute responses between players of varying maturity status, which is somewhat negated when bio-banded small-sided games are used instead. Bio-banding training sessions may offer practitioners a practical way of managing maturity-specific trainings load to reduce injury risk and promote long-term players development.