Based on glacio-meteorological records, 7 years of in-situ mass balance data, and a temperature-index model, the long-term annual and seasonal mass balances of Shiyi Glacier in the northeast Tibetan Plateau (TP) were reconstructed from 1963/64 to 2016/17. Variations were then linked to local climatic and macroscale circulation changes. The model was calibrated based on in-situ mass balance data and was driven by daily air temperature and precipitation data recorded at nearby alpine meteorological stations. The results show that the reconstructed annual mass balance experienced an overall downward trend over the past 54 years, with a remarkably high mass loss rate during 1990/91–2016/17. Analysis of mass balance sensitivity and local climatic changes shows that the pronounced mass loss since the 1990s can be mainly attributed to cumulative positive temperature increases caused by air temperature increases and prolongation of the ablation season. From the perspective of macroscale circulation, the reconstructed annual mass balance values correlate well with zonal wind speeds (June to September) in the glacierized region. For the positive/negative phase of the annual mass balance, an inverse spatial pattern in relation to geopotential height change (low/high-pressure centres) and corresponding conversion of cyclonic/anti-cyclonic circulation were present in northern hemisphere mid-latitudes. Comparative analysis of existing long-term mass balance series over the TP indicates that asynchronous climatic changes in the different glacierized regions led to inconsistent interannual fluctuations in glacier mass balance.