Long-term Biosolids Application Research Articles

Impact of long-term application of biosolids to surface water nitrogen in a working forest plantation in King County, Washington

Forest fertilization with municipal biosolids has been shown to increase tree growth and enhance forest soils. However, there are concerns that nitrogen from the biosolids could impact surface waters through movement from subsurface flow. Here we analyzed data on soil and surface water nitrogen from a working tree plantation that has used biosolids for over three decades to see if there was evidence of N movement through the soil to surface waters. GIS (Geographic Information System) was used to map application units over time and LiDAR (Light Detection and Ranging) was used to delineate watersheds. The program is located in King County Washington with biosolids provided by the King County Wastewater Treatment program. We assembled records to determine if there is any evidence of movement of NO3− through soils or any enrichment in surface waters. While soils show evidence of NO3− enrichment following biosolids application with cumulative loading rates up to 26 Mg ha−1, this is generally limited to the ’A’ soil horizon and does not increase linearly with increased biosolids loading rates. There was no indication of increased surface water NO3− concentration relative to biosolids application rates, with a small trend of decreasing water NO3− over time. Surface water NO3− concentration was not correlated with the fraction of the watershed area that had been amended with biosolids, and there was no observable increase in surface water NO3− with increased frequency of biosolids applications to the tree plantations. These results suggest that the current biosolids program is sufficiently protective of ground and surface waters. These observations suggest that biosolids application can be conducted on a large scale with multiple benefits and no discernible impact to surface waters.

Analysis of effects and factors linked to soil microbial populations and nitrogen cycling under long-term biosolids application

Information about impacts of long-term biosolids application on soil microbial populations and functional groups and N cycling is important for evaluating soil health and agroecosystem sustainability under long-term biosolids application. Mine spoil plots received annual biosolids application from 1973 to 2010 at low (16.8 Mg ha−1 yr−1), medium (33.6 Mg ha−1 yr−1), and high rates (67.2 Mg ha−1 yr−1). A no-biosolids control received chemical fertilizer at the agronomic rate. Soil samples were collected in three seasons per year spanning 2003–2005 for measuring soil moisture, pH, soil organic C (SOC), total and extractable heavy metals (Cd, Cu, Ni, Zn), NO3−, N mineralization potential (NMP), microbial biomass C (MBC), and populations of three N-cycling bacteria (NCB) groups: ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB), and denitrifying bacteria (DNB). Soil samples were collected again in 2008 and 2010 for quantifying total and extractable heavy metals, and in 2018 (eight years after biosolids applications ended) for measuring SOC, MBC, NMP, and microbial respiration. During 2003–2005, mean MBC was 315, 554, 794, and 1001 mg kg−1 in the control, low, medium, and high biosolids treatments, respectively. Populations of NCB did not differ among treatments. Biosolids application increased total and extractable metal concentrations but the effect of biosolids rates were much lower on extractable than total concentrations. Soil extractable Cd and Cu concentrations decreased from medium to high applications, likely due to complexing with biosolids organic matter. Partial least squares regression analysis identified a strong positive effect on MBC of SOC and a weak negative effect of Cu, explaining the strong net positive effect of biosolids on MBC. In 2018, the medium and high biosolids treatments maintained higher SOC, MBC, NMP, and microbial respiration than the control. This study provided further evidence that long-term biosolids application has positive effects on soil microbes that persist for years after ending application.

Using Prescribed Fire and Biosolids Applications as Grassland Management Tools: Do Wildlife Respond?

Prescribed burning is a management tool commonly used in forested ecosystems in the southeastern United States, but the influence of this method on grassland vegetation and wildlife in this geographic region is unknown. During 2009–2015, we conducted a study to determine if the application of prescribed burning and/or long-term biosolid applications alter plant communities and/or wildlife use of grassland areas at Marine Corps Air Station Cherry Point, Havelock, NC. We monitored vegetation growth, measured plant community composition, and documented wildlife activity in four study plots for 3 years after the implementation of annual winter prescribed burns. Prescribed burning reduced the amount of litter, increased bare ground during spring, and altered the plant community composition relative to areas that were not burned. Overall, prescribed burning did not alter (F1,803 = 0.37, p = 0.54) bird use of the airfield grasslands, while the long-term application of biosolids resulted in higher (F1,803 = 17.61, p < 0.01) bird use. Few species-specific differences in avian use of prescribed burned and unburned grasslands were found. In contrast, white-tailed deer (Odocoileus virginianus) use of areas that were burned in winter, as well as the adjacent unburned areas, was drastically reduced. Winter prescribed burning appeared to remove forage plants at the time of year deer would use them the most. Our findings suggest that prescribed burning and biosolid applications, used alone and in combination, might be viable grassland management tools for altering wildlife use of grassland areas, specifically white-tailed deer; however, similar research at additional locations should be conducted.

Heavy metals drive microbial community assembly process in farmland with long-term biosolids application

Biosolids are considered an alternative to chemical fertilizers due to their rich nutrients. However, long-term biosolids application can lead to heavy metals accumulation, which severely affects soil microbial community compositions. The factors influencing soil microbial community assembly were explored under a 16-year long-term experiment with biosolids applications. Our results indicated that biosolids application significantly increased fungal richness while not for bacterial and arbuscular mycorrhizal (AM) fungal richness. Besides, biosolids application significantly affected soil bacterial, fungal compositions and AM fungal community. Soil microorganisms were clustered into different modules with bacterial and AM fungal communities were affected by both organic matter and heavy metals, while fungal communities were affected by heavy metals (Cr, Ni, and As). The soil bacterial community assembly was dominated by stochastic processes while the fungal and AM fungal community assemblies were mainly driven by deterministic processes. Random forest analysis showed that heavy metals were identified as major drivers (Hg, Cu, Cd, and Zn for bacteria, Pb and Cr for fungi, and As and Ni for AM fungi) of the community assembly process. Overall, our study highlights the significant role of heavy metals in shaping microbial community dynamics and gives a guide for controlling biosolids application.

Accumulation of microplastics in soil after long-term application of biosolids and atmospheric deposition

Land-applied biosolids can be a considerable source of microplastics in soils. Previous studies reported microplastics accumulation in soils from biosolid application, however, little is known about the contribution of atmospherically deposited microplastics to agricultural soils. In this study, we quantified and characterized microplastics in soils that have been amended with biosolids over the past 23 years. We also collected atmospheric deposition samples to determine the amount and type of plastics added to soils through atmospheric input over a period of about 2 years. Soil samples were taken from a replicated field trial where biosolids have been applied at rates of 0, 4.8, 6.9, and 9.0 t/ha every second crop. The biosolids were anaerobically digested and dewatered, and were applied by spreading onto the soil surface. Soil and atmospheric samples were extracted for microplastics by Fenton's reaction to remove organic matter followed by flotation in a zinc chloride solution to separate plastic from soil particles. Samples were analyzed for microplastics by optical microscopy and Laser Direct Infrared Imaging Analysis (LDIR). The mean number of microplastics identified from biosolids samples was 12,000 particles/kg dry biosolids. The long-term applications of biosolids to the soil led to mean plastics concentrations of 383, 500, and 361 particles/kg dry soil in the 0–10 cm depth for low, medium, and high biosolids application rates, respectively. These plastic concentrations were not significantly different from each other, but significantly higher than those found in non biosolids-amended soil (117 particles/kg dry soil). The dominant plastic types by number found in biosolids were polyurethane, followed by polyethylene, and polyamide. The most abundant plastics in soil samples were polyurethane, polyethylene terephthalate, polyamide, and polyethylene. Atmospheric deposition contributed to 15 particles/kg dry soil per year and was mainly composed of polyamide fibers. This study shows that long-term application of biosolids led to an accumulation of microplastics in soil, but that atmospheric deposition also contributes a considerable input of microplastics.

Restriction of biosolids returning to land: Fate of antibiotic resistance genes in soils after long-term biosolids application

Although the utilization of biosolids in agricultural lands is widely considered as an effective way to improve resource reuse, the presence of antibiotic resistance genes (ARGs) severely restricts biosolids returning to fields. A 12-year long-term experiment with different biosolids application rates (from 0 to 36 t ha−1 yr−1) was conducted to study the effect of biosolids application on shaping ARGs in soil. Biosolids application significantly increased ARGs abundance in the soil, except for MBS treatment (9 t ha−1 yr−1 biosolids application). The abundance of ARGs in soil did not increase linearly with the dose of biosolids applied, but they were significantly (P < 0.05) positively correlated. A total of 173 subtypes were detected, among them mobile genetic elements (MGEs), aminoglycoside, and multidrug resistance genes were the most dominant types. Except for MBS treatment, most of the ARGs detected were enriched in amended soils after long-term continuous biosolids application. Specifically, tetPA, sul1, mefA, and IS6100 were highly enriched in all amended soils. In addition, biosolids application increased soil nutrients and heavy metals, and changed the soil microbial community, all of which affected ARGs formation. But MGEs may be a greater factor for shaping ARGs profiles than soil properties. Overall, controlling the rate of biosolid application is the key to reducing the accumulation and horizontal transfer of ARGs in soils.

Long-Term Biosolids Application on Land: Beneficial Recycling of Nutrients or Eutrophication of Agroecosystems?

The impact of repeated application of alkaline biosolids (sewage sludge) products over more than a decade on soil concentrations of nutrients and trace metals, and potential for uptake of these elements by crops was investigated by analyzing soils from farm fields near Oklahoma City. Total, extractable (by the Modified Morgan test), and water-soluble elements, including macronutrients and trace metals, were measured in biosolids-amended soils and, for comparison, in soils that had received little or no biosolids. Soil testing showed that the biosolids-amended soils had higher pH and contained greater concentrations of organic carbon, N, S, P, and Ca than the control soils. Soil extractable P concentrations in the biosolids-amended soils averaged at least 10 times the recommended upper limit for agricultural soils, with P in the amended soils more labile and soluble than the P in control soils. Several trace elements (most notably Zn, Cu, and Mo) had higher total and extractable concentrations in the amended soils compared to the controls. A radish plant assay revealed greater phytoavailability of Zn, P, Mo, and S (but not Cu) in the amended soils. The excess extractable and soluble P in these biosolids-amended soils has created a long-term source of slow-release P that may contribute to the eutrophication of adjacent surface waters and contamination of groundwater. While the beneficial effects of increased soil organic carbon on measures of “soil health” have been emphasized in past studies of long-term biosolids application, the present study reveals that these benefits may be offset by negative impacts on soils, crops, and the environment from excessive nutrient loading.

Soil carbon response to long-term biosolids application.

Astudy was conducted in three agroecosystems in California (Sacramento, Solano, and Merced counties) that received biosolids applications for 20 yr. Management varied in application rates and frequencies, resulting in average cumulative amount of biosolids applied of 74 (Solano), 105 (Merced), and 359 (Sacramento) Mg biosolidsdry ha-1 , resulting in the addition of 26 (Solano), 36 (Merced), and 125 (Sacramento) Mg biosolids-C ha-1 . Measurements included soil organic carbon (SOC) and total nitrogen (N) concentrations from 0 to 100 cm and microbial biomass C (MBC) and microbial biomass N (MBN) from 0 to 30 cm in biosolids-amended and control sites. Biosolids treatments had greater amounts of SOC and total N at all sites, and MBC and MBN were greatest at Sacramento and Solano. The largest increases in SOC were at the site that received the lowest cumulative loading rate of biosolids (Solano), where SOC content to 100 cm was 50% greater in amended soils (p<.001). Net changes in soil C stocks to 30 cm were 0.4±0.1 (Solano), -0.04±0.1 (Merced), and 0.3±0.2 (Sacramento) Mg C ha-1 yr-1 . These values change when considering deeper soil depths (0-100 cm) to 0.5±0.1 (Solano), 0.2±0.2 (Merced), and 0.216±0.2 (Sacramento) Mg C ha-1 yr-1 , reflecting differences in C stocks changes in surface and subsurface soils across sites. Rates of C storage per dry Mg of biosolids per year applied were 1±0.2 (Solano), 0.5±0.4 (Merced), and 0.04±0.1 (Sacramento). Our results suggest that local controls on soil C stabilization are more important than amendment application amount at predicting climate benefits and that accounting for soil C changes below 30 cm can provide insight for sequestering C in agroecosystems.

Carbon balance for biosolids use in commercial Douglas Fir plantations in the Pacific Northwest

Municipal biosolids are commonly used to fertilize Douglas fir (Pseudotsuga menziesii) plantations in the Pacific Northwest. An intensive soil sampling was conducted to quantify the effects of long-term biosolids application on soil carbon, nitrogen and bulk density for forest soils. This information was used to calculate a carbon balance for biosolids use in commercial tree plantations. Paired control and treated sites on two soil series (Klaus and Tokul) were included with samples collected to a 30 cm depth. A significant decrease in bulk density for two depths, but no changes in total C or N were observed in the Klaus soil. In the Tokul soil, biosolids fertilization increased C storage (total C * bulk density) for three of the four measured depths, resulting in an increase in total C from 112.8 Mg C ha−1 to 172 Mg C ha−1 for the 0–30 cm depth. A comparable increase in N was observed, with significant increases in N storage for all measured depths. The C balance considered fuel use associated with transport and application as costs. The fertilizer (N and P) content of the biosolids and changes in soil C were considered as credits. The balance showed net sequestration for both soil series. Carbon benefits for the Klaus soil totaled −0.2 Mg CO2 per Mg biosolids. Benefits in the Tokul soil totaled −5.15 Mg CO2 per Mg biosolids. Based on diameter at breast height (DBH) biosolids use also resulted in increased tree growth for both soil series. However, differences in stand age and stocking rate complicated interpretation of these results.

Residual Effects of Long-term Biosolids Application on Concentrations of Carbon, Cadmium, Copper, Lead and Zinc in Soils from Two Regions of the United States

ABSTRACT Biosolids are used to improve soil physical and chemical properties. Analysis of biosolids-amended soils from multiple regions of United States using consistent analytical methods is lacking. This Study determined long-term biosolids application on soil pH, electrical conductivity (EC), carbon, cadmium, copper, lead, and zinc concentrations in soils from two regions in United States. At one region, little difference was observed in pH and EC between biosolids-amended surface soils and control, the second region ranged from 5.46 to 7.87 and 50.2 to 402 µS cm−1 respectively. Trace metal levels at this region ranged from 0.76 to 3.79, 8.7 to 54.1, 15.2 to 53.9, and 26 to 207 mg kg−1 for Cadmium, Copper, lead and Zinc respectively; with its carbon levels ranging from 14.5 to 90.0 g kg−1. Metal levels were within the standards for residential occupation with the 40 Code of Federal Regulations (CFR) Part 503 and should not affect soil and groundwater quality.

The impact of long-term biosolids application (>100 years) on soil metal dynamics

Biosolids application to arable land is a common, and cost-effective, practice but the impact of prolonged disposal remains uncertain. We evaluated the dynamics of potentially toxic elements (PTEs) at a long-established ‘dedicated’ sewage treatment farm. Soil metal concentrations exceeded regulations governing application of biosolids to non-dedicated arable land. However, measurement of isotopic exchangeability of Ni, Cu, Zn, Cd and Pb demonstrated support for the ‘protection hypothesis’ in which biosolids constituents help immobilise potential toxic metals (PTMs). Metal concentrations in a maize crop were strongly, and almost equally, correlated with all ‘capacity-based’ and ‘intensity-based’ estimates of soil metal bioavailability. This was attributable to high correlations between soil factors controlling bioavailability (organic matter, phosphate etc.) on a site receiving a single source of PTMs. Isotopic analysis of the maize crop suggested contributions to foliar Pb from soil dust originating from neighbouring fields. There was also clear evidence of metal-specific effects of biosolids on soil metal lability. With increasing metal concentrations there was both decreasing lability of Cd and Pb, due to interaction with increasing phosphate concentrations, and increasing lability of Ni, Cu and Zn due to weaker soil binding. Such different responses to prolonged biosolids disposal to arable soil should be considered when setting regulatory limits.

Long-term biosolids application alters the composition of soil microbial groups and nutrient status in a pine plantation

Tree nutrient concentrations, root biomass, soil nitrogen (N) and phosphorus (P) contents, and enzyme activities were determined in a radiata pine plantation (Pinus radiata D. Don) on a sandy loam soil receiving 19 years of repeated biosolids applications, and the composition of soil microbial groups was assessed using phospholipid fatty acid analysis. Biosolids application significantly increased soil organic N, available P and foliar N concentration, but had no significant impact on foliar P concentration over the long term. Biosolids application improved aboveground tree growth but reduced fine-root biomass. Repeated biosolids applications also altered the composition of soil microbial community and increased the potential activities of N-acetyl-β-glucosaminidase and acid phosphomonoesterase. Changes in soil N and P availability were better explained by variations in the composition of bacterial groups than those of fungal groups. The fungi-to-bacteria ratios were not changed, but arbuscular mycorrhizal fungi were suppressed possibly due to elevated soil P caused by biosolids application. The ratios of Gram-positive to Gram-negative bacteria were significantly increased by biosolids application and were linked to increased N mineralisation rates. In addition, it is evident that variations in soil P availability and the composition of soil microbial groups following biosolids application were not significantly related to foliar P status. These findings contribute to a better understanding of the effects of biosolids application on microbial-mediated soil N and P processes.

The response of soil microbial diversity and abundance to long-term application of biosolids

The disposal of biosolids poses a major environmental and economic problem. Agricultural use is generally regarded as the best means of disposal. However, its impact on soil ecosystems remains uncertain. Biosolids can improve soil properties by supplying nutrients and increasing organic matter content but there is also a potentially detrimental effect arising from the introduction of heavy metals into soils. To assess the balance between these competing effects on soil health, we investigated soil bacterial and fungal diversity and community structure at a site that has been dedicated to the disposal of sewage sludge for over 100 years. Terminal restriction fragment length polymorphism (T-RFLP) was used to characterize the soil microbial communities. The most important contaminants at the site were Ni, Cu, Zn, Cd, and Pb. Concentrations were highly correlated and Zn concentration was adopted as a good indicator of the overall (historical) biosolids loading. A biosolids loading, equivalent to 700–1000 mg kg−1 Zn appeared to be optimal for maximum bacterial and fungal diversity. This markedly exceeds the maximum soil Zn concentration of 300 mg kg−1permitted under the current UK Sludge (use in agriculture) Regulations. Redundancy analysis (RDA) suggested that the soil microbial communities had been altered in response to the accumulation of trace metals, especially Zn, Cd, and Cu. We believe this is the first time the trade-off between positive and negative effects of long term (>100 years) biosolids disposal on soil microorganisms have been observed in the field situation.

Occurrence of Triclocarban and Triclosan in an Agro-ecosystem Following Application of Biosolids.

Triclocarban (TCC) and triclosan (TCS), two of the most commonly used antimicrobial compounds, can be introduced into ecosystems by applying wastewater treatment plant biosolids to agricultural fields. Concentrations of TCC and TCS were measured in different trophic levels within a terrestrial food web encompassing land-applied biosolids, soil, earthworms (Lumbricus), deer mice (Peromyscus maniculatus), and eggs of European starlings (Sturnus vulgaris) and American kestrels (Falco sparverius) at an experimental site amended with biosolids for the previous 7 years. The samples from this site were compared to the same types of samples from a reference (biosolids-free) agricultural site. Inter-site comparisons showed that concentrations of both antimicrobials were higher on the experimental site in the soil, earthworms, mice (livers), and European starling eggs, but not American kestrel eggs, compared to the control site. Inter-species comparisons on the experimental site indicated significantly higher TCC concentrations in mice (TCC: 12.6-33.3 ng/g) and in starling eggs (TCC: 15.4-31.4 ng/g) than in kestrel eggs (TCC: 3.6 ng/g). Nesting success of kestrels only was significantly lower on the experimental site compared to the reference site due to nest abandonment. This study demonstrates that biosolids-derived TCC and TCS are present throughout the terrestrial food web, including secondary (e.g., starlings) and tertiary (i.e., kestrels) consumers, after repeated, long-term biosolids application.

Ecological impacts of long-term application of biosolids to a radiata pine plantation

Assessment of the ecological impact of applying biosolids is important for determining both the risks and benefits. This study investigated the impact on soil physical, chemical and biological properties, tree nutrition and growth of long-term biosolids applications to a radiata pine (Pinus radiata D. Don) plantation growing on a Sandy Raw Soil in New Zealand. Biosolids were applied to the trial site every 3years from tree age 6 to 19years at three application rates: 0 (Control), 300 (Standard) and 600 (High) kg nitrogen (N) ha−1, equivalent to 0, 3 and 6Mg ha−1 of dry biosolids, respectively. Tree nutrition status and growth have been monitored annually. Soil samples were collected 13years after the first biosolids application to assess the soil properties and functioning. Both the Standard and High biosolids treatments significantly increased soil (0–50cm depth) total carbon (C), N, and phosphorus (P), Olsen P and cation exchange capacity (CEC), reduced soil pH, but had no significant effects on soil (0–20cm depth) physical properties including bulk density, total porosity and unsaturated hydraulic conductivity. The High biosolids treatment also increased concentrations of soil total cadmium (Cd), chromium (Cr), copper (Cu) and lead (Pb) at 25–50cm depth, but these concentrations were still considered very low for a soil. Ecotoxicological assessment showed no significant adverse effects of biosolids application on either the reproduction of springtails (Folsomia candida) or substrate utilisation ability of the soil microbial community, indicating no negative ecological impact of bisolids-derived heavy metals or triclosan. This study demonstrated that repeated application of biosolids to a plantation forest on a poor sandy soil could significantly improve soil fertility, tree nutrition and pine productivity. However, the long-term fate of biosolids-derived N, P and litter-retained heavy metals needs to be further monitored in the receiving environment.

Stability of soil organic matter under long-term biosolids application

Little is know on the impact of biosolids application on soil organic matter (SOM) stability, which contributes to soil C sequestration. Soil samples were collected in 2006 at plow layer from fields that received liquid and dry municipal biosolids application from 1972 to 2004 at the cumulative rate of 1416Mgha−1 in mined soil and 1072Mgha−1 in nonmined soil and control fields that received chemical fertilizer at Fulton County, western Illinois. The biosolids application increased the soil microbial biomass C (SMBC) by 5-fold in mined soil and 4-fold in nonmined soil. The biosolids-amended soils showed a high amount of basal respiration and N mineralization, but low metabolic quotient, and low rate of organic C and organic N mineralization. There was a remarkable increase in mineral-associated organic C from 6.9gkg−1 (fertilizer control) to 26.6gkg−1 (biosolids-amended) in mined soil and from 8.9gkg−1 (fertilizer control) to 23.1gkg−1 (biosolids-amended) in nonmined soil. The amorphous Fe and Al, which can improve SOM stability, were increased by 2–7 folds by the long-term biosolids application. It is evident from this study that the biosolids-modified SOM resists to decomposition more than that in the fertilizer treatment, thus long-term biosolids application could increase SOM stability.

Effect of Long-Term Application of Biosolids for Mine Land Reclamation on Groundwater Chemistry: Nutrients and Other Selected Qualities

Leaching of nitrogen (N) and phosphorus (P) to groundwater can limit the land application of fertilizer, biosolids, and other soil amendments. Groundwater quality monitoring data collected over a 34-yr period at a 1790-ha site in Fulton County, Illinois, where strip-mined land was reclaimed with biosolids, were used to evaluate long-term impacts of biosolids on groundwater N, P, and other parameters. Seven strip-mined fields repeatedly treated with biosolids at 801 to 1815 Mg ha cumulative rate (equivalent to 24-55 dry Mg ha yr) between 1972 and 2004 were compared with another seven fields treated annually with chemical fertilizer at agronomic rates. Groundwater from wells installed in each of the fields and two public wells that served as background (reference) were sampled for 35 yr, monthly between 1972 and 1986 and quarterly between 1987 and 2006. Data show greater chloride (Cl), sulfate (SO) and electrical conductivity (EC) of groundwater from wells in biosolids fields than those in fertilizer fields. Also, groundwater nitrate N (NO-N) concentrations were greater in biosolids-amended fields than in fertilizer fields, but below regulatory limit of 10 mg L in Illinois Part 620 regulation. Conversely, groundwater P concentrations were consistently lower in biosolids than in chemical fertilizer wells throughout the 35-yr monitoring period. The study demonstrates that the repeated application of biosolids, even at higher than agronomic rate, would cause only minor nitrate increase and no P increase in groundwater.

Effects of Long‐Term Application of Biosolids for Mine Land Reclamation on Groundwater Chemistry: Trace Metals

Data collected for 35 yr from a 1790-ha strip mine reclamation site in Fulton County, Illinois, where biosolids were applied from 1972 to 2004, were used to evaluate the impacts of long-term biosolids application on metal concentrations in groundwater. Groundwater samples were collected between 1972 and 2006 from wells installed in seven strip-mined fields treated with biosolids at cumulative loading rates of 801 to 1815 dry Mg ha and from another seven fields (also strip mined) treated with mineral fertilizer. Samples were collected monthly between 1972 and 1986 and quarterly between 1987 and 2004 and were analyzed for total metals. The concentrations of metals in groundwater were generally below regulatory limits. Lead, Cd, Cu, Cr, Ni, and Hg concentrations in groundwater were similar for the biosolids-amended and fertilizer-treated sites across all sampling intervals. Zinc concentration was increased by biosolids application only for samples collected before the 1993 promulgation of the USEPA 40 CFR Part 503 rule. Iron and Mn were the only metals that were consistently increased after biosolids application; however, Mn concentrations did not exceed the 10 mg L regulatory limits. Zinc, Cu, Cd, Pb, Fe, Al, and Mn concentrations in groundwater decreased with time, coupled with the change from pre-part 503 to post-Part 503 biosolids. The concentrations of other metals, including Ni, Cr, and Hg, did not increase in groundwater with the prolonged biosolids application. The study suggests that the long-term application of biosolids at high loading rates does not result in trace metal pollution of groundwater.

Long-term impact of 19 years’ farmyard manure or sewage sludge application on the structure, diversity and density of the protocatechuate-degrading bacterial community

Impact of long-term biosolids application on soil-living micro-organisms key players of ecosystemic services is scarcely reported. Here, the impact of the 19 year-long application of farmyard manure (FM) and sewage sludge (SS) organic fertilisation regimes on the protocatechuate-degrading bacterial (pca) community was estimated by comparison to a mineral fertilisation regime (U). The structure, diversity and density of the pca community were determined using pcaH, a gene encoding the protocatechuate 3,4-dioxygenase. Ten years after the last application, the structure of the pca community in soils amended with SS100 (100t/ha/2 years) and to a lesser extent with FM (10t/ha/year) was still different from that in U treatment. pcaH amplicons from all treatments were cloned, screened by RFLP and sequenced. The diversity was studied by Shannon–Weiner and Simpson indexes and by rarefaction curves estimated from pcaH library analyses, showing that the pcaH community was impacted in SS10 and SS100, compared to U. The sequencing of pcaH amplicons supports the results from the RFLP analysis. Quantification of the abundance of the pca community by qPCR assays showed a significant increase in SS100 in comparison to U, FM and SS10. Overall, 10 years after the last application, the impact of 19 years’ organic fertilisation on the pcaH community was still traceable, highlighting the lack of resilience of this functional community.

Micronutrient Availability as Affected by the Long-Term Application of Phosphorus Fertilizer and Organic Amendments

Micronutrient availability is important to crop production and can be affected by long-term application of P and organic amendments. This study was conducted to determine the effects of the long-term application of different sources and rates of P application on extractable [DTPA-sorbitol (diethylenetriamine-pentaacetic acid and sorbitol] micronutrients. Soil extractable and total micronutrients (B, Cu, Fe, Mn, Mo, and Zn), pH, and organic matter content were determined from sites across Oklahoma that had received long-term applications of beef manure, swine effluent, biosolids, or commercial fertilizer. Three continuous inorganic P experiments that had been conducted for 36 to 39 yr and three organic P experiments that had been conducted for 11 to 12 yr were evaluated. The long-term application of inorganic P had little effect on micronutrient availability. However, long-term application of biosolids significantly (p < 0.05) increased extractable Cu, Fe, Mo, and Zn in soil, while long-term application of beef manure significantly increased all the micronutrients evaluated. Similarly, the long-term application of swine effluent significantly increased extractable B, Cu, Mo, and Zn in soil. The addition of micronutrients from organic amendments increased micronutrient availability, while long-term inorganic P application had little effect. The result of our study indicates organic amendments make an ideal fertilizer source for areas with micronutrient deficiencies.