Longline Method Research Articles

Growth Response of Kappaphycus Alvarezii at Different Depth in the Waters of Batu Bao, East Nusa Tenggara

ABSTRACT Seaweed is one of the important fisheries commodities with significant economic value. Kappaphycus alvarezii, commonly known as Eucheuma cottoni in the market, is a type of seaweed that produces kappa carrageenan. This research aims to determine the effective planting depth for the growth, morphological and histological changes, and the environmental parameters influencing the growth of K. alvarezii. The cultivation method used in this research is the longline method, and it employs a Completely Randomized Design consisting of four treatments: 15cm, 30cm, 45cm, and 60cm, with each treatment replicated five times. The data were analyzed using analysis of variance, and if significant differences were found, Duncan's post hoc test was conducted. The research results indicate that an increase in planting depth has a significant impact on specific growth rate (SGR) and average growth rate (AGR). Deeper planting depths result in slower growth. The best depth for the growth, morphology, and histology of K. alvarezii during the study was observed at a depth of 15 cm. Environmental parameters such as temperature, salinity, water flow rate, water clarity, and pH have a positive influence on the growth of K. alvarezii.

Optimization of Long Line Management in Seaweed Farming: Strategies for Increasing Farmers' Group Income in Pinrang Regency, Indonesia

This research aims to explore the role of the long-line method in increasing the income of seaweed farmers in Pinrang Regency, South Sulawesi, Indonesia, while examining the challenges they face and the strategies they employ to overcome them. The study employs a qualitative case study approach, involving 15 seaweed farmers from various coastal villages within Pinrang Regency, representing diverse demographic backgrounds and experiences. Data collection was conducted through semi-structured interviews, allowing for an in-depth understanding of the participants' farming practices and challenges. The findings indicate that seaweed farmers increase their income by optimizing resources, collaborating within farmer groups, and adapting to environmental and market changes. Strategies such as space optimization, material reuse, and knowledge sharing among farming communities significantly enhance productivity and sustainability. Furthermore, farmers employ adaptive techniques to mitigate environmental risks and market volatility, including using durable materials and processing seaweed into higher-value products. The implications of these findings are crucial for policymakers and development organizations, as they highlight the potential of sustainable farming practices in improving the livelihoods of coastal communities. Policymakers are encouraged to develop support programs that promote resource optimization, foster community-driven farming networks, and train farmers in adaptive strategies. However, the study's limitations, including the small sample size and reliance on self-reported data, may affect the generalizability of the results. Future research should incorporate larger, more diverse samples and objective measures to enhance the robustness of the findings, and explore the long-term impacts of the long-line method on income and sustainability.

Growth Performances and Productivities of Kappaphycus alvarezii and Eucheuma spinosum Cultivated with Long-line Method in PT. Sea Six Energy Indonesia

Indonesia is the second largest producer of seaweed in the world. Different cultivated seaweed commodities may have different production results. This study aimed to determine the effect of cultivation time and different types of seaweed on total weight and Specific Growth Rate (SGR) of Kappaphycus alvarezii and Eucheuma spinosum cultivated with the long-line method. The study was conducted in one cultivation area, PT. Sea Six Energy, Bali, Indonesia. Data collection was carried out through observations in 2 sites. Every site contained 3 square frame seaweed Kappaphycus alvarezii and Eucheuma spinosum. The sample used in this study was one thallus of seaweed taken randomly from 3 different ris ropes in each square frame. The average weight of thallus, total weight, and specific growth rate were performed in this study. After 51 days, the total weight of K. alvarezii was 1348.5 kg. The total weight of E. spinosum was 1398.7 kg. Both, K. alvarezii (8.4 ± 0.34 %.day-1) and E. spinosum (8.3 ± 0.02 %.day-1) showed higher Spesific Growth Rate (SGR) in the first 2 weeks of planting but not in the last 3 weeks of cultivation. There was no difference in productivity between K. alvarezii and E. spinosum cultivated using the long-line method. However, this research showed that the SGR value decreased as cultivation time increased.

Pertumbuhan tiram mutiara (Pinctada maxima) menggunakan metode karambah apung dan longline

Pearl oyster cultivation is a growing industry in various countries. The growth and quality of pearls produced by oysters is greatly influenced by the cultivation method used. This research aims to compare the growth of pearl oysters (Pinctada maxima) cultivated using the floating cage and longline methods. Different cultivation methods can influence the growth of pearl oysters and the surrounding environmental conditions. Statistical analysis was conducted on sample size, mean values, standard deviation, and standard error of the mean for both cultivation methods. The results indicate no significant difference in the growth of pearl oysters between the floating cage and longline methods. However, biofouling and other disrupting organisms can affect the growth of pearl oysters in the floating cage method. The study also highlights the importance of environmental factors such as water quality, temperature, and food availability in influencing the growth of pearl oysters. Thus, this research provides valuable insights for the pearl oyster cultivation industry in selecting the optimal cultivation method to enhance productivity and the quality of the pearls produced.

Long-lines for research monitoring and efficient population regulation of an invasive apex predator, European catfish (Silurus glanis)

European catfish is a large-bodied apex predator, a key species in native areas, but invasive in others where it negatively impacts local aquatic fauna necessitates catfish regulation. However, traditional ichthyological methods face challenges in capturing it. The study presents a detailed description of the efficient long-line method, refined through 48 sampling campaigns across twelve European water bodies. This method proves cost-effective and technically undemanding, requiring an average of 5.6 bait fish to catch one European catfish per day. The long-lines outperform other techniques, with the highest Biomass per unit effort (BPUE) of 6.205 kg of catfish per man-hour and minimal by-catch (0.276 kg per man-hour). In contrast, fyke nets, the second most efficient method, achieve a BPUE of 0.621 kg of catfish per man-hour with 3.953 kg of by-catch per man-hour. To optimize long-line catches, a 15 m distance between branch lines and regular relocation is recommended. Live fish is the most effective bait with no significant differences observed among species. However, earthworms, a less controversial alternative, are also efficient, especially for smaller catfish. Our recapture approach using various ichthyological methods revealed no hook avoidance behavior by catfish after a previous catch or avoidance by a certain part of the population. The long-line method is suitable for population regulation, scientific research, and conservation efforts and is the most effective means of capturing live European catfish.

Application of the Longline Method of Seaweed Turbinaria ornata Cultivation through Spacing Treatment

Application of the Longline Method of Seaweed Turbinaria ornata Cultivation through Spacing Treatment

Analyzing the potential for Padina gymnospora cultivation in the coastal waters of St. Martin’s Island, Bay of Bengal, Bangladesh: Determining the fluctuations of physicochemical property across seasons and locations

Cultivating seaweed presents an opportunity to enhance the livelihoods of the inhabitants of St. Martin's Island inhabitants. This research investigates the suitability of the island's coastal waters for seaweed cultivation, taking into account variations in physicochemical factors in different seasons. The water temperature and salinity at various locations ranged from 24.22ºC to 27.64ºC and 23.8 to 33.76 psu, respectively. pH remained relatively constant throughout the year, fluctuating between 7.71 and 8.22. Dissolved oxygen levels were highest during the dry, cooler winter season (6.47±0.21 ppm) and lowest during the monsoon season (4.96±0.09 ppm). Electrical conductivity (EC) ranged from 39.00 mS/cm to 53.46 mS/cm, while total dissolved solids (TDS) varied between 20.35 and 27.90 g/l. Water clarity was at its peak during the dry, cooler winter season, averaging 4.14 meters. This investigation confirms the feasibility of year-round seaweed cultivation in the coastal waters around St. Martin's Island. In our study comparing cultivation methods for Padina gymnospora during the dry, cool winter season, the long-line approach resulted in a higher daily weight gain compared to the floating net method. This trend extended to growth rates by length, with the long-line method demonstrating a 5.09% daily growth and the floating net method showing 4.46%. However, net biomass production was 289.32 g/m² for the long-line method and 381.28 g/m² for the floating net method. Furthermore, the Cost-Benefit Ratio values for the long-line and floating net methods were 2.57 and 3.27, respectively. These findings affirm the viability of cultivating Padina gymnospora in coastal waters using the floating net method. Biodivers. Conserv. Bioresour. Manag. 2024, 10(1): 1-14

Strain selection of red algae Kappaphycus alvarezii (Doty) Doty farmed using different methods in Tablolong Waters, Kupang Regency-East Nusa Tenggara Province

The main problem is facing in farming red algae Kappaphycus spp. in Kupang Regency are decreasing quality of seeds and the infection of ice-ice disease. This research aimed to analyze the growth of different strains of K. alvarezii farmed using different methods, and analyze which strains of K. alvareziii have high resistance to ice-ice disease. This research was carried on August to November 2020 in Tablolong waters, Kupang Regency. The strains used were Sacol from Sulamu and Semau, Tambalang (tissue culture) from Rote Island, and Cottonii strain from Pasir Panjang. The farming methods used were the Longline and Off-Bottom. The main data taken were wet weight and incidence as well as infection intensity of the disease. The study found that growth rates varied based on the strains and farming methods. Based on the strain, the Sacol strain from Sulamu gave a higher growth than the others. This strain grew and reached an average weight of 1087.5 grams at week 7; with an average specific growth of 4.87% per day. Based on the method, the longline method gave better growth compared to the off-bottom method. Regarding ice-ice disease, the Sacol strain from Sulamu gave higher resistance to disease compared to the other strains, with an average incidence of 27.26% and an infection intensity of 2.81%. The longline method provides higher disease resistance than the off-bottom method.

Transfer Teknologi Pengayaan Bibit Rumput Laut Yang Dibudidayakan Dengan Keramba Jaring Apung

Inappropriate cultivation methods and a decrease in the quality of seaweed seeds are some of the obstacles in seaweed production. Technology transfer activities through community service programs aim to provide learning to seaweed farmers on how to enrich seaweed seeds with bionic fertilizer and then cultivate them using the floating net cage method. The activity was carried out on August 24-25, 2022 at the Main Base Command Headquarters of the Indonesian Navy VII Kupang. The method used was a participatory empowerment approach involving seaweed farming communities with counseling and dissemination. Seaweed cultivation with the floating net cage method showed higher absolute growth, relative growth and daily growth rate compared to the longline method, which were 11.91 ± 0.69 g day-1, 0.032 ± 0.002 g g-1 day-1 and 3.14 ± 0.17% day -1, respectively. While in the longline method, it was 9.48±1.81 g day-1, 0.027±0.004 g g-1 day-1, and 2.65±0.43% day -1, respectively.

PERBEDAAN JUMLAH IKAT BIBIT TERHADAP PERTUMBUHAN RUMPUT LAUT (Kappaphycus alvarezii) MENGGUNAKAN METODE LONG LINE PANTAI AMAL KOTA TARAKAN

Seaweed is one of the fisheries commodities that is widely cultivated in Indonesia. The cultivation of seaweed (Kappaphycus alvarezii) in the Coastal Waters of Amal Baru, Tarakan City, is traditionally done using the longline method. The gillnet method is the most used method by seaweed farmers because it is flexible in choosing locations and relatively inexpensive. The research aims to determine the difference in the number of seaweed seedlings on the absolute and specific growth rates. This study used a Completely Randomized Design (CRD) with 4 treatments and 3 replications. Treatment P1 (1 bundle), P2 (2 bundles), P3 (3 bundles), and P4 (4 bundles). The results showed that the growth of seaweed seedlings with different numbers of bundles influenced absolute and specific growth rates. The best treatment was found in treatment P4 with an average absolute growth rate of 80.00% and an average specific growth rate of 100%.

Evaluation of Different Culture Methods on The Growth Performance of Seaweed (Kappaphycus striatum) in Pelakak Village Waters Lingga District

The seaweed culture activities are relatively new and only few people perform them in Lingga District. Specifically, Kappaphycus striatum has recently been cultured for the last three culture periods. This condition causes the seaweed culturists have no appropriate data associated with a proper culture method, following the water characteristics around the culture area. A study regarding the different culture methods on the growth performance of K. striatum in Pelakak Village waters, Lingga District, Riau Islands has never been reported. This study aimed to determine the effect of different culture methods on the growth performance of Kappaphycus striatum seaweed. This study used a completely randomized block design with three treatments and three replications, namely bottom method (P1), off-bottom (P2), and longline method (P3). There were 351 seaweed seeds with 100 g weight per each seed, that are placed in nine culture plots. The culture plot was constructed as a 4 m × 1.5 m square with wooden stakes at both ends and polyethylene ropes installed at a distance of 0.5 m. Each rope stretch was tied at 0.3 m distance for seed planting. Parameters observed in this study were absolute growth rate, specific growth rate, and production rate of the seaweed. The results showed that the absolute growth rate, specific growth rate, and production rate were affected by the culture method (p<0.05). The seaweed group reared in a longline method (P3) obtained the best results, compared to other methods, at 2.34% per day for specific growth rate and 245.7 g/m2 for production rate. Therefore, the longline method can be applied in the Pelakak Village waters, Lingga District.

Regeneration of Kappaphycus alvarezii seaweed resulting from the transformation of the PaCs gene (Citrate Synthase) to increase resistance to environmental congestion in floating net cages in Pangkep water

The PaCs (Citrate Synthase) gene of the seaweed K. alvarezii has effectively undergone in vitro transformation. To improve the survival of the transformation of the Citrate Synthase seaweed gene, it is carried out: (1) Regeneration of the results of transformation in culture chamber in the laboratory; (2) Acclimatization in green net (MS 100 μm) size 50x50x50 cm, density 200 explants maintained for 2 weeks, transferred to the blue net with a size of 50x50x50 cm (MS 200 μm) density 200 explants maintained for 4 weeks, then maintenance by the longline method using a double rope with a distance of 15 Cm on floating net cages. At intervals of two weeks, measurements of size, length, and water quality are made. Analysis of PaCs gene integration with PCR using Primary PaCS-F and PaCS-R. The results showed that the long DGR in the culture chamber was 0.11% to 0.18%/day, weight growth rate 0.62-1.30%/day, Acclimatization on green nets indicates a long growth rate of 2.28-2.3%/day, weight grow rate of 0.62 to 1.3% / day, and on blue nets, increases long grow rate 3-5%/day and weight 1.15-2.18%/day. Seaweed enlargement using the longline method shows a long growth rate of 5.3-9%/day and weight growth range from 1,23-3.2%/day. The band was located in the CaMV 35S vector at a place corresponding to the 1630 bp PaCS-F and PaCS-R promoter segments, according to the results of the integration of the Citrate Synthase gene in seaweed. Based on these findings, the seaweed K. alvarezii has successfully received the PaCS gene insertion.

Raft and Longline Culture of Green Mussel, Perna viridis, in Cañas Bay, Iloilo, Philippines

Grow-out methods like the raft and longline have been utilized by various countries in growing green mussel, Perna viridis. In the Philippines, the stake method using bamboo poles is still widely used, which is believed to cause siltation that negatively affects green mussel production. In this study, the feasibility of raft and longline methods was determined based on mussel growth and the condition index (CI). Environmental conditions in Cañas Bay, Iloilo were also presented. Results showed that there was no significant difference in mussel growth except for final weight, where mussels in the raft were found to be significantly heavier than those in the longline. Meanwhile, the CI of mussels did not significantly differ between the two methods. Physico-chemical parameters of the water in Cañas Bay were found to be suitable for growing green mussels. Phytoplankton was composed of diatoms (86%), dinoflagellates (6%), green algae (5%), and blue-green algae (3%). This study showed the feasibility of using suspended methods like raft and longline in areas where environmental conditions favor mussel growth.

The Effect of Seed Weight on Growth of Seaweeds Kappaphycus alvarezii in Integrated Marine Aquaculture of Ekas Bay, East Lombok Regency

Integrated marine aquaculture or known as Integrated Multi-Tropical Aquaculture (IMTA) is an alternative development in aquaculture activities under the concept of environmental conservation. There are several important aspects in supporting the success of seaweed cultivation including the use of the right weight of seeds at the beginning of cultivation activities. The purpose of this study was to analyze the effect of seedling weight on the growth of Kappaphycus Alvarezii seaweed cultivated in integrated cultivation areas and to determine the best use of seed weight. This study used a completely randomized design (CRD) consisting of 4 treatments and 3 replications to obtain 12 experimental units. The treatments used were the initial seedling weights of 50 g, 100 g, 150 g, and 200 g. The results showed that the effect of different seed weights on the integrated cultivation system with the longline method showed that the absolute average growth obtained ranged from 57.67 g to - 149.00 g while the average specific growth rate of seaweed ranged from 1.28%/day to - 3 ,31%/day and the best chlorophyll-a was found in the 50 g treatment with a value range of 0.628 mg/L to - 1.520 mg/L. It can be concluded that the use of seed weight gave a significantly different effect on the absolute growth and specific growth rate of Kappaphycus Alvarezii seaweed that was cultivated in an integrated manner and the use of the best seed weight with the best weight was 50 g.

Supply Chain Analysis of Green Mussel Business in Umbul Asem Village Bandar Lampung

Umbul Asem village is a coastal area has a fairly high potential, especially the farming of green mussels. However, the prospect of green mussel farming which is quite large is still not realized by all fishermen so it is necessary to conduct research to determine the green mussel supply chain and see what opportunities from its business so that it can be developed. This is a descriptive research which is conducted in field and desk research. The results indicate the green mussel supply chain starts from farmers to distributors then to retailers and to end consumers, while supply chain players manage relationships between supply chain from upstream to downstream and vice versa well. Some of the green mussel business opportunities that are visible include selecting the Longline method to minimize costs and increase profits also suitable for high wave waters, farming is simple and does not require special treatment, lots of green mussel seeds are available in the waters, the profit from one longline is around Rp. 691,667, the location is close to PPI Lempasing (3 km in 8 minutes). In addition to developing the green mussel business, it is necessary to conduct a study that is in line with the government's efforts in formulating a strategy for developing the fishing industry in Bandar Lampung.

Evaluation of Water Suitability for Sustainable Seaweed (Kappaphycus Alvarezii) Cultivation to Support Science Technopark in North Kalimantan

Tarakan dry seaweed production increased during 2012-2018 for the cultivation of Kappaphycus alvarezii with the longline planting method. This study aims to assess the quality of the waters and their suitability for seaweed cultivation on the coast of Tarakan Island. The environmental parameters of water quality measured were chlorophyll-a, water temperature, salinity, pH, DO, TDS, turbidity, nitrate, phosphate, water depth, current velocity, protection, research location, and distance between settlements. The study was conducted with in-situ and ex-situ measurements based on APHA (2012). Water samples were taken using the multi-parameter Horiba U51. chlorophyll-a was analyzed using spectrophotometric methods, nitrate levels were analyzed using SNI 06-6989.79-2011, while phosphates were analyzed using SNI 06-6989.31-2005. The suitability of the waters is divided into 4 classes, S1 (very suitable), S2 (suitable), S3 (marginally suitable), and N (not suitable). The results showed that the coast of Tarakan Island has 3 categories, namely marginally suitable (S3) 13.20%, suitable (S2) 86.50%, and very suitable (S1) 0.30%. The existing condition of the coastal waters of Tarakan Island supports the cultivation of K. alvarezii seaweed. Tarakan Island coastal existing condition as an areal support seaweed cultivation K. alvarezii with the potential to be developed land area of 33896.73 ha.

Pengaruh Metode Budidaya dan Asal Bibit terhadap Pertumbuhan Gracilaria verrucosa Yang Dibudidayakan Di Tambak Desa Kaliwungu, Kabupaten Brebes

Gracilaria verrucosa is one of the seaweeds with good economic value that can be cultivated in ponds. The demand for Gracilaria is increasing as an ingredient for food, beverage, cosmetic, pharmaceutical manufacturing industries. The factors that determine the success of Gracilaria culture: cultivation methods include the seeds quality and source.This study aims to determine the effect of different Gracilaria seeds sources and its culture methods on its growth, to determine the interaction between methods and seed sources. This study aims to determine the effect of different Gracilaria seeds sources and its culture methods on its growth, to determine the interaction between methods and seed sources The research was conducted in October-November 2020 in Kaliwlingi Village, Brebes District, Brebes Regency, Central Java. The experimental used was factorial design with 2 factors: Factor 1: seed sources (A): A1 = Seed from Semarang; A2 = seed from Brebes; A3 seeds from Demak. Factor 2: cultivation method (B): B1 = longline; B2 = off-bottom; B3: broadcast. So there were 9 treatment combinations: A1B1, A2B1, A3B1; A2B1, A2B2, A2B3; A3B1, A3B2, A3B3, each treatment with 3 replications. The data collected include: growth of gracilaria during 60 days. The Specific Growth Rate (SGR) analyzes using ANOVA. The results showed the origin of seedlings and cultivation methods have a very real effect (P<0.05) on SGR Gracilaria. The highest SGR was at treatment A1B1 (Semarang seeds longline method) = 1.84±0.17%/day, and the lowest was treatment A3B3 (Demak seedlings broadcast method) of 0.25±0.13%/day. The water quality measurement results obtained indicate that the water quality at the research location is in a tolerable range for the growth of seaweed. Keywords: Gracilaria, seed source, longline, broadcast, off-bottom.

Pertumbuhan rumput laut Kappaphycus alvarezii yang menggunakan tali ris senar secara vertikultur

The purpose of the study was to determine the growth of Kappaphycus alvarezii seaweed which was cultivated vertically using strings. The method is the use of the longline method vertically using strings. The experimental design was a completely randomized design with 3 treatments, namely different depths, and each treatment carried out 20 repetitions. Cultivation was carried out for 45 days, where seaweed growth measurements were carried out every 14 days. The highest absolute growth was in treatment A (2,675 grams) followed by treatment B (2,660 grams), treatment C (2480 grams). The highest daily growth rate was treatment B (6.79%) followed by treatment A (6.77%) and treatment C (6.55%). Furthermore, the results of the analysis showed that the absolute growth in treatments A and B was different from treatment C, as well as the daily growth rate which gave a very significant effect, where treatments A and B were very different from treatment C.

Effect of different seedling weights on Kappaphycus alvarezii growth

Seaweed K. alvarezii is one of leading commodities in aquaculture because this seaweed species produces carrageenan which has high economic value. This study aimed to determine effect of different seedling weights on the growth of seaweed K. alvarezii, and to determine which seedling weight had the best effect on seaweed growth. Planting process was carried out using longline method. Size of longline construction used was 10 m x 20 m, with a length of 20 m and a distance of 1 m between each ropes. Each rope line contained about 66 clumps of seedlings with a distance between clumps of 15 cm. Thallus weight used was 30 gr, 50 gr, 70 gr for each treatment. The highest mean value of absolute weight growth of seaweed K. alvarezii was found in treatment C (thallus weight 70 gr) with an average value of 99.27 gr, followed by treatment A (thallus weight 30 gr) of 51.95 gr and the lowest value found in treatment B (thallus weight 50 gr) of 51.65 gr.

Acclimatization of transformed seaweed Kappaphycus alvarezii carrying lysozyme gene in culture flask and the cultivation in floating net cage in Pangkep coastal

The in vitro transformation of the lysozyme gene in seaweed K. alvarezii has been successfully executed to increase the viability against ice-ice disease. There were two major stages in this research; (1) transformation of lysozyme gene in seaweed K. alvarezii which was carried out on laboratory scale and the cultivation of gene-transformed explants in the culture flask stored in “culture chamber”; (2) the acclimatization in floating net cages of green nets (mesh size of 1 mm) with cage size of 50 x 50 x 50 cm, the population density of 200 explants and cultivated for two weeks. The explants were then transferred to blue nets (mesh size of 2 mm) with a cage size of 50 x 50 x 50 cm for four weeks of rearing. The plants were then enlarged using a long-line method in the floating net cage, by tying the seaweed using a double line with a gap of 15 cm each. The measurement of weight, bud lengths, and water quality was carried out within 2 weeks. The result shows that the daily growth rate of the transformed seaweed during the regeneration stage in the culture flask was around 0.33-0.4%/day, meanwhile during the acclimatization stage in the green nets the was 0.65-1.6%/day, and even more, increased during the acclimatization stage in the blue nets with DGR of 2.28-2.3%/day. During the enlargement stage in the floating net cages, the lysozyme-transformed seaweed showed an even higher DGR with a value of 3.2-8.2%/day. The results of the integration of the lysozyme gene in seaweed were indicated by the presence of a 670 bp of amplification products, that is the same total length of the 35 S-F promoter fragments and Nos T-R in the expression vector. Based on these results, the lysozyme gene was successfully transformed in K. alvarezii seaweed.