Secondary carbonate accumulations are very common in arid and semi-arid soils but are often poorly described and classified. This is the case for queras that are observed in several loess-palaeosol sequences of the Ebro Basin. Micromorphologically, queras are complex pedogenic features basically consisting of infillings of calcified root cells in channels, surrounded by decarbonated hypocoatings. They apparently result from biologically mediated calcification/decalcification processes at a microscale. The aim of this study is to systematically characterize biocalcifications found in dated loess-palaeosol sequences, to determine the main factors of formation, and to discuss their possible use as a palaeoenvironmental proxy. Different queras show a similar number of biosparite layers (four to five) around a central channel, and a decarbonated hypocoating around the queras. This supports the hypothesis that queras originate from the calcification of root tips and are related to the acidification of surrounding soil for nutrient absorption. Under cathodoluminescence microscopy, biosparite has a different behaviour than non-biological calcite crystals: the latter appears reddish while biosparite remains black. The queras, with ages corresponding to Marine Isotope Stages 1–3, are younger than that of the loess where they are found in. The stable isotope values likely correspond to C3 plants, and the estimated temperatures of formation reflect a Mediterranean temperate climate. This suggests that the biocalcifications developed during short moist seasons amidst arid periods of the Quaternary.