Failure to apply lockout procedures for the control of hazardous energies is one of the main causes of machinery-related fatal and serious injuries in North America. The absence of audits of lockout or the lack of proper tools for auditing lockout is prevalent, and thus the application of lockout is often not fully in compliance with standards and regulations. A self-audit tool for the application of lockout procedures for machinery was developed on the basis of the current standards and regulations, and previous research. The tool was then tested for content validity through experts’ opinions and qualitative feedback from six organizations in the province of Quebec in Canada. The developed audit tool defines the actual procedures to audit, as well as the surrounding conditions that are needed and the prerequisites based on standards, regulations, and findings from previous research. The results showed that the tool displayed a high content validity index and demonstrated that the usability, applicability, and comprehensiveness of the tool were adequate. This self-audit tool helps organizations monitor the application of lockout on machinery for the safety of workers and to ensure that the actual practice of controlling hazardous energy is in compliance with relevant standards and regulations.