Locations In South America Research Articles

Application of herbivore ecometrics to reconstruct Neogene terrestrial palaeoenvironments in Falcón, Venezuela

Despite its important position close to the Isthmus of Panama, and abundance of fossil localities, the palaeoenvironment of the Falcón Basin in northwestern Venezuela has been understudied. The basin includes a sequence containing 6 terrestrial fossil assemblages, of which 5 are well-sampled enough for palaeoenvironmental reconstruction. Here we use these assemblages as a test case for taxon-free approaches in the Neotropics, that have been used in studies elsewhere, including dental functional traits, body mass and mesowear. Mesowear reveals browsing and grazing diets from the Late Miocene to the Late Pleistocene, and though the toxodontids (the most abundant mammalian herbivores) show a consistent browsing signal, other approaches support an environment which was largely more open than previously suggested. We find limited evidence for any dramatic climatic shift, instead finding more gradual changes in precipitation and tree cover. Ultimately, we find enough consistency in our results with those of other proxies to suggest that the methods employed here could potentially be applied to other localities in South America.

All-cause, cardiovascular, and respiratory mortality and wildfire-related ozone: a multicountry two-stage time series analysis

Wildfire activity is an important source of tropospheric ozone (O3) pollution. However, no study to date has systematically examined the associations of wildfire-related O3 exposure with mortality globally. We did a multicountry two-stage time series analysis. From the Multi-City Multi-Country (MCC) Collaborative Research Network, data on daily all-cause, cardiovascular, and respiratory deaths were obtained from 749 locations in 43 countries or areas, representing overlapping periods from Jan 1, 2000, to Dec 31, 2016. We estimated the daily concentration of wildfire-related O3 in study locations using a chemical transport model, and then calibrated and downscaled O3 estimates to a resolution of 0·25° × 0·25° (approximately 28 km2 at the equator). Using a random-effects meta-analysis, we examined the associations of short-term wildfire-related O3 exposure (lag period of 0-2 days) with daily mortality, first at the location level and then pooled at the country, regional, and global levels. Annual excess mortality fraction in each location attributable to wildfire-related O3 was calculated with pooled effect estimates and used to obtain excess mortality fractions at country, regional, and global levels. Between 2000 and 2016, the highest maximum daily wildfire-related O3 concentrations (≥30 μg/m3) were observed in locations in South America, central America, and southeastern Asia, and the country of South Africa. Across all locations, an increase of 1 μg/m3 in the mean daily concentration of wildfire-related O3 during lag 0-2 days was associated with increases of 0·55% (95% CI 0·29 to 0·80) in daily all-cause mortality, 0·44% (-0·10 to 0·99) in daily cardiovascular mortality, and 0·82% (0·18 to 1·47) in daily respiratory mortality. The associations of daily mortality rates with wildfire-related O3 exposure showed substantial geographical heterogeneity at the country and regional levels. Across all locations, estimated annual excess mortality fractions of 0·58% (95% CI 0·31 to 0·85; 31 606 deaths [95% CI 17 038 to 46 027]) for all-cause mortality, 0·41% (-0·10 to 0·91; 5249 [-1244 to 11 620]) for cardiovascular mortality, and 0·86% (0·18 to 1·51; 4657 [999 to 8206]) for respiratory mortality were attributable to short-term exposure to wildfire-related O3. In this study, we observed an increase in all-cause and respiratory mortality associated with short-term wildfire-related O3 exposure. Effective risk and smoke management strategies should be implemented to protect the public from the impacts of wildfires. Australian Research Council and the Australian National Health and Medical Research Council.

Neogene–Quaternary tectonic, eustatic and climatic events shaped the evolution of a South American treefrog

AbstractAimUnderstanding how past geological and climatic events could have generated intraspecific genetic variation at different temporal and spatial scales in the Neotropics requires a model encompassing distinct ecoregions. Because Anura species have their evolutionary history affected by climate and environmental changing, it is a suitable model to investigate the signatures of Neotropical historical events in species populations. Here we used a treefrog species to test hypotheses associated with tectonic, eustatic and climatic events along with isolation‐by‐environment and isolation‐by‐distance spatial patterns in genetic diversity.TaxonScinax squalirostris (Lutz, 1925), Anura: Hylidae.LocationAtlantic Forest, Cerrado, Chaco, Uruguayan Savanna and Pampas ecoregions in South America.MethodsWe sequenced nuclear and mitochondrial data from 219 individuals of S. squalirostris sampled at 26 locations in South America. We performed statistical phylogeographic analyses and reconstruction of spatiotemporal dispersal dynamics. Ecological niche modelling was conducted to predict spatial changes in environmental suitability from the Last Glacial Maximum to the Holocene and present day.ResultsWe recovered a significant phylogeographic break roughly dated back to the Miocene (~7.6 Ma), coinciding with the retraction of marine incursions in the Paraná basin. Most dispersal events occurred in cooler periods of the Pliocene–Pleistocene transition. The spatial pattern in genetic diversity was not correlated with geographical distance or environmental variables.Main ConclusionsScinax squalirostris had a quasi‐stable distribution through time. Neogene–Quaternary tectonic, eustatic and climatic events drove the demographic history and dispersal dynamics of species, with a phylogeographical break dated to the Miocene coinciding with retractions of marine incursions in the Paraná basin. Lineage dispersal spread from the southern Atlantic Forest and retrieved gene flow signals congruent with the cold periods of the Pliocene and Pleistocene.

Revision of Gypogyna Simon, 1900 (Araneae: Salticidae).

Gypogyna Simon, 1900 is revised. Three species are recognized and diagnosed. The type species, Gypogyna forceps Simon, 1900, is redescribed and recorded from several localities in South America. Gypogyna amazonica Ruiz sp. nov. is described based on a male from Manaus, Brazil. Gypogyna mexicana Ruiz Bustamante sp. nov. is described based on specimens of both sexes from the state of Jalisco, Mexico. In addition, some comments on cheliceral dentiton and genital morphology are discussed.

Development of a multi-species SNP array for serrasalmid fish Colossoma macropomum and Piaractus mesopotamicus

Scarce genomic resources have limited the development of breeding programs for serrasalmid fish Colossoma macropomum (tambaqui) and Piaractus mesopotamicus (pacu), the key native freshwater fish species produced in South America. The main objectives of this study were to design a dense SNP array for this fish group and to validate its performance on farmed populations from several locations in South America. Using multiple approaches based on different populations of tambaqui and pacu, a final list of 29,575 and 29,612 putative SNPs was selected, respectively, to print an Axiom AFFYMETRIX (THERMOFISHER) SerraSNP array. After validation, 74.17% (n = 21,963) and 71.25% (n = 21,072) of SNPs were classified as polymorphic variants in pacu and tambaqui, respectively. Most of the SNPs segregated within each population ranging from 14,199 to 19,856 in pacu; and from 15,075 to 20,380 in tambaqui. Our results indicate high levels of genetic diversity and clustered samples according to their hatchery origin. The developed SerraSNP array represents a valuable genomic tool approaching in-depth genetic studies for these species.

Rayleigh wave group velocity maps at periods of 10–150 s beneath South America

SUMMARY Based on new data from permanent and temporary networks, we present fundamental mode Rayleigh wave group velocity maps at periods of 10–150 s related to the lithosphere beneath South America. We analyse waveform data from 1043 earthquakes, from 2002 to 2019, which were recorded by 282 stations. To isolate fundamental mode Rayleigh waves, a phase-matched filter is applied, and the measurements of group velocity are obtained from multiple filter analysis techniques. Thus, we obtain 17 838 paths, covering most of the South American continent, which reach their maximum at the period of 30 s and decrease for both shorter and longer periods. We calculate average dispersion curves and probability density distribution of all measured curves to check the consistency of our data set. Then, regionalized group velocity maps are obtained by iteratively combining the fast marching method and the subspace inversion method. The resolution of our models is assessed by checkerboard tests, which show that the synthetic group velocities are well recovered, despite some amplitude and smearing effects in some portions of the model, probably owing to regularization and uneven ray path coverage. Compared to previous group velocity studies for South America, our models present better resolution, mainly for shorter periods. Our maps of 10 and 20 s, for example, show an excellent correlation with the sedimentary thickness (CRUST1.0) and topography density (UNB$\_$TopoDens). Regions of exposed basement and high-density are related to fast group velocities, while sedimentary basins and low-densities are observed as areas of slow group velocities. We identify small-scale fast group velocity heterogeneities that may be linked to the Rio Apa and Rio Tebicuary cratons as well as to the geochronological provinces of the Amazonian Craton. The most striking feature of our map at 40 s is a fast group velocity structure with the same NE trend of the Transbrasiliano lineament, a Neoproterozoic megashear fault that crosses a large part of the South American continent. Our long-period maps sample lithospheric depths, revealing that cratonic areas of South America, such as the Amazonian and São Francisco cratons, correlate well with fast group velocities. Another interesting feature is the presence of a strong group velocity gradient between the Paraná and Chaco-Paraná basins, which nearly coincides with the location of the Western Paraná Suture, a continental-scale gravity discontinuity. From our group velocity maps, we estimate 1-D S-wave velocity depth profiles at 10 locations in South America: Chaco-Tarija Basin, Borborema Province (BP), Amazonian Craton, Paraná Basin, Tocantins Province, Acre Basin (AcB), Altiplano-Puna Volcanic Complex, Mantiqueira Province (MP), Parnaíba Basin and São Francisco Craton. Most of our inverted S-wave velocity profiles show good agreement with the SL2013sv model at lithospheric depths, except the BP, AcB and MP profiles. Particularly for the BP, a low shear wave velocity, from about 75 to 150 km depth, is a feature that is not present in the SL2013sv model and was probably resolved in our model because of our denser ray path coverage. This decreased S-wave velocity may be due to a lithospheric thinning beneath the BP, as already pointed out by previous studies.

Filling the gaps: phylogeography of the self-fertilizing Kryptolebias species (Cyprinodontiformes: Rivulidae) along South American mangroves.

Mangrove killifishes of the genus Kryptolebias have been historically classified as rare because of their small size and cryptic nature. Major gaps in distribution knowledge across mangrove areas, particularly in South America, challenge the understanding of the taxonomic status, biogeographical patterns and genetic structuring of the lineages composing the self-fertilizing "Kryptolebias marmoratus species complex." In this study, the authors combined a literature survey, fieldwork and molecular data to fill major gaps of information about the distribution of mangrove killifishes across western Atlantic mangroves. They found that selfing mangrove killifishes are ubiquitously distributed across the Caribbean, Central and South American mangroves and report 14 new locations in South America, extending the range of both the "Central clade" and "Southern clade" lineages which overlap in the Amazon. Although substantial genetic differences were found between clades, the authors also found further genetic structuring within clades, with populations in Central America, north and northeast Brazil generally showing higher levels of genetic diversity compared to the clonal ones in southeast Brazil. The authors discuss the taxonomic status and update the geographical distribution of the Central and Southern clades, as well as potential dispersal routes and biogeographical barriers influencing the distribution of the selfing mangrove killifishes in the western Atlantic mangroves.

Potential displacement of the native Tenuisvalvae notata by the invasive Cryptolaemus montrouzieri in South America suggested by differences in climate suitability.

Tenuisvalvae notata (Mulsant) (Coccinellidae) is a predatory ladybird beetle native to South America. It specializes in mealybugs prey (Pseudococcidae), but relatively little is known about its ecology. In contrast, the ladybird beetle Cryptolaemus montrouzieri Mulsant (Coccinellidae) is indigenous to Australia and has been introduced to many countries worldwide including Brazil for biological control of mealybugs. The potential impacts of these introductions to native coccinellids have rarely been considered. The software CLIMEX estimated the climate suitability for both species as reflected in the Ecoclimatic Index (EI). Much of South America, Africa, and Australia can be considered climatically suitable for both species, but in most cases, the climate is considerably more favorable for C. montrouzieri than T. notata, especially in South America. The CLIMEX model also suggests seasonal differences in growth conditions (e.g. rainfall and temperature) that could affect the phenology of both species. These models suggest that few locations in South America would be expected to provide T. notata climatic refugia from C. montrouzieri. Although other ecological factors will also be important, such as prey availability, this analysis suggests a strong potential for displacement of a native coccinellid throughout most of its range as a consequence of the invasion by an alien competitor.

Postprocessing continental-scale, medium-range ensemble streamflow forecasts in South America using Ensemble Model Output Statistics and Ensemble Copula Coupling

Probabilistic hydrological forecasting and ensemble techniques have leveraged streamflow prediction at regional to continental scales up to several weeks in advance. However, ensembles that only account for meteorological forecast uncertainty are typically biased and subject to dispersion errors, thus limiting their use for rational decision-making and optimization systems. Statistical postprocessing is therefore necessary to convert ensemble forecasts into calibrated and sharp predictive distributions, and it should also account for dependencies between lead times to enable realistic forecast trajectories. This work provides a continental-scale assessment of the use of statistical postprocessing on medium-range, ensemble streamflow forecasts over South America (SA). These forecasts were produced through a large-scale hydrologic–hydrodynamic model forced with a global precipitation dataset and ECMWF reforecast data. The Ensemble Model Output Statistics (EMOS) technique was used to generate conditional predictive distributions in 488 locations at each forecast lead time, while the Ensemble Copula Coupling method with the transformation scheme (ECC-T) was applied to derive ensemble traces from EMOS distributions. Postprocessed streamflow forecasts were cross-validated for the period from 1996 to 2014 using a range of verification metrics. Results showed that the skill and reliability of EMOS forecasts substantially improve over the raw ensembles, and that EMOS leads to skillful predictions relative to discharge climatology and persistence forecasts up to 15 days in advance in most locations. Furthermore, EMOS results in predictive distributions that are generally sharper than the climatology. Limitations in depicting autocorrelations of forecast trajectories were observed in rivers for which the raw ensemble spread is very low and EMOS has to largely increase dispersion, especially at short lead times. The study’s findings suggest that combining a continental-scale hydrological model with EMOS and ECC-T methods can lead to skillful predictions and realistic ensemble traces in several locations in SA, even if in situ hydrometeorological observations are not available in real time.

Habitat loss on the breeding grounds is a major contributor to population declines in a long-distance migratory songbird.

Many migratory species are declining and for most, the proximate causes of their declines remain unknown. For many long-distance Neotropical migratory songbirds, it is assumed that habitat loss on breeding or non-breeding grounds is a primary driver of population declines. We integrated data collected from tracking technology, community science and remote sensing data to quantify migratory connectivity (MC), population trends and habitat loss. We quantified the correlation between forest change throughout the annual cycle and population declines of a long-distance migratory songbird, the Connecticut warbler (Oporornis agilis, observed decline: -8.99% yr-1). MC, the geographic link between populations during two or more phases of the annual cycle, was stronger between breeding and autumn migration routes (MC = 0.24 ± 0.23) than between breeding and non-breeding locations (MC = -0.2 ± 0.14). Different Connecticut warbler populations tended to have population-specific fall migration routes but overlapped almost completely within the northern Gran Chaco ecoregion in South America. Cumulative forest loss within 50 km of breeding locations and the resulting decline in the largest forested patch index was correlated more strongly with population declines than forest loss on migratory stopover regions or on wintering locations in South America, suggesting that habitat loss during the breeding season is a driver of observed population declines for the Connecticut warbler. Land-use practices that retain large, forested patches within landscapes will likely benefit breeding populations of this declining songbird, but further research is needed to help inform land-use practices across the full annual cycle to minimize the impacts to migratory songbirds and abate ongoing population declines.

The genetic characterization of fall armyworm populations in Ecuador and its implications to migration and pest management in the northern regions of South America.

The fall armyworm (Spodoptera frugiperda) is a moth pest native to the Western Hemisphere that has recently become a global problem, invading Africa, Asia, and Australia. The species has a broad host range, long-distance migration capability, and a propensity for the generation of pesticide resistance traits that make it a formidable invasive threat and a difficult pest to control. While fall armyworm migration has been extensively studied in North America, where annual migrations of thousands of kilometers are the norm, migration patterns in South America are less understood. As a first step to address this issue we have been genetically characterizing fall armyworm populations in Ecuador, a country in the northern portion of South America that has not been extensively surveyed for this pest. These studies confirm and extend past findings indicating similarities in the fall armyworm populations from Ecuador, Trinidad-Tobago, Peru, and Bolivia that suggest substantial migratory interactions. Specifically, we found that populations throughout Ecuador are genetically homogeneous, indicating that the Andes mountain range is not a long-term barrier to fall armyworm migration. Quantification of genetic variation in an intron sequence describe patterns of similarity between fall armyworm from different locations in South America with implications for how migration might be occurring. In addition, we unexpectedly found these observations only apply to one subset of fall armyworm (the C-strain), as the other group (R-strain) was not present in Ecuador. The results suggest differences in migration behavior between fall armyworm groups in South America that appear to be related to differences in host plant preferences.

Digitization of the Carnegie Analog Broadband Instruments Tape Records (1965–1996)

AbstractBetween 1965 and 2003, the Carnegie Institution of Washington’s Department of Terrestrial Magnetism operated a continuous network of nine broadband seismographs with locations in South America, Japan, Iceland, Papua New Guinea, and Washington, D.C. The Carnegie seismographs designed in the 1960s by Selwyn Sacks were among the earliest broadband instruments, sensing between at least 30 s and ∼30 Hz. Given the scarcity of historic seismic data of comparable bandwidth and dynamic range prior to the widespread shift to force-feedback instruments and digital recording around the mid-1980s, this dataset is still of high scientific value today.The Carnegie seismographs recorded data to magnetic tapes meant to be read and analyzed using a custom playback system. Since 1989 these tapes have been stored in a climate-controlled, electromagnetically shielded room, which preserved them in reasonably good condition. However, some tapes now show signs of moisture damage, and reading them is difficult and time consuming by today’s standards, creating a barrier to the use of this dataset. To overcome these issues, we have undertaken an ongoing effort to digitize this dataset with the goal of making it publicly available in Standard for the Exchange of Earthquake Data (SEED) format at the Incorporated Research Institutions for Seismology Data Management Center (IRIS DMC).

Geographical Potential of Solar Thermochemical Jet Fuel Production

The solar thermochemical fuel pathway offers the possibility to defossilize the transportation sector by producing renewable fuels that emit significantly less greenhouse gases than conventional fuels over the whole life cycle. Especially for the aviation sector, the availability of renewable liquid hydrocarbon fuels enables climate impact goals to be reached. In this paper, both the geographical potential and life-cycle fuel production costs are analyzed. The assessment of the geographical potential of solar thermochemical fuels excludes areas based on sustainability criteria such as competing land use, protected areas, slope, or shifting sands. On the remaining suitable areas, the production potential surpasses the current global jet fuel demand by a factor of more than fifty, enabling all but one country to cover its own demand. In many cases, a single country can even supply the world demand for jet fuel. A dedicated economic model expresses the life-cycle fuel production costs as a function of the location, taking into account local financial conditions by estimating the national costs of capital. It is found that the lowest production costs are to be expected in Israel, Chile, Spain, and the USA, through a combination of high solar irradiation and low-level capital costs. The thermochemical energy conversion efficiency also has a strong influence on the costs, scaling the size of the solar concentrator. Increasing the efficiency from 15% to 25%, the production costs are reduced by about 20%. In the baseline case, the global jet fuel demand could be covered at costs between 1.58 and 1.83 €/L with production locations in South America, the United States, and the Mediterranean region. The flat progression of the cost-supply curves indicates that production costs remain relatively constant even at very high production volumes.

What Can Integrated Analysis of Morphological and Genetic Data Still Reveal about the Anastrepha fraterculus (Diptera: Tephritidae) Cryptic Species Complex?

The South American fruit fly Anastrepha fraterculus (Wiedemann) is a complex of cryptic species, the so-called “Anastrepha fraterculus complex”, for which eight morphotypes are currently recognized. A previous analysis of ITS1 in samples of the Anastrepha fraterculus complex, while revealing high distinctiveness among samples from different localities of South America, Central America, and Mexico, no direct association was made between sequence type and morphotype. In the present report, a correlated analysis of morphometry and ITS1 data involved individuals belonging to the same population samples. Although showing a low level of intra-populational nucleotide variability, the ITS1 analysis indicated numerous inter-population sequence type variants. Morphotypes identified by morphometric analysis based on female wing shape were highly concordant with ITS1 genetic data. The correlation of genetic divergence and morphological differences among the tested samples gives strong evidence of a robust dataset, thereby indicating the existence of various taxonomic species within the A. fraterculus complex. However, the data revealed genetic and morphological variations in some regions, suggesting that further analysis is still required for some geographic regions.

A revised and geo-referenced list of type localities for termites in South America.

A revised, geo-referenced, and cross-referenced list of 270 termite type localities in South America is presented. A total of 543 nominal species-group extant taxa are listed, 442 of them valid and 97 synonyms. The type-locality comprising the greatest number of nominal taxa in South America is Kartabo, Guyana (A.E. Emerson study site), with 59 taxa, followed by Cuiabá, Brazil (F. Sivestri), with 34, and Ribeirão Cascalheira, Brazil (Xavantina-Cachimbo Expedition, A.G.A. Mathews) with 26.

Taxonomic revision of the New World big-eyed minute litter bug genusOmmatides Uhler (Hemiptera: Schizopteridae).

Hypselosomatinae, the big-eyed minute litter bugs, are diagnosed among Schizopteridae (infraorder Dipsocoromorpha) by the large eye, 4-segmented labium, and distinct wing venation. The group was recovered as sister taxon to all remaining Schizopteridae in recent molecular phylogenetic analyses. Described species diversity of Hypselosomatinae (13 extant genera prior to this study) is greatest in the Old World and in particular the Australian region, while only five monotypic or small genera are currently described from the New World (Glyptocombus Heidemann, Ommatides Uhler, Williamsocoris Carpintero Dellapé, Hypsohapsis Hoey-Chamberlain Weirauch, and Hypselosomops Hoey-Chamberlain Weirauch). Based on examination of 60 specimens of Hypselosomatinae from South America, we here synonymize Ommatides that includes one described species from the Lesser Antilles with the monotypic genus Williamsocoris Carpintero Dellapé from Argentina, describe seven new species of Ommatides (O. duodentis sp. nov., O. nudus sp. nov., O. parvidentis sp. nov., O. pillcopata sp. nov., O. pristis sp. nov., O. tridentis sp. nov., O. yoderi sp. nov. and O. zanderij sp. nov.) from various locations in South America, and redescribe Ommatides. We provide thorough documentation of morphological features using macroimages, SEM, and line drawings for new taxa and Ommatides insignis Uhler and a distribution map for all currently known New World Hypselosomatinae.

South American characids share very similar ionoregulatory characteristics

We examined ionoregulatory characteristics of four Characids from diverse locations in South America, emperor tetras (Nematobrycon palmeri), penguin tetras (Thayeria boehlkei), serpae tetras (Hyphessobrycon eques), and rosy tetras (Hyphessobrycon rosaceous). When held in 100 μmol L−1 Na+ water, tetras had Jmax values over 1100 nmol g−1 h−1, and Km values below 60 μmol L−1. When held in 1 mmol L−1 Na+ water kinetic parameters were unchanged. Low pH had no effect on Na+ uptake (JinNa). At pH 3.25, Na+ loss (JoutNa) was stimulated 35–85% in two of the four species. To test the linkage of JinNa to NH3 and H+ extrusion we measured JinNa during exposure to 1 mmol L−1 NH4Cl (HEA) and 100 μmol L−1 Acetazolemide (AZ). HEA stimulated JinNa of emperor tetras by 40%, but inhibited JinNa of penguin tetras by 50%; the two remaining species were unaffected. AZ (an inhibitor of carbonic anhydrase) inhibited JinNa of serpae tetras by 40%, but had no effect on the others. All tetras displayed ionoregulatory characteristics that are very similar to each other, which supports the argument that these physiological traits may be ancestral for this group and pre-date colonization of the Rio Negro. The novel finding that, Jmax and Km did not change after acclimation to 1 mM Na+ water indicates that, unlike in other species examined uptake is not plastic. The HEA and AZ results, along with pH insensitivity suggest Na+ uptake is not coupled to H+ extrusion or NH3 excretion and leaves the exact mechanism involved unclear.

Morphological and molecular characterization of an enigmatic clinostomid trematode (Digenea: Clinostomidae) parasitic as metacercariae in the body cavity of freshwater fishes (Cichlidae) across Middle America.

The family Clinostomidae Lühe, 1901 contains 29 species allocated to seven genera, of which Clinostomum Leidy, 1856 is the most diverse, with c. 14 valid species. The diversity of Clinostomum has been assessed, combining morphological and molecular data. The genetic library for species in this genus has increased steadily, although there is little or no information for the other genera included in the family. Molecular phylogenetic relationships among the genera of clinostomids have not been assessed, and their classification is still based on morphological traits. The monotypic Ithyoclinostomum was described from a fish-eating bird in Brazil, and its metacercariae have been found in several locations in South America, parasitizing erythrinid freshwater fishes. We collected unusually large metacercariae from the body cavity of cichlids in several locations across Middle America. These metacercariae exhibited some resemblance to Ithyoclinostomum, although several differences prevent their inclusion in Ithyoclinostomum dimorphum, casting doubt on their taxonomic identification. The main objective of this paper was to characterize the metacercariae collected in cichlids using both morphology and molecular data from three molecular markers, and to assess the molecular phylogenetic relationships among the genera of Clinostomidae to establish the position of the newly generated sequences. We took a conservative position and tentatively placed the metacercariae as belonging to Ithyoclinostomum.

Morphological and molecular characterization of Diaporthe (anamorph Phomopsis) complex and pathogenicity of Diaporthe aspalathi isolates causing stem canker in soybean

The complex of Diaporthe (asexual morph) species occurring on soybean constitutes an important pathogenic group associated with diseases such as pod and stem blight, seed decay and stem canker. Stem canker, caused by Diaporthe aspalathi, has been reported as the most aggressive form of canker and its occurrence has limited soybean crop productivity in the southern United States. The main form of pathogen control is the use of stem canker resistant soybean varieties. In this study, strains of Diaporthe and Phomopsis were isolated from stem and seeds of soybean in different locations in South America during the years 1989–2014. Genomic DNA from 26 isolates were analyzed by PCR-restriction fragment length polymorphism (RFLP) and Amplified Fragment Length Polymorphism (AFLP) techniques, and sequencing of internal transcribed spacer (ITS) regions of ribosomal DNA. The molecular analysis of ITS sequences by alignment with those of ex-type strains deposited in GenBank and morphological characteristics allowed the identification of Phomopsis longicolla, D. phaseolorum var. sojae, D. caulivora and D. aspalathi. An analysis of the pathogenicity of 13 isolates of D. aspalathi inoculated in soybean genotypes carrying different resistance genes to stem canker (Rdm1, Rdm2, Rdm3, Rdm4, Rdm5 and Rdm?) enabled us to identify the occurrence of at least three races of D. aspalathi occurring in Brazil. Among the isolates identified as D. aspalathi, both molecular and phenotypic analyses showed clustering depending on the date of collection and pathogenicity, which revealed the existence of variability of the pathogen.

Evolutionary structure of Plasmodium falciparum major variant surface antigen genes in South America: Implications for epidemic transmission and surveillance.

Strong founder effects resulting from human migration out of Africa have led to geographic variation in single nucleotide polymorphisms (SNPs) and microsatellites (MS) of the malaria parasite, Plasmodium falciparum. This is particularly striking in South America where two major founder populations of P. falciparum have been identified that are presumed to have arisen from the transatlantic slave trade. Given the importance of the major variant surface antigen of the blood stages of P. falciparum as both a virulence factor and target of immunity, we decided to investigate the population genetics of the genes encoding “Plasmodium falciparum Erythrocyte Membrane Protein 1” (Pf EMP1) among several countries in South America, in order to evaluate the transmission patterns of malaria in this continent. Deep sequencing of the DBLα domain of var genes from 128 P. falciparum isolates from five locations in South America was completed using a 454 high throughput sequencing protocol. Striking geographic variation in var DBLα sequences, similar to that seen for SNPs and MS markers, was observed. Colombia and French Guiana had distinct var DBLα sequences, whereas Peru and Venezuela showed an admixture. The importance of such geographic variation to herd immunity and malaria vaccination is discussed.