Localization Of Neuron-specific Enolase Research Articles

Immunohistochemical localization of neuron specific enolase in developing tongue of Korean native goat (Capra hircus)

Neuron-specific enolase (NSE), a brain specific isoenzyme of the glycolytic enzyme, is characterized by its consistent occurrence in the cytoplasm of mature neurons. The purpose of the present study was to investigate the expression of NSE in the developing tongue of Korean native goats. The tongues of the fetuses were removed from 2- to 4-year-old female Korean native goats by caesarean section performed under general anesthesia. The expression of NSE in the developing tongue of goat fetuses (60, 90, 120, and 150 days) was studied using immunohistochemistry. In 60-day-old fetuses, NSE-immunoreactivity (IR) exhibited weak appearance in lamina propria of the basal portion and the apical epithelia of the tongue. In 90-day-old fetuses, NSE-nerve fibers were extended in the core part of connective tissue, and primordia of the taste buds was moderately positive. In 120-day-old fetuses, NSE-IR was strongly expressed in taste buds and gustatory nerve fibers. In neonates, the taste buds of vallate papillae were strongly positive for NSE, and development of nerve fibers was synapsed with connective tissue of well innervated taste buds. These results indicate that NSE expressions were associated with the sign of nerve innervation in prenatal development of goat tongues and NSE may be a useful neuronal marker to understand the development of gustatory nerve innervations.

Neuron-specific enolase in the intestinal wall in Crohn´s disease.

The authors described the localization of neuron-specific enolase in the intestinal wall in Crohn´s disease. We have used samples obtained by biopsy from the colon lining of five people affected by Crohn's disease for our examination. We have processed samples using the formol paraffin technique. From paraffin blocks, we have prepared histological sections approximately 5 μm thick. For immunohistochemic examinations, we have revitalised the sections by acquiring the heat-induced epitope. We detected NSE by monoclonal mouse antibodies against human neuron-specific enolase, clone BBS/NC/VI-H14 (DakoCytomation, Denmark) (Fig. 4, Ref. 7).

Immunohistochemical Localization of Neuron Specific Enolase and CD3 Lymphocyte Activation

Immunohistochemical localization of CD3 and Neuron Specific Enolase (NSE) to demonstrate neuronal metabolism in proliferation and cell recovery was carried out in this study to evaluate morphological and cellular changes in cyanide treated perfused cortical tissues of adult wistar rats in vitro. Four separately perfused tissues were stained with antibodies specific for neuron specific enolase using antigen retrieval method and colour reaction involving 3’3’-Di aminobenzidine tetrachloride (DAB) in peroxidase anti peroxidase method. Treated cells showed increased in enolase activity implying increase proliferation activity for repair purposes in the assaulted neuron (cyanide treated neurons). Presence of CD3 activity also indicates the presence of undifferentiated T-lymphocytes around the cells undergoing apoptosis in the cortex following treatment with cyanide

Aminergic innervation of the gall bladder in man and dog

This study has compared the innervations of human and canine gall bladders, using immunohistochemical localization of neuron-specific enolase to visualize all intramural nerves and localization of tyrosine hydroxylase, DOPA decarboxylase and serotonin to visualize different populations of aminergic neurons. The vasculature, the wall smooth musculature and the intramural ganglion cells receive a substantial sympathetic innervation, in both species. By contrast, the mucosa appears to be supplied almost entirely by non-sympathetic fibres. On the basis of DOPA decarboxylase immunoreactivity, a large proportion of the biliary sympathetic nerves in both species may be dopaminergic. Human gall bladder also contains a population of putatively tryptaminergic, intramural neuron-like cells. However, there was no evidence for tryptaminergic innervation of effector structures in the gall bladder wall.

Neuron-specific enolase and its mRNA are highly expressed in large congenital nevi: a study using immunocytochemistry, biochemical assay, and in situ hybridization.

The malignant transformation of congenital nevocellular nevi, both large and small, is controversial and presents problems in management. The size of the lesion is taken to indicate potential malignant transformation, but this is an arbitrary scale. A more reliable biological indicator is needed to help predict the lesions at risk. Following the localization of neuron-specific enolase to most cells of the diffuse neuroendocrine system and their neoplasms (including benign and malignant melanocytic lesions), it has been suggested that its level is related to tumor activity. In a prospective trial, the presence of neuron-specific enolase immunoreactivity, its concentration, and gene expression in nevus cells were studied in 31 congenital melanocytic nevi of various sizes (1.5 cm to bathing trunk) using immunocytochemistry, biochemical assay, and in situ hybridization. Twenty-five of the 31 congenital nevi were immunoreeactive to neuron-specific enolase antiserum, with stronger immunostaining in the larger lesions. There is an apparent linear relationship between the size of the nevi and the level of neuron-specific enolase (expressed as nanograms per milligram protein). Neuron-specific enolase mRNA was highly expressed in most of the large congenital nevi (greater than 15 cm in diameter), as revealed by autoradiography following in situ hybridization. Our results show that neuron-specific enolase and its mRNA are expressed to a greater extent in large congenital nevi compared with the smaller lesions. This might prove to be a useful indicator of those lesions at risk of malignant transformation.

Serum neuron-specific enolase. A marker for response to therapy in seminoma

Neuron-specific enolase (NSE) was evaluated as a serum marker in 105 patients with testicular cancer and compared with the established tumor markers alphafetoprotein (AFP) and human chorionic gonadotropin (HCG). Increased serum NSE activity was measured in eight of 11 (73%) patients with metastatic seminoma. Serum NSE concentrations fell to within the normal range following chemotherapy. Localization of NSE in seminoma cells was demonstrated immunohistochemically. Only six of 40 (15%) patients with metastatic nonseminomatous germ cell tumors showed elevated serum NSE levels. AFP and HCG were both positive in 70% of patients in this group, and NSE determination gave no additional information. Serum NSE concentrations were normal in 53 of 54 testicular cancer patients after orchiectomy and there was no evidence of metastatic disease; only one had borderline NSE levels, indicating the specificity of serum NSE determination. NSE is a new marker of seminoma and its measurement may be of clinical value in monitoring chemotherapy in patients with metastatic seminoma.

Localization of neuron-specific enolase (NSE) mRNA in human brain

In situ hybridization with biotinylated DNA and RNA probes derived from the 3′ untranslated region of human neuron-specific or γγ-enolase (NSE) permits histochemical detection of NSE mRNA in autopsy samples of human brain. In both aged controls and Alzheimer's disease (AD) patients, NSE probe hybridized with variable intensity to nerve cells in visual cortex and cerebellum, but not to glial or liver cells. These results provide evidence that (1) NSE mRNA is expressed in neurons in human brain tissue, (2) pattern of NSE mRNA expression in unaffected brain regions in AD is qualitatively normal, and (3) NSE mRNA expression may vary widely among different classes of neurons.

Immunocytochemical evidence of neuron specific enolase (NSE) in human adenovirus 12-induced retinoblastoma-like tumor cells in vitro.

Retinoblastoma-like tumor cells (EXP-5 cell line) derived from a tumor in an inbred albino rat by human adenovirus 12 have been maintained in culture. We investigated immunocytochemical localization of neuron specific enolase (NSE) and S-100 protein in cultured tumor cells before and after successful differentiation into neuronal and glial-like cells. After the addition of cyclic AMP analog, a neurite-like process developed, and they tended to form a network after 24hr. Before and after successful differentiation, the cultured cells exhibited simultaneous expression of NSE and S-100 protein. Differentiated cells showed a tendency to express the marker that correlated with their morphology. These results supported the idea that adenovirus 12-induced retinoblastoma-like tumor was derived from bipotential precurcer cells.

Ultrastructural immunocytochemical localization of neuron-specific enolase in parafollicular cells of the rat thyroid.

Using pre- and post-embedding procedures, neuron-specific enolase and calcitonin were localized in rat thyroid parafollicular cells by light and electron microscopy. Peroxidase-antiperoxidase (PAP), biotin-avidin (ABC) and protein A--colloidal gold techniques were used. In paraffin sections neuron-specific enolase was demonstrated in all calcitonin-storing parafollicular cells in rats aging 1 to 180 days. The post-embedding procedure failed to detect neuron-specific enolase in ultrathin sections, but the enzyme could be demonstrated using a preembedding procedure. Neuron-specific enolase was localized exclusively within the cytosol of parafollicular cells, while calcitonin was localized within secretory granules applying either post- or pre-embedding incubation techniques.

Immunocytochemical study on the localization of neuron-specific enolase and S-100 protein in the carotid body of rats

Light- and electron-immunocytochemical investigation with the peroxidase-antiperoxidase (PAP) procedure revealed neuron-specific enolase and S-100 protein-like immunoreactivities specifically localized in the chief cells and the sustentacular cells of the rat carotid body, respectively. This finding suggests a neuron-like nature of the chief cells and a glia-like nature of the sustentacular cells on both embryological and functional bases.

Localization of neuron-specific enolase in mouse spinal neurons grown in tissue culture

Neuron-specific enolase (NSE) is an isoenzyme of the glycolytic enzyme enolase (EC 4.2.1.11) which is also has a muscle and liver isoenzyme. Previous work has shown NSE to be specifically localized to neurons and neuroendocrine cells, but the application of NSE as a marker for cell cultures has not been investigated. Primary culture of central nervous system tissue derived from mice have been used to study optimal fixation procedures. The results show that NSE can serve as a useful alternative to non-specific histochemical stains or strictly morphologic criteria for identifying nerve cells.