Location Of Inflammation Research Articles

Microwave-assisted synthesis and 99mTc-radiolabeling of anti-inflammatory active curcumin derivatives for inflammation diagnosis and therapy

AbstractUsing a one-pot cyclo-condensation process under microwave irradiation, derivatives of curcumin have been created. Evaluation their anti-inflammatory efficacy showed that they had stronger properties than curcumin. Additionally, the most active derivative was radiolabeled with the diagnostic radioisotope technetium-99m and prepared with a high radiochemical yield (96.5 ± 0.09%) and in vitro stability of up to 6 h. The in-vivo study in inflamed mice showed that [99mTc]Tc-curcumin derivative accumulated with a high target to non-target ratio. The gathered information confirmed the efficacy of the [99mTc]Tc-curcumin derivative as a novel possible tracer for the identification and localization of inflammation.

Surgical Management of Crohn’s Disease: An Overview

Crohn's Disease (CD) is a chronic inflammatory condition of the digestive tract resulting in symptoms such as abdominal pain, weight loss, and fatigue, requiring a multifaceted approach to management. While not curative, the surgical management of CD forms a vital part of the therapeutic strategy for complications. This article overviews various surgical methods for treating CD, their indications, and potential complications. CD predominantly emerges in developed countries, with known risk factors including genetic predisposition, environmental triggers, and diet. The clinical presentation involves varied symptoms based on the inflammation's location within the gastrointestinal tract. Diagnosis necessitates a comprehensive approach, including medical history, physical examination, and diagnostic tests. Surgical intervention may be necessary due to severe complications like intestinal obstruction, abscesses, strictures, fistulas, and perforation. Common reasons for surgery include medically resistant disease and malignancy. However, postoperative recurrence is frequent, necessitating repeated surgery in 26% of cases. This narrative review paper examines three major surgical interventions in detail. Fistulotomy, primarily used for simple fistulas, can offer symptomatic relief in well-selected CD cases but may risk complications and high recurrence rates. Stricturoplasty, useful for widening narrow bowels, preserves intestinal length and function and can serve as an effective alternative to resection. Lastly, the intestinal resection and colectomy procedures are explored, focusing on their indications and complications. Future research should focus on optimizing surgical approaches to reduce recurrence rates and improve patient outcomes.

Diagnosis and treatment of autoimmune diseases of the brain and spinal cord in dogs: analysis of the literature review

The main methods of diagnosing these diseases, according to the analysis of world literature data, are the analysis of the patient's cerebrospinal fluid and magnetic resonance imaging (MRI). The analysis of cerebrospinal fluid (CSF) is carried out in order to detect changes characteristic of the diagnosis. With the help of MRI, it is possible to determine the localization of inflammation. A number of studies have been aimed at improving diagnostic methods by detecting new factors characteristic of autoimmune diseases of the nervous system. Data on the detection of antibodies corresponding to human antibodies in the cerebrospinal fluid of dogs and on changes in the concentration of various substances in it are presented. This allows for faster and more accurate diagnostics.As a result of the generalization of the literature data, it was found that immunosuppressive drugs are used for the treatment of autoimmune diseases of the brain and spinal cord of dogs. According to the data presented, the most effective of the known methods of treatment of autoimmune meningoencephalitis is the use of a combination of prednisolone and imatinib mesylate. For the treatment of steroid-dependent meningoarteritis (SRMA), various corticosteroid drugs are used, the most optimal of which is the combination of prednisone and cytarabine.The purpose of our study was to study modern Russian and foreign literature sources on autoimmune diseases of the brain and spinal cord in dogs, methods of diagnosis and treatment of these diseases and to review works that provide new data on their treatment, diagnosis and understanding of etiology and pathogenesis.

Stromal inflammatory subtypes of oral squamous cell carcinoma correlate with patient clinical characteristics, demographics and gene expression

<h3>Introduction</h3> Oral squamous cell carcinoma (OSCC) is the most common oral malignancy displaying significant mortality and morbidity. The current OSCC grading systems exclusively describe the tumor differentiation, and share limited morphologic characterization of the stroma. Herein, we present a comprehensive microscopic analysis of the OSCC stromal inflammation and its relation with clinical features, demographics and gene expression of these patients. <b>Materials and Methods:</b> 87 completely excised OSCC tissues were prospectively collected. OSCC microenvironment was characterized according to the immune phenotype, the degree of desmoplasia and the extent and localization of inflammation. Statistical correlations between the tumor stroma and the patients' characteristics were carried out using GraphPad Prism. KEGG pathway analysis of representative PanCancer IO 360^TMdata was also investigated using nSolver software. <h3>Results</h3> Inflamed tumors were more commonly encountered (49 cases) compared with non-inflamed OSCC (31 immune-excluded and 7 immune-deserted). Peritumoral fibrosis (36 tumors) correlated with male gender (p=0.0043), smoking (p=0.0455), alcohol consumption (p=0.0044), increased tumor size (p=0.0054) and stage (p=0.002) while a peritumoral inflammation (60 cases) was mainly seen in females (p=0.0105), non-drinkers (p=0.0329), and was associated with decreased size (p=0.0044). No association between stromal characteristics and tumor grade was seen. Pathway analysis showed differential gene expression between triplicates of inflamed and immune-excluded tumors in cancer pathways and chemokine or cytokine signaling. <h3>Conclusions</h3> Our work describes the relation of carcinogens (alcohol and tobacco) with distinct stromal phenotypes in oral cancer. Additionally, the correlation with tumor size and stage complements previous work indicating a worst biologic behavior in "cold" tumors. In addition, the underlying molecular pathways that can be implicated in different stromal phenotypes are highlighted. Collectively, these findings in addition to the indications that the microenvironment shares microscopic features independent of the tumor grade highlight the necessity of their incorporation into future grading systems of OSCC.

Selective potentiometric detection of reactive oxygen species (ROS) in biologically relevant concentrations by a modified metalized polyporphyrine sensing layer coated with nonbiofouling poly(2-alkyl-2oxazoline)s

We built a robust selective potentiometric sensor of reactive oxygen species (ROS) as a thin layer applied on the surface of smart implants, which enables the detection and localization of inflammation at an early stage. The sensor consists of a conductive polymer layer based on polythiophene with an incorporated porphyrin-metal complex that potentiometrically detects the presence of ROS. This sensor is covalently coated with a nonbiofouling layer of poly(2-methyl-2-oxazoline), which works as a biocompatibilizer but mainly prevents the sorption of proteins and other biomacromolecules naturally occurring in organisms, which could interfere with the ROS signal. We have shown that our potentiometric sensor shows a rapid response to hydrogen peroxide, does not experience interference with bovine serum albumin as a model serum protein when sensing ROS, is able to fully reversibly detect ROS with a linear response within a very wide range of biologically relevant concentrations and, most importantly, is able to distinguish between hydrogen peroxide and hypochlorite. We also performed a head-to-head comparison of two positional isomers of thienylated porphyrine for sensor applications (2TTP and 3TTP, with 3TTP shown to be more appropriate) and four different coordinated metals (Cu, Fe, Co and Mn, with Cu and especially Fe shown to be the most appropriate).

Preparation and preliminary evaluation study of [131I]iodocolchicine-gallic-AuNPs: a potential scintigraphic agent for inflammation detection

Background Nanomedicine offers great potential for scintigraphic diagnostic imaging with lower risk and higher quality compared to other traditional techniques. Objectives This work aimed to develop and evaluate gold nanoparticles combined with gallic acid (gallic-AuNPs) and [131I]iodocolchicine as a scintigraphic probe for inflammation. Methods [131I]iodocolchicine-gallic-AuNPs were synthesized via chemical reduction method where gallic acid was used as reducing agent and [131I]iodocolchicine was used as stabilizing agent. Then a characteristic profile for the synthesized nano-platform was performed including size analysis, zeta potential, radiochemical yield and in-vivo biodistribution in inflammation bearing mice. Results and Conclusion This platform was successfully synthesized with good stability, appropriate particle size (10 nm diameter for AuNPs), and high radiochemical purity for [131I]iodocolchicine (96.79%). The in-vivo study indicated that [131I]iodocolchicine-gallic-AuNPs accumulated with a high target to non-target ratio in intravenous injection and high retention value in intra-inflammation injection in inflammation model. The obtained data supported the usefulness of the new platform ([131I]iodocolchicine-gallic-AuNPs) as a tracer for the detection and localization of inflammation.

An IFN-STAT Axis Augments Tissue Damage and Inflammation in a Mouse Model of Crohn's Disease.

Blocking interferon-function by therapeutic intervention of the JAK-STAT-axis is a novel promising treatment option for inflammatory bowel disease (IBD). Although JAK inhibitors have proven efficacy in patients with active ulcerative colitis (UC), they failed to induce clinical remission in patients with Crohn's disease (CD). This finding strongly implicates a differential contribution of JAK signaling in both entities. Here, we dissected the contribution of different STAT members downstream of JAK to inflammation and barrier dysfunction in a mouse model of Crohn's disease like ileitis and colitis (Casp8ΔIEC mice). Deletion of STAT1 in Casp8ΔIEC mice was associated with reduced cell death and a partial rescue of Paneth cell function in the small intestine. Likewise, organoids derived from the small intestine of these mice were less sensitive to cell death triggered by IBD-key cytokines such as TNFα or IFNs. Further functional in vitro and in vivo analyses revealed the impairment of MLKL-mediated necrosis as a result of deficient STAT1 function, which was in turn associated with improved cell survival. However, a decrease in inflammatory cell death was still associated with mild inflammation in the small intestine. The impact of STAT1 signaling on gastrointestinal inflammation dependent on the localization of inflammation, as STAT1 is essential for intestinal epithelial cell death regulation in the small intestine, whereas it is not the key factor for intestinal epithelial cell death in the context of colitis. Of note, additional deletion of STAT2 was not sufficient to restore Paneth cell function but strongly ameliorated ileitis. In summary, we provide here compelling molecular evidence that STAT1 and STAT2, both contribute to intestinal homeostasis, but have non-redundant functions. Our results further demonstrate that STATs individually affect the distinct pathophysiology of inflammation in the ileum and colon, respectively, which might explain the diverse outcome of JAK inhibitors on inflammatory bowel diseases.

Application of high-resolution optical coherence tomography for visualization of eye structures in uveitis of different localization

The article presents an analysis of publications in main Russian and foreign journals devoted to the use of optical coherence tomography in intravital visualization of the structures of the eye for diagnosis, assessment of the changes during relapses, recovery and remission stages, as well as the effectiveness of treatment for uveitis of different localization. It describes in detail the results of studies conducted using spectral optical coherence tomography, changes in the anterior chamber and cornea in uveitis of the anterior localization, morphological changes in the vitreous body, vitreomacular interface, retina, choroid and optic disc in the anterior and posterior localization of inflammation, and presents the features of OCT diagnosis in a number of nosological forms of uveitis based on the literature data.

Fakty i mity na temat antybiotykoterapii

Resistance to antibiotics is becoming a serious clinical problem. It increases the cost of treatment and is the reason for the failure of therapy. It cannot be eliminated, but the principles of prevention must be respected. First, do not prescribe chemotherapeutics in the therapy of viral infections and symptomatic treatment, after the second care to the selection of a sufficiently high dose, recommend regular intake of the drug and do not shorten the time of therapy. It is also important to choose the right active substance, which should be conditioned by the expected sensitivity of bacteria, the localization of inflammation and the age of the patient. Do not forget about the accompanying therapy, including the ordination of the probiotic and antifungal protection.

Differences in autoimmune hepatitis based on inflammation localization.

Histopathology is essential for the diagnosis and evaluation of disease activity of autoimmune hepatitis (AIH). We aimed to elucidate the characteristics of AIH from the localization of inflammation. We re-evaluated a nationwide survey that was performed in Japan in 2018 of AIH patients diagnosed between 2014 and 2017. A total of 303 patients were enrolled, and the clinical and treatment characteristics were compared between the patients with predominantly portal inflammation (230 patients) or lobular inflammation (73 patients). AIH patients with lobular inflammation had a higher probability of being diagnosed with acute hepatitis than those with portal inflammation. Liver enzyme levels were higher in patients with lobular inflammation, whereas immunoglobulin G levels were higher in patients with portal inflammation. The prevalence of an alanine aminotransferase level < 30 U/L after 6months of treatment was significantly higher in patients with lobular inflammation than in those with portal inflammation (81.7% vs. 67.3%, P = 0.046). The localization of inflammation may be useful for evaluating the onset of AIH.

Spondyloarthritis: aspects of pathogenesis and diagnostics

Spondyloarthritis (SpA) has extremely high social importance due to patient’s risk of early disability and a persistent decline in the quality of life. The last decade has brought important knowledge on its genetic mechanisms and pathophysiological aspects, as well as diagnosis and treatment. Axial spondyloarthritis (axSpA) is characterized by a pathological tissue response to immune and mechanical triggers, predominant lesion of the axial skeleton (spine, sacroiliac joints). It is divided into ankylosing spondylitis (AS) and non-radiological axSpA. Mechanical stress is proven to affect specific localization of inflammation and tissue damage in SpA. Areas of inflammation and erosion are limited to those having increased susceptibility and unique microanatomy. It is shown that a number of genes located outside the main histocompatibility complex significantly affect susceptibility to AS. A study of genes and polymorphisms not related to HLA-B27 revealed different pathogenesis mechanisms in various ethnic groups and formed a new understanding of AS pathogenesis and treatment. A family history of AS or anterior uveitis is useful to detect axSpA, as it is associated with the carrier of genetic marker HLA-B27. Researchers are strongly focused on studying AS patients» autoantibodies, where antibodies to CD74 are of the greatest diagnostic value. An imbalanced microbiome can trigger SpA development. Approximately 50 % of all SpA patients have microscopic signs of intestinal inflammation. The development and use of highly sensitive imaging methods in the future will lead to improved approaches for axSpA classification and more precise disease prognosis and therapeutic decisions. 18-fluorodeoxyglucose positron emission tomography is a sensitive method to determine syndesmophytes and sacroiliac joints in axSpA patients.

Spleen stiffness to liver stiffness ratio significantly differs between ALD and HCV and predicts disease-specific complications.

Background & AimsBoth liver stiffness (LS) and spleen stiffness (SS) are widely used to non-invasively assess liver fibrosis and portal hypertension, respectively. We aimed to identify the impact of disease etiology, namely the localization of inflammation (portal vs. lobular), on the SS/LS ratio.MethodsIn this multicenter study, LS and SS were prospectively assessed in 411 patients with alcohol-related liver disease (ALD) or hepatitis C virus (HCV) using FibroScan® (Echosens, Paris); changes in these parameters were also studied in response to treatment (alcohol withdrawal, HCV therapy). LS and spleen length (SL) were further analyzed in a retrospective cohort of 449 patients with long-term data on decompensation/death.ResultsBoth, SS and SL were significantly higher in HCV compared to ALD (42.0 vs. 32.6 kPa, p≪0.0001, 15.6 vs. 11.9 cm, p≪0.0001) despite a lower mean LS in HCV. Consequently, the SS to LS ratio and the SL to LS ratio were significantly higher in HCV (3.8 vs. 1.72 and 1.46 vs. 0.86, p≪0.0001) through all fibrosis stages. Notably, SL linearly increased with SS and the relation between SS and SL was identical in HCV and ALD. In contrast, livers were much larger in ALD at comparable LS. After treatment, LS significantly decreased in both diseases without significant changes to the SS/LS ratio. In the prognostic cohort, patients with ALD had higher LS values (30.5 vs. 21.3 kPa) and predominantly presented with jaundice (65.2%); liver failure was the major cause of death (p≪0.01). In contrast, in HCV, spleens were larger (17.6 vs. 12.1 cm) while variceal bleeding was the major cause of decompensation (73.2%) and death (p≪0.001).ConclusionBoth SS/LS and SL/LS ratios are significantly higher in patients with portal HCV compared to lobular ALD. Thus, combined LS and SS or SL measurements provide additional information about disease etiology and disease-specific complications.Lay summaryHerein, we show that patients with hepatitis C virus infection (HCV) have higher spleen stiffness and portal pressure than patients with alcohol-related liver disease (ALD), within the same fibrosis stage and matched to liver stiffness. Thus, the spleen stiffness to liver stiffness ratio is significantly higher in patients with HCV compared to ALD. Additionally, patients with HCV more commonly progress to portal hypertension-related complications (e.g. variceal bleeding), while patients with ALD more commonly progress to liver failure (e.g. jaundice). The spleen stiffness to liver stiffness ratio is a useful tool to confirm disease etiology and predict disease-specific complications.

Elevation of serum pyruvate kinase M2 (PKM2) in IBD and its relationship to IBD indices

ObjectivesEndoscopy remains the gold standard to diagnose and evaluate inflammatory bowel disease (IBD) activity. Current biomarkers or their combinations cannot adequately predict IBD risk, diagnosis, progression or relapse, and response to therapy. Pyruvate kinase M2 (PKM2) is emerging as a significant mediator of the inflammatory process. We aimed to assess levels of serum PKM2 in healthy and newly diagnosed IBD patients and its relationship with IBD indices and microbiota changes. Design and methodsIBD serum samples from newly diagnosed patients were collected and analyzed using a PKM2-ELISA and correlated with disease activity scores, IBD disease type, and intestinal microbiota. Furthermore, we tested the genetic and protein expression of PKM2 in an in vitro intestinal cell model of inflammation. ResultsSerum PKM2 levels were 6-fold higher in IBD patients compared to healthy controls, with no sensitivity to disease phenotype (Crohn's Disease or Ulcerative Colitis) or localization of inflammation. Serum PKM2 had considerably less interindividual variability than established IBD fecal biomarkers. A positive Pearson correlation (r=0.6121) existed between serum PKM2 and Bacteroidetes fecal levels in Crohn's disease (CD), while a negative (r=−0.6128) correlation was observed with Actinobacteria fecal levels. Furthermore, LPS (500ng/mL) significantly increased PKM2 expression in vitro, which was significantly suppressed by an anti-inflammatory flaxseed bioactive agent. ConclusionOur data suggests PKM2 as a putative biomarker for IBD and the dysbiosis of microflora in CD. Investigations involving larger number of clinical patients are necessary to validate its use as a serum biomarker of IBD.

ВИПАДОК ПАРАНЕОПЛАСТИЧНОЇ БЕШИХИ

Мета роботи – привернути увагу лікарів до можливості розвитку особливої форми бешихи, що супроводжує онкопатологію, – паранеопластичної.Висновки. Онкопатологія здатна суттєво модифікувати клініку бешихи, зокрема призводити до зростання ступеня інтоксикації, площі ураження і навіть стати причиною «нетипової» локалізації запалення. Це підтверджує доцільність виділення особливої клінічної форми бешихи – паранеопластичної. Цінну інформацію для діагностики цієї хвороби надає термографічне дослідження. Зміни на термограмі зберігаються значно довше, ніж відповідні клінічні прояви, що дає можливість виявити формування хронічної форми і прогнозувати рецидиви.

Correlation between microscopic localization of inflammation in frozen sections and subsequent site of nonmelanoma skin cancer

Correlation between microscopic localization of inflammation in frozen sections and subsequent site of nonmelanoma skin cancer

Localization of Inflammation in the Epileptic Rat Brain by Bi-Functional Fluorescent and Magnetic Nanoparticles (P5.245)

Localization of Inflammation in the Epileptic Rat Brain by Bi-Functional Fluorescent and Magnetic Nanoparticles (P5.245)

Serum biomarkers and source of inflammation in acute coronary syndromes and percutaneous coronary interventions.

There is robust information that confirms the enormous contribution of inflammation to plaque development, progression and vulnerability. The presence of plaques with inflammatory components associates with a greater likelihood of future cardiovascular events. The inflammatory cascade has been implicated during the entire plaque formation, from the early stages of endothelial dysfunction to the development of acute coronary syndromes (ACS). The presence of macrophages, T lymphocytes, dendritic cells, and mast cells in atherosclerotic lesions; the detection of HLA class II antigen expression; and the finding of secretion of several cytokines point to the involvement of immune inflammatory mechanisms in the pathogenesis of atherosclerosis. Serum biomarkers reflecting the activity of biological processes involved in plaque growth or destabilization may provide great help in establishing the appropriate clinical management, and therapeutic interventions. Evidence for a role of inflammation in plaque rupture has been demonstrated by localization of inflammation at plaque rupture sites. However, the focus of inflammation may not precisely reside within the coronary vessel itself but rather in the injured myocardium distal to the disrupted plaque. These observations outline the potential benefits of therapies targeting inflammation in the arterial wall and cardiovascular system. Emerging anti-inflammatory approaches to vascular protection have the potential to benefit patients by marked reductions in serum biomarkers of inflammation and reduce vascular events. With ongoing technical advances, percutaneous coronary interventions (PCI) will continue to play a critical role in the evaluation of novel compounds designed to modulate inflammation. The constant refinements in the different therapeutic strategies, the combination of scientific understanding in the adequate utilization of novel inflammatory markers, the new pharmacologic agents, and the new techniques in PCI will ameliorate our therapeutic management in ACS based on medical evidence.

Diagnostic value of determination of urine cytokines in children with urinary tract diseases

Objective: to determine the urinary concentrations of cytokines for the diagnosis of the localization of inflammation in the urinary system and to establish the contribution of mediators of the immune system to the pathogenesis of the pathology in question. Twenty-nine children aged 2.7 to 11.5 years with pyelonephritis (n=16) and the unspecified localization of urinary infection or cystitis (n=13) were examined. A control group consisted of 15 healthy children. Cytokine levels were determined in the morning urine portion using the multiplex xMAP technology and then recalculated to the concentration of creatinine. Urinary tract infection was accompanied by an avalanche-like increase in the total urinary cytokine concentration, which was 20-fold greater than the reference values in the children with pyelonephritis. Interleukin 8 (IL-8) constituted the highest proportion of cytokines. IL-10 and interferon-γ (IFN-γ) made up the smallest percentage in the total mass of cytokines. Higher IL-6 and IFN-γ values and a simultaneous increase in the level of 2 or more cytokines are of the greatest diagnostic value in determining the renal site of urinary tract infection.

In vivo evaluation of inflammatory bowel disease with the aid of μPET and the translocator protein 18 kDa radioligand [18F]DPA-714.

PurposeThe purpose of the study was to validate [18F]DPA-714, a translocator protein (TSPO) 18 kDa radioligand, as a probe to non-invasively quantify the inflammatory state in inflammatory bowel disease (IBD) animal models.ProceduresQuantitative positron emission tomography (PET) imaging of intestinal inflammation was conducted with 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) a glucose metabolism surrogate marker and [18F]DPA-714 a ligand of the 18 kDa TSPO, on two IBD models. The first model was induced using dextran sodium sulfate (DSS), creating global inflammation in the colon. The second model was induced by rectally administering trinitrobenzenesulfonic acid (TNBS), creating local and acute inflammation.ResultsThe level of inflammation was analyzed using PET imaging on days 7 and 8. The analysis obtained with [18F]DPA-714, yielded a significant difference between the DSS treated (0.50 ± 0.17%ID/cc) and non-treated rats (0.35 ± 0.15%ID/cc). [18F]FDG on the other hand did not yield a significant difference. We did observe a mean glucose consumption in the colon increase from 0.40 ± 0.11 %ID/cc to 0.54 ± 0.17 %ID/cc. In the TNBS model, the uptake level of [18 F]DPA-714 increased significantly from 0.46 ± 0.23%ID/cc for the non-treated group, to 1.30 ± 0.62%ID/cc for those treated. PET signal was correlated with increased TSPO expression at cellular level.ConclusionsResults indicate that [18F]DPA-714 is suitable for studying inflammation in IBD models. [18F]DPA-714 could be a good molecular probe to non-invasively evaluate the level and localization of inflammation. Moreover, in vivo imaging using this TSPO ligand is potentially a powerful tool to stage and certainly to follow the evolution and therapeutic efficiency at molecular level within this disease family.

Acute Paraproctitis. Review of the Literature

Acute anal abscess remains the issue of the day of modern surgery, which is due to, in particular, its occurrence in 0.5% of the population, 0.5-4% of surgical and 10-50% of coloproctological patients. In the article, the analysis of the literature on the etiology, classification, clinical picture, diagnosis and treatment of acute anal abscess is performed. The review revealed that 98% of acute anal abscess is caused by mixed microflora - staphylococci, streptococci in combination with E. coli. The clinical picture of acute anal abscess depends on the localization of inflammation, the virulence of microflora and the immunoreactivity of the organism. Diagnostic methods of acute anal abscess are finger and bidigital rectal examination, anoscopy, rectal examination with a mirror and rectoromanoscopy. Endorectal electrical thermometry, ultrasonography, abscessography, chromoabscessography, computed tomography, magnetic resonance imaging and others can be used as well. Once the diagnosis of acute anal abscess is established, urgent surgical intervention must be performed, which consists of the incision and drainage of abscess with the possible elimination of the opening in the zone of the anal crypt. The postoperative management of patients with acute anal abscess may involve the use of ultrasound, laser, phototherapy, oxygen and ozone therapy, wound dialysis, hydrophonophoresis, multicomponent drugs and others. Thus, as the data in the literature reveals, many questions concerning the management of patients with acute anal abscess are not resolved to the full extent. This is evidenced by the development of inflammatory complications in 13-20%, relapses in 1.5-40%, anal sphincter deficiency in 1.5-27.9% of cases, which makes it worthwhile to continue research on developing new methods of treatments for acute anal abscess