Local Genotypes Research Articles

Some Chemotaxonomic Characteristics of Local Garlic Genotypes

This study was carried out to determine some chemotaxonomic characteristics such as volatile sulfur compounds, mineral content and % protein of 7 garlic genotypes grown in Hatay province. The results revealed that the garlic genotypes have a large variation in terms of sulfur compound, mineral and % protein content. A total of 10 volatile sulfur compounds were detected, mainly diallyl disulphide and its amount in the genotypes varied between 41.51% and 59.48%. The highest value (59.48%) was detected in genotype Altınözü 2. The mineral content of determined in garlic genotypes also differed among genotypes. Genotypes Samandağ 2, Samandağ 3 and Hatay 1 had higher value in terms of Cu, Samandağ 1, Hatay 1 and Hatay 2 had higher value in terms of Zn, Samandağ 2, Altınözü 2, and Hatay 1 had higher value in terms of Na, Samandağ 1, Hatay 1, and Samandağ 3 had higher value in terms of K, Samandağ 1 had higher value in Ca and Fe, Altınözü 2 had higher value in terms of Mn, and Samandağ 2, Hatay 1, and Hatay 2 had high value in terms of Mg. The % protein content of the garlic genotypes varied between 4.77% and 7.71%. It is thought that this study will form a basis for the evaluation of these genotypes in breeding programs.

Performance of French Bean Genotypes at Late Season Sowing in Sylhet, Bangladesh

Performance of four French bean genotypes were evaluated at late sowing condition in Randomized Complete Block design with three replications at the experimental field of Sylhet Agricultural University, Bangladesh to assess the yield performance of French bean genotypes under late-season sowing conditions in Sylhet, Bangladesh. Seeds of four French bean genotypes including BARI Jharsheem-1, Jharsheem-2, Jharsheem-3 and Local were sown in the main field at 25 November and 25 December 2022. The highest number of pods per plant was found in the genotype BARI Jharsheem-1 (32.14), which was statistically at par with the genotype BARI Jharsheem-2 (30.46). The lowest number of pods per plant was found in the genotype BARI Jharsheem-3 (20.10) which was closer to local genotypes (21.68). The highest pod yield per plant was found in BARI Jharsheem-1 (194.45 g) and the lowest in BARI Jharsheem-2 (125.62 g) which was statistically similar with the remaining two genotypes. The highest yield per decimal was produced by the genotype BARI Jharsheem-1 (95.04 Kg) but the lowest yield per decimal was produced by BARI Jharsheem-2 (61.41 Kg). Significant variation was found of sowing date on yield and yield attributes of French bean except some parameters. Maximum yield per decimal was produced from 25 December sowing (73.57 Kg) than 25 November sowing (68.09 Kg). However, interaction effect showed appreciable amount of green pods from BARI Jharsheem-1 at 25 November (87.17 kg/decimal) and 25 December sowing (102.91 kg/decimal) indicating bright scope during late winter season. The results conclude that among the genotypes, BARI Jharsheem- 1 had the highest pod yield and yield per decimal. Sowing on December 25 produced higher yields (73.57 kg/decimal) than November 25 (68.09 kg/decimal). These findings suggest the potential to improve French bean yield and market availability during late winter through optimized genotype selection and sowing dates.

A mammalian herbivore selects local and cooler provenance trees in a restoration common garden experiment: implications for community‐assisted migration efforts

Rapid changes in climate have the potential to alter habitats faster than evolution may respond, leading to maladapted tree populations. Assisted migration may help foundation tree species persist in future climates by identifying populations and genotypes that are robust to expected climate change‐induced alterations. Importantly, community‐assisted migration accounts for impacts of those populations and genotypes on the broader community, including herbivores, often adapted to local plants. These impacts have been examined in arthropod communities, but few studies have assessed mammals, and fewer still have leveraged an experimental design to disentangle genetic contributions to herbivore selection among genotypes and populations. We tested whether North American porcupine (Erethizon dorsatum) browsing on Fremont cottonwood (Populus fremontii) is under genetic control in a common garden to uncouple genetic and environmental contributions on browse selection. Porcupines selected trees from climatically similar and cooler areas, where trees from cooler climates suffered greater than 2× more herbivory than trees from warmer areas. Plant genotype was a significant factor for selection, with the most heavily browsed genotype having greater than 10× more herbivory than the least browsed. Broad‐sense heritability of porcupine tree selection was H2B = 0.27 among genotypes, indicating a genetic component to tree defenses against porcupine herbivory that can be predicted by source population climate. Our results have important implications for future mammalian herbivore populations, should climate change render local tree genotypes maladaptive. We recommend assisted migration efforts plant stock from areas up to 3°C warmer and climatically similar areas to maintain productivity and mammalian herbivore browsing.

Positive Effect of Camelina Intercropping with Legumes on Soil Microbial Diversity by Applying NGS Analysis and Mobile Fluorescence Spectroscopy

Camelina (Camelina sativa (L.) Crantz) is a valuable source of essential amino acids, especially sulphur-containing ones, which are generally lacking in leguminous crops, thus representing an alternative source of protein for both humans and farm animals. Rhizosphere soil samples from five experimental plots with mono- and mixed cultivations of three camelina cultivars, including two introduced varieties Cs1.Pro (Luna) and Cs2.Pro (Lenka) and one Bulgarian variety Cs3.Pro (local Bulgarian landrace) with variety 666 of vetch (Vicia sativa L.) (Cs3-Vs.Pro) and variety Mir of pea (Pisum sativum L.) (Cs3-Ps.Pro), were collected and analysed. The total DNA was isolated from the rhizosphere soils and the presence of the 16S rRNA gene was confirmed by amplification with the universal primer 16SV34. In the present study, the structure of the soil bacterial community in five different plots (Cs1.S.Pro, Cs2.S.Pro, Cs3.S.Pro, Cs3.Vs.S.Pro, and Cs3.Ps.S.Pro) where camelina was grown alone and by being intercropped with pea and vetch was analysed via a metagenomic approach. The number of observed species was highest in the local genotype of the camelina Cs3 grown alone, followed by soil from the intercropped variants Cs3-Vs and CsS-Ps. The soil bacterial communities differed between the sole cultivation of camelina and that grown with joint cultivation with vetch and peas, indicating that legumes considerably affected the growth and development of beneficial microorganisms by aspects such as nitrogen fixing, levels of nitrifying bacteria, and levels of phosphorus-dissolving bacteria, thus helping to provide better plant nutrition. The α-diversity indicated that bacterial communities in the rhizosphere were higher in soils intercropped with vetch and pea. The optical properties of cereals and legumes were determined by their energy structure, which includes both their occupied and free electronic energy levels and the energy levels of the atomic vibrations of the molecules or the crystal lattice.

Agronomic and Starch Content Studies of Local Adlay Accessions (Coix lacrima jobi - L.) from West Sumatra, Indonesia

Indonesia has a high potential for diverse local food resources, one of which is the plant of adlay. Exploration activities aimed at collecting genetic resources of adlay have been successfully carried out in the province of West Sumatra, Indonesia, resulting in the collection of six local genotypes. The purpose of this research is to obtain information about the agronomic potential and starch content of several local adlay accessions. The study was conducted from April to September 2023 in Limau Manis, Padang City, West Sumatra. The six adlay accessions used in the study are PTA-1, KKD-3, PH-4, BTA-2, GT-2, and TJR-2. The experimental design employed was a completely randomized design with three replications. Based on the research findings, significant differences were observed in both agronomic aspects and starch content among the tested accessions. Adlay with the accession code PH-4 exhibited advantages in terms of harvest maturity and production. The starch content of adlay ranged from 56.64% to 61.26%, with the PH-4 accession having the highest amylopectin content (96.60%), while the highest amylose content was found in the GT-2, TJR-2, and KKD-3 accession (19.90%, 20.03%, and 20.48%). Accession PH-4 can be proposed as a candidate for Adlay variety assembly. Keywords: agronomic, starch content, adlay accessions, Coix lacrima jobi

Ampelographic Diversity Assessment of Local Grape Genotype Grown in India

Grapevine is well adapted to the agro climatic conditions of India, which makes it one of the major element of the Indian horticulture. However, there has been limited focus on assessing grapevine germplasm, and genetic resources have previously remained underutilized despite their potential for adapting to environmental changes. This study aims to evaluate the diversity of local (developed in India) grapevine accessions growing in different regions in India. A total of 20 local varieties were evaluated using 14 fruit traits previously developed by DUS. An important variability was revealed among the local varieties studied based on fruit characteristics. The most discriminant traits were bunch weight, berry diameter, bunch length, berry weight, berry shape, seed number per berry. Hierarchical clustering analysis showed three main clusters, each regrouping accessions of different named varieties. Mean values of cluster for various yield parameters revealed that cluster I possessed maximum value for yield related attributes. Maximum bunch weight was observed in Manjari Medika (270.0g) followed by Pusa Seedless (243.1 g), Arka Shyam (213.5 g) while higher 50 berry weight (201.0 g) and berry diameter (20.30 mm) in Arka Majestic genotype. Arka Majestic and Manjari Medika showed better quality traits. Our results indicate a potential of morphological diversity within the local genotypes that should be further investigated in order to understand their performance and to evaluate them in selection programs.

Study of Genetic Diversity of Aromatic Rice Genotypes Using Random Amplified Polymorphic DNA Markers

The study of genetic variation in aromatic rice is necessary for enhancing and preserving its genetic makeup. In this study, we amplified four Random Amplified Polymorphic DNA (RAPD) markers from the genomes of 11 different aromatic rice genotypes in order to evaluate the genetic variation and relatedness in aromatic rice. The genotypes were collected from different sources- five of which were obtained from the Bangladesh Rice Research Institute, one from the Bangladesh Institute of Nuclear Agriculture, and the remaining five were local genotypes. In RAPD profiling, twenty-seven of the thirty-six bands that were generated by the four primers were polymorphic, resulting in an estimated average polymorphism of 47.22 percent. The average value of the polymorphic information content was 0.309 with a range from 0.165 to 0.456, indicating that the primers were effective to detect genetic diversity between the genotypes. The genetic diversity and Shannon information index across all of the genotypes and primers were 0.176 and 0.260, respectively, which indicated that there was a certain amount of genetic variation in the studied aromatic rice genotypes. Kalizira and Binadhan-9 genotypes had the smallest genetic distance (0.057), while it was the highest (0.406) between the BRRI dhan 34 and BRRI dhan 50. The genotype pairs BRRI dhan 38 vs BRRI dhan 34, BRRI dhan 38 and Kalizira, and Zirakatari and Kalizira had also a higher level (0.365) of genetic distances. The genotypes were grouped into two main clusters in the UPGMA dendrogram. Cluster I included five genotypes: BRRI dhan 34, Basmoti, Atashail, Kalizira, and Binadhan-9. Cluster II included remaining six genotypes: BR5, BRRI dhan 38, BRRI dhan 50, Uknimodhu, BRRI dhan 37, and Zirakatari. Documentation of the genetic variation and relatedness among the aromatic rice genotypes under this investigation could be useful for future research into the development of aromatic rice and related fields. J Bangladesh Agril Univ 22(3): 277-284, 2024

Early Life History Divergence Mediates Elevational Adaptation in a Perennial Alpine Plant

ABSTRACTSpatially divergent natural selection can drive adaptation to contrasting environments and thus the evolution of ecotypes. In perennial plants, selection shapes life history traits by acting on subsequent life stages, each contributing to fitness. While evidence of adaptation in perennial plants is common, the expression of life history traits is rarely characterized, limiting our understanding of their role in adaptive evolution. We conducted a multi‐year reciprocal transplant experiment with seedlings from low and high elevation populations of the alpine carnation Dianthus carthusianorum to test for adaptation linked to contrasting climates and inferred specific contributions of early life stages to fitness. We assessed genotype by environment interactions in single fitness components, applied matrix population models to achieve an integrated estimate of fitness through population growth rates, and performed trade‐off analyses to investigate the advantage of alternate life history traits across environments. We found evidence of genotype by environment interactions consistent with elevational adaptation at multiple stages of the early life cycle. Estimates of population growth rates corroborated a strong advantage of the local genotype. Early reproduction and survival are alternate major contributors to adaptation at low and high elevation, respectively, and are linked by trade‐offs that underlie the evolution of divergent life history traits across environments. While these traits have a strong genetic basis, foreign populations express co‐gradient plasticity, reflecting the adaptive strategy of the local populations. Our study reveals that selection associated to climate has driven the evolution of divergent life histories and the formation of elevational ecotypes. While the high energy environment and strong competition favor investment in early reproduction at low elevation, limiting resources favor a more conservative strategy relying on self‐maintenance at high elevation. The co‐gradient plasticity expressed by high‐elevation populations may facilitate their persistence under warming climatic conditions.

Genetic Variability, Heritability, Genetic Advance, Path Coefficient Analysis, and Inter-Character Relationships in Colocynth (Citrullus colocynthis [L.] Schrad.) from Southeastern Villages of the Benin Republic

Genetic improvement of crops for important traits requires reliable estimates of genetic variability. heritability and genetic advance of intending parent materials to identify traits useful in planning an efficient breeding program through selection. Hence, the effectiveness of genetic improvement of a crop depends on the variability in the morpho-agronomic traits of individual genotypes. Understanding the variation that exists will allow the breeder to determine strategies to be adopted in his breeding program. The objectives of the present study were to estimate the magnitude of genetic variability and advance, heritability and inter-character relationships by simple correlations and path coefficient analysis in 40 local colocynth genotypes from Southeastern Benin. The experiment was carried out in a randomized complete block design with three replications in three locations for three years. 34 morpho-agronomic traits were observed in each genotype. Analysis of variance revealed that effects of genotype, genotype by year or location and genotype by year by location interactions were significant (p ≤ 0.01 or 0.05) for all the characters. Phenotypic coefficients of variation (PCV) values were relatively greater than genotypic coefficients of variation (GCV) for all traits. High magnitude of phenotypic and genotypic coefficients of variation as well as high heritability along with high genetic advance were recorded particularly for qualitative traits. All yield components, except time to emergence, time to tailspins, leaf limb width, fruit number per plant, fruit width, seed length, seed tegument percentage and stem pubescence texture, had significant and positive correlations with yield attributes represented by thousand-seed weight (TSW) and seed number per plant (SNP). Path coefficient analysis indicated that all the characters (except leaf pubescence texture, stem pubescence texture, fruit design produced by secondary skin color and male flower size) had positive direct effects on TSW and partly SNP. Results suggested that most of the 34 quantitative and qualitative traits studied could be effectively used as selection criteria in the breeding program of Citrullus colocynthis varieties with high yield.

Factors Affecting the Quality of Ethiopian Coffee (Coffea Arabica L.)

Ethiopia is fortunate in this regard, as it is home to many different types of. Arabica coffee (Coffea arabica L.) is the main coffee variety in global coffee production, accounting for more than 60% of total production. Arabica coffee accounts for 30% of Ethiopia's foreign exchange earnings. Arabica coffee is a popular coffee variety in coffee producing and consuming countries due to its unique aroma and taste and lack of caffeine. Ethiopia's most important export is coffee, which is also its most important crop. Ethiopia is well known for producing very high-quality coffee, renowned for its excellent aroma and taste, and is also the birthplace of Arabica coffee. The coffee industry attaches great importance to coffee quality. Cup quality is a complex trait that depends on many factors, including species or variety (genetic factors), environmental conditions (ecological factors), agronomic practices (cultivation factors), processing systems (post-harvest factors), storage conditions, and industrial factors. process, beverage preparation and consumer preferences. However, the quality of coffee produced by Ethiopian farmers sometimes deteriorates. Additionally, local genotype, climate and soil characteristics, agricultural practices, harvesting strategy and timing, post-harvest handling procedures, grading, packaging, storage and transportation conditions all affect coffee quality, either improving or decreasing coffee quality.

Abstract A079: The impact of Indigenous American ancestry on the risk of acute lymphoblastic leukemia and the implications for future study designs

Abstract Acute lymphoblastic leukemia (ALL) is the most common childhood cancer, with Hispanic/Latino (H/L) children having up to 1.4 times the rate of ALL compared to their non-Hispanic White counterparts. This disparity has not been fully explained by environmental factors, suggesting the role of understudied genetic variants, particularly ones found in Indigenous American (IA) ancestry inherited by Latinos. In this study, we characterized the impact of IA ancestry on the frequency and effect size of known ALL risk alleles. We found that elevated risk of ALL in Latinos may be conferred by increased frequency of risk alleles due to IA ancestry. For instance, in an analysis of Latinos from the California Cancer Linkage Project (CCRLP; 1,930 cases, 2,103 controls) and the California Childhood Leukemia Study (CCLS; N = 605 cases, 515 controls), we found that, across independent known ALL loci (N=21), more loci than expected by chance showed a significant association between IA ancestry and increasing risk (p = 0.015). Compared to randomly-sampled, frequency-matched alleles from the genome, the risk alleles at all known ALL loci also showed a significant association with IA ancestry (46.9 % for known risk alleles vs. 43.5 % for matched alleles; ). The IA haplotype had 1.39 times the odds (95% CI: 1.35 – 1.43; ) of harboring the risk allele compared to the non-IA haplotype. Conversely, in a combined Latino sample of 2,535 cases and 9,035 controls, we found no evidence of GxAncestry interaction on ALL risk across all known loci (min P = 0.0026, prior to adjusting for multiple testing), suggesting that ALL risk alleles do not have increased effects on the IA ancestry background. This lack of synergism between local ancestry and genotype in ALL has methodological implications for gene discovery in admixed populations. To explore this, we implemented Tractor, a method that models genotype effects by ancestry, and thus is best powered in the presence of effect size heterogeneity by ancestry. Across known loci, we found that Tractor had 36.1% decreased power compared to standard GWAS. Taken together, our results suggest that the disproportionate burden of ALL in Latino populations may arise from the enrichment of risk alleles within the IA ancestry component, possibly driven by immunity- related selective pressures. Finally, future genetic studies of ALL in Latino patients may benefit from methods that incorporate contributions from ancestry to increase the statistical power in identifying associations, rather than leverage effect size heterogeneity across ancestries. Citation Format: Jalen Langie, Libby Morimoto, Xiaomei Ma, Catherine Metayer, Joseph L. Wiemels, Adam J. de Smith, Charleston W.K. Chiang. The impact of Indigenous American ancestry on the risk of acute lymphoblastic leukemia and the implications for future study designs [abstract]. In: Proceedings of the 17th AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2024 Sep 21-24; Los Angeles, CA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2024;33(9 Suppl):Abstract nr A079.

Phenological, Morphological and Pomological Characteristics of ‘Samsun Güzeli’ Pear Genotype on BA29 Rootstock

Türkiye has many local pear cultivars; some of them do not have much production and propagation potential due to their low quality and undesirable vegetative and generative characteristics. However, those that are superior in terms of fruit quality and yield characteristics have found a place for themselves in both local and national markets. In this respect, the ‘Samsun Güzeli’ genotype, which is an important local cultivar, is an ideal cultivar with many features and has a high potential to spread its cultivation all over Türkiye and other countries. ‘Samsun Güzeli’ is an autumn genotype, can easily meet the high demand in local and international markets due to its attractive color, smooth fruit shape and high quality. Despite all these valuable features, this cultivar is still not well known in Türkiye. There is a great need to disseminate cultivation and research on this subject. This study mainly aimed to reveal the phenological, morphological and pomological characteristics of the ‘Samsun Güzeli’ local pear genotype grafted on BA29, a commercially traded rootstock in 2021-2022. The results showed that the ‘Samsun Güzeli’ pear genotype completed its phenological cycle in 255 days in 2021 and 228 days in 2022 in Samsun climatic conditions. Fruit set rate of the genotype was 12.05% and the average yield was 17306.23 kg ha-1. It was also determined that the morphological characteristics averages were as the following; rootstock diameter 76.71 mm, trunk diameter 61.97 mm, trunk cross-sectional area 34.06 cm2, tree height 253.12 cm, canopy volume 0.96 m3, annual shoot length 37.19 cm and leaf area 11.74 cm2. Averages values of pomological and chemical properties recorded as the following; fruit weight 114.60 g, fruit volume 109.90 ml, soluble solid content (SSC) 13.16%, acidity 0.33%, and pH 4.37. In the sensory evaluations, the highest scores were obtained by juiciness (6.32) in the year 2022 while scores of overall visual quality were lowest in both years. In conclusion, it can be said that the ‘Samsun Güzeli’ genotype performs adequately in terms of fruit yield and quality on the standard dwarf rootstock and conducting new studies might be beneficial to disseminate the genotype.

Characterization of some local and commercial bread wheat (Triticum aestivum L.) genotypes with allele-specific DNA markers

Landraces play a significant role as genetic reservoirs in wheat breeding endeavors. Advances in functional marker technology have facilitated early and more precise selection processes. This study involved the characterization of a total of 96 bread wheat genotypes, comprising 76 landraces and 20 registered cultivars, utilizing allele-specific DNA markers targeting various genes including those for gluten strength, yellow rust resistance, stem rust resistance, dwarfism, rye translocation, hardness, and softness. Molecular analysis revealed the presence of 148 alleles, with an average of 21.14 alleles per marker, and an average polymorphic information content (PIC) value of 0.5625. Specific genes such as the rye translocation gene were identified in genotypes 161 and 884, while the hardness gene was found in genotypes 672, 3088, 3384, 3414, and 3541. Stem rust resistance gene was detected in the cultivar Adana-99, yellow rust resistance gene in genotypes 1635 and 2115, and the softness gene in 31 genotypes including the cultivar Masaccio. Based on the dendrogram analysis, genotype 3652 exhibited around 93% genetic similarity with the cultivar Masaccio, while genotypes 2190, 2715, and 2897 showed similarity to genotype 2946. Genotypes 2959 and 2960 and genotypes 3334 and 3359 shared approximately 91% genetic similarity.

Assessing Heat Stress Tolerance of Wheat Genotypes through Integrated Molecular and Physio-Biochemical Analyses

Heat as an abiotic stress significantly impairs the sustainable productivity of wheat (Triticum aestivum L.). To determine the tolerance of genotypes to heat stress, a comprehensive approach should be used that integrates simultaneous phenotyping and genotyping analyses. The aim of this study is to identify local heat-tolerant genotypes using simple sequence repeat (SSR) markers and evaluate the selected genotypes under field conditions for their tolerance to heat stress. Of the 12 SSR markers that showed polymorphism, eight were associated with six important traits. The use of hierarchical cluster analysis (HC) based on SSR markers led to the identification of 13 genotypes that showed varying results and were grouped into three distinct heat tolerance classes: tolerant (T), moderately tolerant (MT), and sensitive (S). The results showed that heat stress had a significant effect on 19 traits under this study, with significant variation in tolerance to heat stress between genotypes. The tolerant genotypes exhibited a range of average thousand-kernel weight (TKW) values between 40.56 and 44.85, while the sensitive genotype (Yecora Rojo) had an average TKW of 35.45. Furthermore, the tolerant genotypes showed two to three times higher levels of antioxidants compared to the sensitive genotypes when exposed to heat stress. Among the traits analyzed, six showed a favorable combination of high heritability (>60%) and genetic gain (>20%). Through the integration of principal component analysis and stepwise multiple linear regression, it was determined that six traits (grain yield, 1000-kernel weight, plant height, intercellular carbon dioxide, flag leaf area, and grain filling duration) revealed differences between the 13 genotypes. HC analysis of the six traits resulted in the same division of genotypes into three main categories as observed in an HC analysis based on SSR markers. It is worth noting that Saudi wheat, including KSU106, KSU105, and KSU115 as local genotypes, in addition to the 16HTWYT-22 genotype, showed higher heat tolerance compared to the other genotypes tested, indicating its potential suitability for agriculture in Saudi Arabia. These results contribute to breeding programs focused on developing heat-tolerant wheat varieties and accelerate progress in wheat productivity improvement programs.

EKSPLORASI KERAGAMAN PLASMA NUTFAH PADI LOKAL KUANTAN SINGINGI BERDASARKAN MORFOLOGI GABAH DAN BERAS

Farmers in specific region can play a crucial role in preventing the extinction of rare rice varieties by maintaining their commitment to local rice production. This not only contributes to the economic stability of the community but also necessitates exploration efforts to identify and gather specific plant species at risk of extinction. This study employs a descriptive research design to identify, gather, and compare the relationships between local rice genotypes in the Kuantan Singingi regency. The survey method utilizes a planned sample of rice seeds.The exploration process involves observing geographic coordinate locations, collecting local rice seeds, and examining grain and rice morphology based on Bioversity International, IRRI, and WARDA standards from 2007. The Kuantan River, with an altitude ranging from 28.95 to 61.56 meters above sea level, is prone to frequent flooding. A morphological study of grains reveals that, out of 26 local rice genotypes, one pair exhibits the highest similarity rate of 71% when compared to more diverse types. Notably, the white sironda rice from Pulau Madina village in Kuantan Hilir district and pandan wangi rice from Pauh Angit village in Pangean district are identified as the most identical genotypes. By identifying and preserving unique and highly-potential indigenous rice genotypes, this research significantly contributes to the enhancement of food stability, rural economy, and genetic resilience of rice plasma in Indonesia.

Growth Performance, Meat Quality, and Lipid Oxidation in Pigs’ Fed Diets Containing Grape Pomace

Grape pomace is a winery byproduct that is rich in polyphenols with antioxidant capacity. This study investigated the effect of 0, 5, and 10% inclusion of dehydrated grape pomace (DGP) in finishing pig diets on the growth performance, carcass traits, fatty acid profile, fresh meat quality, and fat stability of a local pig genotype. A total of 36 pigs, 18 barrows, and 18 gilts (83.23 ± 6.03 kg and 132.1 ± 5.6 days old) were allotted in a randomized block design considering the initial weight (block) within sex, with six replicates of each sex per treatment. Including DGP in the diets did not affect daily weight gain or the feed-to-gain ratio; however, daily feed intake increased linearly (p < 0.05) and backfat thickness at the last rib, backfat thickness at the first sacral vertebrae, P2 backfat thickness, fat area, and the percentage of lean meat decreased linearly (p < 0.05) in pigs. The inclusion of DGP in pig diets did not affect the antioxidant potential evaluated by thiobarbituric acid-reactive substances in mini hamburgers or the quality characteristics of fresh meat, except for intramuscular fat (EE). The dietary inclusion of DGP linearly increased (p < 0.05) EE, saturated fatty acids, monounsaturated fatty acids, and Σω-3 and reduced the ω-6:ω-3 ratio in a linear way (p < 0.05) in the loin of pigs. We concluded that it is feasible to include up to 10% of DGP in pig diets without affecting growth performance, but carcass quality may be impaired due to increased adiposity. Furthermore, meat quality can be improved by increasing intramuscular fat and ω-3 fatty acid content, but fat stability is not affected when DGP is included at up to 10% of the diet for 49 days prior to slaughter.

Morphological Differentiation, Yield, and Cutting Time of Lolium multiflorum L. under Acid Soil Conditions in Highlands.

Livestock production in the basins of the northern macro-region of Peru has as its primary source pastures of Lolium multiflorum L. 'Cajamarquino ecotype' (ryegrass CE) in monoculture, or in association with white clover Ladino variety, for feeding. The objective of this research work was the morphological characterisation, yield evaluation, and cutting time evaluation of two local genotypes (LM-58 and LM-43) of Lolium multiflorum L. in six locations. An ANOVA was performed to compare fixed effects and interaction. It was determined that the LM-58 genotype is intermediate, growing semi-erect, with a dark green colouring and 0.8 cm broadleaf, and can reach an average stem length of 46 cm, up to 1.6 cm. day-1, achieving fourth-leaf growth at 28 days under appropriate management conditions. Despite the differentiated characteristics, according to BLASTn evaluation, the ITS1 sequences showed a greater than 99.9% similar identification to Lolium multiflorum L., characterising it as such. It was determined that the LM-58 genotype outperforms LM-43, achieving a forage yield of 4.49 Mg. ha-1, a seed production of 259.23 kg. ha-1, and an average of 13.48% crude protein (CP). The best biomass yield (49.10 Mg. ha-1.yr-1) is reached at 60 days; however, at 30 days, there is a high level of CP (14.84%) and there are no differences in the annual protein production at the cutting age of 60 and 45 days. With the results of the present study, LM-58 from a selection and crossbreeding of 680 ryegrass EC accessions emerges as an elite genotype adapted to the conditions of the northern high Andean zone of Peru.

African Local Pig Genetic Resources in the Context of Climate Change Adaptation.

Africa is home to a wide diversity of locally adapted pig breeds whose genetic architecture offers important insights into livestock adaptation to climate change. However, the majority of these inherent traits have not been fully highlighted. This review presents an overview of the current state of African pig genetic resources, providing highlights on their population and production statistics, production system, population diversity indices, and genomic evidence underlying their evolutionary potential. The study results reveal an incomplete characterization of local pig genotypes across the continent. The characterized population, however, demonstrates moderate to high levels of genetic diversity, enough to support breeding and conservation programs. Owing to low genetic differentiation and limited evidence of distinct population structures, it appears that most local pig populations are strains within larger breeds. Genomic evidence has shown a higher number of selection signatures associated with various economically important traits, thus making them potential candidates for climate change adaptation. The reportedly early evidence of hybridization with wild suid groups further suggests untapped insights into disease resistance and resilience traits that need to be illuminated using higher-density markers. Nevertheless, gene introgression from commercial breeds is prevalent across Africa; thus, efforts to realize and utilize these traits must increase before they are permanently depleted.

Evaluation of Chemical Compounds in Local Garlic Genotypes from Southwestern Romania

Garlic (Allium sativum L.) is one of the most esteemed plants due to its medicinal properties. Its health benefits for humans are attributed to its chemical compounds. Few studies characterize garlic genotypes cultivated in Romania concerning their chemical composition. In this context, this study aimed to determine the chemical compounds for 16 local garlic genotypes cultivated under the same climatic and technological conditions. The chemical characteristics studied were the total phenolic content (TPC), total flavonoid content (TFC), reducing sugar content, antioxidant activity (AO), vitamin C, and acidity level. Chemical composition varied significantly among genotypes: TPC ranged from 656.07 to 1317.32 µg GAE/g FW; TFC ranged from 427.08 to 1447.90 µg QE/g FW; vitamin C ranged from 3.24 to 5.37 mg/g FW; reducing sugar content ranged from 3.24 to 5.37 mg/g FW; and the acidity level for the control was 6 meq/100 g. Among the selected genotypes, differences were observed between 3.48 and 11.02 meq/100 g. Significant correlations were noted between different compounds, specifically between TPC and AO, as well as between TPC and acidity level. In conclusion, this study highlights significant variability in terms of chemical composition of local garlic genotypes, which indicates notable differences between them and suggests that the genotypes may have different potential in medicinal and nutritional uses due to their distinctive chemical compositions.

Mecanismos adaptativos durante la recuperación de genotipos de uvas locales tolerantes y sensibles sometidos a estrés salino

Utilization of distinct genetic resources is an auspicious prospective strategy to combat adverse impacts of salinity, which is expected to get worse under climate change conditions, for maintaining grape production and quality. This research aims to study the adaptive mechanisms during the recovery of tolerant and sensitive salt-stressed local grape genotypes on the bases of biochemical, anatomical and gene expression responses. Transplants of three Egyptian grapes (Vitis vinifera); Baltim Eswid, Romy Ahmer and Romy Abiad, were exposed to sodium chloride-induced salt stress of 2.28 and 3.75 mS compared to 695 µS water-irrigated control for two months, then all plants were irrigated with tap water for additional one month for recovery. Recovered Baltim Eswid cultivar following the highest saline treatment gave maximum survival percentage (100 %), while Romy Abiad recorded the lowest rate (40 %). Suggested adaptive mechanisms include: damage reduction caused by salinity-related oxidative stress, osmotic adjustment, and perform structural modifications that allow protection. It was concluded that, Blatim Eswid is a superior salt-tolerant local grape genotype, while Romy Abiad is the most sensitive as affected mostly by oxidative stress represented by a significant increment of hydrogen peroxide content. Keywords: grape, salinity, recovery, genotypes.