Local Blood Banks Research Articles

Frequency of Duffy, Kidd, Lewis, and Rh Blood Group Antigens and Phenotypes Among Donors in the Al-Ahsa Region, Saudi Arabia.

In Al-Ahsa, Saudi Arabia, the high consanguinity rates contribute to the prevalence of inherited hemoglobinopathies such as sickle cell disease and thalassemia, which frequently require blood transfusions. These transfusions carry the risk of alloimmunization, necessitating a precise blood component matching to mitigate health risks. Local antigen frequency data is vital for optimizing transfusion practices and enhancing the safety of these medical procedures for the Al-Ahsa population. This study investigated the distribution of Duffy, Kidd, Lewis, and Rh blood group antigens in 1,549 individuals from the region; comparing the frequencies with global data. Serological analyses revealed a high prevalence of the Fy(a+b-) and Jk(a+b+) phenotypes in the Duffy and Kidd blood groups, respectively, with Jk(a-b-) being notably scarce. The Lewis blood group exhibited a significant presence of Le(a-b+) and Le(a+b-) phenotypes, whereas Le(a+b+) was less common. In the Rh system, the D antigen was most prevalent, with other antigens following in descending order of frequency. The study underscores the regional variation in antigen frequencies, emphasizing the need for local blood banks to adapt their screening and matching practices to mitigate the risk of alloimmunization and enhance transfusion safety. These findings are pivotal for refining transfusion strategies and understanding the immunohematology landscape in Al-Ahsa.

An overview of cord blood stem cell transplantation in Hong Kong

Haematopoietic stem cell graft derived from cord blood is standard therapy for several haematological malignancies and other diseases. The study reports cases of public and private (family) cord blood biobanking services and the related hematopoietic stem cell transplantation ever performed in Hong Kong. The published original research papers and review articles from inception to Nov 2022 have been searched for on Pubmed, Microsoft Academic Search, and Google Scholar to identify reports on existing or terminated cord blood biobanking and transplantation service in Hong Kong. Moreover, all data publicly available on the official websites of the local cord blood banks and local mainstream media has been analysed. The public Hong Kong Red Cross Blood Transfusion Service delivers the highest quantity of haematopoietic stem cell transplants. Among the private sector, HealthBaby releases the most cord blood units for clinical use in diseases in both autologous and allogeneic administration, followed by Cordlife HK. Both public and private (family) cord blood biobanks have been and continue to contribute to the Hong Kong cord blood donor registry. However, the growth of the cord blood inventory is detrimental to donor-recipient matching for lifesaving therapy.

Time dependent changes in red blood cells during storage in the local blood banks of Khyber Pakhtunkhwa.

This project aimed at determining time-dependent ultrastructural and haematological changes taking place in blood stored in local blood banks of Khyber Pakhtunkhwa. It was a longitudinal study with repeated measures design. Twenty healthy blood donors participated in this study. An amount of 250ml blood was collected from each donor and stored in Citrate Phosphate Dextrose Adenine-1 (CPDA-1)- containing blood bags. Within first four hours, baseline samples were taken while subsequent samples were obtained at 5 days interval till day 20th. Structural changes in RBCs were observed under light and scanning electron microscope (SEM) at different intervals. Furthermore, haematological parameters and osmotic fragility were also determined. Remarkable alterations were seen in RBCs morphology. From 5th day onwards, multiple visible spicules were observed on the RBC's outer membrane and more than 2/3rd cells were abnormal at day 20. There was a significant reduction in RBCs count and haemoglobin concentration while the remaining parameters remained unchanged. Osmotic fragility increased significantly over time, with <1% haemolysis noted in baseline samples as compared to 2.4% haemolysis on day 20th (p≤0.0001). Prolonged storage of blood results in distorted RBCs morphology and increased fragility. Transfusion of such cells would potentially result in rapid lysis in patients with hepatosplenomegaly and conditions requiring multiple blood transfusions.

A Blood Bank Network Design Problem with Integrated Facility Location, Inventory and Routing Decisions

We aim to design an effective supply chain network for a blood distribution system to satisfy the needs of hospitals in a certain region. In the analyzed current system, each hospital keeps a certain level of inventory, received at certain time periods by the shipments from a main blood bank. We propose an alternative system, in which some of the hospitals are selected as local blood banks (LBB) and all other hospitals will be assigned to an LBB. More frequent shipments will be made from LBBs to these hospitals, leading to lower inventory levels to be kept at each hospital. The inventories kept separately at the hospitals in the current system will be pooled at the selected LBBs in the proposed system. We develop a mixed integer nonlinear programming (MINLP) model to determine the optimal selection of LBBs, assignment of hospitals to LBBs, optimal inventory levels at each LBB and routing decisions among the facilities in order to minimize total system costs. We also propose a piecewise linear approximation method and a simulated annealing heuristic approach to find the solution of this problem. The proposed model and the solution techniques are applied on a real life case study for the blood distribution network in Istanbul. It is observed that significant improvements can be obtained by the proposed system when compared to the current design. Performances of the solution methods are also compared and a sensitivity analysis related to system parameters is presented via detailed numerical experiments.

Maintaining Trauma Care Access During the COVID-19 Pandemic: An Urban, Level-1 Trauma Center's Experience.

Maintaining Trauma Care Access During the COVID-19 Pandemic: An Urban, Level-1 Trauma Center's Experience.

The Las Vegas mass shooting: An analysis of blood component administration and blood bank donations.

The deadliest mass shooting in modern United States history occurred on October 1, 2017, in Las Vegas, killing 58 and overwhelming hospitals with more than 600 injured. The scope of the tragedy offers insight into medical demands, which may help guide preparedness for future mass shooting incidents. Retrospective, deidentified, health care institution-provided data from all hospitals and blood banks providing care to Las Vegas shooting victims were gathered. Study authors independently reviewed all data and cross-referenced it for verification. Main outcomes and measures include the number of victims requiring hospital and intensive care admission, the amount and types of blood components transfused during the first 24 hours, and the amount of blood donated to local blood banks following the Las Vegas mass shooting. Two hundred twenty patients required hospital admission, 68 of them to critical care. Nearly 500 blood components were transfused during the first 24 hours in a red blood cell-to-plasma-to-platelet ratio of 1:0.54:0.81. Public citizens donated almost 800 units of blood immediately after the shooting; greater than 17% of this donated blood went unused. The amount of blood components transfused per patient admitted was similar in magnitude to other mass casualty events, and available blood supply met patient demand. The public call for blood donors was not necessary to meet immediate demand and led to resource waste. Preparation for future mass shooting incidents should include training the community in hemorrhage control, encouraging routine blood donation, and avoiding public calls for blood donation unless approved by local blood suppliers. Therapeutic study, level V.

An Emergency Blood Allocation Approach Considering Blood Group Compatibility in Disaster Relief Operations

Large-scale sudden-onset disasters may cause massive injuries and thus place great pressure on the emergency blood supplies of local blood banks. When blood is in short supply, blood products gathered urgently to a local blood center should be appropriately allocated to blood banks in the affected area. Moreover, ABO/Rh(D) compatibilities among blood groups must be considered during emergency situations. To minimize the total unmet demand of blood products considering the optimal ABO/Rh(D)-compatible blood substitution scheme, a mixed integer programming model is developed and solved efficiently by using a greedy heuristic algorithm. Finally, a numerical example derived from the emergency blood supply scenario of the Wenchuan Earthquake is presented to verify the proposed model and algorithm. The results show that considering ABO/Rh(D)-compatible blood substitution can remarkably increase the efficiency of emergency blood allocation while lowering blood shortage, and the preference order of possible ABO/Rh(D)-compatible substitutions has an influence on the allocation solution.

Transfusion in Haemoglobinopathies: Review and recommendations for local blood banks and transfusion services in Oman.

Sickle cell disease and homozygous β-thalassaemia are common haemoglobinopathies in Oman, with many implications for local healthcare services. The transfusions of such patients take place in many hospitals throughout the country. Indications for blood transfusions require local recommendations and guidelines to ensure standardised levels of care. This article summarises existing transfusion guidelines for this group of patients and provides recommendations for blood banks and transfusion services in Oman. This information is especially pertinent to medical professionals and policy-makers developing required services for the standardised transfusion support of these patients.

High-Resolution HLA-A Typing in Normal Iranian Population

Background:Human leukocyte antigen (HLA) gene is a highly polymorphic region. HLA typing is required to match patients and donors for transplantation; therefore, development of HLA registries is necessary for finding HLA match donors. HLA system is highly informative, and numerous studies have been conducted on HLA allele distribution in different populations.Methods:In this study, 100 unrelated Iranian individuals were typed for HLA-A locus using sequence-based typing method. Samples were subjected to the PCR, followed by Sanger sequencing and software analysis.Results:A*02:01 (13%) and A*24:02 (12%) were the two most frequent alleles, while A*01:14, A*02:05, A*02:11, A*02:34, A*02:50, A*11:04, A*23:02, A*24:34, A*25:01, A*26:09, A*26:43, A*29:67, A*30:54, A*31:02, A*31:66, A*32:03, A*32:04, A*33:03, and A*66:15 alleles had the least frequencies (1%).Conclusion:This is the first report of HLA-A allele level typing in a randomized population of Iran and can be useful for development of national registries of HLA-typed volunteer marrow donors and local cord blood banks.

Storage time of intraoperative transfused allogeneic red blood cells is not associated with new-onset postoperative atrial fibrillation in cardiac surgery.

BackgroundAllogeneic red blood cell (RBC) transfusion has been associated with new-onset postoperative atrial fibrillation (POAF) following cardiac surgery. Prolonged storage time of RBC may increase the risk. The primary aim of the study was to evaluate whether the storage time of RBC is associated with development of POAF.Materials and methodsPre-, per- and postoperative data were retrieved from the Western Denmark Heart Registry and local blood banks regarding patients who underwent coronary artery bypass surgery, valve surgery or combined procedures in Aalborg or Aarhus University Hospital during 2010–2014. Multiple logistic regression was used to determine the risk of POAF according to transfusion of RBC on the day of surgery. Furthermore, we determined trend in storage time of RBC according to risk of POAF using restricted cubic splines. Patients with a history of preoperative atrial fibrillation, patients who received transfusions preoperative and patients who died at the day of surgery were among excluded patients.ResultsA total of 2,978 patients with a mean age of 66.4 years were included and 609 patients (21%) received RBC transfusion on the day of surgery. POAF developed in 752 patients (25%) and transfused patients were at an increased risk compared with non-transfused patients (adjusted Odds Ratios for patients receiving RBC: 1.37; 95% CI: 1.11–1.69, P-value = 0.004). However, RBC transfusion was not necessarily the cause of POAF and may only be a marker for development of POAF. There was no significant association between storage time of RBC and POAF.ConclusionsIn contrast to intraoperative allogeneic RBC transfusion in general, increased storage time of RBC is not associated with development of POAF in cardiac surgery.

An Interactive Possibilistic Programming Approach for Blood Bank Locations with Emergency Referral

Regionalisation of Local Blood Banks (LBBs) is so vital for blood supply to facilitate the clinics in the obligated regions to go along with the requests in typical conditions and the moments of crisis. Determination of location of LBBs is a key factor in the selection of alternatives in the blood store networks. In this paper, we first develop a new multi-objective possibility mixed integer linear programming model (MOPMILP) for blood bank location problem considering distinctive contradictive goals at the same time and additionally the loose way of some basic parameters, for example, requirements of hospitals and number of emergency referrals for clinics or hospitals. At that point, in the wake of applying legitimate procedures for changing over this possibilistic model into a precise novel multi-objective linear model (MOLP), we propose a novel intelligent fuzzy method to understand this MOLP and find a proper trade off arrangement. Eventually, we propose a careful analysis and will see the performance of the proposed model.

Poor Prognosis in Warfarin-Associated Intracranial Hemorrhage Despite Anticoagulation Reversal

Anticoagulant-associated intracranial hemorrhage (aaICH) presents with larger hematoma volumes, higher risk of hematoma expansion, and worse outcome than spontaneous intracranial hemorrhage. Prothrombin complex concentrates (PCCs) are indicated for urgent reversal of anticoagulation after aaICH. Given the lack of randomized controlled trial evidence of efficacy, and the potential for thrombotic complications, we aimed to determine outcomes in patients with aaICH treated with PCC. We conducted a prospective multicenter registry of patients treated with PCC for aaICH in Canada. Patients were identified by local blood banks after the release of PCC. A chart review abstracted clinical, imaging, and laboratory data, including thrombotic events after therapy. Hematoma volumes were measured on brain CT scans and primary outcomes were modified Rankin Scale at discharge and in-hospital mortality. Between 2008 and 2010, 141 patients received PCC for aaICH (71 intraparenchymal hemorrhages). The median age was 78 years (interquartile range, 14), 59.6% were male, and median Glasgow Coma Scale was 14. Median international normalized ratio was 2.6 (interquartile range, 2.0) and median parenchymal hematoma volume was 15.8 mL (interquartile range, 31.8). Median post-PCC therapy international normalized ratio was 1.4: 79.5% of patients had international normalized ratio correction (<1.5) within 1 hour of PCC therapy. Patients with intraparenchymal hemorrhage had an in-hospital mortality rate of 42.3% with median modified Rankin Scale of 5. Significant hematoma expansion occurred in 45.5%. There were 3 confirmed thrombotic complications within 7 days of PCC therapy. PCC therapy rapidly corrected international normalized ratio in the majority of patients, yet mortality and morbidity rates remained high. Rapid international normalized ratio correction alone may not be sufficient to alter prognosis after aaICH.

High Prevalence of Primary Antiretroviral Resistance Among HIV-1-Infected Adults and Children in Bahia, a Northeast State of Brazil

The introduction of highly active antiretroviral therapy (HAART) for treatment of AIDS patients significantly decreases AIDS-associated morbidity and mortality. Resistance to antiretroviral (ARV) drugs may arise during treatment however and is considered the main cause of therapy failure. Although the major reason for the onset of drug resistance is poor adherence to treatment acquisition of resistant strains of HIV also contributes to therapeutic failure. Brazil was the first country to provide free-of-cost ARV drugs for HIV therapy. This raised concerns about the selection and dissemination of drug-resistant HIV because of poor adherence and the extensive exposure to ARV drugs. Nevertheless the Brazilian AIDS program is considered a success and the available data show a low rate of HIV-1 drug resistance among drug-naive patients. To determine the prevalence of resistance mutations among recently diagnosed HIV-infected drug-naive patients we evaluated plasma or HIV-1 RNA samples stored at the Retrovirology Laboratory of the Federal University of Bahia Hospital in Salvador Brazil. The samples were obtained from the plasma of recently diagnosed patients from our AIDS clinics and from local blood banks after the year 2000. The institutional Ethics Review Board approved the study. (excerpt)

Histamine release from human buffy coat-derived mast cells

Mast cells are unique immune cells that release a spectrum of chemical mediators contributing to the inflammatory symptoms of allergic disorders. Although mast cell biology has been extensively studied in the rodents, research on human mast cells is hampered by the lack of a convenient preparation source. This problem has now been addressed by culturing human mast cells from CD34 + progenitors. We have recently discovered that human buffy coat preparations from local blood banks are an abundant and convenient source of progenitors for culturing mature mast cells which express functional high affinity IgE receptors and contain histamine and tryptase in their granules. In the current study, we further characterize these buffy coat-derived mast cells by studying their responses to common mast cell secretagogues and stabilizers. Mature human mast cells were obtained by culturing isolated progenitors in methylcellulose containing stem cell factor (SCF), IL-3 and IL-6 for 6 weeks and subsequently in liquid medium containing SCF and IL-6 for another 6 to 8 weeks. Following sensitisation with human IgE, these cells released histamine dose-dependently upon activation by anti-IgE and calcium ionophores while compound 48/80 and substance P were relatively ineffective. When the effects of anti-asthmatic agents on anti-IgE-induced mediator release from these cells were compared, only the β 2-adrenoceptor agonists and phosphodiesterase inhibitors produced dose-dependent inhibition but not cromolyn or nedocromil. In total, mast cells cultured from human buffy coat progenitors shared similar functional properties of MC T subtype of mast cells found predominantly in human lung parenchyma and intestinal mucosa.

A population‐based binational register for monitoring long‐term outcome and possible disease concordance among blood donors and recipients

Even with appropriate donor deferrals and advanced screening tests, the risk of disease transmission through blood transfusion cannot be completely disregarded. Efficient monitoring of possible disease transmission between blood donors and recipients should be an important component of a comprehensive haemovigilance system. We assembled the Scandinavian Donations and Transfusions (SCANDAT) database, with data on virtually all blood donors and recipients who have been registered at least once in any of the computerized local blood bank databases in Sweden and Denmark since the start of computerized registration in 1966. The records of these individuals, with their entire computerized donation and/or transfusion histories and all donor-component-recipient connections, were linked to nationwide population and health registers to attain essentially complete follow-up for up to 36 years regarding reproduction, hospital morbidity, cancer, and death. After data cleaning, the database contained 1,134,290 blood donors who contributed 15,091,280 records of donations and 1,311,079 recipients who received 11,693,844 transfusions. The data quality in the existing data sources was satisfactory. From the data obtained from local blood banks, 4.6%, 1.6%, and 6.4% of the person, donation, and transfusion records, respectively, had to be discarded after review of the legitimacy of recorded values, and comparisons with independent, external databases. It is possible to use existing computerized data, collected in routine health care, in haemovigilance systems for monitoring long-term outcome and disease concordance in blood donors and transfusion recipients.

Cord blood transplantation and cord blood bank in Japan.

The first cord blood transplantation (CBT) was performed in a 5-year-old boy with acute myelogenous leukemia from his HLA-identical sibling donor in 1994. Since then there have been 17 related and 131 unrelated CBTs in Japan. Overall survival and disease-free survival (DFS) were over 70% in sibling donor CBT. DFS of unrelated CBT in leukemia and other hematological malignancies was 43%, and OS of UCBT in non-malignant diseases was 63%. HLA disparity between the donor and the recipient did not affect the incidence and severity of acute graft-versus-host disease (GVHD) or survival. Cell dose was the most important factor for engraftment and survival both in malignant and in non-malignant diseases. The Japanese government has recently established the nationwide cord blood bank network, and eight local cord blood banks are financially supported by the government. 20,000 units of CB are planned to be collected and stored in the next 5 years by this network.

Tamizaje de enfermedades infecciosas en bancos de sangre, Colombia, 1995

As infectious diseases are the major cause of morbidity in Colombia, the objective of this study was to estimate the prevalence of blood transmitted diseases by screening tests in blood donors. Thus, the data collected from the National Blood-Bank Network as analysed. All records received during 1995 at the National Blood Banks' Coordination Centre from local blood banks al1 over the country were reviewed. Some variables such as: number of blood units obtained, number of blood units analysed and number of blood units reactive to HIV 112, hepatitis B and C viruses, human trophic lymphocyte virus, T pallidumand T cruziwere analysed. Epi lnfo 5.0. was used to analyse the data collected. 370,815 blood units were obtained from 172 blood banks. On average, the percentage of blood units analysed for markers such as HIV, hepatitis B and C viruses and T pallidum was higher than 99%; for other markers such as T cruziand HTLV 112 the percentages were 47% and 15%, respectively. Of the total blood units analysed, 0.3% were reactive to HIV-112, 1.2% to HTLV, 1 .O% to hepatitis C virus, 0.9% to hepatitis B virus, 1.2% to T cruzi and 1.4% to T. pallidum. These percentages correspond to national averages from reactive blood units analysed in all banks. Some blood banks reported values for anti-l: cruz; and HBsAg 4 times higher than the national average. The availability of blood units in this country is approximately 11 units per 1,000 inhabitants. 6.08% of screened blood units were reactive to any of the infectious agents described above. Blood screening was shown to be helpful in the prevention of infectious agents' transmission, at least, in the same number of infections per 100 possible transfusions. This analysis showed the need for better donor selection procedures at local blood banks in order to decrease infectious agents'transmission through blood transfusion.

Donating blood

The need for blood doesn't stop during the holidays, but unfortunately, many donations do. That's one reason why January has traditionally been National Volunteer Blood Donor Month—to remind people of the dwindling supply at their local blood banks and encourage them to take an hour of their time to help others. Anyone who is at least 17 years old and meets the weight minimum (about 110 pounds at most blood banks) can give, if health requirements are met. For example, you should not donate if you recently had a cold or flu, are taking antibiotics, or have ever had hepatitis or participated in high-risk sexual or injected-drug activities.

Evaluation of a pooling method for routine anti-HCV screening of blood donors to lower the cost burden on blood banks in countries under development.

A pooling system was developed for use in anti-HCV screening of voluntary blood donors at the local Central American Red Cross blood banks, in Nicaragua, El Salvador and Honduras. The commercially available second generation anti-HCV screening kit from Abbott Laboratories (North Chicago, IL) was used with a modification in the initial serum dilution procedure. Pools of five sera were selected for routine screening, based on comparative studies of individual samples and of pools with different sample sizes. During the years 1993 and 1994 a total of 89, 148 voluntary blood donors were screened and a positive prevalence rate of 0.35% was established. Of the initially positive samples, 54% confirmed positive, 30% were indeterminate and 16% were negative using the Abbott Matrix test. Significant differences of positive screening prevalence rates were found in the three countries, with average values of 0.50%, 0.23% and 0.08%, respectively, in Nicaragua, El Salvador and Honduras. These initially positive samples also showed a different confirmatory pattern with a positive rate of 64% in Nicaragua, in contrast to 20% in El Salvador. Only a few samples were available for RT-PCR amplification of HCV-RNA; however, this highly sensitive method did not appear to be more helpful than serology in confirming the HCV donor status. Overall, the data obtained indicate a fluctuation of HCV prevalence in voluntary blood donors among the three Central American countries. Further, differences were found in the percentages of initially screened positives and confirmation patterns. This information appears useful for establishing criteria in future screening policies. Thus, we suggest that the use of pooling for anti-HCV screening is beneficial in countries under development, since there are potential cost savings, as well as benefits in establishment of initial prevalence rates.

Issues in the Development of a Local Cord Blood Bank

An increasing number of transplants are being performed using stem cells harvested in placental and umbilical cord blood. Thus, interest in developing local cord blood banks is also increasing. This report discusses the issues and challenges that one may face in the development of a local cord blood bank.