ABSTRACTThe austenitic stainless steels are widely used in several engineering fields due to their high ductility, corrosion and high temperature performance. Despite its noble properties, components manufactured in austenitic stainless steel are subject to fatigue failure. Studies indicate that loading frequency can impact the austenitic stainless steel fatigue performance. In this scenario, the present study aims to evaluate the effect of frequencies of 3 and 30 Hz on the fatigue behavior of SS 304 alloy under load control in order to identify in which fatigue stage the effect is outstanding. Therefore, fatigue and fracture mechanics tests were evaluated on the alloy annealed at 1000°C. Furthermore, fatigue tests were also applied to the alloy after previous tensile plastic strain of 0.5. The analyses denoted a significant reduction in fatigue strength with increasing frequency, especially for the strained alloy. Fatigue crack nucleation is encouraged with greater load frequency. This behavior may be attributed to strain‐induced martensite and other strain mechanisms such as twinning and slip bands that are encouraged by lower strain rates but are relieved by auto‐heating achieved in higher frequencies, as mentioned in the literature, which decrease the strength to fatigue nucleation.