Transformer load losses cause various adverse effects, such as derating, a decreased lifetime, and greenhouse gas emissions. In this paper, the load losses caused by non-linear loads on distribution transformers are analyzed. For this study, the load loss expressions provided by the IEEE Standard C57.110 and ANSI/UL 1561-1562 were adapted to the usual case where the transformer currents differ in each phase. The novel load loss expressions adapted from the IEEE Standard C57.110 were applied using the software known as the “Transformer Loss Calculator” (TLC), implemented with LabVIEW. For the application of new load loss expressions, carbon dioxide (CO2) emissions were determined by multiplying the load losses by the emission factors of each country. The experimental results are based on the recordings made by a FLUKE 435 Series II analyzer on the second of two 1000 kVA transformers, feeding real residential distribution networks with very differently distorted loads. An analysis of these transformers shows that the annual energy losses and CO2 emissions obtained from the adapted load loss expressions could be more than 5% of those determined by the original IEEE and ANSI Standard expressions. Due to these percentage loss and emission differences, it is advisable to use the TLC software in transformer monitoring instruments.