Loading Capacity Research Articles

Design of 300nA quiescent current and 300mA load capacity LDO with fast transient response

Design of 300nA quiescent current and 300mA load capacity LDO with fast transient response

Synergistic effects of quercetin-loaded CoFe2O4@Liposomes regulate DNA damage and apoptosis in MCF-7 cancer cells: based on biophysical magnetic hyperthermia

Introduction Breast cancer (BC) is the most common malignancy in women globally. Significant progress has been made in developing structural nanoparticles (NPs) and formulations for targeted smart drug delivery (SDD) of pharmaceuticals, improving the precision of tumor cell targeting in therapy. Significance Magnetic hyperthermia (MHT) treatment using magneto-liposomes (MLs) has emerged as a promising adjuvant cancer therapy. Methods CoFe2O4 magnetic NPs (MNPs) were conjugated with nanoliposomes to form MLs, and the anticancer drug quercetin (Que) was loaded into MLs, forming Que-MLs composites for antitumor approach. The aim was to prepare Que-MLs for DD systems (DDS) under an alternating magnetic field (AMF), termed chemotherapy/hyperthermia (chemo-HT) techniques. The encapsulation efficiency (EE), drug loading capacity (DL), and drug release (DR) of Que and Que-MLs were evaluated. Results The results confirmed successful Que-loading on the surface of MLs, with an average diameter of 38 nm and efficient encapsulation into MLs (69%). In vitro, experimental results on MCF-7 breast cells using MHT showed high cytotoxic effects of novel Que-MLs on MCF-7 cells. Various analyses, including cytotoxicity, apoptosis, cell migration, western blotting, fluorescence imaging, and cell membrane internalization, were conducted. The Acridine Orange-ethidium bromide double fluorescence test identified 35% early and 55% late apoptosis resulting from Que-MLs under the chemo-HT group. TEM results indicated MCF-7 cell membrane internalization and digestion of Que-MLs, suggesting the presence of early endosome-like vesicles on the cytoplasmic periphery. Conclusions Que-MLs exhibited multi-modal chemo-HT effects, displaying high toxicity against MCF-7 BC cells and showing promise as a potent cytotoxic agent for BC chemotherapy.

Development of a tobramycin-loaded calcium alginate microsphere/chitosan composite sponge with antibacterial effects as a wound dressing

Traditional dressings exhibit several disadvantages, as they frequently lead to bacterial infections, cause severe tissue adhesion and perform a relatively single function. Therefore, in this study, a composite sponge dressing with antibacterial properties and excellent physicochemical properties was developed. Six groups of tobramycin-loaded calcium alginate microspheres were prepared by changing the amount of tobramycin added, and the optimal group was selected. Then, seven groups of tobramycin-loaded calcium alginate microsphere/chitosan composite sponges were fabricated via a solvent blending process and a freeze-drying method. The surface morphology, physicochemical properties, in vitro degradation properties, in vitro drug release properties, antibacterial properties and cytotoxicity of the composite sponges were examined. Group 3.0 contained the best microspheres with the largest drug loading capacity, good swelling performance and cumulative drug release rate, obvious and sustained antibacterial activity, and good cytocompatibility. The tobramycin-loaded calcium alginate microsphere/chitosan composite sponges exhibited three-dimensional porous structures, and their porosity, swelling rate, water absorption and water retention rates and water vapor transmission rate met the standards needed for an ideal dressing. The comprehensive performance of the sponge was best when 20 mg of drug-loaded microspheres was added (i.e. group 20). The cumulative drug release rate of the sponge was 29.67 ± 4.14% at 7 d, the diameters of the inhibition zones against the three bacteria were greater than 15 mm, and L929 cell proliferation was promoted. These results demonstrated that the tobramycin-loaded calcium alginate microsphere/chitosan composite sponge with 20 mg of tobramycin-loaded microspheres shows promise as a dressing for infected wounds.

Design of Polymeric Nanoparticles for Theranostic Delivery of Capsaicin as Anti-Cancer Drug and Fluorescent Nitrogen-Doped Graphene Quantum Dots.

In recent years, multifunctional nanocarriers that provide simultaneous drug delivery and imaging have attracted enormous attention, especially in cancer treatment. In this research, we designed a biocompatible fluorescent multifunctional nanocarrier for the co-delivery of capsaicin (CPS) and nitrogen-doped graphene quantum dots (N-GQDs) using the pH sensitive amphiphilic block copolymer (poly(2-ethyl-2-oxazoline)-b-poly(ε-caprolactone), PEtOx-b-PCL). The effects of the critical formulation parameters (the amount of copolymer, the concentration of poly(vinyl alcohol) (PVA) as a stabilizing agent in the inner aqueous phase, and volume of the inner phase) were evaluated to achieve optimal nanoparticle properties using Central Composite Design (CCD). The optimized nanoparticles demonstrated a desirable size distribution (167.8±1.4nm) with a negative surface charge (-19.9±0.4) and a suitable loading capacity for capsaicin (70.80±0.05%). The CPS & N-GQD NPs were found to have remarkable toxicity on human breast adenocarcinoma cell line (MCF-7). The solid fluorescent signal was acquired from cells containing multifunctional nanoparticles, according to the confocal microscope imaging results, confirming the significant cellular uptake. This research illustrates the enormous potential for cellular imaging and enhanced cancer therapy offered by multifunctional nanocarriers that combine drug substances with the novel fluorescent agents. This article is protected by copyright. All rights reserved.

Experimental study on the flexural performance of FRP grid-reinforced ultra-high-performance concrete I-beams with different steel fiber contents

To adapt to harsh engineering environments, the use of structural components with fiber-reinforced polymer (FRP) grid-reinforced ultra-high-performance concrete (UHPC) is considered a new development trend. Twelve FRP grid-reinforced UHPC I-beams are fabricated to investigate the effects of the thicknesses of FRP grids (0 mm, 1 mm, 3 mm, and 5 mm) and the steel fiber contents of UHPC (0%, 1%, and 2%) on their flexural performance. Four-point bending tests are conducted to obtain the failure modes, load-deflection characteristics, and strain responses of each testing beam, and a simplified calculation method for cracking loading and flexural bearing capacity is proposed. The results reveal that the increase in FRP grid thickness, the ultimate load bearing capacity of FRP grid-reinforced UHPC structural beams increases and the ductility also can be enhanced. The presence of steel fiber effectively inhibits the cracking and increases the first cracking load of the beam during the test process, although its influence on the ultimate bearing capacity is limited. Besides, until to the cracking stage, there is no noticeable change in the tensile strain of the FRP grids as the applied load increases. It has been used to simplify theoretical calculations, which shows good agreement with the experimental results.

Bond Properties of CFRP Externally Bonded Reinforcement on Groove in Concrete

Externally bonded reinforcement on groove (EBROG) is a significant reinforcement technology proposed by researchers to enhance the bond properties of reinforced concrete structural members. To understand the influence of groove size on concrete specimens of different strength, a total of 60 concrete specimens with 4 different strengths were cast with the single shear test in this paper, among which 48 EBROG specimens and 12 specimens with externally bonded reinforcement method (EBR) were used as the control group. The failure modes and failure mechanisms of specimens with various sizes and reinforcement methods were analyzed. Additionally, the test results of ultimate load, load–displacement curves, and bond-slip curves for specimens with different groove sizes were compared. The effectiveness of EBROG method in enhancing the ultimate load capacity at the bond interface of the specimens is proved. Furthermore, in situations where the volume of the groove was kept constant, the specimens with lower concrete strength and deeper groove exhibited superior bond properties. Also, the influence of groove width on bond properties was better than that of groove depth. Finally, the test results in this paper were compared with the prediction of the existing EBR and EBROG models to evaluate the performance of different models, and based on the original model, a prediction model for EBROG method in the groove region with higher accuracy was proposed.

Enhanced Tumor Site Accumulation and Therapeutic Efficacy of Extracellular Matrix-Drug Conjugates Targeting Tumor Cells.

The extracellular matrix (ECM) engages in regulatory interactions with cell surface receptors through its constituent proteins and polysaccharides. Therefore, nano-sized extracellular matrix conjugated with doxorubicin (DOX) is utilized to produce extracellular matrix-drug conjugates (ECM-DOX) tailored for targeted delivery to cancer cells. The ECM-DOX nanoparticles exhibit rod-like morphology, boasting a commendable drug loading capacity of 4.58%, coupled with acid-sensitive drug release characteristics. Notably, ECM-DOX nanoparticles enhance the uptake by tumor cells and possess the ability to penetrate endothelial cells and infiltrate tumor multicellular spheroids. Mechanistic insights reveal that the internalization of ECM-DOX nanoparticle is facilitated through clathrin-mediated endocytosis and macropinocytosis, intricately involving hyaluronic acid receptors and integrins. Pharmacokinetic assessments unveil a prolonged blood half-life of ECM-DOX nanoparticles at 3.65h, a substantial improvement over the 1.09h observed for free DOX. A sustained accumulation effect of ECM-DOX nanoparticles at tumor sites, with drug levels in tumor tissues surpassing those of free DOX by several-fold. The profound therapeutic impact of ECM-DOX nanoparticles is evident in their notable inhibition of tumor growth, extension of median survival time in animals, and significant reduction in DOX-induced cardiotoxicity. The ECM platform emerges as a promising carrier for avant-garde nanomedicines in the realm of cancer treatment.

Incorporating inherited variability into the drainage effect analysis of piezocone tests in gold tailings

The piezocone test (CPTu) is a commonly used field investigation method for analyzing the geomechanical behavior of mine tailings. However, the effect of drainage conditions on CPTu measurements is a critical factor in assessing tailings properties, particularly as tailings are often characterized as silty materials with intermediate permeability. Previous studies of drainage conditions have been hindered by the high variability of tailings materials, leading to considerable dispersion in experimental results. To address this challenge, the present paper proposes a numerical approach to characterize and incorporate the inherent variability of tailings to identify probabilistic limits of drainage. This approach involves characterizing site statistics through piezocone tests and incorporating this variability into a set of Monte Carlo analyses using cavity expansion theory. The results indicate that the probabilistic analysis accurately represents the variability of the normalized resistance, although there is a greater discrepancy when considering pore pressure measurements. As a practical application, probabilistic values of cone resistance were used to establish profiles of fully drained and undrained tests. These corrected profiles can then be used in load capacity methods, providing rational limits of behavior.

Multiaxial Fatigue Damage Analysis of Steel–Concrete Composite Beam Based on the Smith–Watson–Topper Parameter

The fatigue performance of steel–concrete composite beams is crucial for ensuring structural safety. To account for the member’s multiaxial stress state, this study employed the critical surface method, using fatigue damage parameters as an evaluation index for assessing fatigue performance. Static and fatigue performance tests on steel–concrete beams were conducted to identify failure characteristics, which informed the development of a finite element model that incorporates concrete damage. Using the SWT model, the most unfavorable loading parameters were determined by analyzing critical paths on the test beams, providing a basis for predicting how initial defects impact fatigue performance. The impact of initial defects on the fatigue performance of the composite beam is assessed using this criterion. The results indicate that the discrepancy between the actual and predicted load capacities of the test beam is within 5%, and cyclic loading significantly affects the test beam’s mechanical properties, resulting in a 27% reduction in load capacity and a 48% increase in deflection after 2 million cycles. Finite element modeling reveals that components experience multiaxial stress, with test beam mechanical property changes aligning with predicted fatigue damage parameters, confirming the reliability of using these parameters as a criterion. As the strength of the composite beams diminished due to pore defects, the fatigue damage parameter escalated, increasing the likelihood of crack formation. However, once the concrete’s strength fell to a level where the pegs were insufficiently constrained, the structural damage pattern shifted, and the fatigue damage parameter subsequently decreased.

Acid-Heat-Induced Fabrication of Nisin-Loaded Egg White Protein Nanoparticles: Enhanced Structural and Antibacterial Stability.

As a natural cationic peptide, Nisin is capable of widely inhibiting the growth of Gram-positive bacteria. However, it also has drawbacks such as its antimicrobial activity being susceptible to environmental factors. Nano-encapsulation can improve the defects of nisin in food applications. In this study, nisin-loaded egg white protein nanoparticles (AH-NEn) were prepared in fixed ultrasound-mediated under pH 3.0 and 90 °C. Compared with the controls, AH-NEn exhibited smaller particle size (112.5 ± 2.85 nm), smaller PDI (0.25 ± 0.01), larger Zeta potential (24 ± 1.18 mV), and higher encapsulation efficiency (91.82%) and loading capacity (45.91%). The turbidity and Fourier transform infrared spectroscopy (FTIR) results indicated that there are other non-covalent bonding interactions between the molecules of AH-NEn besides the electrostatic forces, which accounts for the fact that it is structurally more stable than the controls. In addition, by the results of fluorescence intensity, differential scanning calorimetry (DSC), and X-ray diffraction (XRD), it was shown that thermal induction could improve the solubility, heat resistance, and encapsulation of nisin in the samples. In terms of antimicrobial function, acid-heat induction did not recede the antimicrobial activity of nisin encapsulated in egg white protein (EWP). Compared with free nisin, the loss rate of bactericidal activity of AH-NEn was reduced by 75.0% and 14.0% following treatment with trypsin or a thermal treatment at 90 °C for 30 min, respectively.

Investigation on Seismic Performance of Reinforced Concrete Frame Retrofitted by Carbon Fiber-Reinforced Polymer

In order to improve the seismic performance of reinforced concrete (RC) frames, carbon fiber-reinforced polymer (CFRP) was used to retrofit reinforced concrete frame structures. The comparison pseudo-static test results show that the peak load, initial stiffness and ductility of the CFRP retrofitted model were increased by 43.89%, 39.27% and 30.1%, respectively. Based on the parametric study of the finite element model, the contribution of CFRP to the seismic upgrading effect of RC columns was quantitatively revealed, and an optimized design of retrofitted CFRP was proposed. The results show that the peak load, ductility and energy dissipation capacity of the whole structure are improved by using CFRP full-wrap reinforcement and strip reinforcement models with different coverage areas. The damage degree of the column decreases, the damage degree of the beam increases, and the failure mode changes from “column hinge” to “beam hinge”. Simultaneously, different CFRP reinforcement areas and the distance between strip CFRP have different reinforcement effects on concrete structures. Based on the investigation results, the recommended ratio of CFRP strip to spacing is 1 to 1.25.

A new modality of anti-cancer drug: Ferritin-drug conjugates.

e14590 Background: Success criteria of ADCs is the target overexpression in tumor, i.e. in general ~ 14-fold in tumor vs. normal cells, which is the reason why it is hardly possible to develop clinically potent ADCs against several cancers with low target expression, which remains a major challenge yet, although fam-trastuzumab deruxtecan-nxki (Enhertu; AZN) and sacituzumab govitecan-hziy (Trodelvy; Gilead) recently exhibit an exceptionally enhanced efficacy for TNBC. Methods: Payloads were conjugated to a human apoferritin-derived, cancer-targeting scaffold (a self-assembled 24-mer protein with a diameter of ~ 12 nm, showing a high avidity for cancer cells) via a particular payload-releasing linker based on thiol-maleimide/imidazole-metal ion/EDC-NHS chemistry. Results: A new modality of anti-cancer drug - ferritin-drug conjugates (FDCs) - was developed, demonstrating a notably enhanced performance in cancer treatment as compared to current, clinically available anti-cancer drugs such as fam-trastuzumab deruxtecan-nxki, which is due to the important advantages such as far lowered EC50, high cancer-targeting efficiency, excellent structural stability (even in DMSO (up to 30%)), high loading capacity (high drug to ferritin ratio (DFR > 20)) and consistent homogeneity in drug conjugation, no immune side effects, reduced drug resistance, and/or reduced off-target toxicity. Conclusions: FDCs are believed to open up a novel, attractive clinical route for both potent and safe therapy of a broad range of cancers.

Active Fabrics with Controllable Stiffness for Robotic Assistive Interfaces.

Assistive interfaces enable collaborative interactions between humans and robots. In contrast to traditional rigid devices, conformable fabrics with tunable mechanical properties have emerged as compelling alternatives. However, existing assistive fabrics actuated by fluidic or thermal stimuli struggle to adapt to complex body contours and are hindered by challenges such as large volumes after actuation and slow response rates. To overcome these limitations, we draw inspiration from biological protective organisms combining hard and soft phases, and propose active assistive fabrics consisting of architectured rigid tiles interconnected with flexible actuated fibers. Through programmable tessellation of target body shapes into architectured tiles and controlling their interactions by the actuated fibers, our active fabrics can rapidly (within seconds) transition between soft compliant configurations and rigid states conformable to the body (> 350 times stiffness change, loading capacity to weight ratio > 50) while minimizing the device volume after actuation. We demonstrate the versatility of our active fabrics as exosuits for tremor suppression and lifting assistance. We also present its potential as body armors for impact mitigation. Electrothermal actuators are integrated for smart actuation with convenient folding capabilities. Our work offers a practical framework for designing customizable active fabrics with shape adaptivity and controllable stiffness, these active fabrics have wide applications in wearable exosuits, haptic devices, and medical rehabilitation systems. This article is protected by copyright. All rights reserved.

Adaptive handover control parameters based on cell load capacity in a B5G/6G heterogeneous network

Adaptive handover control parameters based on cell load capacity in a B5G/6G heterogeneous network

Spherical Assembly of Halloysite Clay Nanotubes as a General Reservoir of Hydrophobic Pesticides for pH-Responsive Management of Pests and Weeds.

The development of smart systems for pesticidal delivery presents a significant advancement in enhancing the utilization efficiency of pesticides and mitigating environmental risks. Here an acid-responsive pesticidal delivery system using microspheres formed by the self-assembly of halloysite clay nanotubes (HNTs) is proposed. Insecticide avermectin (AVM) and herbicide prometryn (PMT) are used as two models of hydrophobic pesticide and encapsulated within the porous microspheres, followed by a coating of tannic acid/iron (TA/FeIII) complex films to generate two controlled-release pesticides, named as HCEAT and HCEPT, resulting in the loading capacity of AVM and PMT being 113.3 and 120.3mgg-1, respectively. Both HCEAT and HCEPT exhibit responsiveness to weak acid, achieving 24h-release ratios of 85.8% and 80.5% at a pH of 5.5. The experiment and simulation results indicate that the coordination interaction between EDTA2- and Ca2+ facilitates the spherical aggregation of HNTs. Furthermore, these novel pesticide formulations demonstrate better resistance against ultraviolet (UV) irradiation, higher foliar affinity, and less leaching effect, with negligible impact of the carrier material on plants and terrestrial organisms. This work presents a promising approach toward the development of efficient and eco-friendly pesticide formulations, greatly contributing to the sustainable advancement of agriculture.

Improved Tribological Performance of ta-C/MoSx Coatings Deposited on Laser Micro-Structured Steel Substrates in Both Vacuum and Air

Vacuum and air atmospheres impose very different requirements on tribological-loaded contacts, which usually require different surface materials. While hard tetrahedral amorphous carbon (ta-C) coatings provide good tribological properties in air, soft coatings such as molybdenum disulfide (MoS2) work well in a vacuum. Tribological performance in the respective other environment, however, is poor. In this work, the combination of laser microstructured (direct laser interference patterning) steel substrates and the deposition of ta-C and MoSx coatings with vacuum arc evaporation (LaserArc™) was studied, resulting in steel/DLIP, steel/DLIP/ta-C, steel/DLIP/MoSx, steel/DLIP/ta-C/MoSx, and steel/MoSx surface combinations. The tribological properties were studied using a ball-on-disk tribometer with a steel ball counter body in air and in a vacuum (p < 5 × 10−7 mbar). The type of the topmost coating governed their tribological properties in the respective atmosphere, and no general beneficial influence of the microstructure was found. However, steel/DLIP/ta-C/MoSx performed best in both conditions and endured the highest contact pressure, which is attributed to the mechanical support of the ta-C coating and MoSx reservoir in the remaining structure, as evidenced by Raman spectroscopy. The findings suggest that such combination allows for surfaces bearing a high load capacity that can be applied in both a vacuum and in air, for example, in multi-use space applications.

Reversible Fusion-Fission MXene Fiber-Based Microelectrodes for Target-Specific Gram-Positive and Gram-Negative Bacterium Discrimination.

Inaccurate or cumbersome clinical pathogen diagnosis between Gram-positive bacteria (G+) and Gram-negative (G-) bacteria lead to delayed clinical therapeutic interventions. Microelectrode-based electrochemical sensors exhibit the significant advantages of rapid response and minimal sample consumption, but the loading capacity and discrimination precision are weak. Herein, we develop reversible fusion-fission MXene-based fiber microelectrodes for G+/G- bacteria analysis. During the fissuring process, the spatial utilization, loading capacity, sensitivity, and selectivity of microelectrodes were maximized, and polymyxin B and vancomycin were assembled for G+/G- identification. The surface-tension-driven reversible fusion facilitated its reusability. A deep learning model was further applied for the electrochemical impedance spectroscopy (EIS) identification in diverse ratio concentrations of G+ and G- of (1:100-100:1) with higher accuracy (>93%) and gave predictable detection results for unknown samples. Meanwhile, the as-proposed sensing platform reached higher sensitivity toward E. coli (24.3 CFU/mL) and S. aureus (37.2 CFU/mL) in 20 min. The as-proposed platform provides valuable insights for bacterium discrimination and quantification.

E-Bicycle for Food Delivery

Abstract: The rise of urban food delivery services has led to an increased demand for efficient and environmentally sustainable transportation methods. This study explores the implementation of sustainable electric bicycles (e-bikes) for food delivery, examining their potential to reduce carbon emissions, traffic congestion, and operational costs. E-bikes, powered by rechargeable batteries, offer a viable alternative to traditional delivery vehicles, particularly in densely populated urban areas. Key factors influencing the adoption of e-bikes include battery life, charging infrastructure, load capacity, and regulatory support. The research also highlights successful case studies from various cities, demonstrating the environmental and economic benefits of integrating e-bikes into delivery fleets. The findings suggest that with proper investment and supportive policies, ebikes can significantly contribute to more sustainable urban logistics, promoting a greener and more efficient food delivery ecosystem.

Developing a Community-Based Exercise and Physical Programme for Runners With Patellofemoral Pain in Under-Resourced Communities: A Delphi Study

Background: Patellofemoral pain (PFP) is a multifactorial condition that is highly prevalent among recreational runners. Poor homeostasis between load and runner's capacity is the main cause of PFP. A low-cost and community-based intervention is needed to address the rehabilitation needs of runners in under-resourced communities. Purpose: To develop a community-based exercise and physical programme for runners with PFP in under-resourced communities. Method: This study used a Delphi method that included sports experts (physicians, physiotherapists, biokineticists, podiatrists, and sports therapists) who consented to participate. Delphi included three rounds of iterations to attain consensus among experts. Experts reached a consensus by rating PFP programme items using the 5-point Likert scale. Results: Ten experts participated. There were more males ( n = 6) than females ( n = 4). Participants were aged between 36 and 55 years ( n = 5) and had 16-20 years of clinical experience ( n = 5). Delphi process yielded an exercise and physical intervention, which included the following 10 recommended strategies: hip muscle training, quadriceps muscle training, general lower limb and trunk strengthening, use of appropriate running shoes, and use of prefabricated in-shoe foot orthosis, tissue mobilisation, patellar taping/bracing, education, flexibility exercises, addressing external loads, and addressing nonphysical internal loads. Conclusions: A consensus was reached for a suitable exercise and physical programme for runners in under-resourced communities. A follow-up implementation study is, therefore, recommended.

Bioaugmented biological contact oxidation reactor for treating simulated textile dyeing wastewater

In this study, modified polyamide fibers were used as biocarriers to enrich dense biofilms in a multi-stage biological contact oxidation reactor (MBCOR) in which partitioned wastewater treatment zone (WTZ) and bioaugmentation zone (BAZ) were established to enhance the removal of methyl orange (MO) and its metabolites while minimizing sludge yields. WTZ exhibited high biomass loading capacity (5.75 ± 0.31 g/g filler), achieving MO removal rate ranging from 68 % to 86 % under different aeration condition within 8 h in which the most dominant genus Chlorobium played an important role. In the BAZ, Pseudoxanthomonas was the dominant genus while carbon starvation stimulated the enrichment of chemoheterotrophy and aerobic_chemoheterotrophy genes thereby enhanced the microbial utilization of cell-released substrates, MO as well as its metabolic intermediates. These results revealed the mechanism bioaugmentation on MBCOR in effectively eliminating both MO and its metabolites.