LLLI Research Articles

Protective effect of low level laser irradiation on liver ischemia-reperfusion injury in rats

Objective To investigate the protective effect of low level laser irradiation on liver ischemia-reperfusion injury (HIRI) in rats. Methods Forty-five health male Sprague-Dawley rats were randomly divided into three groups: Sham group, ischemia-reperfusion group (Control group) and 1ow level laser irradiation group (LLLI group). After the hepatic inflow was occluded for 30 min and reperfused for 90 min, serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were determined, and the liver specimen were obtained for the determination of superoxide dismutase (SOD) activities and malondialdehyde (MDA) contents and for microscopic examinations. Results As compared with the Sham group, the levels of ALT [(577.98±134.65) U/L, (233.11±108.23) U/L], AST [(1 213±314.8) U/L, (563.32±289.77) U/L], LDH [(2 985.96±478.88) U/L, (1 086.24±1 068.31) U/L], MDA [(106.87±30.34) U/L, (54.23±20.37) U/L)] in Control group and LLLI group was significantly increased (P<0.05), and the activity of SOD [(33.15±3.51) U/L, (59.11±7.01) U/L] was significantly lower than in Sham group (P<0.05). As compared with the Control group, the levels of ALT, AST, LDH and MDA in LLLI group were significantly reduced (P<0.05), and the activity of SOD was significantly elevated (P<0.05). Conclusion LLLI has a protective effect on rat livers against ischemia-reperfusion injury, which is related with antioxidation. Key words: Low level laser irradiation; Liver ischemia; Reperfusion injury; Superoxide dismutase; Malondialdehyde

Study on the effect of low level laser in the treatment of periodontal disease

Objective The purpose of this study was to explore the efficacy of low level laser treatment (LLLT) combined with initial periodontal therapy in treating chronic periodontits.Methods Eighteen patients with chronic periodontitis were randomly treated in a spit-mouth design with combination of laser (808 nm,80 mW) treatment with scaling and root planing (experimental) or with scaling and root planing alone (contol) after OHI and supragingival cleaning.Clinical parameters of sulcus bleeding index (SBI),periodontal probing depth (PD) and clinical attachment loss (CAL) of both sides were recorded and gingival crevicular fluid samples were collected at baseline and 1,4,12 weeks after the treatment.GCF samples were analyzed for b-FGF,IL-1β,IL-8 level using ELISA assay.Results For the change of SBI and PD,only SBI in the experimental group experienced significant reduction (P＜0.05) at 1 week compared with baseline (P＜0.05),and the reduction in both groups were statistically significant (P＜0.05) at 4 weeks.The same situation occurred while both experimental groups made more significant difference (P＜0.01) at 12 weeks.The reduction of SBI was obvious in both groups when the PD was more than 6 mm.For CAL,the experimental group showed signs of improvement (P＜0.05) at 4 weeks; both groups indicated improvement (P＜0.05),while the experimental was more significant (P＜0.01) at 12 weeks.For the levels of b-FGF,it showed steady rise after treatment in experimental group,while it went up to the baseline after falling in control group.At 12 weeks,there were statistical significance of differences between two groups (P＜0.05).The changes of IL-1β and IL-8,in the experimental group showed better results than that in control group,while the difference between two groups was not significant (P＞0.05).Conclusion LLLI can accelerate the healing of periodontal tissue by reducing the SBI,decreasing the PD,improving the CAL and increasing levels of b-FGF in the GCF of the patients. Key words: Low-level lasers; Periodontitis; b-FGF; Cytokines

ILCA, IBLCE, and LLLI: distinct missions define breastfeeding organizations.

ILCA, IBLCE, and LLLI: distinct missions define breastfeeding organizations.

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