Live Weight Gain Research Articles

Liver knockout of MCU leads to greater dysregulation of lipid metabolism in MAFLD.

Metabolic-associated fatty liver disease (MAFLD) is a common chronic condition that poses a significant threat to human health. Mitochondrial dysfunction, particularly involving the mitochondrial Ca2+ uniporter (MCU), plays a key role in its pathogenesis. This study aimed to investigate the impact of the MCU gene on hepatic lipid metabolism in mice fed a high-fat diet. Using MCU knockout and wild-type mice, subjected to either a high-fat or normal diet for 14weeks, we observed notable Steatosis and liver weight gain in MCU-deficient mice. Liver function markers, serum triglycerides, very low-density lipoprotein (VLDL) levels, and ApoB protein expression were all significantly elevated. Mechanistic studies revealed that MCU deletion led to mitochondrial dysfunction, increased oxidative stress. These findings highlight the critical role of the MCU gene in maintaining hepatic lipid balance and suggest its potential as a therapeutic target for managing nonalcoholic fatty liver disease.

Sequential fermentation of Ginkgo biloba seeds by Bacillus subtilis natto and Lactobacillus plantarum enhanced nutrition, flavor and lipid-lowering activity.

Ginkgo biloba seeds (GBS) are rich in flavonoids, proteins and reducing sugar, and have been consumed as food and medicinal nuts for thousands of years. However, the presence of ginkgotoxins and their poor palatability limit people's consumption of them. This study used solid-state fermentation with Bacillus subtilis natto and Lactobacillus plantarum to enhance the safety and benefits of GBS. Optimized fermentation conditions increased the content of beneficial components like total flavonoids, soluble protein and reducing sugar while eliminating unpleasant odors (isoamyl aldehyde and hexanal) and reducing the toxin 4'-O-methylpyridoxine by 91.17%. Fermentation of GBS powder can significantly enhance its anti-inflammatory and antioxidant activities in vitro (P < 0.001). Furthermore, it exhibits a dose-dependent effect within a certain concentration range. Mixed fermentation (FBnLp) was evaluated for its effects on obesity and metabolic syndrome in mice fed a high-fat diet. FBnLp significantly reduced body and liver weight gain, prevented dyslipidemia and decreased inflammatory and oxidative stress compared to unfermented GBS. Histological analysis showed that FBnLp improved liver health by reducing fat accumulation and preventing non-alcoholic fatty liver disease. Meanwhile, it was found that feeding FBnLp increased the expression of CPT-1α, which regulates energy expenditure and fat breakdown, and downregulated the expression of SREBP-1c, FAS and ACC, which regulate fat synthesis. This research provides new insights and technological support for the application and development of FBnLp as a functional product, addressing key issues in its use and industry growth. © 2024 Society of Chemical Industry.

Factors influencing the variability in performance of cattle grazing tropical pasture

Context Whereas it is known that there is variation in liveweight gain (described as performance in this paper) within a group of grazing cattle, the causes of this variation are not well understood. Aim A 2-year grazing study in northern Queensland was conducted to understand the relative influence of diet selection, grazing behaviour and rumen fermentation on variation in performance of growing cattle. Methods Eighty-nine Bos taurus × B. indicus (~6 months old) growing steers were grazed as a group on mixed tropical pasture for 23 months. Pasture was characterised for biomass, species and nutrient composition. Cattle were ranked according to overall liveweight (LW) gain and the top (high performance, HP) 20 and bottom (low performance, LP) 20 head were compared for diet composition, rumen fermentation and grazing behaviours. Results Pasture biomass, and nutritive value of pasture and diet varied between seasons and years of study. The HP cattle achieved overall LW gains 20% greater than those of the LP cattle mainly due to higher rates of gain in the wet season. However, the nutritive value and rumen fermentation characteristics were similar for both LP and HP cattle, although there was evidence that crude protein and digestibility were modestly higher in diets of HP cattle, especially in the first wet season. Activity, measured as distance travelled, declined as pasture biomass declined, and cattle appeared to favour pasture with higher legume content. Conclusion It is concluded that divergence in performance of cattle on tropical pasture could not be fully explained by measurements taken in this study. Diet selection for plant components with improved nutritive value probably played a small role. Differences in initial LW between the LP and HP groups may have been a contributory factor. Implications Despite comprehensive measurements of factors that influence efficiency and performance on pasture, we were unable to identify definitive causes. Genetic variation in feed intake or efficiency of digestion needs to be investigated.

Effect of Supplementation of Spirulina (Spirulina platensis) on Growth Performance of Broiler in Konkan Region, India

The experimental trial of six weeks was conducted on 200 broiler chicks. They were randomly distributed into five experimental treatment groups. Each treatment group was replicated into four with 10 birds per replication. The control (T1) group was fed standard ration and T2, T3, T4 and T5 group were provided same standard ration supplemented with 1.00 g, 2.00 g, 3.00 g and 4.00 g spirulina powder, respectively. The result of experimental trial showed that supplementation of spirulina powder was significantly highest live body weight in treatment T5 (1944.50 g/bird) as well as average live body weight gain (277.79 g/bird). It is concluded that, 4.00 g of spirulina powder significantly improved growth performance of broiler chicks than other treatments groups.

Effect of Feed Supplementation of Shatavari Root Powder (Asparagus racemosus) on Growth Performance of Broiler in Konkan Region

The present study aimed to highlights the effect of feed supplementation of Shatavari root powder (Asparagus racemosus) on growth performance of broiler in Konkan region. A substantial portion of the production expenses is attributed to the cost of feed and in a developing country like India, the supply of quality and affordable feed has been inconsistent. To enhance the overall productivity of poultry birds, it is imperative to maintain a reliable source of low-cost feed. The experimental trial of six weeks was undertaken for total 200-day old broiler chicks. They were randomly distributed into five experimental diets. Each treatment group was replicated into four with 10 birds per replicate. The control (T0) group was fed standard ration and T1, T2, T3, and T4 group were provided same standard ration supplemented with 5.00, 10.00 , 15.00 and 20.00 g /kg Shatavari root powder, respectively. Result of experiment showed that supplementation of Shatavari root powder was significantly highest live body weight in treatment T2 (1852.50 g/bird) as well as average live weight gain (264.64 g/bird). It is concluded that, 10.00 g of Shatavari root powder significantly improved growth performance than other treatments groups.

Effects of a synthetic analogue of the bovine appeasing pheromone on the overall welfare of dairy calves from birth through weaning

Environmental enrichment in the form of synthetic analogs of appeasing pheromones have shown promising results in improving the welfare of domestic animals including dogs, pigs, horses, and cattle. The main objective of this study was to determine if the use of the bovine appeasing pheromone (BAP) would improve the welfare of dairy calves; therefore, in this randomized controlled trial, 72 Holstein Friesian dairy calves were housed in individual hutches after birth and were randomly allocated to receive BAP or a placebo once every 2 weeks from birth through weaning. After weaning, calves were moved to group hutches according to treatment for 4 additional weeks. It was hypothesized that dairy calves treated with BAP would display fewer signs of stress compared with calves receiving the placebo during the weaning process. To operationalize stress, calves were fitted with triaxial accelerometers on the hind leg after birth, and activity levels were monitored throughout the experiment. Data on live weight gain (ADG) and cortisol levels in saliva and hair were also obtained. Calves were fitted with heart rate monitors every week for at least 24 h to assess heart rate variability (HRV). The use of BAP had a positive effect on ADG after weaning and during group housing and resulted in increased resting time after weaning. Moreover, BAP, was associated with a reduction in the activation of the neuroendocrine system evidenced by higher HRV parameters after weaning, including increased standard deviation of beat to beat of normal sinus beats (SDNN) and root mean squares of successive differences (RMSSD). These results suggest a potential welfare benefit of the use of BAP during the artificial rearing of dairy calves.

The impact of enhanced early postnatal nutrition on the histomorphological features of the ovaries in fat-tailed Sanjabi ewe lambs.

This study aimed to investigate whether providing additional ad libitum feeding could improve reproductive characteristics in prepubertal ewe lambs. Twelve Sanjabi ewe lambs, approximately one-month-old and with an average initial weight of 11.6 ± 1.3kg, were monitored over a 3-month period, which was divided into three distinct periods of 30 days each. The first period spanned from the lambs' age of one to two months, the second period covered their age range of two to three months, and the third period encompassed their age range of three to four months. The lambs were randomly assigned to either a control group (C) or a treatment group (T). The control group received a basal diet consisting of hay and milk, whereas the treatment group received an enhanced diet comprising hay, milk, and concentrate. Live weight (LW) and average daily gain (ADG) were measured weekly, and blood samples were collected from the jugular vein at days 30, 60, and 90 of the experiment to determine the concentrations of progesterone (P4) and estrogen (E2). Following the 90-day experiment, the lambs were humanely euthanized in accordance with established protocols. Subsequently, both ovaries from each animal were removed, weighed and subjected to a comprehensive histological analysis. The ovaries underwent fixation, embedding, and sectioning procedures, followed by staining with hematoxylin and eosin (H&E) to facilitate microscopic examination. Ovary microscopic sections from both C and T groups were examined to assess follicles at various developmental stages, including primordial follicle number (PreFN) and diameter (PriFD), primary follicle number (PriFN) and diameter (PriFD), secondary follicle number (SFN) and diameter (SFD), antral follicle number (AFN) and diameter (AFD), and granulosa layer thickness of secondary (GLTSF) and antral follicles (GLTAF). The impact of diet and three experimental periods on ADG and LW exhibited significant variations. There was a significant increase (P < 0.05) in LW in the T group compared to the T group, with noticeable differences in LW fluctuations observed during distinct periods. However, ADG was more prominent in the initial and second observation periods than in the third. The impact of diet and experimental periods on P4 and E2 concentrations was found to be negligible. The T group showed significant improvements (P < 0.05) in PreFD, PriFN, and GLTAF compared to the C group. This study suggests that ad libitum concentrate feeding may promote reproductive characteristics in prepubertal ewe lambs. Further research is needed to explore its potential impact on the age of puberty.

Enhancing Broiler Growth and Carcass Quality: Impact of Diets Enriched with Moringa oleifera Leaf Powder Conjugated with Zinc Nanoparticles

This study evaluated the effects of Moringa oleifera leaf powder extract stabilized with zinc nanoparticles (ZnNPs-MLPE) as a natural growth promoter in broiler diets. Randomly assigned 264 one-day-old Ross 308 chicks to four different feeding treatments, with each group being subdivided into six replicates, each comprising 11 unsexed chicks. The control group was fed a basic diet without additives, while the experimental groups were supplemented with 1.0, 2.0, or 3.0 cm³ of ZnNPs-MLPE/L of diet. The findings demonstrated that 2.0 and 3.0 cm³/L ZnNPs-MLPE supplementation significantly enhanced live body weight (LBW) and weight gain (BWG). Feed intake (FI) and feed conversion ratio (FCR) did not show significant differences between the treated groups and the control, indicating that the additive did not negatively affect feed efficiency. However, an increase in abdominal fat was noted in the ZnNPs-MLPE treatments relative to the control. Blood analysis revealed that the ZnNPs-MLPE groups had significantly lower levels of "total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL), very low-density lipoprotein (VLDL), and alanine transaminase (ALT) compared to the control group. In contrast, total protein (TP), albumin, and the albumin/globulin (A/G) ratio" were higher in the ZnNPs-MLPE groups. Immunoglobulins IgY and IgM, as well as superoxide dismutase (SOD) levels, were elevated. Malondialdehyde (MDA) levels were reduced, indicating improved antioxidant capacity and immune function in the ZnNPs-MLPE-treated groups. In conclusion, supplementation with ZnNPs-MLPE at 2.0 and 3.0 cm³/L positively impacted broiler growth efficiency, antioxidant capacity, and immunological functionality. These findings support the potential of ZnNPs-MLPE as an effective natural growth enhancer for producing healthier poultry products.

Copper requirements of animals and pastures in New Zealand pastoral agriculture - a review

More copper (Cu) is required for the growth of cattle and deer than sheep and pasture. National and regional farm surveys showed that mean pasture Cu concentrations were generally within or above the required range for pasture growth and lambs but below that for cattle and deer. Pasture concentrations of molybdenum (Mo) of up to 7 mg/kg for lambs and 10 mg/kg for deer had no effect on liveweight gain provided that pasture Cu concentrations were 7 to 8 mg/kg or greater. From twenty-one sites, there was a significant response to Cu in animal liveweight gain and wool weight only from lambs injected monthly with Cu and deer grazing pasture top dressed with copper sulphur, both on pasture with high Mo concentrations. Daily drenching of animals with copper sulphate or chelated Cu and the use of Cu capsules or copper oxide (CuO) wire particles was more effective at increasing liver Cu concentration than Cu injections or topdressing with copper sulphate. There was a significant pasture yield response measured on Recent and podzolised soils that had pasture Cu concentrations in the deficient range. There is a need for more research on the response in liveweight gain of cattle and deer from different Cu treatments at varying pasture Mo concentrations.

Impacts of winter crop choice and ewe body condition score change on whole farm profitability and production

Variations in body condition score (BCS) in ewes influences lamb survival, ewe survival and ewe lactation performance. Feeding a low protein diet such as fodder beet to ewes in mid-to-late pregnancy increases lamb losses and reduces lamb weaning weight even further. However, using high yielding forages such as fodder beet can reduce the area of winter crop required, introducing potential whole-farm benefits which may alter profitability. We analysed the effects of winter crop choice (brassica or fodder beet) and BCS (maintaining or reducing) on whole-farm production and profitability, using a targeted supplement to offset the impacts of fodder beet feeding. Eight farms were selected to represent low or high rainfall (590 and 1090 mm/annum respectively) regions of the South Island. Impacts of changing BCS on ewe and lamb survival at lambing, and lamb liveweight gain to weaning were calculated when fed either adequate (brassica) or low protein (fodder beet) diets in mid-to-late pregnancy. Production statistics and profitability were calculated using Farmax. Whole farm profitability was analysed using BCS change and winter forage crop as main treatments, blocked by low or high rainfall. Applying the effects of BCS and low protein diets on reproductive performance from prior published studies resulted in variation of 102 to 141 lambs weaned per 100 ewes mated, with the lowest value when the low protein, fodder beet only diet was used. Greatest number of lambs weaned was achieved when BCS was maintained through winter through provision of a balanced supplement, regardless of winter forage diet (P&lt;0.05). Lamb sale dates were later when fodder beet was used, due to lower lamb weaning weights, resulting in marginally higher prices due to changes in pricing schedule. Farm profitability was increased by 25% when BCS was maintained though winter (P&lt;0.05) irrespective of the diet used. Use of fodder beet must be accompanied by targeted supplementation to avoid ewe BCS loss increased lamb losses and the negative effects on wastage and animal welfare. Extra profitability could only be generated by opportunities to sell surplus feed during the summer. These responses were similar in both high and low rainfall environments. This study is unique in testing measured responses of BCS and targeted nutritional supplementation under specific physiological conditions on whole-farm profitability. Farmer decisions on feed utilisation and sale also have a significant influence on the potential to mitigate the effects of variations in winter forage choice.

Effect of ethanol extract from Enterolobium cyclocarpum fruit on Leghorn chickens exposed to Eimeria.

There are concerns about residues of drugs in meat that are used to prevent coccidiosis in chickens. Natural compounds are an alternative to drugs. Two studies investigated the effect of an extract of Enterolobium cyclocarpum fruits (EEC) in the feed of male Leghorn chickens exposed to Eimeria spp. In the first experiment, the administration of EEC after infection with Eimeria spp. was investigated over 16 days. One thousand chickens were randomly housed in 20 pens of 1 m2 each. The pens were randomly assigned to each treatment. Five treatments were administered, containing 150, 300 and 450mg/kg of EEC in the feed, the fourth treatment (C) contained 0.5g/kg of a commercial anticoccidial, and the fifth treatment provided no treatment (WA). The second experiment lasted 18 days. Administration of the EEC began five days before the chickens were infected with Eimeria spp. Four hundred and eighty chickens were randomly allocated to 24 pens of 1 m2. The pens were randomly assigned to each treatment. In the second experiment, the same five treatments were tested and one additional treatment containing 300mg EEC plus 1g of polyethylene glycol (PEG)/kg of feed (E300PEG). In the experiment one chickens in the EEC treatments had lower faecal oocyst excretion (OE) on day 14 post infection with Eimeria spp., than chickens in the WA treatment (P < 0.05). A reduction in live weight gain (LWG) was observed in the EEC treatments (P < 0.05). In the second experiment, the excretion of oocysts in chickens from the EEC and E300PEG treatments on day 13 post-infection with Eimeria spp. was the same as in the C treatment and lower than in the WA treatment (P < 0.05). LWG was lower in the EEC treatments than in the C treatment (P < 0.05). However, the Chickens in the E300PEG and C treatments had similar LWG (P > 0.05) suggesting that PEG inhibits the negative effect of EEC tannins on LWG. In conclusion, the addition of EEC to chicken feed reduced both OE and LWG. Treatment with EEC and PEG (E300PEG) reduced the excretion of oocysts without negative effects on LWG.

Evaluate the role of Moringa oleifera in improving the performance and productivity of broiler chickens

Moringa oleifera, popularly known as the "tree of life" or "miracle tree," is considered an essential herbal plant due to its numerous medical and non-medicinal properties. So, the current study aimed to evaluate the role of M. oleifera in improving the performance and productivity of broiler chickens. 120 broiler chicks of the Lohman breed were raised in the poultry field belonging to the College of Agriculture/University of Tikrit for the period from February to April 2024. The herd was divided into three groups: the first group was the control treatment, and the second group was given 10% M. oleifera leaf powder to the diet. As for the third group, 10% M. oleifera seed oil was used with the feed, this study was designed to calculate the following criteria: live body weight, weight gain, feed intake, and mortality rate. The results showed that the live body weight was better in treatment with M. oleifera seed oil (2095.16±25.42) compared to treatment with M. oleifera leaves (1983.14±29.26) still, when compared to the control group (1842.65±13.57), the two groups had a significant difference (P≤0.05). The average weight gain was better in treatment with M. oleifera seed oil (1968.83±12.62) compared to treatment with M. oleifera leaves (1935.37±8.02), but the two groups showed a significant difference (P≤0.05) compared with the control group (1820.61± 11.35). The average of feed intake was better in treatment with M. oleifera seed oil (3391.41±9.57) compared to treatment with M. oleifera leaves (3315.27±11.82) still, when compared to the control group (3137.18±14.21), the two groups had a significant difference (P≤0.05). the mortality rate was better in treatment with M. oleifera seed oil (2.5%) compared to treatment with M. oleifera leaves (5.0%), but the two groups were better than the control group (7.5%). it is concluded that the leaves and seed oil of M. oleifera are a good enhancer in improving the performance and productivity of broiler chickens.

The impact of LEP gene polymorphisms located at exon 2 (LEP-HinfI) and intron 2 (LEP-Sau3AI) on growth and reproductive traits in Saanen goats

Abstract. Leptin (LEP), alternatively recognized as the obesity gene, influences food consumption, energy balance, and lipid metabolism. Additionally, it plays a crucial role in energy metabolism, and variations in the LEP gene have been studied extensively among various livestock species. These investigations have unveiled correlations with traits such as meat quality, milk yield and composition, and growth characteristics. Nevertheless, the existing knowledge regarding its correlation with growth and reproductive traits in goats is comparatively limited, particularly when juxtaposed with studies of cattle. Hence, the objective of the current study was to investigate the relationship between polymorphisms in the intron 2 (Sau3AI) and exon 2 (HinfI) regions of the LEP gene and growth and reproductive traits in Saanen goats. The study comprised 185 Saanen goats in total. The PCR-RFLP (polymerase chain reaction restriction fragment length polymorphism) technique was employed to genotype these polymorphisms. Population genetic analysis included the number of effective alleles, heterozygosity, polymorphism information content, and compatibility with the Hardy–Weinberg equilibrium. The general linear model procedure's least-squares methods were used for the statistical analysis. The SNP (single-nucleotide polymorphism) located in exon 2 (LEP-HinfI) was monomorphic. Remarkably, the SNP located in intron 2 (LEP-Sau3AI) was associated with birth weight (p&lt;0.05) and average daily weight gain (p&lt;0.05). In this context, the AA genotype demonstrated higher birth weight and daily live weight gain means compared to other genotypes. No significant differences were observed in litter size, total weight gain, or morphometric measurements. The caprine LEP-Sau3AI polymorphism situated within intron 2 proved influential in traits critical for the profitability and sustainability of goat breeding. The findings of this study provide novel and valuable information for future research on the LEP gene in goats.

Statistical Models and Evaluation Criteria Used in Poultry Farming

In this thesis study, the statistical models and evaluation criteria used in poultry breeding were examined comprehensively, taking into account live weight gain, egg weight and egg numbers. For this purpose, as an example of live weight increase; 9-week live weight gains of Ross-308 broiler line, as an example of egg weight; The average of 20-week-old egg weights of brown Lohmann laying hens and 9-week egg yields of Japanese quail were used as an example of the number of eggs. In modeling, for live weight gain; 10 different models for egg production; 11 different models and 8 different models for egg weight were considered. In evaluating the models; error mean squares, coefficient of determination, corrected coefficient of determination, Akaike information criterion, corrected Akaike information criterion, Bayesian information criterion, accuracy factor, deviation factor and Durbin-Watson autocorrelation values were taken into consideration. As a result of the study, in terms of live weight gain; The cubic piecewise regression model is the best model in terms of egg yields; It was determined that the modified Compartmental model and the logistic model gave better results than the others in terms of egg weight. The worst models are in live weight gains; Brody, egg yields; It was concluded that there was a quadratic linear and Von Bertalanffy model for egg weight.

Influence of different doses of essential amino acids on growth and development of broiler chickens

Optimization of the amino acid balance of essential amino acids, as well as individual amino acids and probiotics, allows improving production indicators such as poultry livability, live weight gain, feed conversion and increasing the economic efficiency of meat production. The purpose of the research was to evaluate the eff ect of diff erent levels of arginine in poultry diets on average daily live weight gain and determine its effect on the livability of broiler chickens, optimize the amino acid composition of broiler chicken diets in accordance with the recommended standards of essential amino acids. The experiment was conducted at Kuratye LLC in Verkhniy Tagil in the Sverdlovsk region on the cross Arbor Acres of broiler chickens. The diet of the experimental group of broilers diff ered from the control diet in that the content of the amino acid arginine was brought to the standards recommended by German poultry farmers. It was found that the average daily live weight gain of broilers in the experimental group for the entire growing period was 47.7 g in the control group, in the experimental group it was 9.0 % higher than in the control and was 52.0 g. The European productivity index, calculated taking into account live weight, livability, fattening period of poultry and costs per unit of production in the control group was 274.0, and in the experimental group it was 309.2 units. From the conducted studies it follows that it is necessary to take into account the ratio of arginine to lysine in the diets of broiler chickens at the level of 1.04, 1.06 and 1.07, respectively, for the rearing phases such as starting, growth and finishing periods.

Meat productivity and meat quality of broiler chickens when input plant-based additive in compound feed

At present, the increase in poultry meat production is associated with the development of resource- saving technologies that ensure maximum productivity. However, the search for new methods to reduce the rearing period, increase live weight and reduce feed costs per 1 kg of gain continues. It should be noted that at the current stage of development, poultry farming has a high productivity potential, which is currently not fully utilized in some cases. The main reason is the inclusion of ingredients containing anti-nutritional substances in the compound feed. In compound feed for broiler chickens, the need to use biologically active additives has been proven. The inclusion of such additives in the composition of compound feed helps to increase the productive action, improve digestibility and effi ciency of using nutrients in diets. The purpose of the research was to study the meat productivity and meat quality of broiler chickens when input additive of plant-based additive in compound feed. On the base of research data, the eff ectiveness of including the plant-based feed additive Biostrong 510 in the compound feed for rearing of broiler chickens of the cross Cobb 500 in the amount of 0.010 %, 0.015 and 0.020 % of the compound feed weight was established. It made possible to increase the average daily live weight gain by 4.3–8.5 %, increase the gutted carcass weight by 4.3–4.6 %, reduce feed costs per 1 kg of broiler chicken gain by 0.07–0.13 kg, and increase the profi tability level by 0.78–1.44 abs.%. The safety of the tested additive was proven by a number of indicators. The obtained results of animal science, physiological and biochemical studies made it possible to identify the optimal dose of the plant feed additive Biostrong 510 for broiler chickens of the cross Cobb 500 in feeding technology 0.015 % of the mass of the compound feed.

Zhi-Kang-Yin formula attenuates high-fat diet-induced metabolic disorders through modulating gut microbiota-bile acids axis in mice

BackgroundMetabolic disorders have become one of the global medical problems. Due to the complexity of its pathogenesis, there is still no effective treatment. Bile acids (BAs) and gut microbiota (GM) have been proved to be closely related to host metabolism, which could be important targets for metabolic disorders. Zhi-Kang-Yin (ZKY) is a traditional Chinese medicine (TCM) formula developed by the research team according to theory of TCM and has been shown to improve metabolism in clinic. However, the underlying mechanisms are unclear.Aim of the studyThis study aimed to investigate the potential mechanisms of the beneficial effect of ZKY on metabolism.MethodsHigh-fat diet (HFD)-fed mice were treated with and without ZKY. The glucose and lipid metabolism-related indexes were measured. BA profile, GM composition and hepatic transcriptome were then investigated to analyze the changes of BAs, GM, and hepatic gene expression. Moreover, the relationship between GM and BAs was identified with functional gene quantification and ex vivo fermentation experiment.ResultsZKY reduced weight gain and lipid levels in both liver and serum, attenuated hepatic steatosis and improved glucose tolerance in HFD-fed mice. BA profile detection showed that ZKY changed the composition of BAs and increased the proportion of unconjugated BAs and non-12-OH BAs. Hepatic transcriptomic analysis revealed fatty acid metabolism and BA biosynthesis related pathways were regulated. In addition, ZKY significantly changed the structure of GM and upregulated the gene copy number of bacterial bile salt hydrolase. Meanwhile, ZKY directly promoted the growth of Bifidobacterium, which is a well-known bile salt hydrolase-producing genus. The ex vivo co-culture experiment with gut microbiota and BAs demonstrated that the changes of BAs profile in ZKY group were mediated by ZKY-shifted GM, which led to increased expression of genes associated with fatty acid degradation in the liver.ConclusionOur study indicated that the effect of ZKY on improving metabolism is associated with the modulation of GM-BAs axis, especially, by upregulating the abundance of bile salt hydrolase-expression bacteria and increasing the levels of unconjugated BAs. This study indicates that GM-BAs axis might be an important pathway for improving metabolic disorders by ZKY.

The essential oil blend Agolin Ruminant L reduces methane production in vitro and in vivo when included in the drinking water of cattle.

Two experiments were conducted to determine the potential for the essential oil blend Agolin Ruminant L (Agolin) to reduce enteric methane (CH4) emissions from beef cattle when delivered via the drinking water. Experiment 1 evaluated aqueous solutions of Agolin (50mg/L) and a non-protein nitrogen and mineral solution (uPRO ORANGE [uPRO]; 1.7mL/L) individually and in combination, where Agolin was added to concentrated uPRO at 3, 4.5, 6% Agolin (w/w) prior to dilution with water at 1.7mL/L, for a total of five treatments. These were incubated for 48h with a medium-quality Rhodes grass (Chloris gayana) hay substrate, with gas production, CH4 concentration in gas, and digestibility measured in vitro. In Exp. 2, Droughtmaster steers (n = 24) were fed a basal diet of Rhodes grass hay and were allocated to one of three water treatments (n = 8/treatment) supplemented with either uPRO (2.27mL uPRO/L water), or one of two inclusion rates of Agolin in combination with uPRO (2.27mL uPRO and 6 µL Agolin/L water or 2.27mL uPRO and 24 µL Agolin/L water) with enteric CH4 emissions, feed and water intake, and live weight gain (LWG) measured over 56 d. In Exp. 1, the inclusion of Agolin in uPRO at 6% w/w resulted in a reduction in CH4 production (15.8%; P = 0.003) and the proportion of CH4 in the gas produced (24.5%; P < 0.001). In Exp. 2, steers consuming the lower quantity of Agolin via drinking water had a 16.4% (P = 0.0027) reduction in CH4 production over the experiment, declining from 140g/d during week 1 to 117g/d in week 8. This inclusion rate of Agolin in the drinking water also resulted in a 25g (17.6%) CH4/d decrease in emissions by steers compared to control steers (P = 0.0205). However, no significant differences in CH4 yield (g CH4/kg dry matter intake), or CH4 intensity (g CH4/kg LWG) by steers was observed between treatments. These results demonstrate that Agolin reduces CH4 emissions when mixed in aqueous solution under in vitro and in vivo conditions, providing a potential method to reduce enteric CH4 emissions from cattle in extensive production systems.

Lactic acid bacteria supplementation: A bioprotective approach to mitigating cadmium-induced toxicity and modulating gene expression in murine models

This study aimed to assess the effects of different strains of lactic acid bacteria, namely LeviLactobacillus brevis (AC10), Lacticaseibacillus rhamnosus (AC11), and Pediococcus acidilactici (AC15), on mice exposed to cadmium-induced oxidative stress. The study assessed weight gain, liver enzymes, antioxidant enzymes, immunoglobulin factors, lipid peroxidation, and gene expression in liver and brain of mice. The findings revealed that the AC10 and AC11 strains had a higher ability to absorb Cd as compared to AC15. The in vivo analysis demonstrated that the dietary dual supplementation of AC10 and AC11 resulted in significant (p < 0.05) improvements, including increased body weight and food intake, reduced cadmium tissue deposition, decreased lipid peroxidation, enhanced cellular antioxidant redox potential, suppressed inflammation genes in the liver and brain tissues, and improved morpho-characteristics of the jejunum in mice challenged by cadmium-induced toxicity. The multiple mechanisms of action, including heavy metal sequestration, antioxidant enhancement, and maintenance of intestinal integrity, highlight the potential of these probiotics’ intervention as a viable approach to counteract the deleterious effects of cadmium exposure.

The Effects of Corn Silage and Sunflower Silage and Their Mixtures at Different Levels on the Fattening Performance of Kıvırcık Lambs

Maize silage is widely used in the nutrition of ruminants worldwide. Nevertheless, maize is expensive and difficult to grow in cold and arid climates. To investigate the effects of feeding pure maize silage and different mixtures of maize and sunflower silages on the fattening performance of lambs. In the study, 40 Kıvırcık male lambs aged 2.5-3 months and with an average live weight of 23-25 kg were used as the animal material. The experiment was carried out for 56 days in a semi-open barn. In accordance with the experimental design, animals were divided into 5 different silage groups (100 maize silage, 75 maize silage+25 sunflower silage, 50 maize silage+50 sunflower silage, 25 maize silage+75 sunflower silage, 100% sunflower silage), so that there would be 8 lambs in each group. The lambs were housed in individual compartments during the experiment and fed individually. Silages were given ad libitum, and additional 700, 900, and 1,400 g concentrated feed was given daily between the 0th and 21st, 21st and 42nd, and 42nd and 56th days, respectively. During the fattening period, the values of live weight, daily live weight gain, feed consumption, and feed conversion ratio were determined. The differences among the live weight values of different silage groups during the trial period were found to be not significant (p&gt;0.05), and the values obtained at the end of 8 weeks varied between 35.94 and 37.44 kg. The silage consumption of lambs fed different silage types showed significant differences (p