Liquid Laser Research Articles

Detection of Antimicrobial Proteins/Peptides and Bacterial Proteins Involved in Antimicrobial Resistance in Raw Cow's Milk from Different Breeds.

Proteins involved in antibiotic resistance (resistome) and with antimicrobial activity are present in biological specimens. This study aims to explore the presence and abundance of antimicrobial peptides (AMPs) and resistome proteins in bovine milk from diverse breeds and from intensive (Pezzata rossa, Bruna alpina, and Frisona) and non-intensive farming (Podolica breeds). Liquid atmospheric pressure matrix-assisted laser desorption/ionization (LAP-MALDI) mass spectrometry (MS) profiling, bottom-up proteomics, and metaproteomics were used to comprehensively analyze milk samples from various bovine breeds in order to identify and characterize AMPs and to investigate resistome proteins. LAP-MALDI MS coupled with linear discriminant analysis (LDA) machine learning was employed as a rapid classification method for Podolica milk recognition against the milk of other bovine species. The results of the LAP-MALDI MS analysis of milk coupled with the linear discriminant analysis (LDA) demonstrate the potential of distinguishing between Podolica and control milk samples based on MS profiles. The classification accuracy achieved in the training set is 86% while it reaches 98.4% in the test set. Bottom-up proteomics revealed approximately 220 quantified bovine proteins (identified using the Bos taurus database), with cathelicidins and annexins exhibiting higher abundance levels in control cows (intensive farming breeds). On the other hand, the metaproteomics analysis highlighted the diversity within the milk's microbial ecosystem with interesting results that may reflect the diverse environmental variables. The bottom-up proteomics data analysis using the Comprehensive Antibiotic Resistance Database (CARD) revealed beta-lactamases and tetracycline resistance proteins in both control and Podolica milk samples, with no relevant breed-specific differences observed.

Microstructures and mechanical properties of W-Co-Al tungsten heavy alloy prepared by different formation processing

High density, high strength and high susceptibility to adiabatic shear banding (ASB) have always been pursued by tungsten heavy alloys (WHAs) for kinetic energy armor-piercing projectiles. To meet the demand, this work reported a novel tungsten heavy alloy with high-density L12 precipitation-strengthened Co9Al9W binder (WCAW). WCAWs were prepared by liquid phase sintering (LPS) and laser ultrashort-time liquid phase sintering (LULPS) respectively. Experimental results show that LULPS technique with large molten pool convection and rapid solidification can produce WCAWs with uniform W distribution, low W-W contiguity and fine W particles. Conversely, W particles in LPS WCAWs were excessively connected and segregated. The uniform distribution, low W-W contiguity and fine W particles enable LULPS WCAW to exhibit better mechanical properties. The ultimate compressive strength of the LULPS WCAWs is ∼2442 MPa, 16.4 % higher than that of the LPS WCAWs. As well, the dynamic yield strength of LULPS WCAWs is high, which is ∼2281 MPa and 37.4 % higher than that of LPS WCAWs.

Comparison between Liquid Immersion, Laser Diffraction, PDPA, and Shadowgraphy in Assessing Droplet Size from Agricultural Nozzles

Spray droplet diameters play a key role in the field of liquid plant protection product (PPP) application technology. However, the availability of various measurement techniques, each with its unique operating principles for evaluating droplet size spectra, can lead to different interpretations of spray characteristics. Therefore, in this study, four measurement techniques—Liquid Immersion (LI), Laser Diffraction (LD), Phase Doppler Particle Analysis (PDPA), and Shadowgraphy (SG)—were utilized to evaluate the droplet size distribution of agricultural spray nozzles. Additionally, PDPA and SG were used to assess the average velocity of spray droplets. Experiments were conducted in three different laboratories with the main aim of comparing results obtained with various types of equipment utilized under ordinary practical conditions. Spraying tests were carried out using three flat fan nozzles and an air-induction flat fan nozzle. As a general trend, the lowest values for droplet diameters were measured using the Laser Diffraction technique, followed by Shadowgraphy. The PDPA technique provided the highest values for mean diameters (D10, D20, and D30) and the numeric median diameter (Dn0.5), whereas the Liquid Immersion method yielded the highest values for the Sauter mean diameter (D32) and volumetric diameters (Dv0.1, Dv0.5, and Dv0.9). Importantly, all measurement techniques were able to discriminate the four nozzles based on their Dv0.5 diameter. Average droplet velocities showed a similar pattern across the four nozzles with the PDPA and the SG measurement techniques. The differences in diameter values observed with the four measurement techniques underline the necessity of always including reference nozzles in spray quality assessments to base classifications on relative rather than absolute values.

Behaviors of laser-induced damage of dye cell windows at low fluences and their mechanism analysis

In most liquid dye lasers, dye cell windows are the optical components most susceptible to laser damage even at very low fluences. Although various mechanisms and mitigation techniques have been explored, damage still occurs at some point, and some behaviors of the process cannot be effectively explained. In this work, we investigated three special behaviors of dye cell window damage which cannot be explained by ordinary laser-induced damage mechanisms. We proposed that damage to dye cell windows can be caused by a contamination process similar to laser-induced contamination (LIC) on space optics, and used the LIC mechanism to analyze the cause of those damage behaviors. Additional experiments and calculations were conducted, providing more evidence in support of the analysis. In addition, it was found that dye molecules, which are necessary for dye lasers, are the primary contaminants in the LIC process. This explains the inevitable damage to dye cell windows.

A new longitudinal pumping scheme that uses elliptical laser beams to improve the compactness and efficiency of high-power dye lasers

Liquid dye lasers are intrinsically suitable for high-average-power operation because their flowing laser gain media can effectively remove the heat produced by laser amplification; thus, liquid dye lasers have a significant place in various laser applications. However, to sufficiently remove the dye solution heated by the laser, a very cumbersome dye flow system is required to achieve a high flow velocity in the dye cell of a high-power dye laser, which limits its applications. In this paper, we found that the pressure and power required to drive the high-speed dye flow are both proportional to the square of the laser beam size along the direction of the flow. Based on this result, we proposed a novel longitudinal pumping scheme that uses elliptical laser beams. This new scheme can significantly reduce the size and energy consumption of dye flow loops in high-power dye lasers. We also developed a dye laser system with a main amplifier using a 1 mm × 4 mm elliptical input laser beam. The amplifier produced an average power of 135 W with a repetition rate of 10 kHz and a conversion efficiency of 39.5%. The M2 factor of the output beam was better than 1.7. The flow parameters to drive the dye flow loops were 2.1×105 Pa, and 0.43 L/s, which gave a clearing ratio of 2 for the amplifier. This new pumping scheme enables high-power dye lasers to be more compact and efficient, making them more flexible for various applications.

Polarized emission of Cs3Cu2I5 nanowires embedded in nanopores of an anodic aluminum oxide template.

Due to the intrinsic polarized emission property, polarized emissive materials with anisotropic nanostructures are expected to be potential substitutes for polarizers. Herein, by the template-assisted strategy, well-aligned lead-free metal halide Cs3Cu2I5 nanowire (NW) arrays are fabricated by evaporating the precursor ink in the anodic aluminum oxide (AAO) for polarized emission. The Cs3Cu2I5/AAO composite film emits highly polarized light with a degree of polarization (DOP) of 0.50. Furthermore, by changing the molar ratio of CsI/CuI, the stability of Cs3Cu2I5 precursor inks is improved. Finally, an ultraviolet (UV) light-emitting diode (LED) is adopted to pump the composite film to achieve a blue LED device. The reported Cs3Cu2I5/AAO composite film with highly polarized light emissions will have great potential for polarized emission applications such as liquid crystal display backlights, waveguides, and lasers.

Photodynamic Therapy Combined with Liquid Nitrogen Cryotherapy and Curettage for the Treatment of Recalcitrant Periungual and Subungual Warts: Clinical Experience and Literature Review.

Warts are caused by human papillomavirus (HPV) infection and can involve multiple parts of skin and mucosa, of which periungual and subungual warts are the most difficult to treat. Periungual or subungual wart is verruca vulgaris growing around or under the fingernail, destroying and deforming the nail and nail bed. Currently, liquid nitrogen cryotherapy and CO2 laser are often used for the treatment. Clinically, few doctors routinely use photodynamic therapy (PDT) to treat viral warts. We used PDT combined with liquid nitrogen cryotherapy and curettage to successfully treat a case of intractable periungual and subungual warts.

(Invited) Electrodeposition of In and Ga from Non-Aqueous Electrolytes

Indium and gallium are metals whose applications have increased during the last decades. Both metals are primarily used in the form of binary, tertiary or quaternary (semi)conductor compounds in integrated circuits, photovoltaics, liquid crystal display devices, lasers and light-emitting diodes.In the past, the electrodeposition of indium and gallium has been investigated from both aqueous and non-aqueous electrolytes. Due to the negative standard reduction potentials of the two metals (E°In(III)/In(0) = −0.34 V and E°Ga(III)/Ga(0) = −0.53 V), their electrodeposition from aqueous electrolytes is hampered. This is especially true for gallium. In this talk, the electrodeposition of indium and gallium was investigated from several organic solvents. The investigated electrolytes are 1,2-dimethoxyethane (DME), polyethylene glycol (PEG), dimethyl sulfoxide (DMSO), and acetonitrile, containing chloride, bistriflimide, or methanesulfonate-based indium or gallium precursors.The electrochemistry and the electrodeposition of indium and gallium was studied using various electrochemical methods in combination with quartz crystal microbalance (QCM) and rotating (ring) disk electrodes. DMSO-based electrolytes enabled the electrodeposition of indium at room temperature and above its melting point (159.6 °C), while DME electrolytes gave rise to gallium electrodeposits composed of small liquid gallium spheres (Fig 1). In all the investigated electrolytes, the formation of intermediate monovalent indium(I) or gallium(I) species during reduction and oxidation was demonstrated. These species are unstable and react away via disproportionation reactions. Yet, it was found that the extent of their formation and their stability depends on the nature of the electrolyte. In addition, the electrodeposition of indium antimonide (InSb) films and eutectic gallium indium (EGaIn) liquid metal lines and patterns will be discussed, as well as the electrochemical synthesis of spherical indium nanoparticles.

The use of salts, buffers and surfactants in LAP-MALDI MS

Biological samples such as tissue extracts and enzymatic assays typically have a complex composition, which can interfere with analyte ionisation and detection in mass spectrometry (MS). Ionisation techniques such as electrospray ionisation (ESI) are often coupled online to an upfront chromatographic separation, whereas sample preparations for techniques such as conventional matrix-assisted laser desorption/ionisation (MALDI) are performed offline and, in the case of MALDI, rely on sample clean-up owing to different crystallisation behaviour. Liquid atmospheric pressure matrix-assisted laser desorption/ionisation (LAP-MALDI) MS is a hybrid ionisation technique that has been previously used to analyse a wide range of biological samples at fast acquisition rates. Here we report data from a systematic investigation of the influence of various buffer compounds, salts, surfactants, and other compounds necessary for biological sample preparation reflected in the signal intensity of a standard peptide mixture. Tricine showed the least signal reduction from the buffer compounds tested as did octyl-β-D-glucopyranoside for the surfactants. It can be concluded that LAP-MALDI MS can be used to analyse biological samples directly without major sample clean-up if their content of additives is not too high.

Comparison of mechanical dermabrasion and CO2 laser in cultured epithelial sheets transplantation in the treatment of refractory stable vitiligo: A multicenter, prospective and self‐controlled comparative study

AbstractBackgroundVitiligo is an acquired depigmented disorder that causes great damage to patient's appearance and mental health. Surgical treatment plays a vital role in patients with refractory vitiligo. Recipient‐site preparation (such as liquid nitrogen, mechanical dermabrasion, chemical peeling and laser) is a key step in surgical treatment. Among them, mechanical dermabrasion and CO2 laser can achieve accurate ablation in different parts and depths.ObjectivesTo compare the advantages and disadvantages of mechanical dermabrasion and CO2 laser and their preference for different parts in cultured epithelial sheets transplantation for the treatment of vitiligo.MethodsWe used a self‐controlled study to compare the efficacy of mechanical dermabrasion and CO2 laser in 120 stable vitiligo patients of different parts at different time points after cultured epithelial sheets transplantation.ResultsNo overall difference between the mechanical dermabrasion group and the CO2 laser group. While the recovery rate of different parts of the mechanical dermabrasion group was significantly different (p < 0.05). Two methods have distinct efficacy for different parts, and CO2 laser is better at treating uneven parts.ConclusionsBoth mechanical dermabrasion and CO2 laser have good results in cultured epithelial sheets transplantation for the treatment of vitiligo.

Acquisition and Control System for Flowing Liquid Laser

Acquisition and Control System for Flowing Liquid Laser

Improving hydroxyapatite coating ability on biodegradable metal through laser-induced hydrothermal coating in liquid precursor: Application in orthopedic implants.

During the past decade, there has been extensive research toward the possibility of exploring magnesium and its alloys as biocompatible and biodegradable materials for implantable applications. Its practical medical application, however, has been limited to specific areas owing to rapid corrosion in the initial stage and the consequent complications. Surface coatings can significantly reduce the initial corrosion of Mg alloys, and several studies have been carried out to improve the adhesion strength of the coating to the surfaces of the alloys. The composition of hydroxyapatite (HAp) is very similar to that of bone tissue; it is one of the most commonly used coating materials for bone-related implants owing to favorable osseointegration post-implantation. In this study, HAp was coated on Mg using nanosecond laser coating, combining the advantages of chemical and physical treatments. Photothermal heat generated in the liquid precursor by the laser improved the adhesion of the coating through the precipitation and growth of HAp at the localized nanosecond laser focal area and increased the corrosion resistance and cell adhesion of Mg. The physical, crystallographic, and chemical bondings were analyzed to explore the mechanism through which the surface adhesion between Mg and the HAp coating layer increased. The applicability of the coating to Mg screws used for clinical devices and improvement in its corrosion property were confirmed. The liquid environment-based laser surface coating technique offers a simple and quick process that does not require any chemical ligands, and therefore, overcomes a potential obstacle in its clinical use.

80-μs pulsewidth from a circulation-free diode-pumped liquid organic laser limited by thermal blooming.

Continuous-wave laser emission is challenging to obtain in organic lasers, whether in the solid or liquid form, a limitation caused by long-lived triplet states and by thermal effects. In liquid dye lasers, both issues can be fixed by rapidly flowing the dye, which is technically complex and prevents those lasers to be further miniaturized or easily integrated. Here we address the issue of the maximal pulsewidth that can be obtained in liquid dye lasers in the absence of any dye flow, in a compact and cost-effective diode-pumped laser system. Pulses as long as 80 μs have been obtained, thanks to the combination of a hemispherical resonator design, almost insensitive to thermal-lens effects, and an intentional mismatch between pump and cavity spatial modes. The limitation in pulse duration is shown to be entirely due to thermal blooming, and more specifically to diffraction losses brought by the spherical aberration of the thermal lens.

P60 Intralesional steroids vs. liquid nitrogen cryotherapy vs. 595 nm pulsed dye laser in the management of chest keloids: a comparative study

Abstract Chest keloids often result in cosmetic disfigurement, contractures, pruritus and pain. Inflammatory truncal acne often triggers chest keloid. Management is often challenging as they are relatively resistant to treatment and recurrence rates are high. The objective of the study was to compare the clinical efficacy and safety of intralesional triamcinolone acetonide (40 mg mL−1) vs. liquid nitrogen cryotherapy vs. 595 nm pulsed dye laser in the management of chest keloids. We conducted a comparative prospective study in a total of 42 patients with chest keloids from July 2021 to July 2022. These patients were randomly divided into three treatment groups via the envelope method. The Vancouver Scar Scale (VSS) was used to assess the clinical efficacy of the treatment modality. Patients were scored at the initiation of therapy and every 4 weeks until 16 weeks. Seven patients were lost to follow-up (response rate 83%). Treatment arms consisted of intralesional triamcinolone acetonide (n = 10), liquid nitrogen cryotherapy (n = 11) and pulsed dye laser (n = 14). There was a near-equal sex distribution of chest keloids (male-to-female ratio 1.21 : 1), with 15 patients (35.7%) having additional keloids in other body parts. The mean (SD) age of patients with chest keloids was 27.2 (13.6) years with a common incidence in those aged 20–30 years. Comparison of mean VSS as baseline and at 16 weeks revealed a statistically significant decrease in the score in all three treatment arms (P < 0.05). Comparison between the three different treatment arms revealed pulsed dye laser to be the most effective modality in decreasing VSS at the 16-week follow-up (Anova followed by post hoc analysis with Tukey’s test). With regard to the adverse clinical event profile, acneiform eruptions were reported following steroid injections, blistering and depigmentation were reported following liquid nitrogen cryotherapy, and blistering was reported following pulsed dye laser therapy. Thus, the pulsed dye laser (595 nm) appears to be a safe and efficacious treatment modality in the management of chest keloids.

Ex situ and in situ functionalized Yb/Fe nanoparticles obtained by scanning pulsed laser ablation in liquids: A route to obtain biofunctionalized multiplatform contrast agents for MRI and CT imaging

Two distinct strategies were used to improve the colloidal properties of hybrid Yb/Fe oxide NPs previously prepared by the pulsed liquid laser ablation process for use as a contrast agent in medical imaging.First, an exhaustive optimization process of the laser ablation synthesis parameters was carried out to reduce the hydrodynamic diameters of the Yb/Fe NPs. The hydrodynamic size was successfully reduced to <200 nm, thereby decreasing the polydispertivity index.Second, ex situ and in situ functionalization processes using glutathione, cysteamine, or polyethylenimine as capping agents have been developed to increase their colloidal stability at physiological pH values. Transmission electron microscopy, dynamic light scattering, Z-potential measurements, and Fourier Transform Infrared spectroscopy were used to examine the structure, morphology, colloidal and surface properties of Yb/Fe NPs.Colloidal stability of the Yb/Fe NPs as well as the linkage mechanism of functionalization have been studied extensively. This last parameter provides a critical information for subsequently bioconjugations in biomedical applications. Additionally, the biocompatibility of the Yb/Fe NPs was evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) experiments. These results indicate more appropriate colloidal characteristics and higher biocompatibility for ex situ-functionalized Yb/Fe NPs, especially when the capping agent is glutathione. Additionally, these Yb/Fe NPs show good magnetic resonance imaging and X-ray computerized tomography imaging abilities, thereby indicating promising potential as dual contrast agents.

Transmissive Mode Laser Micro-Ablation Performance of Ammonium Dinitramide-Based Liquid Propellant for Laser Micro-Thruster.

The transmissive mode laser micro-ablation performance of near-infrared (NIR) dye-optimized ammonium dinitramide (ADN)-based liquid propellant was investigated in laser plasma propulsion using a pulse YAG laser with 5 ns pulse width and 1064 nm wavelength. Miniature fiber optic near-infrared spectrometer, differential scanning calorimeter (DSC) and high-speed camera were used to study laser energy deposition, thermal analysis of ADN-based liquid propellants and the flow field evolution process, respectively. Experimental results indicate that two important factors, laser energy deposition efficiency and heat release from energetic liquid propellants, obviously affect the ablation performance. The results showed that the best ablation effect of 0.4 mL ADN solution dissolved in 0.6 mL dye solution (40%-AAD) liquid propellant was obtained with the ADN liquid propellant content increasing in the combustion chamber. Furthermore, adding 2% ammonium perchlorate (AP) solid powder gave rise to variations in the ablation volume and energetic properties of propellants, which enhanced the propellant enthalpy variable and burn rate. Based on the AP optimized laser ablation, the optimal single-pulse impulse (I)~9.8 μN·s, specific impulse (Isp)~234.9 s, impulse coupling coefficient (Cm)~62.43 dyne/W and energy factor (η)~71.2% were obtained in 200 µm scale combustion chamber. This work would enable further improvements in the small volume and high integration of liquid propellant laser micro-thruster.

Identification of Pseudomonas aeruginosa exopolysaccharide Psl in biofilms using 3D OrbiSIMS.

Secondary ion mass spectrometry (SIMS) offers advantages over both liquid extraction mass spectrometry and matrix assisted laser desorption mass spectrometry in that it provides the direct in situ analysis of molecules and has the potential to preserve the 3D location of an analyte in a sample. Polysaccharides are recognized as challenging analytes in the mass spectrometry of liquids and are also difficult to identify and assign using SIMS. Psl is an exopolysaccharide produced by Pseudomonas aeruginosa, which plays a key role in biofilm formation and maturation. In this Letter, we describe the use of the OrbiTrap analyzer with SIMS (3D OrbiSIMS) for the label-free mass spectrometry of Psl, taking advantage of its high mass resolving power for accurate secondary ion assignment. We study a P. aeruginosa biofilm and compare it with purified Psl to enable the assignment of secondary ions specific to the Psl structure. This resulted in the identification of 17 peaks that could confidently be ascribed to Psl fragments within the biofilm matrix. The complementary approach of the following neutral loss sequences is also shown to identify multiple oligosaccharide fragments without the requirement of a biological reference sample.

Influence of the position relationship between gas–liquid interface and laser focus on plasma evolution characteristics in jet LIBS technology

In order to understand the characteristics of breakdown process, plasma evolution and spectral emission in liquid jets laser-induced breakdown spectroscopy methods under the influence of the position variation between laser focus and gas–liquid interface, this work takes the plasma generated by laser-induced liquid jets as the object of study and discusses the changes in the spatial and temporal evolution characteristics and spectral radiation of the plasma when the position parameters between the laser focal point and the gas–liquid interface are different. The initial breakdown position is always between the front interface and the focus when the laser focus moves along the vertical direction of the interface, forming a phenomenon like ‘interface effect’. The relationship between laser pulse energy and breakdown probability exhibits a law similar to a ‘hysteresis curve’ in the study of breakdown threshold. In this work, plasma with smaller size, rounder shape, stronger radiation, higher temperature, and higher density can be produced when the focus position is in the liquid column 0.2 mm away from the front interface. Simultaneously, the spectral signal intensity and signal-to-back ratio of the characteristic peaks of target elements in water reach maximum values, and the spectral signal becomes more stable (relative standard deviation value reaches 2%). The Ca element’s ion radiation at 393.366 nm and atomic radiation at 422.673 nm are studied using narrow-band filtering imaging and time-space resolution spectroscopy. The findings demonstrate that the laws of ion and atomic radiation are not perfectly consistent in space and time.

Comparison of 3 Different Techniques in the Treatment of Pilonidal Sinus

Aim: Pilonidal sinus (Ps) is an infectious disease characterized by pain, swelling, redness and discharge. Minimally invasive interventions that allow the patient to return to daily life more easily. In this study, it was aimed to compare the surgical excision and primary suturing, liquid phenol application and laser application in Ps treatment. Materials and methods: The files of 358 patients diagnosed with Ps and operated in the years 2019-2022 were retrospectively evaluated. The patients were divided into 3 groups. Group 1 consisted of 125 patients who underwent surgical excision and primary suturing in 2019, group 2 of 194 patients who underwent liquid phenol in 2020-2021, and group 3 of 39 patients who underwent laser in 2022. The patients were evaluated at the postoperative 7th day, 1st month and 3rd month outpatient policlinic control. Results: A significant correlation was found between the groups and the development of infection (p&amp;lt;0.05). Infection was detected in 30.4% of those in group 1, 15.5% of those in group 2 and 10.3% of those in group 3. Recurrence was detected in 25 (20%) of 125 patients in group 1, 24 (12.2%) of 194 patients in group 2, and 3 (7.7%) of 39 patients in group 3. There was no statistically significant relationship between the groups and recurrence (p&amp;gt;0.05). Recurrence was seen in 14 (36.8%) of 38 patients in group 1, 18 (60%) of 30 patients in group 2, and 2 (50%) of 4 patients in group 3. A significant correlation was found between infection and recurrence (p&amp;lt;0.05). Recurrence is positive in 6.3% of infection-negative patients and 47.2% of infection-positive patients. Conclusion: Rapid recovery, short operative time, low complication and recurrence rates have increased the tendency for minimally invasive methods. The presence of infection may cause recurrence regardless of the method. Postoperative infection follow-up of patients is important to reduce recurrence rates.

Preparation and Characterization of Silver Nanoparticles by Pulsed Laser Ablation and Investigation of their Effect on Bacteria

Synthesis of silver nanoparticles (AgNP) by pulsed liquid laser ablation (PLAL) produces AgNPs that are better suited for biological applications compared to those prepared by standard wet-chemical methods. These were mainly achieved by water ablation with pulse widths in the nanosecond range. In the case of surface NP activation, we previously detected a strong surface-enhanced Raman scattering (SERS) signal from such AgNPs. To do this, we dilute Colloid slightly with LiCl. These surface alterations may have an effect on the NPs' capability to combat bacteria. every time, AgNPs with a median diameter of under ten nm were created., that has been incontestible in alternative studies to be the optimum size for germicidal activity.. Furthermore, minimum restrictive concentration (MIC) values for LiCl-modified AgNPs fell inside a slender vary of 1.1-3.8 g/mL, creating them the foremost efficient. This result's believed to be explained by the metal surface's enhanced surface reactivity, which is brought on by the existence of charged active sites.