In this study, the energy minimisation principle is used to map the self-organisation pattern of biphasic liquid–liquid co-flow in the mesodomain. In particular, we focus on the specific role of gravity in tuning the patterns. The total energy of the system expressed in terms of flow and geometric parameters is minimised by a multivariate non-linear method and the flow morphology corresponding to the minimised total energy is inferred for up, down and horizontal flow of the two phases. The analysis predicts the formation of stable segmented flow by the mechanisms of dripping, squeezing and jetting and also the transition to parallel flow with change in operating conditions and flow orientation. The predicted morphology and flow parameters are validated against experimental observations of the present study and also against available data from literature for mesoscale flow.