Objective: Sugarcane juice (Saccharum officinarum l.) is a natural source of liquid carbohydrates traditionally used for health and help with diabetes. The content of flavonoids, octacosanol, and saccharic compounds in sugarcane juice is proven to have antihyperglycemic activity, so it is safe to use by diabetics. Unfortunately, sugarcane juice is only stable for 4 h at room temperature. The study aimed to improve sugarcane juice's stability and obtain data on its antihyperglycemic effect. Methods: This research was conducted by making sugarcane juice, which was then carried out sterilization by microfiltration. The sugarcane juice is then packaged in a special sterile plastic. The results of sugarcane juice storage were then tested for stability at a certain time and antihyperglycemic effectiveness in mice. Results: The results showed that the stability of sugarcane juice can be extended up to 168 h. Sugarcane juice (Saccharum officinarum l.) at a dose of 5.6 mg/20 g Bodyweight, a dose of 11.2 mg/20 g Bodyweight, and a dose of 16.8 mg/20 g Bodyweight has an antihyperglycemic effect. Conclusion: Microfiltration techniques can be used to increase the shelf life of sugarcane juice and maintain its qualities as an antihyperglycemic for quite a long time.