Lipstick Samples Research Articles

Fourier transform infrared spectroscopy coupled with multivariate calibration for determination of carmine and rhodamine B levels in lipstick

Carmine is a natural colorant allowed to be used in cosmetics, while rhodamine is nonallowable. For some reason, carmine may be substituted with rhodamine to get economic profits. Therefore, some analytical methods are proposed and developed. This study developed fourier transform infrared (FTIR) spectroscopy in conjunction with utilizing multivariate calibration to determine carmine (CAR) and rhodamine B (RHB) levels in lipstick samples. Lipstick samples were placed directly into an attenuated total reflectance (ATR) crystal and scanned in the 4,000-650 cm-1 wavenumber range. The CAR and RHB levels were also determined using HPLC to be used as actual values. Partial least squares regression (PLSR) was used to stimulate the correlation between actual CAR and RHB values and predicted FTIR values. Based on optimization in terms of the selection of wavenumbers region, for CAR and RHB prediction modeling, absorbance values at wavenumbers of 1,357-673 cm-1 were used. The developed methods, besides having high validity, are rapid and exclusive of extensive solvents with minimum sample preparation; therefore, this technique is environmentally friendly

Investigation of mercury contamination in lipstick sold in the Saudi market and the potential health risk

Background: Environmental contamination is a significant global health issue, with cosmetics and pharmaceuticals being major polluters. High concentrations of heavy metals, such as Hg, have been found to have toxic effects and may pose a threat to human health. This study aimed to determine the concentration of mercury (Hg) in lipsticks available in the Saudi Arabia market. Methods: In this study, 12 lipstick samples from three colors were analyzed using inductively coupled plasma optical emission spectrometry (ICP-OES) to measure the content of Hg. Results: The concentration range of Hg was 0.004–0.296 ppm. Moreover, the systemic exposure dosage of mercury in the lipstick samples examined in this study ranged from 5.01 × 10−8 to 1.43 × 10−6 μg/kg bw/day, while the range of the margin of safety was from 7.3 × 109 to 2.2 × 108. Discussion: The Hg concentration in all analyzed samples was less than 0.50 and 1 ppm, which indicated that the Hg level was within acceptable limits according to Saudi Standards, Metrology and Quality Organisation (SASO) and the United States Food and Drug Administration (US FDA), respectively. On the other hand, the calculated margin of safety values for mercury exceeded the safe standard established by the WHO. The results derived from using hazard quotient (HQ) indices depict the potential carcinogenic health risk posed to consumers who employ red-colored lipsticks.

Analysis of Rhodamin B in Lipstick Preparations in Padangsidimpuan City by Thin Layer Chromatography (TLC) Method

Rhodamin B is a synthetic dye in the form of a crystalline powder, green or reddish-purple, odorless, and in solution will be bright red with fluorescence or fluorescence. Rhodamin B is commonly used as a dye in the textile and papermaking industries. Rhodamin B can cause irritation in short-term exposure and has carcinogenic effects. This study aims to find out whether there is Rhodamin B in lipsticks circulating in the Padangsidimpuan City market. Samples were taken by purposive sampling and 5 red samples were obtained. To identify the presence of Rhodamin B, a thin-layer chromatography method was used using eluene as the phase of motion, namely methanol, ethyl acetate, ammonia (50:20:25) and a 254 nm UV lamp The results of the study showed that out of 5 lipstick samples identified, there was one lipstick sample that was identified as containing Rhodamin B.

Assessment of Microbial Contamination and Metabolite Exposure in Cosmetic Products Used in Women's Beauty Salons.

An important route of microbial transmission is the shared use of these products in beauty salons. We aimed to investigate level of contamination with microorganisms and their metabolites in shared-use cosmetics in several women's beauty salons. Bacterial and fungal strains from 320 opened/used cosmetic samples were identified according to the Iranian standards for microbial quality of cosmetic products in Golestan Province (North of Iran) during Jul-Sep 2021. In order to assess production of toxins and protease by the predominant bacterial and fungal isolates, multiplex-polymerase chain reaction and the Lowry protein assay were performed, respectively. Microbial contamination was detected in 180 samples (56.5%), and the highest and lowest rates of microbial contamination were related to skin products (63.88%) and eye beauty products (20%), respectively. The highest level of S. aureus contamination (> 4,000 colony-forming units/g) was found in toner and face wash samples, and the highest level of C. albicans contamination was seen in lipstick samples (>20,000 colony-forming units/g). Only one (2%) S. aureus isolate produced staphylococcal enterotoxin B, while 3 out of 12 (25%) C. albicans isolates were able to produce protease. The shared-used health and beauty products, face products, in the study area are heavily contaminated. Therefore, it is essential to store used cosmetics in dry and cool places, establish strict inspection regulations for cosmetic products before and after entering the market, and increase awareness of beauty salon workers regarding the appropriate use, sanitary control, and maintenance of health and beauty products.

Right prefrontal activation associated with deviations from expected lipstick texture assessed with functional near-infrared spectroscopy.

There is a continuous consumer demand for ever superior cosmetic products. In marketing, various forms of sensory evaluation are used to measure the consumer experience and provide data with which to improve cosmetics. Nonetheless, potential downsides of existing approaches have led to the exploration of the use of neuroimaging methods, such as functional near-infrared spectroscopy (fNIRS), to provide addition information about consumers' experiences with cosmetics. The aim of the present study was to investigate the feasibility of a real-time brain-based product evaluation method which detects the incongruency between a product, in this case lipstick, and a consumer's expectations. Thirty healthy, female, habitual lipstick users were asked to apply six different lipsticks varying in softness and to rate the softness of and their willingness to pay (WTP) for each lipstick. Cerebral hemodynamic responses in frontal areas were measured with fNIRS during lipstick application and analyzed using the general linear model (GLM). Incongruency scores between softness and expectation were calculated in order to understand how far removed each lipstick was from a participant's optimal softness preference. The correlation between brain activation (beta scores) during the application of each lipstick and the respective incongruency scores from each participant were acquired using semi-partial correlation analysis, controlling for the effects of WTP. We revealed a significant intra-subject correlation between incongruency scores and activation in the right inferior frontal gyrus (IFG). This confirms that as the texture incongruency scores increased for the lipstick samples, activation in each individual's right IFG also increased. The correlation observed between incongruency perceived by participants and activation of the right IFG not only suggests that the right IFG may play an important role in detecting incongruity when there is a discrepancy between the perceived texture and the consumer's expectations but also that measuring activity in the IFG may provide a new objective measurement of the consumer experience, thus contributing to the development of superior cosmetics.

IDENTIFIKASI RHODAMIN B PADA LIPSTIK YANG BEREDAR DI KOTA BENGKULU DENGAN METODE SPEKTROFOTOMETRI UV-VISIBEL

The use of the dye rhodamine B as a color additive in cosmetics has been prohibited by the Regulation of the Head of the Food and Drug Monitoring Agency (BPOM) Number: HK.00.05.42.1018 concerning Additional Ingredients in Cosmetics, because rhodamine B has carcinogenic properties if used long term in the body. In this research, 10 lipstick samples were used purposively obtained from the market in Bengkulu City. Lipstick samples were selected based on the criteria that they did not meet BPOM distribution requirements. Determination of rhodamine B levels was carried out using the UV-Visible Spectrophotometry method. The maximum wavelength used in the identification of Rhodamine B is 554 nm. The test results of ten lipsticks showed that nine lipsticks contained rhodamine B, in lipsticks with codes L2, L3, L4, L5, L6, L7, L8, L9 and L10 with a percentage of 0.0058%-0.0242%, and only one sample that was identified negative, L1 with an absorbance value of 0.364 with a percentage of 0%. In this study it can be concluded that there were 9 samples of 10 lipstick samples that were identified as containing Rhodamine B quantitatively.

Solid phase microextraction of rhodamine B in cosmetic samples using ZnS@GO@WMCNTs Nanocomposite with Spectrophotometric Detection

Rhodamine B dye (RhB) has carcinogenicity, genotoxicity, neurotoxicity and toxicity on living cells and therefore its use is prohibited. but it is still secretly used in cosmetics and foods. For this reason, it is important to develop analytical methods for accurate and sensitive analysis of trace levels of RhB dye in cosmetic and food samples. In this study, a new solid phase microextraction method (SPME) was developed for quick and cheap analysis of RhB in cosmetic samples. A new nanohybrid material including graphene oxide (GO), multi-walled carbon nanotubes (MWCNTs) and zinc sulfide nanopartices (ZnS) was synthesized by hydrothermal synthesis method and the new ZnS@GO@WMCNTs nanohybrid material was used as sorbent in simultaneous separation and preconcentration of RhB from cosmetic products by SPME. ZnS@GO@WMCNTs material was characterized by Raman spectroscopy, scanning electron microscopy (SEM), SEM-Mapping and energy dispersive X-Ray fluorescence (EDX) techniques. The effects of experimental parameters such as amount of sorbent, pH of sample solution, sample volume, effects of matrix, eluent solutions on the recoveries of RhB were investigated. RhB dye adsorbed on ZnS@GO@WMCNTs was eluted with acetic acid–ethanol mixture (2:1) and determined by using UV–Visible spectrophotometer at 550 nm. The fact that RhB adsorbed quantitatively on the adsorbent at all pH values shows that strong Van der Waals interactions between the ZnS@GO@WMCNTs and RhB molecules are effective in adsorption. In optimal experimental conditions, the limit of detection (LOD) and preconcentration factor (PF) of the proposed MSPE-UV/Vis spectroscopy procedure were 4.74 and 44, respectively. The MSPE-UV/Vis procedure was successfully applied to the microextraction and determination of RhB in nail polishs ve lipstick samples with recoveries in the range 95–101 %.

Risk assessment of heavy metals in brands of lipsticks commonly used in Shahrekord, Iran

The desire for beauty and frequent use of cosmetics can expose humans to heavy metals, which can cause immune system disorders over time. We selected 5 common lipstick brands to determine the concentrations of cadmium (Cd), lead (Pb), and chromium (Cr). We prepared each sample by acid digestion, and then the concentration of the metals was measured using an atomic absorption spectrometer. The results showed that Pb, Cd, and Cr were present in all lipstick samples. However, their concentrations were lower than the Food and Drug Administration (FDA) standards. The maximum concentrations of Pb and Cd were 2.31 mg/kg (brand A) and 0.037 mg/kg (brand D), respectively. Health risk assessment of the examined metals showed that only Cr can pose non-carcinogenic (14.98) and carcinogenic (44.96E-04) risks to consumers. Despite the low concentration of heavy metals in our study, chronic use of lipstick can pose carcinogenic and non-carcinogenic risks due to the presence of Cr.

Inductively coupled plasma-mass spectrometry (ICP-MS) detection of trace metal contents of children cosmetics

Consumption of cosmetic products in daily life in order to improve skin quality or appearance is becoming quite common. Over time, it may expose consumers to skin problems and effects caused by the absorption of chemical elements. Clean cosmetic production is important for our health. However, heavy metals can be found as impurities in raw materials or as by-products of the cosmetic production process. Women's ingestion and exposure to organic and inorganic contaminants in cosmetics through dermal absorption, due to reasons such as personal habits and characteristics of the living environment, may contribute to carcinogenic risks following daily dietary exposure. NCS ZC 81002b (Human Hair) was performed as a certified reference material in method validation. This work was conducted to evaluate element concentrations of selected elements (Al, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Se, Hg, Cd and Pb) in 3 different brands of children's cosmetic products such as bright and colorful 22 eye shadows (7 different colour) 4 lipsticks (3 different colour) and 4 nail polishes (3 different colour) used by children in Turkey. The sample solutions were analyzed with the inductively coupled plasma mass spectrometry (ICP-MS) technique after the microwave digestion system. Consequently, the highest concentration of Al used as pigment was found in the eye shadow, lipstick and nail polish samples for the 3 brands, while the Se concentrations were the lowest in lipstick samples. The results of As, Cd, Hg, Pb, which are restricted or banned for use in cosmetic products in most countries, are below the limits set by the Food and Drug Administration (FDA) and Health Canada Product Safety Laboratory (PSL).

Comparative forensic discrimination of pink lipsticks using fourier transform infra-red and Raman spectroscopy

As a routine, lipstick is daily used by females and can easily be transferred to clothes, cups, tissue papers, and other objects. The analysis of lipsticks is a relatively new and exciting field in forensics, helping to identify suspects in criminal cases where lipstick evidence has been left at crime scenes. By matching a specific brand of lipstick to a sample, investigators can positively connect certain individuals to locations or people, helping to aid in their investigation and subsequent proceedings. In this present study, 20 different pink shade lipsticks of the same manufacturer were analyzed using Vacuum FT-IR, and Raman spectroscopy to show a differentiation percentage of 95.8% between the samples. Data analysis using data mining techniques was performed on FT-IR spectra. Principle Component Analysis (PCA) was used as a data mining model for classification purposes, and it was able to distinguish between lipsticks samples based on their FT-IR spectra.

Determination of Cd, Co, Cr, Ni and Pb metals in lipsticks samples by Flame atomic absorption spectrophotometry (FAAS)

The study was conducted this work to analyze levels of toxic metals in lipsticks specifically sold in the Bhopal area. Acid digestion process was used to prepare the samples and to quantify the levels of heavy metals, including Cd, Co, Cr, Ni, and Pb Flame Atomic Absorption Spectrophotometry was used. Calibration curves were prepared with good linearity for accurate measurement of concentrations of these metals. All heavy metal observed were within the allowable limit. To assess the health risks, Average daily dose (ADD) and Hazard quotient (HQ) (Ingestion) for all the lipstick samples were calculated. Among these samples, three samples shows higher values among them one belonged to a branded category, while the remaining two were from the non-branded category. These particular Lipsticks samples exhibited higher HQ values (higher than 1), indicating a probable hazard linked of toxic-metals.

Determination Of Some Trace Elements In Some Lipstick Products By Inductive Coupled Plasma-Mass Spectrometry

Cosmetics have been attracting the attention of humanity since it existed. Over the years, the reach of the cosmetics industry has increased exponentially. In recent years, cosmetic manufacturers have developed many formulations on cosmetic products that are brighter in color, more permanent and not easily affected by external factors. However, heavy metal powders are used while producing these formulations especially inside in dyestuffs and stabilizers. Cosmetic products can be absorbed through the skin and cause systemic toxicity. Hundreds of chemicals are used in many cosmetic products such as make-up products, shampoos, creams, aftershaves, and these chemicals may cause significant health problems. Thus, this study is designed to investigate the heavy metal content of lipstick samples which are used extensively by women. Determination of aluminum (Al), chromium (Cr), iron (Fe), nickel (Ni), copper (Cu), zinc (Zn), elements in 35 different lipstick samples (including 15 brands) were performed by Inductively Coupled Plasma-Mass Spectrophotometer (ICP-MS) device. Before the analysis, two different solubilization methods, wet solubilization and dry ashing, were applied to the samples in order to take the metals in the lipstick samples into inorganic environment

Toxic element determination in selected cosmetic products: health risk assessment.

The aim of this research was to determine the content of arsenic (As), cadmium (Cd), lead (Pb), and nickel (Ni) in selected lipsticks and face foundations of different price categories and the health risk assessment associated with these toxic elements via dermal and oral exposure. The samples (10 lipstick and 10 face foundations) were purchased from local markets in Belgrade, Serbia. The samples were prepared by acid digestion and analyzed by atomic absorption spectrometry (flame atomization for Cd, Pb, and Ni and electrothermal atomization for As). Arsenic was found in 50% of lipstick samples and in only two face foundation samples with the highest concentration of 0.28 mg/kg in the lipstick sample. Lead concentration was in the range of 1.15 to 5.12 mg/kg in ten samples. Nickel was found in five samples out of 20, with the highest concentration of 4.20 mg/kg. Cadmium was found in only one face foundation sample in the cheaper price range (1.11 mg/kg). The obtained results indicate that concentrations of As, Pb, Cd, and Ni were within the permissible limits according to Serbian legislation. Health risk associated with these elements was assessed as non-carcinogenic and carcinogenic risk. Obtained hazard quotients (HQs) and hazard indexes (HIs) were below 1, indicating the absence of significant non-carcinogenic health risk due to exposure to these toxic elements (TEs). The carcinogenic risk results show that investigated cosmetic products can be considered low-risky due to the possibility of causing malignant disease.

Development of A Liquid-Phase Microextraction Method for Simultaneous Determination of Parabens in Lipstick Samples at Trace Levels by High-Performance Liquid Chromatography.

The endocrine-disrupting potential of parabens, as well as their relation to cancer, has sparked significant discussions over their impact. Consequently, analyses of cosmetic products are an essential necessity, particularly in terms of human health and safety. In this study, a highly accurate and sensitive liquid-phase-based microextraction method was developed to determine the five parabens at trace levels by high-performance liquid chromatography. All prominent parameters of the method such as extraction solvent type and amount (1,2-dichloroethane/250μL), and dispersive solvent type and amount (isopropyl alcohol/2.0mL) were optimized to enhance the extraction efficiency of the analytes. The mobile phase consisting of 50mM ammonium formate aqueous solution (pH4.0) and acetonitrile (60:40, v/v) was used to elute the analytes at a flow rate of 1.2mLmin-1 in the isocratic mode. Analytical performance of the optimum method for methyl, ethyl, propyl, butyl and benzyl parabens were determined and the analytes recorded detection limit values of 0.78, 0.75, 0.34, 0.33 and 0.75μgkg-1, respectively. Four different lipstick samples were analyzed under optimum conditions of the developed method, and the amount of parabens quantified in the samples using matrix matched calibration standards was in the range of 0.11-1.03%.

Simultaneous electrochemical determination of trace zinc(II), cadmium(II) and lead (II) in lipsticks using a novel electrode covered with bismuth/over-oxidized poly(xylenol blue) film

The presence of zinc, cadmium and lead, which affects human health even at low concentrations, in different sample matrices is of great concern. This study proposes a dependable approach for the selective and sensitive identification of these cations with an over-oxidized poly(xylenol blue) film glassy carbon electrode coated with bismuth (Bi/PXBov.ox/GCE). Several techniques were used to chemically and physically evaluate the produced electrode surface. The following were chosen as the best conditions for determining these elements: 0.1 mol L-1 acetate buffer solution as supporting electrolyte (pH 4.5), −1.5 V accumulation potential, and 180 s accumulation duration. Under these conditions, the detection limits of Zn(II), Cd(II) and Pb(II) were found as 3.20 μg L-1, 0.21 μg L-1 and 1.98 μg L-1, respectively. Furthermore, this novel, cheap and easy-to-prepare Bi/PXBov.ox/GCE was also successfully used for the analysis of Zn(II), Cd(II) and Pb(II) in lipstick samples. The accuracy of the voltammetric measurements was assessed by comparing them to the data acquired with inductively coupled plasma mass spectrometry, and the results were found to be consistent.

Rapid and nondestructive analysis of lipstick on different substrates using ATR-FTIR spectroscopy and chemometrics.

Lipstick can be an important piece of evidence in crimes like murders, rapes, and suicides. Due to its prevalence, it can be an important corroborative evidence in crime reconstruction. The analysis of such evidence can provide an evidentiary link between the suspect, the victim, object, or the crime scene. We report the use of nondestructive ATR-FTIR spectroscopy combined with chemometrics for the classification of 10 brands of lipsticks with nine samples each. Chemometric method of partial least square-discriminant analysis (PLS-DA) has been employed to interpret the data and classify the samples into their respective classes. The PLS-DA model provides an AUC figure above 0.99 in all brands except one; for which it is slightly less at 0.94. We have also tested the traces of these lipstick samples on different substrates treating them as unknowns in the already trained PLS-DA model. 100% of the samples on nine substrates viz. a cotton, nylon, plastic, dry tissue, denim (blue jeans), wet tissue, nitrile gloves, white paper, and polyester were correctly attributed to their source brand. In conclusion, the results suggest that ATR-FTIR combined with the chemometrics is a rapid, nondestructive, and accurate method for the discrimination and source attribution of lipstick. This study has potential for use in actual forensic casework conditions.

Exposure and health risks of metals in imported and local brands' lipsticks and eye pencils from Bangladesh.

Elemental contamination in cosmetics is a serious health concern as it can pose a cumulative effect on the user's body over a long period. The prime motive of the study was to assess the concentration of 10 concerning chemical elements (Pb, Cd, Cr, As, Co, Ni, Cu, Zn, Fe, and Mn) in imported and local lipsticks and eye pencil samples collected from retail outlets in central Bangladesh (Dhaka city) and to assess their dynamic health risks for users. A total of 18 lipsticks and 24 eye pencils were studied and concentrations of chemical elements were examined with atomic absorption spectrophotometer. The health risk assessment was performed for dermal and ingestion routes of the contaminants. The results reveal that the concentrations of the examined elements vary with colors, brands, and origins. Pb and As concentrations were found below the permissible limit but Cr concentration in some samples exceeded the allowable limit in cosmetics. Cd was not detected in any samples; however, other examined elements such as Co, Ni, Cu, Mn, Zn, and Fe were detected in considerable concentrations. Elements like Mn, Zn, and Fe were found at high levels. In the case of lipstick samples, elemental concentrations followed the order of Fe > Zn > Mn > Ni > Cr > Cu > Pb > Co > As, while the order was Fe > Cu > Mn > Zn > Ni > Cr > Co > Pb > As for eye pencil samples. Results of the hazards quotient (HQ) indicate that there were no non-carcinogenic or carcinogenic risks of elements in samples for dermal exposure. But the cancer risk values of Cr (HQ > 1 for ingestion) in brown color lipsticks and Ni indicate that lipsticks have some carcinogenic effects if they enter the user's body. Dermal cancer risk for eye pencils has also been calculated and for Pb, Cr, Ni, and As; the values were found within the acceptable ranges of 10-6-10-4. It is suggested that the allowable limit of all toxic elements in cosmetics must be established. Furthermore, continuous monitoring is urgently needed for personal care products like lipsticks and eye pencils commonly available in the local markets in the country like Bangladesh.

ANALISIS KANDUNGAN LOGAM PADA LIPSTIK YANG BEREDAR DI KOTA BATUSANGKAR

Research has been conducted to analyze the content of heavy metals (Pb, Cd, Mn) in lipstick circulating in Batusangkar Market. Samples are lipsticks that are registered and not registered with BPOM with various brands, colors and prices sold in the batusangkar market and totaling 10 samples. The sample was destroyed using a mixture of nitric acid and hydrogen peroxide. Furthermore, the content of Pb, Cd and Mn was measured using Atomic Absorption Spectrophotometry (AAS). Based on the results of the analysis, the Pb content in the sample ranged from -6.875 mg/kg to 2.0305 mg/kg, Cd content 0.18 mg/kg to 0.52 mg/kmg/kg, Mn content -4.60 mg/kg up to 149.59 mg/kg. Lead was found in 4 out of 10 lipstick samples, heavy metal Cd was found in all lipstick samples and manganese was found in 8 out of 10 lipstick samples. Based on these results, it can be concluded that the heavy metal content in the electrical sample is below the specified limit so that it meets the requirements of the Regulation of the Head of the POM No. 17 of 2014 concerning requirements for microbial and heavy metal contamination in cosmetics.

Elemental analysis and characterization of vermilion and lipstick sample using SEM-EDS

Though many offenders are aware of the trail evidence typically used in forensic settings, there is a low possibility of translocation developing during a crime. Conventional evidence is rarely left at a crime scene, thus unconventional potential evidence must be taken into consideration. Lipstick and vermilion stains from cosmetics could be transferred from a criminal, crime scene, or victim. Perpetrators frequently aren't aware that such evidence could be utilized to trace a suspect to a crime scene because it's common for these cosmetic particles to be conveyed during a near-range individual assault and left out undetected. In this study, ten lipstick and ten vermilion color samples which are prominently found in crime scenes were analyzed by (SEM-EDS) scanning electron microscopy-energy dispersive X-ray spectroscopy to identify particles that might have been transferred during the close-quarters fight. EDS data shows constituents of the samples taken which can be further used as a data set to match such colored cosmetics samples in crime scenes. Mass percentage of C, O, Hg, As, Pb, Cr, Fe, Mg, Si, and Co shows variation in all the samples which provide uniqueness and served as a key factor in identification.

ANALISIS KEBERADAAN PEWARNA SINTETIS (RHODAMIN B) PADA LIPSTIK YANG DIJUAL DI PASAR DESA TANJUNG BATU KABUPATEN OGAN ILIR

Rhodamine B is a coloring agent whose use is prohibited in cosmetic products because it is used in coloring paper and textiles. The purpose of this study was to find out the description of the existence of the synthetic dye Rhodamine B in lipsticks sold at the Tanjung Batu village market, Ogan ilir district, in 2022 based on color, price and brand. The design used in this research is descriptive and the method used is purposive sampling. The inspection method used is the chromatographic method. The results of the research that was conducted on 10 samples found 1 sample (10%) positive and 9 samples (90%) negative. Based on the color of the lipstick, there were 2 samples (100%) which were bright red negative for Rhodamin B, 6 samples (100%) who were pink negative for Rhodamin B, and 4 samples of lipstick with brick red color, 1 sample of lipstick (25%) was positive for Rhodamin B and 3 samples (75%) were negative for Rhodamine B. Based on price ? Rp. 20,000, 5 samples (100%) were negative (-) containing Rhodamin B, while < Rp. 20,000, 1 sample (20%) was positive (+) containing Rhodamin B. Based on the brands of 10 lipstick samples sold in Tanjung Batu Village, District Ogan Ilir 2022 was studied and got 1 sample (20%) positive results with a brand that has a BPOM permit.