Lipid-soluble Vitamins Research Articles

Effect of Acrylamide on Ovaries of Female Albino Rats and Possible Protective Role of Vitamin E

Abstract Background: Acrylamide (ACR) is a chemical substance with a very wide range of uses and it accounts for one of the major health concern and one of the most important contam-inants occurring in foods heated at high temperatures so human exposure to ACR is high. Vitamin E is the most important lipid-soluble vitamin presents in the nature and one of the basic vitamins in biological systems and plays a vital role in many physiological processes. Aim of Study: To investigate the Potential protective effects of Vitamin E on Acrylamide induced ovarian damage in female albino rats through histological examination by light microscope. Material and Methods: This study was conducted in Human Anatomy and Embryology Department in Sohag University during 2019 forty-five female virgin albino rats weighing 180-300g were used and randomly divided into three equal groups: Control group; ACR group was given 10 mg/kg body weight of acrylamide for by oral gavage for 3 weeks; the third group was given ACR at a dosage of 10 mg/kg/d, and vitamin E at a dose of 100mg/kg body weight for 3 weeks orally then sacrificed by cervical dislocation Ovaries were excised for light microscopic study and mor-phometric study. Results: Oral administration of ACR induced marked cystic ovarian changes, degenerative changes in zona pellucida, Granulosa cells and oocytes detected by light microscope this study reported that supplementation of a diet with Vit E provided antioxidant defense against the toxicity of ACR in ovary. Conclusions: These results concluded that acrylamide combined with vitamin E in comparison with acrylamide induced less ovarian damage and this protective effect might be due to the antioxidant activity of Vit E.

Pharmacology and Pharmacokinetics of Vitamin E: Nanoformulations to Enhance Bioavailability.

Vitamin E belongs to the family of lipid-soluble vitamins and can be divided into two groups, tocopherols and tocotrienols, with four isomers (alpha, beta, gamma and delta). Although vitamin E is widely known as a potent antioxidant, studies have also revealed that vitamin E possesses anti-inflammatory properties. These crucial properties of vitamin E are beneficial in various aspects of health, especially in neuroprotection and cardiovascular, skin and bone health. However, the poor bioavailability of vitamin E, especially tocotrienols, remains a great limitation for clinical applications. Recently, nanoformulations that include nanovesicles, solid-lipid nanoparticles, nanostructured lipid carriers, nanoemulsions, and polymeric nanoparticles have shown promising outcomes in improving the efficacy and bioavailability of vitamin E. This review focuses on the pharmacological properties and pharmacokinetics of vitamin E and current advances in vitamin E nanoformulations for future clinical applications. The limitations and future recommendations are also discussed in this review.

Genetic engineering of potato (Solanum tuberosum L.) for enhanced α-tocopherols and abiotic stress tolerance.

Vitamin E (α-tocopherol) is a lipid-soluble essential vitamin recognized for improvement in degenerative health conditions, abating cancer risk, and coronary heart diseases in humans. While in plants, it acts as a free radical scavenger that protects cells against oxidative and photooxidative damages. The daily consumption of potato makes it a key target for biofortification with vitamins for eliminating vitamin deficiency in large populations. Vitamin E biosynthetic pathway genes have been overexpressed in plants via genetic engineering to enhance the α-tocopherol content. Major genes involved in the vitamin E biosynthesis in plants viz. the homogentisate-phytyltransferase (At-HPT) and γ-tocopherol-methyltransferase (At-γ-TMT), isolated from Arabidopsis were constitutively overexpressed in potato (Solanum tuberosum L.). The molecular analyses of independent transgenic lines revealed a stable integration of both the genes in the plant genome. The transgenic potato exhibited significantly improved vitamin E contents up to 173-258% in comparison to the untransformed control plants. Transgenic tissues also exhibited increased cellular antioxidant enzymes, proline, osmolyte, and glutathione content that are directly correlated with the ability of the plant to withstand abiotic stresses imposed by salt (NaCl) and heavy metal (CdCl2 ). Therefore, the current strategy of increasing the vitamin E content in potato with enhanced tolerance to abiotic stresses might greatly aid efforts to engineer crops for human health benefits and greater yield under adverse environmental conditions.

Effects of 2,4-thiazolidinedione (TZD) on milk fatty acid profile and serum vitamins in dairy goats challenged with intramammary infusion of Streptococcus uberis.

The study included two experiments. In the first, 24 lactating Saanen dairy goats received low-energy diet without vitamin supplements. Twelve goats received a daily IV injection of 2,4- thiazolidinedione (TZD), others received saline injection. A week later, 6 goats from each treatment were challenged with intramammary infusion (IMI) of saline (CTRL) or Streptococcus uberis. In the second experiment, 12 Saanen lactating dairy goats received supplemental vitamins to reach NRC recommendation level. Six goats in each group were injected with TZD or saline daily, and 14 d later received Streptococcus uberis IMI in the right half of the udder. The hypotheses were (1) TZD does not affect the level of retinol in blood, and (2) the fatty acid profile is affected by the interaction between mammary infection and TZD in dairy goats. In the first experiment blood samples were collected on d -7, -2, 1, 2, 12 and milk samples were collected on d -8, 1, 4, 7, and 12, both relative to IMI. In the second experiment, blood samples were collected on d -15, 0, 1, and 10 relative to IMI. Milk and serum samples were analyzed for retinol, α-tocopherol and fatty acid profile. Serum retinol and β-carotene concentrations were higher in the second experiment compared to the first. Serum β-carotene and α-tocopherol were greater in TZD than CTRL and there was a TZD × time interaction in the first experiment. In addition, the TZD × time interaction showed that the milk fatty acid were reduced in C16 : 0 while C18 : 3 n3 while total omega 3 fatty acids were increased, as well as with minor effect on preventing a transient increase in α-tocopherol in milk. Overall, the TZD may affect the lipid-soluble vitamins and fatty acid profile, potentially altering immune responses, during mastitis in dairy goats.

Pooling of monoclonal antibodies for the rapid detection of commercially important finfish

Fish are a valuable source of high-quality proteins, beneficial polyunsaturated fatty acids, and lipid-soluble vitamins in the human diet, but they also make up one of the “big eight” allergenic foods that are responsible for >90% of food allergy cases. A reliable and cost-effective analytical method is thus needed to detect fish protein in foods to protect fish-allergic individuals. Although immunoassays have been developed to detect many food allergens, there is no effective immunoassay capable of recognizing all common food fish species on the market. The selectivities of three monoclonal antibodies (mAbs), 8F5, 2G9 and 2F3, known to exhibit anti-finfish activity against cooked crude fish protein extract from red snapper, yellow fin tuna and swordfish, respectively, were each tested for 63 commercially important fish species using indirect enzyme-linked immunosorbent assays (iELISA). Based on the results, none of the mAbs showed cross-reactivity with all fish species tested; the cross reactivity patterns of these 3 mAbs complemented each other and none cross reacted with any non-fish samples. Pooling these mAbs (1:1:1) in an iELISA successfully detected a wide spectrum of commercially important fish species, including those subjected to various processing conditions. This pooled mAbs based iELISA exhibited high sensitivity, specificity and reproducibility, suggesting its potential utility as a qualitative assay for the regulatory enforcement of the food laws that protect fish sensitive consumers.

A Single Large Dose of Vitamin D Could be Used as a Means of Coronavirus Disease 2019 Prevention and Treatment.

There is no specific and effective medication for coronavirus disease 2019 (COVID-19), and avaccine is not available in recent months. Here, we hypothesize that a single large dose of vitamin D (Vit D) could be an option for trial in COVID-19. Vit D deficiency or insufficiency is very common in the general population as well as in patients with COVID-19. It has been shown that low Vit D level is associated with viral infection, and Vit D supplementation is beneficial for people infected with viruses, such as HIV and hepatitis C virus. Although COVID-19 is a respiratory disease, the morbidity and mortality of this disease are driven by coagulopathy. Clinical studies have shown that Vit D can exert anticoagulant effects. Vit D, a lipid-soluble vitamin, can be administered as a draught. Vit D supplementation is safe and has rare toxic events. In addition, the cost of Vit D is fairly low. Based on these observations, we speculate that a single dose of 300,000 IU Vit D may have a role in the prevention and treatment of COVID-19.

Effects of dietary vitamins supplementation level on the production performance and intestinal microbiota of aged laying hens

The objective of this study was to investigate the effects of higher vitamins supplementation level on the performance, immunity, and intestinal microbiota of old laying hens. Twelve birds were randomly chosen from 312 healthy, 65-wk-old Hy-Line Brown layers for sampling after a 7-wk acclimation period. The remaining 300 hens were randomly allocated to 1 of 4 dietary treatments for a 13-wk feeding trial: basal diet (CON), basal diet with 2-fold supplementation level of lipid-soluble vitamins (LV), 2-fold supplementation level of water-soluble vitamins (WV), or 2-fold supplementation level of both lipid-soluble and water-soluble vitamins (BV), respectively. Compared with 72-wk-old laying hens, the 85-wk-old laying hens showed declined egg quality, which implied by inferior eggshell strength and yolk color (P < 0.05). However, after 13 wks feeding trial, the birds in WV group had higher yellowness of yolk color, and LV group had increased laying rate (P < 0.05) compared with CON. Meanwhile, WV and/or BV groups showed improved GSH/GSSG levels in liver and increased secretory immunoglobulin A concentrations in jejunum compared with CON (P < 0.05). In addition, higher dietary vitamin supplementation levels significantly altered the composition of intestinal microbiota, as evidenced by increased abundance of ileal Lactobacillus, whereas reduced richness of ileal Romboutsia, Turicibacter, and cecal Faecalibacterium (P < 0.05) in WV group and increased cecal Megasphaera and Phascolarctobacterium (P < 0.05) in LV group compared with CON group. In conclusion, higher vitamin supplementation levels in the diet could improve laying performance and egg quality of aged hens, which was closely correlated with the increased abundance of beneficial microbiota in the intestine.

MTORC1 is required for the lipidation and ER‐to‐Golgi transport of prechylomicrons in Caco‐2 cells

Dietary fats are efficiently digested and absorbed by enterocytes, assembled into triacylglycerol‐rich chylomicrons and finally secreted in a multistep process. The role of mTORC1 (mechanistic target of rapamycin complex 1) in the fate of absorbed lipids, their intracellular trafficking, triacylglycerol packaging in chylomicrons by enterocytes has not been previously studied. On the basis of our previous work in hepatocytes showing that mTORC1 positively regulate VLDL production, we hypothesized that intestinal mTORC1 supports the production and secretion of chylomicrons. Lentivirus mediated shRNA technology was used to knockdown (&gt;90% down) mTORC1 signaling‐associated proteins, Raptor and TSC2, to create three Caco‐2 cell lines that exhibit basal (shScramble cells), low (shRaptor cells) or high (shTSC2 cells) mTORC1 activity. Chylomicron production was primed with the addition of 1.2 mM oleate/1.5% BSA complex to the apical side of ThinCert™ cell culture inserts. We found that knocking down Raptor disrupts triacylglycerol synthesis and chylomicron assembly. Knockdown of Raptor decreased the mRNA levels of APOB (−87%), MTTP (−94%), FASN (−76%), DGAT1 (−77%) and DGAT2 (−81%) compared with control shScramble cells. The cellular protein abundance of apoB (−89%), MTTP (−63%) and FASN (−80%), and the secretion of apoB100 (−51%), apoB48 (−66%) and triacylglycerols (−69%) were decreased compared with control shScramble cells. Rapamycin (a mTORC1 inhibitor) lowered the secretion of apoB100 and apoB48 in shScramble cells by 47% and 75%, respectively. Knockdown of Raptor decreased the gene expression of prechylomicron transport vesicle‐associated proteins VAMP7 (vesicle‐associated membrane protein 7) and SAR1B (secretion associated Ras related GTPase 1B) by 85% and 70%, respectively, compared with shScramble cells, suggesting that prechylomicron trafficking from the ER to the Golgi was repressed in the absence of Raptor. In conclusion, our studies revealed the critical role of mTORC1 in chylomicron metabolism. These findings have broad implications for the absorption of essential fatty acids and lipid‐soluble vitamins, for chylomicron retention disease, chylomicron overproduction and associated hypertriglyceridemia.Support or Funding InformationUSDA Hatch Act, USDA‐NIFA

PH-sensitive small molecule nanodrug self-assembled from amphiphilic vitamin B6-E analogue conjugate for targeted synergistic cancer therapy

To promote the targeted cancer therapy, the pH-sensitive small molecule nanodrug self-assembled from amphiphilic vitamin B6-E analogue conjugate was successfully constructed. Herein, water-soluble vitamin B6 with pKa (5.6) was chemically conjugated to lipid-soluble vitamin E succinate (α-TOS), which showed selective cancer cell killing ability and this amphiphilic small molecule vitamin conjugate could self-assemble to be free nanoparticles (NPs) and doxorubicin-loaded NPs (α-TOS-B6-NPs-DOX). The small molecule nanodrugs could perform the following characteristic: (i) stability in the sodium dodecyl sulfonate (SDS) solution and long-term storage stability in PBS via surface negative charge; (ii) tumor accumulation by enhanced penetration and retention (EPR) effect; (iii) improved cellular internalization by means of vitamin B6 transporting membrane carrier (VTC); and (iv) facilitating endosomal escape and rapid drug release for synergistic toxicity to tumor cells via charge reversal and ester hydrolysis at intracellular pH and/or esterase. Moreover, α-TOS-B6-NPs-DOX exhibited long blood circulation stability and significant tumor accumulation and inhibition with the decreased side effects in vivo. Thus, the pH-sensitive small molecule nanodrug self-assembled from amphiphilic vitamin B6-E analogue conjugate could be the potential drug carriers in targeted synergistic cancer therapy.

Investigating thermal and storage stability of vitamins in pasteurized mashed potatoes packed in barrier packaging films

Encapsulation may solve thermal and storage stability issues for vitamins in foods. Here, the role of encapsulation in improving thermal and oxidative stability of vitamin C (encapsulated (EVC) and non-encapsulated (NVC), lipid-soluble vitamins (A & E together, esterified forms) was determined in mashed potato. The product was vacuum packed in four different oxygen barrier films, pasteurized using a microwave-assisted system or conventionally, and then stored at 5 °C for 90 days. We observed 4–5 % losses of NVC, while no losses occurred in EVC, vitamin A & E after thermal processing. At the end of storage, NVC and EVC losses in mashed potato varied between 12–76 % and 13–31 %, respectively depending upon film type, while vitamin A & E remained stable. TPC and pH of mashed potato were stable over the storage period in all selected films, but the color changed significantly in the lowest barrier film. Processing affected the gas barrier, dielectric, and thermal properties of films to a variable extent. This study demonstrated the effectiveness of encapsulation in improving the stability of vitamin C and can help processors in selecting optimal packaging film.

Nutrient composition of underutilized skeins of flying fish (Hirundichthys oxycephalus): The new and better egg whites

Fish roes are consumed globally as a delicacy and an important seafood-based source of macro and micro-nutrients that is intensively produced in Indonesia. The connective material holding the roes i.e. the skeins are also high in protein and therefore could be utilized as nutritious protein supplement for food products. However, the study of the skeins is scant and their complete nutritional composition and physical appearance have not been fully described. We examined the nutritional composition and chemical resilience of fish roe skeins from the flying fish (Hirundichthys oxycephalus) that are commonly found in temperate and tropical oceans. The air-dried skeins contained 72–74 % crude protein, high-quality protein composed of essential and most non-essential amino acids, and very low carbohydrate content. High macro and micro minerals, lipids, albeit at a low amount, composed of saturated and unsaturated fatty acids and water- and lipid-soluble vitamins were also present. The roe skeins were highly durable and resistant to nutrient decomposition through over-desiccation as well bacterial contamination by Escherichia coli. In current production practices, the roe skeins are discarded as food waste. Our study proved that the roe skeins are rich in the nutrients that are valued from chicken egg whites, but provided additional essential macro- and micro-nutrients while being highly durable and resistant to bacterial contamination.

Vitamin D and Ovarian Cancer: Systematic Review of the Literature with a Focus on Molecular Mechanisms.

Vitamin D is a lipid soluble vitamin involved primarily in calcium metabolism. Epidemiologic evidence indicates that lower circulating vitamin D levels are associated with a higher risk of ovarian cancer and that vitamin D supplementation is associated with decreased cancer mortality. A vast amount of research exists on the possible molecular mechanisms through which vitamin D affects cancer cell proliferation, cancer progression, angiogenesis, and inflammation. We conducted a systematic review of the literature on the effects of vitamin D on ovarian cancer cell.

Zróżnicowane znaczenie witaminy E, jej występowanie oraz funkcje regulacyjne w różnych tkankach roślinnych

Fruits and vegetables contain vitamins, dietary fibre and phytochemicals, which promote human health. Specifically vitamins are major bioactive compounds. In plant raw materials there are water-soluble (vitamins B and C) and lipid-soluble (vitamins A, E and K) ones, having strong antioxidant potential and limiting several diseases, including cancer and cardio-vascular diseases. Furthermore, vitamins play a key role in plant protection against abiotic and biotic stress factors. Vitamin E is a group of eight fat-soluble compounds known as tocochromanols, which are synthesized exclusively by photosynthetic organisms. Tocochromanols are categorised into two groups – tocopherols and tocotrienols. Each group contains four forms identified by prefixes α-, β-, γ- and δ-, yielding a total of eight forms. Vitamin E is an essential, lipid-soluble antioxidant in the human diet, protecting from cancer, diabetes, cardiovascular and neurological diseases. This review summarizes the recent developments in the understanding the variety of roles of vitamin E as well as its major genetic regulatory pathways in plants. In addition, a comprehensive mapping of vitamin E occurrence is presented in different plant tissues, organelles and horticultural crop species, as well as throughout different stages of fruit development and after postharvest.

Histological evaluation of the ANTIOXIDANT effect of Vitamin E on reversing the negative impact of tartrazine on extraction socket healing. (Randomized controlled trial)

One of a synthetic food dye which used worldwide is Tartrazine, it is an orange-colored, water soluble with a potential toxicological risk. Food or drinks containing tartrazine caused sensitivity symptoms. Vitamin E is an essential lipid-soluble vitamin and has well-established antioxidant properties which improve the proliferation, differentiation, and mineralization, and reduced apoptosis of osteoblastic cells. Aim of this study: to evaluate the effect of tartrazine and co-administration of vitamin E on post extraction socket using histological examination by light microscope.Materials and methods: Surgical extraction of mandibular first molars were done in a twenty one albino rats, the animals were randomly divided into 3 groups; group I (control), group II given tartrazine and group III given tartrazine and Vitamin E (treated group). The duration of the experiments was 14 days. Histological evaluation was done by H & E and Masson trichrome.Results: the bone healing of the extraction socket in the Vitamin E treated group was nearly the same as the control group.Conclusion: Vitamin E has a protective effect as an effective antioxidant on bone healing in post extraction socket and may be useful as a supportive agent in an endless array of dental surgeries.

Vitamin E Supplementation and Mitochondria in Experimental and Functional Hyperthyroidism: A Mini-Review.

Mitochondria are both the main sites of production and the main target of reactive oxygen species (ROS). This can lead to mitochondrial dysfunction with harmful consequences for the cells and the whole organism, resulting in metabolic and neurodegenerative disorders such as type 2 diabetes, obesity, dementia, and aging. To protect themselves from ROS, mitochondria are equipped with an efficient antioxidant system, which includes low-molecular-mass molecules and enzymes able to scavenge ROS or repair the oxidative damage. In the mitochondrial membranes, a major role is played by the lipid-soluble antioxidant vitamin E, which reacts with the peroxyl radicals faster than the molecules of polyunsaturated fatty acids, and in doing so, protects membranes from excessive oxidative damage. In the present review, we summarize the available data concerning the capacity of vitamin E supplementation to protect mitochondria from oxidative damage in hyperthyroidism, a condition that leads to increased mitochondrial ROS production and oxidative damage. Vitamin E supplementation to hyperthyroid animals limits the thyroid hormone-induced increases in mitochondrial ROS and oxidative damage. Moreover, it prevents the reduction of the high functionality components of the mitochondrial population induced by hyperthyroidism, thus preserving cell function.

The study of chemical content and physicochemical properties of ostrich (Struthio camelus) fat (local)

The study contains the description of the ostrich fat by estimating the chemical content, which included moisture, protein, fat, ash, cholesterol, minerals and lipid-soluble vitamins, including vitamin (E andA) and the estimation of lipoproteins. Chemical properties were also studied which include peroxide value (PV), free fatty acids (FFA), saponification number (SN), iodine number and Physical properties include viscosity, density and specific gravity, refractive index, melting point and ester value (EV). Fatty acids were identified by using gas chromatography (GC). The results showed the percentage of moisture, protein, fat and ash were 5.56%, 5.16%, 87.88% and 1.03%, respectively. The mineral includes sodium, potassium, calcium, magnesium, phosphorus, iron and selenium reach to 8.5, 2.5, 40.11, 12.96, 78.6, 0.532 and 7.86 ppm respectively, cholesterol content was 50.63 mg / 100 g, vitamins content A and E was recorded 72.13 and 91.30 mg / ml respectively. The fat has good chemical and physical properties, such as viscosity, density and specific gravity, refractive index, melting point, saponification number, iodine number and ester value. While the results of lipoproteins in the ostrich fat HDL was 65 mg / dl, LDL 52 mg / dl and VLDL 35 mg / dl and triglycerides reach to 75 mg / dl. The peroxide number was 0.86 equivalent Meq O2 / Kg, and the percentage of the free fat acids reach to 0.38%. The fatty acid compassion was analyzed by gas chromatography (GC) and also showed nine saturated and unsaturated fatty acids including oleic acid, palmitic acid, arachidonic acid, erucic acid, heneicosanoic acid, undecanoic acid, lauric acid, caproic acid and myristic acid.

Effect of vitamin E sources and inclusion levels in diets on growth performance, meat quality, alpha-tocopherol retention, and intestinal inflammatory cytokine expression in broiler chickens

Water-miscible vitamin E (WVE) has been known to be more bioavailable than conventional lipid-soluble vitamin E (LVE) in human foods because of its greater solubility in the gastrointestinal tract. However, no data regarding the comparison of the efficacy of WVE to LVE were available in poultry diets. The objective of the current experiment was to investigate the effect of vitamin E (VE) sources and inclusion levels in diets on growth performance, meat quality, alpha-tocopherol retention, and intestinal inflammatory cytokine expression in broiler chickens. A total of 420 6-d-old broiler chicks were allotted to 1 of 7 dietary treatments with 6 replicates in a completely randomized design. The corn-soybean meal-based basal diet was formulated without inclusion of supplemental VE. Additional 6 diets were prepared by supplementing the basal diet with either WVE or LVE at the inclusion levels of 33, 65, or 100IU/kg in diets. Diets were fed to birds for 26 d. Results indicated that different VE sources and inclusion levels from 33 to 100IU/kg in diets had no effects on growth performance in broilers. The VE sources did not affect lipid oxidation in both breast and thigh meat. However, increasing inclusion levels of VE decreased (linear and quadratic, P < 0.05) lipid oxidation in breast meat. Regardless of VE sources, increasing inclusion levels of VE increased (linear, P < 0.01) alpha-tocopherol concentrations in breast meat. Dietary WVE showed a greater reduction in the expression of both pro- and anti-inflammatory cytokine genes in the jejunum than dietary LVE. Increasing inclusion levels of both VE sources decreased (linear and quadratic, P < 0.05) the expression of both pro- and anti-inflammatory cytokine genes in the jejunum. The efficacy of dietary WVE for growth performance, meat quality, lipid oxidation, and alpha-tocopherol retention in broilers chickens is similar to that of dietary LVE. However, it is likely that dietary WVE is more effective in decreasing intestinal inflammatory responses than dietary LVE in broilers.

The Lipide Soluble Vitamin Contents of Some Onobrychis Miller Taxa

The goal of this study is to determine the lipid-soluble vitamin contents in seeds of the some Onobrychis Miller taxa by using HPLC. Samples were collected from the natural habitats. Studied materials were dissolved in acetonitrile/methanol (75/25 v/v) and were injected 50 μL to HPLC instrument (Shimadzu, Kyota Japan). According to data obtained from present study showed that Onobrychis hypargyrea, Onobrychis viciifolia, Onobrychis caput-galli, Onobrychis fallax and Onobrychis oxyodonta var. armena have high lipide-soluble vitamin contents. Present study found that Onobrychis oxyodonta var. armena (1777,27±6,24 µg/g), Onobrychis fallax (916,0±4,51 µg/g) Onobrychis hypargyrea (809,7±5,03 µg/g) and Onobrychis viciifolia (399,7±3,54 µg/g) have highest beta-caroten content. Also, Onobrychis caput-galli has high beta caroten content (73,3±,94 µg/g). on the other hand, it was found that Onobrychis fallax has highest gamma-tocopherol content (1401,2±8,76 µg/g). Onobrychis viciifolia (574,9±2,35 µg/g), Onobrychis caput-galli (410,1±4,56 µg/g), Onobrychis oxyodonta var. armena (267,7±3,68 µg/g), Onobrychis podporea (162,5±2,14 µg/g) werethe other high gamma tocopherol content. Whereas, retinol, retinol acetate and r-tocopherol contents were found absent or trace amounts in the present study.

Long-chain vitamin K2 production in Lactococcus lactis is influenced by temperature, carbon source, aeration and mode of energy metabolism

BackgroundVitamin K2 (menaquinone, MK-n) is a lipid-soluble vitamin that functions as a carboxylase co-factor for maturation of proteins involved in many vital physiological processes in humans. Notably, long-chain vitamin K2 is produced by bacteria, including some species and strains belonging to the group of lactic acid bacteria (LAB) that play important roles in food fermentation processes. This study was performed to gain insights into the natural long-chain vitamin K2 production capacity of LAB and the factors influencing vitamin K2 production during cultivation, providing a basis for biotechnological production of vitamin K2 and in situ fortification of this vitamin in food products.ResultsWe observed that six selected Lactococcus lactis strains produced MK-5 to MK-10, with MK-8 and MK-9 as the major MK variant. Significant diversities between strains were observed in terms of specific concentrations and titres of vitamin K2. L. lactis ssp. cremoris MG1363 was selected for more detailed studies of the impact of selected carbon sources tested under different growth conditions [i.e. static fermentation (oxygen absent, heme absent); aerobic fermentation (oxygen present, heme absent) and aerobic respiration (oxygen present, heme present)] on vitamin K2 production in M17 media. Aerobic fermentation with fructose as a carbon source resulted in the highest specific concentration of vitamin K2: 3.7-fold increase compared to static fermentation with glucose, whereas aerobic respiration with trehalose resulted in the highest titre: 5.2-fold increase compared to static fermentation with glucose. When the same strain was applied to quark fermentation, we consistently observed that altered carbon source (fructose) and aerobic cultivation of the pre-culture resulted in efficient vitamin K2 fortification in the quark product.ConclusionsWith this study we demonstrate that certain LAB strains can be employed for efficient production of long-chain vitamin K2. Strain selection and optimisation of growth conditions offer a viable strategy towards natural vitamin K2 enrichment of fermented foods, and to improved biotechnological vitamin K2 production processes.

Vitamin E and Vitamin C Intake Among Lactating Mothers In Jakarta

Background and ObjectivesVitamin E is a lipid soluble vitamin which obtain only through diet. Vitamin E has many functions including antioxidant and well known to prevent lipid peroxidation in membrane cell. Vitamin C helps to regenerate vitamin E back into non radical. Vitamin E and C intake among vulnerable group such as lactating mother still uncleared especially in Jakarta. The aim of this study to determine level of vitamin E and C intake among lactating mothers in JakartaMethods60 lactating mothers aged 20–40 years old in 1–6 months postpartum were recruited on Grogol Petamburan and Cilincing Public Health Centre in Jakarta. This cross sectional study held from March 2019 until April 2019. Vitamin E and C dietary intake were collected with semi quantitative food frequency questionnaire. Descriptive analysis was used in this study.ResultsThis study showed that median value of vitamin E intake was 6.50 (1.20-43.10) gram/day with 91.7% subjects does not meet vitamin E recommended daily intake (RDA) recommendation (19 gram/day), otherwise 70% vitamin C intake was above RDA recommendation (100 gram/day) with median value is 120.05 (23.0-479.2) gram/day.ConclusionVitamin E intake among lactating mothers in Jakarta was not adequate and far below RDA recommendation but vitamin C intake among lactating mothers in Jakarta was adequate and met RDA recommendation.