Lipid Peroxidation Resistance Research Articles

Thermal stability, antioxidant activity and bioavailability of pea protein–naringin Pickering emulsion for enhanced delivery applications

After successfully addressing to mitigate bitterness of naringin through construction Pickering emulsion using pea protein (PP) and naringin (NG) in our previous study, we now probed thermal stability, antioxidant efficacy, and bioavailability. FTIR analysis and UV–vis spectroscopy indicated predominant interactions between PP and NG were hydrogen and hydrophobic bonds. TGA and DSC analyses demonstrated that PP-NG complexes exhibited superior heat-resistance compared to pure PP and NG. Thermal stability assessments indicated a significant retention of NG in the PP-NG Pickering emulsion than the control NG across varied temperatures (4 °C, 25 °C, 37 °C, and 65 °C). Moreover, the antioxidant activity of PP-NG emulsion was dependent on the concentration of NG, as evidenced by DPPH and ABTS free radicals scavenging abilities, ferric reducing power, and lipid peroxidation resistance. Additionally, PP-NG Pickering emulsion exhibited substantially high bioavailability (92.01 ± 3.91%). These results suggest a promising avenue for the application of NG with improved characteristics.

Berberine Hydrochloride-loaded Liposomes Gel: Preparation, Characterization and Antioxidant Activity

Abstract: Aim: Berberine hydrochloride is a Traditional Chinese Medicine component with antibacterial, antiviral, anti-inflammatory, antioxidant and lowering blood sugar effects. However, the application of berberine hydrochloride has been hindered for a long time due to its poor solubility and low bioavailability. This study aims to design a liposomes-gel to enhance the bioavailability and antioxidant activity of berberine hydrochloride. Materials and Methods: The thin-film dispersion hydration method was used to prepare liposomes, and liposomes-gel was prepared by the natural swelling method using sodium alginate as the hydrogel matrix. Berberine hydrochloride-loaded liposomes-gel was characterized by the encapsulation efficiency, particle size, potential, and transmission electron microscopy, and studied by in vitro release and antioxidant activity of the preparations. Results: The results revealed the encapsulation efficiency of berberine hydrochloride-loaded liposomes was 79.62±4.20% and the mean particle size was 153.7±11.2 nm. The release of berberine hydrochloride from liposomes-gel conformed to a first-order release model. The scavenging rate of DPPH free radicals and H2O2 by berberine hydrochloride-loaded liposomes-gel exceeds 70%. Conclusion: The liposomes-gel has a sustained-release effect on berberine hydrochloride. Berberine hydrochloride-loaded liposomes-gel hin vitroad good antioxidant properties by measuring the scavenging ability on DPPH free radicals, H2O2 and lipid peroxidation resistance. Keywords: Berberine hydrochloride, Liposomes, Sodium alginate, Hydrogel, Antioxidant.

Phenolic Antioxidants of Auricularia Species and Their Inhibitory Effects on α-Amylase, α-Glucosidase and Acetylcholine Esterase Activities

Auricularia is the genus of an edible jelly fungus known for its high nutritional value and good taste. In this study, the phenolic contents and in vitro bioactivities of four Auricularia species were investigated. The total phenolic contents ranged from 3.5 to 20.1 μmol gallic acid equivalents/g. Gallic acid, chlorogenic acid and rutin were the predominant phenolic acids present in the soluble fractions of the four species of Auricularia. Particularly, we identified gallic acid and rutin in A. cornea, gallic acid in A. heimuer and selenium-rich A. cornea var. Li, and chlorogenic acid in A. cornea var. Li. In addition, selenium-rich A. cornea var. Li. showed antioxidant properties including inhibitory activity against ABTS, free radical scavenging capacity and lipid peroxidation resistance. The selenium-rich A. cornea var. Li showed superior inhibitory activities toward α-amylase and acetylcholine esterase (IC50 311.7 and 388.6 μg/mL, respectively) compared to other three Auricularia cultivars. The A. cornea var. Li (IC50, 271.4 μg/mL) displayed strong inhibition towards α-glucosidase. The results suggest that a submerged culture of Auricula is an effective method to produce total phenolics which could potentially be used as a natural antioxidant in the food and pharmaceutical industries.

Chinese medicinal formula, Qinggan Huoxue Recipe protects rats from alcoholic liver disease via the lipopolysaccharide-Kupffer cell signal conduction pathway

The Chinese medicinal formula, Qinggan (QG) Huoxue (HX) Recipe (R) exerts a range of pharmacological effects, including reversible steatosis, decreased levels of inflammatory cytokines and lipid peroxidation resistance. The aim of the present study was to determine the specific mechanisms of QGHXR hepatoprotection through the lipopolysaccharide-Kupffer cell (LPS-KC) signal conduction pathway in rats with alcoholic liver disease (ALD). ALD rats were exposed to the compound factors, QGR and HXR. Hematoxylin and eosin staining was conducted to evaluate the pathological changes in the liver following QGHXR treatment and an enzyme-linked immunosorbent assay was performed to measure the content of tumor necrosis factor (TNF)-α in the plasma. Immunohistochemical staining was conducted to examine the expression of cell differentiation antigen (CD) 68 and 14. In addition, western blot analysis and reverse transcription-polymerase chain reaction were used to measure the expression of Toll-like receptor 4 (TLR4), phosphorylated-extracellular regulated protein kinases (p-ERK), nuclear factor (NF)-κB, CD14 and TNF-α. Following stimulation with the compound factors, the rats exhibited increased alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, as well as marked pathological changes. Furthermore, the related molecules in the LPS-KC pathway were upregulated and QGHXR was identified to be effective in the LPS-KC signal conduction pathway in the ALD rats. QGHXR was superior to QGR and HXR in reducing the serum ALT and AST levels, regulating CD14, TLR4, NF-κB, ERK and TNF-α as well as improving the pathological changes. The results indicated that QGHXR therapy may provide a novel strategy for treating ALD via regulation of the related molecules in the LPS-KC signaling pathway.

Ascorbate and urate are the strongest determinants of plasma antioxidative capacity and serum lipid resistance to oxidation in Finnish men

Copper-induced plasma lipoprotein oxidation resistance has usually been determined in separated low density lipoprotein (LDL) fractions, that do not contain water-soluble antioxidants present in blood plasma. The aim of this study was to find the main determinants of the measurements of copper-induced lipid oxidation resistance (lag time) in whole serum and plasma total peroxyl radical trapping capacity (TRAP) in a population sample of smoking ( n=25) or non-smoking ( n=26) middle aged men at high risk of cardiovascular diseases. Smokers had significantly lower plasma ascorbic acid values, but only slightly lower α-tocopherol, β-carotene and serum urate values than non-smokers. Plasma ascorbic acid concentration explained 23.5% of the lag time variation (standardized regression coefficient β=0.48; P=0.004) in smokers and 5.6% in non-smokers. Serum urate concentration was the strongest determinant of lag time in non-smokers ( β=0.64, P<0.001). In addition, serum albumin, lipid standardized α-tocopherol and serum high density lipoprotein (HDL) cholesterol entered the multivariate regression model for lag time. For plasma TRAP, only urate and ascorbic acid entered the multivariate regression model. Lag times in serum and in isolated very low density lipoprotein (VLDL) and LDL fraction did not correlate, but the maximal rate of these reactions correlated significantly. These results confirm that lipid peroxidation resistance in serum or plasma are associated with ascorbic acid, urate, α-tocopherol, albumin and HDL concentrations. The measurement of lipid oxidation resistance in whole serum might be more physiological than in isolated lipoprotein fraction, as the effects of water-soluble antioxidants are not artificially removed.