Lipid Oxidation In Meat Products Research Articles

Sodium nitrite (NaNO2) regulating myoglobin-mediated lipid oxidation in washed bovine mince

ABSTRACT This study investigated the effect of NaNO2 on inhibiting myoglobin-mediated lipid oxidation in muscle using a washed bovine mince model. We measured various parameters, including TBARS and PV to determine lipid oxidation, non-heme iron release, protoporphyrin destruction, and nitrosylmyoglobin production. The results of the study showed that the addition of NaNO2 during myoglobin-mediated lipid oxidation significantly reduced the fading and lipid oxidation of washed muscle (p < 0.05). Additionally, the addition of NaNO2 reduced the release of non-heme iron and the degradation of protoporphyrin in myoglobin. Nitrosylmyoglobin was formed in the washed mince supplemented with NaNO2 and myoglobin. These findings suggest that NaNO2 can effectively inhibit the lipid oxidation process induced by myoglobin, which could be a crucial aspect of nitrite inhibition of lipid oxidation in cured meat products. Overall, the study provides evidence for the potential use of NaNO2 as a food preservative to prevent lipid oxidation in meat products.

Evaluation of effects of ultrasound-assisted curing on the flavor of Chinese bacon

The curing stage is of great importance in flavor formation during Chinese bacon processing. Ultrasound-assisted curing plays an essential role in the Lipid oxidation of meat products. In this study, GC–MS and electronic nose were used to analyze the influence of different power ultrasonic-assisted curing on the flavor formation of Chinese bacon. Through the analysis of phospholipid and lipase, the fundamental precursors of ultrasonic on the flavor of Chinese bacon were determined. It was found that there were differences in the flavor contour description of Chinese bacon between the ultrasonic treatment group, mainly due to the change in the W1W sensor. A total of 28 volatile compounds were detected by GC–MS, and the aldehyde content increased with ultrasonic power. PC and PE are the main flavor precursors in the curing process. This study provides a theoretical basis for improving the curing technology of Chinese bacon.

Development of a Novel Steam Distillation TBA Test for the Determination of Lipid Oxidation in Meat Products.

The 2-thiobarbituric acid (TBA) method has been used for the spectrophotometric determination of secondary lipid oxidation products, such as malonaldehyde (MA), due to its good correlation with sensorial perception of lipid oxidation. Other approaches have been proposed over time. Direct distillation can result in artificially increased MA concentrations due to intense heating. Extraction is a milder and faster method, but it suffers from false color development in the presence of sugars or other compounds. A novel approach using steam distillation for the recovery of MA was developed. Validation and optimization studies were conducted, aiming to maximize MA recovery from various meat product samples by adjusting the steam distillation parameters. For the optimal MA recovery, 10 g of the sample, 25 mL of H2O, and 5 mL of 2 N HCl were used. The sample was distilled using a stream of water vapor until 50 mL of the distillate was collected in less than 3 min. Subsequently, 5 mL of the distillate was reacted with 5 mL of 0.02 M TBA, and the absorbance was measured at 532 nm. MA recovery was 61.8%. Experimentation with varying nitrite levels suggested that the addition of sulfanilamide is necessary when NaNO2 is more than 50 mg MA/kg. The proposed method is fast, milder than direct distillation, and eliminates the issue of TBA interacting with sugars and other compounds.

Enhancing the physicochemical performance of myofibrillar gels using Pickering emulsion fillers: Rheology, microstructure and stability

Water loss and lipid oxidation of meat products during processing and storage compromise their sensory qualities, such as flavor, tenderness, juiciness. Herein, surfactant-stabilized emulsions and Pickering emulsions were employed as particulate fillers and fat substitutes in myofibrillar protein-based gels (MPGs). Relatively weak MPGs were formed with the addition of NaCl, which were further enhanced via filling with soy protein isolate nanoparticle-stabilized Pickering emulsions (SNPE) or soy protein isolate nanoparticle-stabilized Pickering emulsions with cinnamaldehyde (SNPE-CA). After thermal treatment at 80 °C, incorporation of SNPE-CA contributed to a highly compact gel network and the highest gel strength, but the minimum moisture content. SNPE resulted in a moderately compact network and the maximum moisture content, MPGs without fillers possessed a looser network and similar gel strength with those containing SNPE. While Tween 80 hindered the aggregation of myofibrillar proteins, causing the presence of Tween 80-stabilized emulsions (TWE) in MPGs generated the loosest network and the lowest gel strength. After further centrifugation or cooking, MPGs with SNPE showed the highest water holding stability, TWE conduced to the moisture stability of MPGs against centrifugation, MPGs with TWE or SNPE-CA or without fillers had a similar water holding stability index during cooking at 100 °C. Additionally, the ability of the three emulsion fillers for improving lipid oxidation stability of MPGs decreased in the following order: SNPE-CA > SNPE > TWE. These findings indicate that Pickering emulsions are potential particulate fillers to enhance moisture stability in meat gels, as well as fat substitutes to protect lipid from oxidation.

Inhibition of lipid oxidation and hexanal production in cooked meats by microbial iron chelator deferriferrichrysin from rice wine

Lipid oxidation imparting off-flavor and decreasing the shelf life of meat is a significant concern of the global food industry. Here, the efficacy of a natural iron chelator, deferriferrichrysin (Dfcy), contained in sake (Japanese rice wine), against lipid oxidation of meat was investigated. Purified Dfcy and Dfcy-enhanced sake were prepared and added to three kinds of meat (chicken, pork, and beef) and heated at 70°C for 70 min, followed by storage at 4°C for 10 days and analysis of the efficacy to inhibit lipid oxidation. Dfcy reduced thiobarbituric acid-reactive substances (TBARS) values of meat products by over 50% with the addition of 100 mg/kg of Dfcy. Hexanal production was more efficiently reduced, that is, over 90% reduction with the addition of 50–100 mg/kg of Dfcy. The findings suggest Dfcy and Dfcy-containing sake as promising novel natural antioxidants to inhibit lipid oxidation in meat products. Novelty Impact Statement This study investigated the natural iron chelator deferriferrichrysin (Dfcy) contained in sake (Japanese rice wine) against the lipid oxidation of meat products. Purified Dfcy and Dfcy-containing sake inhibited hexanal production with over 90% reduction at 50–100 ppm in chicken, pork, and beef meat. Dfcy was shown to be an efficient inhibitor of lipid oxidation in meat products, and the strategy used in this study could be used to develop schemes for the production of other rice wines that contain iron chelator.

Advances and gaps in studies on healthy meat products and their relationship with regulations: The Brazilian scenario

BackgroundStrategies for the reformulation of meat products respond to consumers’ demand for healthier food alternatives. The reduction of salt or sodium and animal fat, and the substitution of synthetic antioxidants by natural ones have been a subject of study for Brazilian researchers for many years, but the industrial application of new technological alternatives presents the challenge of adapting to regulations related to the processing of meat products. Scope and approachReports by Brazilian researchers from 2011 to 2019 were collected from the Scopus database and their abstracts were submitted to a textual analysis to visualize the trend of studies over these years. The relationship between the approaches/ingredients proposed in the studies and the Brazilian regulation was discussed. Key findings and conclusionsNumerous alternatives for natural antioxidants and salt and animal fat substitutes have been proposed, but Brazilian regulation is restrictive with respect to the use of natural antioxidants and the most studied salt substitute in meat products. However, studies using alternatives more compatible with Brazilian regulations have been proposed. For industrial application purposes, some flavorings and spices may have antioxidant activity and act as natural antioxidants, delaying the lipid oxidation of meat products; the sodium content can be reduced using several morphologies or sizes of salt particles; and the incorporation of oils rich in polyunsaturated fatty acids, embedded in oleogels and microparticles using encapsulating proteins, seem to be a viable alternative for reducing animal fat in meat products.

Evaluation of Antioxidant Activity of Cudrania tricuspidata (CT) Leaves, Fruit Powder and CT Fruit in Pork Patties during Storage

The objective of this study was to assess antioxidant activities of leaves and fruit powder of Cudrania tricuspidata (CT) with different particle sizes (crude, 500 μm, 150 μm), and determine the physicochemical properties and microbial counts of pork patties with various levels of CT fruit powder (CTFP) during refrigerated storage. Total phenolic content of crude leaves had the highest value of 3.54 g/100 g (p<0.05). Overall, CT leaves (CTLP) had higher total phenolic content than CTFP (p<0.05). 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of CTFP was higher than that of CTLP (p<0.05), which showed higher iron chelating ability and reducing power than CTFP (p<0.05). After pork patties were manufactured with 0.5% and 1.0% of CTFP at 500 μm, pH, color values, thiobarbituric acid reactive substance (TBARS), and peroxide values (POV) were then measured. The addition of CTFP into pork patties significantly (p<0.05) increased redness and yellowness values of patties. TBARS values of pork patties containing CTFP were lower (p<0.05) than those of CTFP-0 patties after 10 days of storage. Pork patties added with CTFP showed no significant (p>0.05) difference TBARS values among different storage periods. POV values of pork patties containing CTFP were lower than those of the control from 3 days up to the end of refrigerated storage (p<0.05). These results suggest that CTFP could be used as a natural antioxidant to retard lipid oxidation in meat products during refrigerated storage.

Bilberry and Sea Buckthorn Leaves and Their Subcritical Water Extracts Prevent Lipid Oxidation in Meat Products.

The aim of this study was to find new sustainable, Nordic natural antioxidant sources, develop subcritical water extraction (SWE) process for recovering the antioxidant compounds from the most potential raw materials, and to test their antioxidative effects in meat products. The antioxidant capacities of water and 50% ethanol (aq) extracts of 13 berry, grain, and horticultural plant materials as well as hexane/ethanol extracted stilbene fractions from pine heartwood and spruce inner bark were measured in hydrophilic and lipophilic systems. Tree, bilberry leaf (BL), and sea buckthorn leaf (SBL) extracts showed the highest antioxidant capacities. BL and SBL were selected for the development of SWE. The optimal conditions for recovering maximal antioxidative capacities were 110 °C/1 min for SBL and 120 °C/1 min for BL. Dried BL and SBL and the respective optimized subcritical water extracts were applied in chicken slices and pork sausage, and their ability to prevent lipid oxidation was evaluated during 8 and 20 days storage, respectively, at 6 °C. All tested plant ingredients effectively prevented lipid oxidation in the products compared to the control samples. Sensory acceptance of the plant ingredients was good, especially in the chicken product. To our knowledge, this is the first study to assess the antioxidant effects of SW extracted berry leaves in meat products.

Relationship between the antioxidant capacity of soy sauces and its impact on lipid oxidation of beef patties

The aim of this study was to determine the relationship between in vitro antioxidant capacity of soy sauces and its impact on lipid oxidation in raw and cooked beef patties during 10 days of refrigerated storage. Three commercial soy sauces were used: industrially fermented soy sauce (IS), traditionally fermented Korean soy sauce (KS), and mixed soy sauce (MS). In vitro antioxidant capacity, KS showed the highest total phenol content, whereas IS and MS had a higher Fe2+ chelating activity than KS (P < .05). Heat treatment decreased total phenol content but increased Fe2+ chelating activity of soy sauces (P < .001). In both raw and cooked beef patties, the addition of IS or MS caused a lower 2-thiobarbituric acid reactive substances (TBARS) level than KS throughout the overall storage period (P < .05). This preliminary study suggests that the Fe2+ chelating activity of soy sauce may be one of the major mechanisms in preventing lipid oxidation in meat products.

Potential application of corn starch edible films with spice essential oils for the shelf life extension of red meat.

To investigate the effect of corn starch (CS) edible films with spice oils on the stability of raw beef during refrigerated storage. The antimicrobial properties of corn starch films containing 0-4·0% (w/v) ratios of clove (CL) and cinnamon (CI) essential oils (EOs) were tested against seven meat spoilage organisms by zone inhibition test. Effects of CS films containing 3% CL or CI or a mixture of CL+CI were also tested in raw beef stored at 4°C. Meat samples were analysed for pH, microbial counts, colour values and Thiobarbituric acid reactive substances (TBARS) values for a period of 15days. CS films with CL+CI resulted in a significant reduction in microbial populations in the meat samples and also improved meat colour stability at the end of storage period. The incorporation of spice EOs in CS films may provide antimicrobial and antioxidant activities that could improve the stability of raw meat. Results from this study may be applied in meat industries as an additional barrier to control microbial spoilage as well as lipid oxidation in meat products.

Effects of calcium soap of rice bran oil fatty acids supplementation alone and with DL-α-tocopherol acetate in lamb diets on performance, digestibility, ruminal parameters and meat quality.

Thirty-six Malpura lambs (28day old and 6.7±0.25kg BW) were distributed equally in three groups having six males and six female. They were adlibitum fed individually three different experimental diets containing calcium soap of fatty acids (CA-FA) at 0 (T1 ) and 40 (T2 and T3 ) g/kg concentrate up to six months of age. Animals in T3 were supplemented additionally with 40mg DL-α-tocopherol acetate/kg of concentrate. The roughage moiety included adlibitum dry Prosopis cineraria and fresh Azadirachata indica leaves. All the lambs were allowed to suckle from their dam up to weaning (90day of age). Supplementation of Ca-FA improved weight gain and feed conversion ratio during both pre- (28-90days) and post-weaning (91-180days) phases; however, no effect of DL-α-tocopherol was observed. Metabolic parameters during post-weaning phase revealed non-significant effect on digestibility but improved nitrogen balance in the test groups. The effect on biochemical attributes did not show any significant alteration in ruminal parameters, blood biochemicals and urinary purine derivatives. Carcass traits revealed higher (p<0.05) dressing yield and loin eye area with Ca-FA supplementation. The value of thiobarbituric reactive substances for nuggets prepared from frozen carcasses revealed significant (p<0.05) reduction in T3 compared to the other dietary groups. Fatty acid profile of adipose tissue revealed higher (p<0.001) 9-octadecanoic, 9-12-octadecadienoic, polyunsaturated fatty acids (PUFA), higher ratio of PUFA/saturated fatty acids (SFA), ω-6/ω-3 and lower SFA in Ca-FA-supplemented groups. It is concluded that supplementation of 40g/kg calcium soap prepared from industrial grade rice bran oil in lamb ration provided additional energy intake, improved N utilization, gain and feed conversion ratio besides improving dressing yield and meat quality with CLA enriched fatty acid profile. DL-α-tocopherol acetate when supplemented at 40mg/kg feed reduced lipid oxidation of meat products thus improving its keeping quality.

Effect of Various Herbal Medicine Extracts on the Physico-chemical Properties of Emulsion-type Pork Sausage

The purpose of this study was to evaluate the functional properties of herbal medicine aqueous extracts as natural preservatives to improve the quality characteristics and oxidative stability of emulsion-type pork sausage. The pH value was significantly higher in sausages of control than in groups containing herbal medicine extracts (p<0.05). The shear force values increased with the addition of herbal medicine extracts (p<0.05). The addition of herbal medicine extracts also reduced the lightness and redness, and increased the yellowness, chroma, and hue observed during storage (p<0.05). In addition, the anti-oxidative activity including 2-thiobarbituric acid reactive substances (TBARS), peroxide value (POV), and 2,2-diphenyl-1-picryhydrazla hydrate (DPPH) was higher in the Akebiaquinata (T3), Lonicera japonica (T4), and Chelidoniummajus (T5) groups than in the other groups (p<0.05). In a sensory evaluation, the Liriopeplatyphylla (T1) and Saposhnikoviaedivaricata (T2) groups scored lower than the control, whereas the T3, T4, and T5 groups showed similar attributes compared with the control. In conclusion, the addition of herbal medicine extracts did not have negative effects on the quality of emulsion-type pork sausage, and they could be used as a natural antioxidant material to prevent lipid oxidation in meat products.

Effect of ethanolic flax (Linum usitatissimum L.) extracts on lipid oxidation and changes in nutritive value of frozen-stored meat products.

Flaxseed (Linum usitatissimum L.) is an important source of phenolic compounds, mainly lignans. Antioxidant capacities of flaxseed extracts that contain the compounds have been reported earlier. However, there is a lack of accessible information about their activity against lipid oxidation in meat products. Therefore, the effect of ethanolic flaxseed extracts (EFEs) on lipid stability and changes in nutritive value of frozen-stored meat products (pork meatballs and burgers) was determined. EFEs from three Polish flax varieties (Szafir, Oliwin, Jantarol) were applied in the study. During 150-day storage of meat products, the lipid oxidation (peroxide and TBARS value) and thiamine retention were periodically monitored, alongside with methionine and lysine availability and protein digestibility. The addition of EFEs significantly limited lipid oxidation in stored meatballs and burgers. EFE from brown seeds of Szafir var. was superior to the others from golden seeds of Jantarol and Oliwin. Moreover, the extracts reduced changes in thiamine and available lysine content, as well as protein digestibility, during storage time. The effect of EFE addition on available methionine retention was limited. The ethanolic flaxseed extracts exhibit antioxidant activity during frozen storage of meat products. They can be utilized to prolong shelf-life of the products by protecting them against lipid oxidation and deterioration of their nutritional quality. However, antioxidant efficiency of the extracts seems to depend on chemical composition of raw material (flax variety). Further investigations should be carried on to explain the issue.

Impact of high pressure treatment and intramuscular fat content on colour changes and protein and lipid oxidation in sliced and vacuum-packaged Iberian dry-cured ham

The effect of high hydrostatic pressure (HHP) (600MPa) and intramuscular fat content (IMF) on colour parameters and oxidative stability of lipids and proteins in sliced vacuum-packaged Iberian dry-cured ham during refrigerated storage (120days at 2°C) was investigated. Several studies have investigated the influence of HHP on lipid oxidation of meat products. However, its effects on protein carbonylation, as also the influence of IMF content on this carbonylation are poorly understood. HHP treatment had a significant effect on lean lightness after 0 and 120days of storage while IMF content increased lightness and yellowness over time. Regarding oxidative stability, the effect of HHP treatment depended on IMF content samples with a high IMF having greater lipid instability while samples with a low IMF underwent more protein carbonylation.

First evaluation of unfermented and fermented rooibos (Aspalathus linearis) in preventing lipid oxidation in meat products

This study consisted of two trials aiming to evaluate, for the first time, the antioxidant potential of rooibos in meat products. With this purpose, the first trial evaluated three unfermented (green) rooibos forms (dried leaves, water extract, freeze-dried extract) added at 2% inclusion level to ostrich meat patties on an 8-day shelf-life trial. A Control group without green rooibos inclusion was also considered. The second trial evaluated the addition of different concentrations (0%, 0.25%, 0.5% and 1%) of a fermented rooibos extract to nitrite-free ostrich salami. The 2% green rooibos inclusion considerably lowered the TBARS content of ostrich patties, in this way extending their shelf-life. The fermented form (0.5% and 1%) was also effective in delaying lipid oxidation in ostrich salami until 15 days of ripening. The antioxidant potential of both green and fermented forms of rooibos in meat products was confirmed, even if its effect on lipid oxidation requires further study and long-term effects are not yet fully understood.

A critical examination of some experimental variables in the 2-thiobarbituric acid (TBA) test for lipid oxidation in meat products

By comparison of various procedures for the determination of TBARS (2-thiobarbituric-acid-reactive substances) in non-cured meat samples, it was found that a direct extraction procedure with trichloroacetic acid gave significantly higher reaction yields than a rapid Kjeltec steam distillation procedure (50 ml distillate in 2.5 min). When optimizing the distillation procedure with regard to distillation principle (heating by thermal conductivity or steam injection) and rate, the reaction yield was more affected by changes in distillation principle than by changes in distillation rate. Simple distillation (conduction heating) yielded significantly higher TBA values than steam distillation at the same distillation rate. The reproducibility of the distillation procedure was found to be about 5–10 times poorer than of the extraction procedure and, because of the high reaction yields and robustness of the extraction procedure, the use of the extraction procedure is recommended with food matrices such as non-cured meat samples with less than 14–18% fat and no artificial colorants.

Catalysts of lipid oxidation in meat products

In emulsions consisting of refined lard, egg white and corn starch haemoglobin, initially a mixture of the oxy and met forms, at levels similar to the haemoprotein contents found in fresh meat, was a far more powerful catalyst of lipid oxidation, as measured by TBA number, than inorganic iron compounds at levels appropriate to those found in meat. This was true over the pH range 4·5 to 7·5. When added and evenly distributed to exhaustively washed muscle fibres (WF), at levels appropriate to those found in meat, haemoglobin was again a powerful catalyst, but all forms of inorganic iron appeared to have little prooxidant activity. The rate of oxidation of the lipid was very dependent on the haemoprotein concentration, being maximal in the range 10 −4 to 10 −5 M. This equates to an approximate unsaturated lipid to haem molar ratio of several hundred to one, similar to the values reported for model linoleate haem systems. In heated systems the haemoprotein again appeared to be a more effective catalyst than inorganic iron at levels appropriate to those found in meat. It is concluded that the conflicting results as to the roles of haem pigments and inorganic iron in lipid oxidation found in the literature are due, at least in part, to the difficulty of evenly dispersing the catalysts in the washed fibres, especially if heat or freezing leads to subsequent phase separation, and that H 2O 2, formed by autoxidation of the oxypigments, may be necessary for ferric haem pigments to be effective catalysts.