Lipase-related Protein Research Articles

In silico analysis of Cucumis pubescens Willd. fruit extract phytocompounds and its activity against anti-diabetic targets

Diabetes mellitus is a chronic disease which causes complications in a large population worldwide. Traditionally, medicinal plants possess numerous bioactive compounds to treat chronic diseases. In this study, the bioactive compounds in Cucumis pubescens Willd. were screened computationally to treat diabetes. Ten ligand molecules from C. pubescens Willd. plant fruit were selected from GC-MS analysis. The target proteins like Lipase-related Protein 2 (2OXE), α – amylase (2QV4) and β – Glucosidase (2ZOX) were retrieved from the PDB databank. The proteins have been selected based on their role in anti-diabetic activity. All ten ligands were docked with all three proteins to identify the suitable ligand possessing high binding energy. The ligand (4H-Pyran-4-one, 2,3-Dihydro 3,5-Dihydroxy-6-Methyl) had a maximum binding energy of -4.3 kcal/mol and -4.4 kcal/mol with the targets 2OXE and 2ZOX respectively. The ligand molecule 1,4-dimethyl-2,3-diazabicyclo[2.2.1]hept-2-ene produced the highest binding energy of -4.7 kcal/mol with 2QV4. The predicted active phytochemicals can be used as natural drug molecules to treat diabetes mellitus in future.

Type 1 Hyperlipoproteinemia and Recurrent Acute Pancreatitis due to Lipoprotein Lipase Antibody in a Young Girl with Sjögren's Syndrome

Type 1 hyperlipoproteinemia (T1HLP) in childhood is most often due to genetic deficiency of lipoprotein lipase (LPL) or other related proteins. The aim was to report a case of marked hypertriglyceridemia and recurrent acute pancreatitis due to the presence of LPL autoantibody in a young girl who was subsequently diagnosed with Sjögren's syndrome. A 9-yr-old African-American girl presented with acute pancreatitis and serum triglycerides of 4784 mg/dl. Strict restriction of dietary fat reduced serum triglycerides, but she continued to experience recurrent pancreatitis. Approximately 18 months thereafter, she developed transient pauciarticular arthritis with elevated serum antinuclear antibody (>1:1280). Minor salivary gland biopsy revealed chronic sialadenitis with a dense periductal lymphocytic aggregate suggestive of Sjögren's syndrome. Genomic DNA was analyzed for LPL, GPIHBP1, APOA5, APOC2, and LMF1. Immunoblotting was performed to detect serum LPL autoantibody. The patient had no disease-causing variants in LPL, GPIHBP1, APOA5, APOC2, or LMF1. Immunoblotting revealed serum LPL antibody. The patient responded to immunosuppressive therapy for Sjögren's syndrome with resolution of hypertriglyceridemia. Unexplained T1HLP in childhood could be secondary to LPL deficiency induced by autoantibodies. Therefore, diagnosis of autoimmune T1HLP should be entertained if clinical features are suggestive of an autoimmune process.

Proteimics of hyperlipidemia-associated pancreatitis using differential gel electrophoresis and tandem mass spectrometry: experiment with rats

Objective To analyze the injury mechanism of hyperlipidemia-associated acute pancreatitis utilizing proteomics. Methods Ten SD rats were fed with high fat feed to establish hyperlipidemic models, and 10 SD rats were fed with normal feed to be used as control group. Six weeks later 5 rats from each group were intraperitoneally injected with caendin to establish acute edematous pancreatitis models, and 5 rats from each group were injected with bile acid taurocholate into pancreatic duct to cause acute necrotizing pancreatitis. Nine hours after the establishment of models the rats were killed with their pancreases taken out. Differential protein analysis was performed using two-dimensional differential ingel electrophoresis (2D-DIGE) and tandem mass spectrometry (MALDI-TOF/TOF). Immunohistochemical method was adopted to confirm the significantly changed proteins. Results Rat models of hyperlidemiaassociated acute edematous pancreatitis and rat models of hyperlidemia-associated acute necrotic pancreatitis were well established. Fifty-nine spots were detected by DeCyder Analysis Software between acute necrotic pancreatitis with and without hyperlipidemia samples and 39 markedly changed protein spots were identified by MALDI-TOF-TOF. Twelve spots were detected between the samples of acute edematous pancreatitis with and without hyperlipidemia and 7 of them were identified. Differentially expressed proteins in hyperlipidemiaassociated pancreatitis included pancreatic enzymes, such as lipase, amylase, and alpha-l-antiproteinase,ER stress and calcium influx related proteins including GRP78, HSP60 and protein disulfide isomerase.Other proteins associated with DNA replication and damage repair, apoptosis, cell metabolism, circulatory dysfunction, and signal transduction were identified in hyperlipidemia-associated acute pancreatitis.Conclusion Some proteins related to ER stress, apoptosis, circulatory dysfunction and signal transduction show differential expression in acute pancreatitis with hyperlipidemia. Hyperlipidemia intensifies acute pancreatitis through various ways. ER stress-related proteins such as GRP78, HSP60, protein disulfide isomerase, and calreticulin can be regarded as biomarkers of early diagnosis and target of further treatment in severe acute pancreatitis. Key words: Pancreatitis, acute necrotizing; Hyperlipidemias ; Electrophoresis, gel, twodimensional; Endoplasmic reticulum

High expression in adult horse of PLRP2 displaying a low phospholipase activity

The physiological role of the two lipase-related proteins, PLRP1 and PLRP2, still remains obscure although some propositions have been made concerning PLRP2. In this paper, we report the presence of high amounts of PLRP2 in adult horse pancreas whereas no PLRP1 could be detected. As well, a non-parallel expression of PLRP2 and PLRP1 is observed in adult cat and dog, since no PLRP2 could be detected in these two species. In adult ox, neither PLRP2 nor PLRP1 could be found. These findings are in favor of a different regulation of the expression of the genes encoding pancreatic lipase and the related proteins according to the species. The cDNA encoding horse PLRP2 has been cloned and the protein expressed in insect cells. Both native and recombinant PLRP2 display the same catalytic properties. They possess a moderate lipase activity, inhibited by bile salts and not restored by colipase. Interestingly, they differ from PLRP2 from other species by their very low phospholipase activity indicating that PLRP2 could not be considered as a general phospholipase as previously postulated. This work highlights the variability of the properties of PLRP2 and rises the question of the physiological function of this protein in adult according to the species.