Lines Hs Research Articles

MOLECULAR ASSESSMENT OF GENETIC DIVERGENCE AMONG MAIZE GENOTYPES

Research carried out in 2021 at the Jeser Al-Musayyab Company, Baghdad, Iraq, determined the molecular variations among 10 inbred lines of maize (Zea mays L.), for use in the future production of promising F1 hybrids. DNA sequencing random polymorphism technology using Inter Simple Sequence Repeats (ISSR) with five primers (UBC 834, UBC 835, UBC 856, UBC 866, and UBC 897) assessed the genetic variations among the 10 inbred lines of maize. All five primers produced 29 bands, averaging 5.8 strips each primer. The four primers, UBC 834, UBC 835, UBC 866, and UBC 897, had produced the same and highest number of proliferators with molecular weights ranging from 100 to 2000 bp. The primer UBC 856 showed the lowest number, with a molecular weight of 100–500 bp. The total number of polymorphs was 24, with a percentage of 82.4%, with the lowest (80%) polymorphs recorded in the primer UBC 856. The least discriminatory efficiency and ability values were 17.24% and 16.66%, respectively. Relying on binary data and genetic divergence using the UPGMA method to create a genetic kinship tree, the maize lines were separated into two groups, i.e., A, B, secondary, and sub-secondary groups. The highest genetic similarity (with the lowest genetic divergence) was estimated according to the Nei and Li coefficient of 0.23 between the two lines HS and S-10. The presented findings can provide further guidance and help analyze the genetic diversity among the maize populations.

Genetic Diversity Among Selected Japanese Quail (Coturnix coturnix japonica) Lines Using RAPD Markers

Karabağ, K. and Balcioğlu, M.S. 2010. Genetic diversity among selected Japanese quail (Coturnix coturnix japonica) lines using RAPD markers. J. Appl. Anim. Res., 38: 149–152. The intra and inter genetic polymorphisms and genetic relatedness of 12 generations selected 3 lines (HBW1, LBW, L); 7 generations selected 1 line (HBW2) and a control (C) line in Japanese quail were investigated by RAPD-PCR. Fourteen DNA samples (7♂-7 ♀) from each line were analyzed using 24 primers. One hundred ninety six polymorphic loci were amplified (150 to 2600 bp) and polymorphism rate was calculated as 99.49%. Genotypie polymorphism rates were calculated as 63.45%, 31.47%, 42.13%, 35.53% and 36.55% in C, LBW, HBW1, HBW2 and L, respectively. Average heterozygosis within lines (Hs), total heterozygosis (HT)and average Shannon index (I) values were 0.169, 0.354 and 0.528, respectively. The genetic variation was highest in C and lowest in LBW line. The genetic identity within line varied from 0.747 (C) to 0.875 (DCA) and between lines varied from 0.603 (C-L) to 0.813 (HBW1-LBW). It was found that genetically similar selection line to base ancestor (C) is HBW2 line (0.667) while farthest is L line (0.494). Thus genetic variation within line and the genetic relationships among lines could be estimated successfully using RAPD-PCR in selected Japanese quail lines.