Objective: Hop tests have well-known benefits when evaluating the rehabilitation process after sports injuries. However, utilization of hop tests to evaluate functional athletic performance of healthy athletes is currently uncertain and is not common in clinical practice. This study aimed to investigate the relationships between hop performance, their symmetries and functional test performance in American Football (AmF) players. Materials and Methods: Twenty-six collegiate AmF players aged 20.9±2.5 years participated in this study. According to their playing positions, players categorized as Skill Players (SP), Big Skill Players (BSP) and Linemen (LM). Body Mass Index (BMI) and body fat ratio were measured. Then, subjects were performed hop tests and 10-yards Lower Extremity Functional Test (LEFT). Players were also analysed by dividing into low and high performers for LEFT, according to their LEFT test duration. Results: Hop test performance of the SP was superior to LM for SLH, TH, and CH. The performance of BSP was inferior to SP for CH, and was superior to LM for SLH. In LEFT, the SP group was significantly faster than LM. For LEFT, TH, CH and 6H test results were significantly different between low and high performer players. Conclusion: Hop tests may be utilized to evaluate the functional athletic performance of AmF players. Physical characteristics and playing positions should also be considered. Symmetry had no effect on LEFT.