Lime Oil Research Articles

Microemulsion containing Kaffir lime oil using photo-crosslinkable bio-based copolymer

The research aimed to prepare a microemulsion of Kaffir lime oil (KLO) using photocrosslinkable bio-based copolymers as polymer shells. First, cinnamyl alcohol was used as a biomonomer, which was functionalized with methacrylic anhydride via an esterification reaction to contain a double bond named cinnamyl methacrylate. Then, it was copolymerized with methacrylic acid by solution iodine transfer polymerization, resulting in polymethacrylic acid-block-polycinnamyl methacrylate-block-poly methacrylic acid (PMAA-b-PCMA-b-PMAA) with a molecular weight of 2700 g/mol and a chain length of PMAA-b-PCMA-b-PMAA equals 7: 6: 7 units. After that, PMAA-b-PCMA-b-PMAA (BioSurf) was used as the polymer shell to encapsulate KLO in a microemulsion system cooperative with Tween 80 (surfactant; Surf) and ethanol (co-surfactant; CoSurf). The ratio of kaffir lime oil: Surf-CoSurf was varied from 1: 9 to 9: 1 (wt) where Surfmix was BioSurf: Surf: Co-Surf ratio of 1: 1: 1 (wt) dispersed in water at 400 rpm for 5 min. The optimal contents of oil, Surfmix, and water were 6 %, 24 %, and 70 %, respectively, with the nanocapsule size at about 68 nm. After UV curing at 254 nm for 120 min, the dimerization of cinnamyl groups in PCMA was confirmed by UV spectroscopy, where the absorbance peaks of cinnamyl groups at 283 and 294 nm significantly decreased. The encapsulated KLO was slowly permeating skin which was only 10 % after 1 h, with the anti-inflammatory percentage of inhibited nitric oxide production in macrophage cells at 19.24 ± 1.63. Moreover, nanocapsules did not show toxicity to human skin cells at concentrations less than or equal to 1 v/v%. Moreover, the microemulsion is thermodynamically stable at 4 °C, 30 °C, and 45 °C. The encapsulation of KLO in microemulsion might be an alternative candidate for cosmetic products and related applications.

Strength of Kaolinitic Clay Soil Stabilized with Lime and Palm Oil Fuel Ash

The use of palm oil fuel ash (POFA) mixed with lime as a catalyst in soil stabilization can significantly improve the stability of problematic soils and improve their engineering properties. Problematic soils can obstruct the construction process due to its low strength and low bearing capacity. In this study, various laboratory tests were carried out to determine the engineering properties of the soil’s mixture which includes Atterberg limit, particle size distribution, compaction, and unconfined compression test. 4%, 8% and 12% POFA were mixed with 6% hydrated lime to stabilized the kaolinitic clay soil at different curing days (1, 7, 14, and 30 days). Compared to untreated kaolin, the addition of POFA plus lime resulted in higher undrained shear strength. The maximum undrained shear strength (USS) is 32.68kN/m2, which was obtained on the 30th day of curing with the optimal mixture of stabilized kaolin which is kaolin mixed 6% of lime and 12% of POFA. The unconfined compressive strength increased by 185.04% compared to the unconfined compressive strength of untreated kaolinitic clay with a value of 65.36 kN/m2. This proves that kaolin stabilized with lime and POFA can increase the strength parameters of clay, thus reducing construction costs for soil stabilization and reducing environmental issues.

Shelf-life extension of Thai green papaya salad dressing by hurdle technology

Green papaya salad or Som Tum is the most popular spicy mixed salads in Thailand due to its unique rich flavor. Green papaya salad dressing (GPSD) is made from various ingredients such as fresh chili pepper, fresh garlic, rind tamarind, fish sauce and lime oil, including the limitation in controlling the taste and flavor of salad dressing and its poor shelf-life. In this study, a convenient ready-to-eat GPSD was developed. Hurdle technology was applied to extend shelf-life of the GPSD based on monitoring of microbial contamination and food pathogens throughout the process. Hurdle technology able to decrease total plate count (TPC) from 5.6 ± 0.2 to 1 ± 0.3 log CFU/g and yeast and mold (Y&M) from 4.2 ± 0.3 to <1 log CFU/g. After 12 weeks of storage at 5 ± 2 °C, slightly increase of TPC was detected as 1.5 ± 0.2 log CFU/g and no changes were found for Y&M and other pathogens. At week 12, GPSD stored at 32 ± 2 °C was found to have higher TPC and Y&M (3.7 ± 0.3 and 2.4 ± 0.3 log CFU/g, respectively). Therefore, a combination of hurdles that combines low aw, low pH, heat treatment, low temperature after hot filling, and chilled storage could extend the shelf-life of GPSD with satisfy sensorial test result and be suitable for minimally processed salad dressing.

Sensory Impact of Novel Dihydrocoumarins in Native Lime Oils.

Citrus fruits have been known and valued for their aroma in food and perfume ever since humans have maintained written records. Often described as the "champagne" of citrus oils, especially cold pressed lime peel oils have raised attention. Particularly peel oils of Citrus latifolia exhibit a pleasant coumarinic, sweet, and balsamic aroma in comparison to its close relative, the Citrus aurantifolia. Those coumarinic notes have not been completely understood until today. Thus, this study aimed to identify the responsible substances and elucidate their contribution and impact on the aroma of cold-pressed lime oil. By combining distillation, fractionation, olfactory detection, and structure elucidation, the responsible key aroma components were identified. A combination of coumarins and their corresponding saturated analogs have been identified to significantly contribute to the typical coumarinic-like aroma, including three new flavor compounds that have not yet been described in the literature as lime oil constituents: 7-methoxy-2-chromanone (3,4-dihydro-7-methoxy-2H-1-benzopyran-2-one; CAS 20921-02-2), 5,7-dimethoxy-2-chromanone (3,4-dihydro-5,7-dimethoxy-2H-1-benzopyran-2-one; CAS 82243-01-4) and 5,6-dihydrobergaptene (5,6-dihydro-4-methoxy-7H-furo[3,2-g][1]benzopyran-7-one; CAS 29050-61-1). The sensorial evaluation of the impact of these components on the lime aroma profile has shown flavor-modulating effects and the ability to enhance aldehydic-peely, juicy, and fruity notes as well as their importance in reproducing the authentic, typical coumarin-like notes.

Citrus Essential Oils as Potential Insecticides Against Thrips flavus

Background: Essential oils (EOs) are recognized for their potent insecticidal activity and are widely considered promising agents for pest control. Methods: The insecticidal activity of seven commercial citrus EOs against the global phytophagous pest Thrips flavus was evaluated using bioassays conducted under laboratory conditions. Moreover, the chemical composition of these EOs was analyzed using gas chromatography-mass spectrometry (GC-MS). Results: Among the 45 identified compounds, the major constituents were d-limonene (23.77%– 95.10%), methyl jasmonate (38.76%), and linalyl acetate (34.55%). Orange flower oil, sweet orange oil, tangerine peel oil, bergamot oil, lime oil, lemon oil, and grapefruit oil exhibited good insecticidal activity against T. flavus without demonstrating significant repellent activity. Orange flower oil and sweet orange oil showed higher insecticidal toxicity, with LC50 values of 0.20 g/L and 0.37 g/L, respectively. In pot experiments, the higher control efficacy against T. flavus was found after 7 days of treatment for orange flower oil (91.14±1.27% at 720.00 g a.i.·hm−2 and 100% at 900.00 g a.i.·hm−2), sweet orange oil (91.14±5.52% at 900.00 g a.i.·hm−2), tangerine peel oil (96.20±2.19% at 720 g a.i.·hm−2 and 100% at 900.00 g a.i.·hm−2), lemon oil (96.20±2.19% at 900 g a.i.·hm−2), and lime oil (97.47±2.53% at 900.00 g a.i.·hm−2). Conclusion: Taken together, orange flower oil and tangerine peel oil, which were found to contain d-limonene and methyl jasmonate, demonstrated rapid and more effective insecticidal activity compared to the other EOs tested, which makes these two EOs promising alternatives to chemical insecticides.

Chemical composition and antibacterial activity of essential oil kaffir lime (Citrus hystrix DC) leaves from East Borneo

Kaffir lime (Citrus hystrix DC) leaves are a versatile plant with high commercialization potential. In the pharmaceutical industry, kaffir lime leaf essential oil is used to stimulate appetite, as a cosmetic ingredient, and as an antibacterial agent. Although most studies have concentrated on the essential oil of kaffir lime leaves, its components and biological activities need to be considered based on the growing location. The chemical components and biological activity of kaffir lime leaf essential oil are affected by differences in growth location. This research was conducted to determine the chemical composition and antibacterial activity of the essential oil of kaffir lime leaf essential oil from East Borneo. Fresh and dried kaffir lime leaves were extracted by steam distillation method and chemical compound analysis by Gas Chromatography-Mass Spectrometry (GC-MS) and the antibacterial activity of essential oils was analyzed by disc diffusion method. There are twenty-five compounds in the essential oil of fresh and dried kaffir lime leaves. The main components of fresh kaffir lime leave essential oil were citronellal (77.29%), linalool (4.88%), sabinene (4.83%), citronellol (3.41%), β-myrcene (1.23%), and citronellal (74.09%), sabinene (5.54%), linalool (3.59%), citronellol (3.56%), caryophyllene (2.26%) were the main components of the essential oil of dried kaffir lime leaves. The antibacterial activity of essential oils against Staphylococcus aureus ATCC 25925 and Escherichia coli ATCC 8733, with zones of inhibition of 21.75 and 19.17 mm, respectively. The minimum inhibitory concentration on all antibacterial activities was 6.25 μL/mL. The presence of strong antibacterial activity in dried and fresh kaffir lime leaf plant extracts was associated with the presence of citronellal compounds found in essential oil kaffir lime leaves

Application of lime peel oil composite nanoemulsion to prevent toxigenic fungi in nuts

Food byproduct oils may have antimicrobial impacts when used in coating and preservation. Nuts are known to suffer from toxigenic fungi and their related mycotoxins. The present study utilized lime oil emulsion to minimize fungal infection and reduce aflatoxin B1 (AFB1). Besides, it evaluated lime oil's impact on nuts' protection against oxidation and deterioration during storage. Lime oil was extracted using hydrodistillation, and gas chromatography (GC-MS) evaluated volatile constituents. Oil was loaded into a composite emulsion of whey protein, Arabic gum, gelatin, and carboxymethyl cellulose. The antimicrobial and antifungal properties of the nut-coating emulsion were evaluated. A simulated Aspergillus flavus infection experiment evaluated composite resistance for fungal infection and AFB1 production. Oxidation and acidity changes in nuts oil composition were evaluated by proximate analysis, fatty acid composition, and induction period. The oil majority was recorded for terpenes and monoterpenes, including limonene (44.69 ± 2.11%). The emulsion was characterized by zeta potential (−21.16 ± 1.28 mV), stability (99.61 ± 0.02%), and polydispersity index (0.41 ± 0.05). Antimicrobial properties recorded a high antibacterial inhibition zone (up to 28.37 ± 0.11 mm) and anti-mycotoxigenic fungi (up to 37.61 ± 0.24 mm). For the simulated experiment, fungal growth reduction ranged between 78.02% for filmed-peanut and 84.5% for filmed-almond, while AFB1 was not detected in filmed hazelnut and almond. During the one-year storage of samples, there was a slight change in nut oil composition and oxidation progress in filmed nuts, while there was a significant change in non-filmed nuts. The result recommended lime-composite as an edible nut coating that prevents aflatoxigenic contamination, oxidation changes, and improved shelf life.

Atsiri Oil Potential of Peel of Kaffir Lime, Lime, and Calamansi Orange as Antioxidant and Antibacteria

Orange is a member of the Rutaceae family which is known as one of the horticultural crops that is widely cultivated in the tropical and subtropical regions of southern Asia. Citrus plants such as fruit peels are used as a source of essential oil-producing ingredients that have antioxidant and antimicrobial activity. This study aims to determine the potential of kaffir lime, lime, and calamansi orange peel essential oils as antioxidants and antibacterials. Scoping review uses the PEOS framework and several databases such as the electronic database Atlantis Press, PubMed, ScienceDirect, and the Google Scholar search engine. Search results that meet the criteria are then analyzed using PRISMA Flowchart, Data Extraction, and Theme Mapping. The results of the scoping review study of 74 articles related to titles and abstracts, 8 articles met the inclusion and exclusion criteria. Essential oils from kaffir lime peel (Citrus hystrix), lime (Citrus aurantifolia), and calamansi orange (Citrus microcarpa Bunge.) have antioxidant and antibacterial activities. The higher the concentration of essential oils from the peel of the fruit, the greater the antioxidant and antibacterial activity produced. Fresh orange peel essential oil has the strongest antioxidant and antibacterial activity compared to orange twig and leaf oil.

Exogenous trans-cinnamic acid applications affect leaf, flower and peel essential oils of Banzhair lime cultivated on arid region

Exogenous trans-cinnamic acid applications affect leaf, flower and peel essential oils of Banzhair lime cultivated on arid region

Comparison of in vitro Killing Effect of Thai Herbal Essential Oils, Tea Tree Oil, and Metronidazole 0.75% versus Ivermectin 1% on Demodex folliculorum.

Abnormal proliferation of Demodex mites causes a skin disorder called demodicosis and has been linked to rosacea. The development of alternative therapy against Demodex mites is currently required. The ability to kill Demodex mites of Thai herbal essential oils has never been explored. This study aimed to study and compare the in vitro killing effect of Thai herbal essential oils, tea tree oil, and metronidazole 0.75% with ivermectin 1% on D. folliculorum. D. folliculorum mites were collected from the wastes of diagnostic standardized skin surface biopsy samples of demodicosis and rosacea patients for the trial. The microscopic evaluation started immediately after the mites were exposed to immersion oil (negative control), Thai herbal essential oils, tea tree oil, metronidazole 0.75%, and ivermectin 1% (positive control). The survival times of ten mites from each test agent were compared. The efficacy of Thai herbal essential oils and other test agents can be arranged in order as follows: lemongrass oil > sweet basil oil > clove oil > tea tree oil > lesser galangal oil > ginger oil, kaffir lime oil, peppermint oil > citronella oil > galangal oil > cajeput oil > ivermectin 1% > metronidazole 0.75%. This current study demonstrated the in vitro killing efficacy on D. folliculorum: Thai herbal essential oils, Tea tree oil > ivermectin 1% > metronidazole 0.75%. Thai herbal essential oils have the potential to be an adjuvant or alternative therapy against Demodex mites. Further in vivo studies are necessary to determine the treatment efficacy and side effects.

Isoimperatorin therapeutic effect against aluminum induced neurotoxicity in albino mice.

Background: Although aluminum (Al) is not biologically crucial to the human body, classical studies have demonstrated that excessive human exposure to Al can induce oxidative damage, neuroinflammatory conditions and neurotoxic manifestations implicated in Alzheimer's disease (AD). Exposure to Al was reported to be associated with oxidative damage, neuroinflammation, and to enhance progressive multiregional neurodegeneration in animal models. Several plant-derived natural biomolecules have been recently used to reduce the toxic effects of Al through decreasing the oxidative stress and the associated diseases. A good candidate still to be tested is an active natural furanocoumarin, the isoimperatorin (IMP) that can be extracted from Lemon and lime oils and other plants. Here, we examined the neuroprotective effects of IMP on aluminum chloride (AlCl3)-induced neurotoxicity in albino mice. Methods: Twenty-four male albino mice were used in this study. Mice were randomly devided into 5 groups. The first group was given distilled water as a control, the second group was given AlCl3 orally (10mg/wt/day) starting from the 2nd week to the end of the 6th week, the third group received AlCl3 orally and IMP interperitoneally, i. p. (30mg/wt/day) starting from week 2 till week 6 where IMP was supplement 1st and then 4h later AlCl3 was given to mice. The fourth group received the control (IMP 30mg/wt, i. p.) from the 2nd week till the end of the experiment. Rodent models of central nervous system (CNS) disorders were assessed using object location memory and Y-maze tests in 6th week began. Essential anti-inflammatory and oxidative stress indicators were evaluated, including interleukin-1 β (IL-1β), tumor necrosis factor α (TNF-α), malondialdehyde (MDA), total antioxidant capacity (TAC), and catalase activity (CAT). In addition, serum levels of brain neurotransmitters such as corticosterone, acetylcholine (ACh), dopamine and serotonin in brain homogenates were measured calorimetrically. Results: The study results revealed that the daily treatment of AlCl3 upregulated the TNF-α and IL-1β levels, increased MDA accumulation, and decreased TAC and CAT activity. In addition, aluminum induced a reduction in concentrations of ACh, serotonin and dopamine in the brain. However, IMP significantly ameliorates the effect of AlCl3 through modulating the antioxidant and regulating the inflammatory response through targeting Nrf2 (NF-E2-related factor 2) and mitogen-activated protein kinase (MAPK). Conclusion: Thus, IMP might be a promising treatment option for neurotoxicity and neurodegenerative diseases, such as Alzheimer's disease and Parkinson's disease, which are associated with neuro-inflammation and oxidative stress.

Essential Oil-Added Chitosan/Gelatin-Based Active Packaging Film: A Comparative Study

Active packaging films based on chitosan/gelatin were prepared using a solution casting method by adding various essential oils (lime, tea tree, rosemary, and thyme essential oils), and their effects were compared. The fabricated films were characterized and various physical properties as well as the antioxidant performance of the films were studied. Adding essential oils to the polysaccharide/protein biopolymer mixtures resulted in compatible films with high transparency (&gt;90% transparency). The mechanical strength and stiffness of the chitosan/gelatin films were improved by about 30% in the presence of essential oil, but the flexibility slightly decreased, and the stiffness improved. On the other hand, the water vapor barrier properties, thermal stability, and hydrophobicity of the essential oil-containing films were not significantly changed. Adding various essential oils significantly enhanced the antioxidant activity of chitosan/gelatin-based films. Therefore, bio-based functional films with added essential oils can be applied in active packaging applications.

Aktivitas Antibakteri Minyak Atsiri Kulit Jeruk Limau (Citrus amblycarpa (Hassk.) Ochse) Dalam Mengendalikan Bakteri Streptococcus mutans

Dental caries is an infection disease of tooth tissue caused by Streptococcus mutans bacteria. Atsiri is essential oil is a liquid-shaped compound that is thought to have potential as an antibacterial. The purpose of this study was to determine the antibacterial potential of essential oil of limau lime in controlling Streptococcus mutans bacteria. The antibacterial test in this study used disk disfussion method with 8 treatment groups and 4 replications is 5 concentrations of atsiri oils, 1 positif disc amoxicillin antibiotic, 2 negative control with DMSO and aquades. The data obtained were analyzed with normality test, Anova test and BNT test. Isolation of atsiri’s oil of limau lime in this study using the method of steam-water distillation. &#x0D; Streptococcus mutans bacteria were isolated from dental caries patients and tested using macroscopic, microscopic, gram staining, and biochemical methods. The study found that the atsiri oils of limau lime has antibacterial activity in vitro against Streptococcus mutans bacteria, as evidenced by the formation of an inhibit zone. Concentration as 100% with 15.38mm inhibitory zone diameter is the most effective concentration to inhibit the growth of Streptococcus mutans bacteria. The statistical analysis revealed a significant influence between the treatments at the 5% significance level.

Biological effect of alcohol extracts for oils of Cypress Cupressus sempervirens L. in the life performance of southern cowpea beetle Callosobruchus maculatus F. (Coleoptera:Chrysomelidae).

The effect of alcohol extracts twigs and cones (male and female) Cypress lime oils on different phases of southern cowpea beetle Callosobruchus maculatus F. (Coleoptera:Chrysomelidae) was carried out. The results showed the efficiency of both oils with significant effects on immature stages and adult emergence. &#x0D; The Cypress cones extract oil was more effective than twigs oil extracts in activity in all treatments numbers. The rates of productivity and first generation (F1) reduction reached 34.96% and 87.22% respectively at 1.5% concentration compare with Cypress twigs oil extracts which reached 66.22% and 81.58% respectively. The all oil concentrations that used in this study did not all effect on the germination rates ranged between 80.00- 90.00% The superiority of the alcoholic extract oil cones cypress lime to reduce the percentage of the damage amounted to 31.11% as compared to alcohol oil extracts of its twigs and control treatment, which amounted to 44.66% and 99.66 - 99.33%, respectively and found significant differences in the ratios in the attraction and repellency responses. The Cypress twigs oil in different concentrations showed a superior repellency effect on insect. The highest average of individuals repellency reached 7.00 and 8.00 rates 70.00 and 80.00% with attractant rates13.30 and 20.30 % as it balance rates reached -33.00 and -60.00% after 15 and 30 minutes of treating reached maximum at 1.5%, while Cypress cones oil showed significant attractant effect, the concentration 1.5% recorded highest rates of attractant, the average of individuals attractant reached 5.00 and 7.66% rates 50.00 and 76.60% and repellent rates 13.30 and 16.60% as it balance rates reached + 36.67 and + 60.00 % after 15 and 30 minutes of treating .

Lime and orange essential oils and d-limonene as a potential COVID-19 inhibitor: Computational, in chemico, and cytotoxicity analysis

The COVID-19 pandemic has substantially impacted the world health systems, causing public health concerns, and the search for new compounds with antiviral activity is of extreme interest. Natural molecules with bioactive potential are a trend, with essential oils (Eos) being the focus of recent studies. Thus, this study evaluates in chemico the d-limonene inhibitory activities in the viral genome of SARS-CoV-2 and analyzes the cytotoxic potential and safety profile of d-limonene and lime and orange EOs with a high content of d-limonene. The EOs were extracted and characterized, and the in chemico computational analysis for the determination as a potential anti-SARS-CoV-2 was performed with d-limonene, the major compound in EOs. The cytotoxicity analysis of EOs and d-limonene was carried out with MRC-5 and HaCaT, and the preliminary safety profile was also evaluated by the HET-CAM assay. d-limonene was suggested as a promising compound for anti-SARS-CoV-2 research, since the molecule does not provide mutagenic and cytotoxic fragments, and does not have irritating potential when diluted, in addition to having favorable pharmacokinetic characteristics, through in chemico analysis. Collectively, the results reveal the antiviral potential of lime and orange EOs, as well as their major compound. In this sense, further studies should be conducted to understand the antiviral mechanisms.

Effect of Steam Distillation Time on Antimicrobial Activity of Kaffir Lime Hydrosol to Gram-Positive and Gram-Negative Bacteria

This study was conducted in order to investigate the antimicrobial activity of kaffir lime hydrosol produced from steam distillation at different times to gram-positive and gram-negative bacteria. The study measured the antimicrobial activity of kaffir lime hydrosol produced from steam distillation at different times against S. aureus (gram-positive) and E. coli (gram-negative) bacteria. Hydrosols were obtained from steam distillation time of 4, 6 and 8 hours. Antimicrobial activity was represented by the clear zone in the disk-diffusion method. The clear zone on the paper disk indicates the diameter of the bacterial inhibition zone. Testing for the strength of inhibition was done using paper disks. The largest diameter of the zone of inhibition was found in the hydrosol of kaffir lime oil obtained from 6 hours of distillation time, amounting to 0.783 cm in gram-positive bacteria (S. aureus) and 0.73 cm in gram-negative bacteria (E. coli). Hydrosol of kaffir lime oil with variation in distillation times proved resistant to gram-positive and gram-negative bacteria.

Intelligent grading of kaffir lime oil quality using non-linear support vector machine

&lt;span lang="EN-US"&gt;This paper presents kaffir lime oil quality grading using the intelligent system classification method, a non-linear support vector machine (NSVM). This method classifies the quality kaffir lime oil into two groups: high and low quality, based on their significant chemical compounds. The 90 data of kaffir lime oil were used in this project from high to low quality. The abundance (%) of significant chemical compounds will act as the input and high or low quality as an output. The 90 data will be divided into two sets: training and testing data sets with a ratio of 8:2. The radial basis function (RBF) optimization kernel parameters in NSVM. Using the implementation of MATLAB software version R2020a, all data and analysis work was performed automatically. The results showed that the NSVM model met all performance criteria for 100% accuracy, sensitivity, specificity, and precision.&lt;/span&gt;

Essential Oils as Potential Source of Anti-dandruff Agents: A Review.

Dandruff is a frequent occurring scalp problem that causes significant discomfort to approximately 50% population at some stage of life, especially post-puberty and preadult age. In this review, we aim to summarise the recent findings regarding anti-fungal properties of herbal essential oils against pathogens involved in dandruff prognosis. A literature search of studies published between 2000 and 2020 was conducted over databases: PubMed, Google Scholar, Scopus, and Science Direct. Literature was explored using the guidelines given in Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). Dandruff, characterised by clinical symptoms of dryness, pruritis, scaly, and flaky scalp, is considered as a primary manifestation of seborrheic dermatitis. Amongst various etiological and pathophysiological factors, significant role of yeasts, primarily, species of Malassezia, Candida, has been strongly correlated with dandruff, while incidences of M. furfur, M. restricta and M. globosa are high compared to others. Due to relapse of symptoms with withdrawal of conventional anti-dandruff products, patients find best alternatives in natural products. Essential oils of herbal origin such as tea tree oil, lime oil, rosemary oil, have gained global importance in dermatology. These oils are rich in aromatic secondary metabolites, especially terpenes and phenolic components that impart substantial antimicrobial properties and resisting biofilm production. On the basis of the available information, we can conclude that essential oils have huge potential to be developed as anti-dandruff products, however, further studies are warranted to establish their efficacy in dandruff cure.

Kombinasi Minyak Kayu Putih dan Jeruk Nipis terhadap Mortalitas Pediculus humanus capitis

The use of synthetic chemical pediculicides is known to have side effects and the occurrence of resistance so plant-based pediculicides are needed to kill Ph. capitis. Eucalyptus and lime oil are known to have an effect in killing Ph. capitis. The purpose of this study was to determine the effectiveness of the combination of eucalyptus and lime oil on the mortality of Ph. capitis. This study was conducted in a true experimental with a post-test-only control group design in May 2021. This study consisted of 5 treatment groups, one group as a positive control (permethrin 1%), one group as a negative control (aquadest), and 3 groups received treatment with a combination of eucalyptus and lime oil in the proportions of 1:3 (25 ml of eucalyptus oil: 75 ml of lime juice), 1:1 (50 ml of eucalyptus oil: 50 ml of lime juice) and 3: 1 (75 ml eucalyptus oil: 25 ml lime juice). It was repeated 3 times so that the total sample was 150 Ph. capitis which was observed every 5 minutes for 1 hour. The research data were analyzed using the Kruskal-Wallis test and continued with the Posthoc Mann-Whitney U test. Mortality of Ph. capitis in the combination group the most effective was the combination of 75% eucalyptus oil and 25% lime oil in the 5th-minute observation of 100% mortality compared to the control group and other groups.

Moisture Sorption Isotherm of Cassava Starch Film Incorporated with Kaffir Lime Oil

The use of bioplastic film has been gaining a lot of interest especially in food packaging, however knowledge on its stability during handling is limited. Moisture sorption isotherm is a study that determines the stability of the film when exposed to certain environmental conditions. The gravimetric method was used to determine the moisture sorption isotherm of cassava starch film incorporated with kaffir lime essential oil (EO) at 30°C and 40°C. The moisture content increases with increasing water activity for both 30°C and 40°C. The sorption data were fitted into 3 models and Oswin was found to be the best model to fit the data with R2 of 0.93 to 0.97. The sorption data were used to determine the net isosteric heat of sorption (qst) through the application of Clausius – Clapeyron equation which provides an approximation of the minimum energy required to remove water from a solid. Generally, the qst decreases exponentially with an increase in moisture content regardless of whether the film contains EO. The incorporation of EO within these temperatures does not affect the moisture content and heat of sorption of the starch-based bioplastic film, which shows its stability and potential as an additive in food packaging film.