Light oil is a high-quality crude oil. The formation and chemical composition of light oil is a matter of great concern to petroleum geologists. Yakela Field and Shunbei Field, which are located in Yakela and Shunbei regions, respectively, are the two main light oil fields in the Tabei uplift of the Tarim Basin. In order to identify the causative mechanism of light oils in these two fields, 17 crude oil samples were selected and analyzed by gas chromatography (GC) for saturated hydrocarbons and gas chromatography–mass spectrometry (GCMS) for saturated and aromatic biomarkers. Then, the compounds were measured via the mass spectrometry detector after GC separation. The molecular geochemical characteristics showed that the light oils in the two fields are sourced from similar marine source rocks deposited under weak oxidative and reductive environments. The maturity of crude oil in the Shunbei Field is higher than that in the Yakela Field. Simulation results of hydrocarbon generation history of source rocks in two fields showed that the formation mechanism of the two light oils is different. The light oil in the Yakela Field is directly generated by the source rock in the late oil-generating window. The crude oil in the Shunbei Field is formed by the deep burial and maturation of the crude oil generated in the early stage of the source rock.
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