In light-frame timber construction, the shear wall is one of the most important components that provide resistance to lateral loads such as earthquakes or winds. According to KDS (Korea Design Standard) 42 50 10, shear walls are to be constructed using wood-based structural sheathing, with studs connected by 8d nails spaced 150 mm along the edge and 300 mm in the field. Even though small-scale residential timber building can be designed to exhibit seismic resistance using light-frame timber shear walls in accordance with KDS 42 50 10, only the abovementioned standard type of timber shear wall is available. Therefore, more types of timber shear walls composed of various materials should be tested to measure their seismic resistance, and the results should be incorporated into the future revision of KDS 42 50 10. In this study, the seismic resistance of shear walls composed of structural timber studs and wood-based structural sheathing with reinforced nailing is tested to evaluate the effects of the reinforcement. For the nailing reinforcement, shear wall specimens are constructed by applying nail spacings of 75–150 mm and 50–100 mm. For the shear wall specimens with one sheathing and reinforced nailing, the shear strengths are 1.7–2.0 times higher than that of the standard shear wall (nail spacing of 150–300 mm). The shear strength of the shear walls with sheathing on both sides is 2.0–2.7 times higher than that of the standard shear wall.