Aim: The acquisition of lifelong learning habits by healthcare professionals for the enhancement of their knowledge and skills post-graduation holds paramount significance for the beneficiaries of healthcare services. The aim of this study was to evaluate the lifelong learning approaches of emergency ambulance employees. Material and Methods: Within the scope of the research, data was collected online from 390 healthcare professionals working in emergency ambulances using the lifelong learning tendencies scale in in Turkey. When evaluating the data, group characteristics were summarized by descriptive analyses and In the comparisons of numerical variables, normal distribution condition was sought and nonparametric comparison tests were used. Results: Within the scope of the research, the mean lifelong learning approach scores of the health personnel working in the emergency ambulance were found to be 113.89±16.03 (min. 61, max. 145) and the obtained score shows that their lifelong learning tendencies are at a low level. The findings indicate that there is no statistically significant difference among groups based on gender, title, age, and years of work experience in terms of the total scores on the Lifelong Learning Tendencies Scale (p>0.05 for each). However, significant differences were observed among certain groups in terms of the subscales of the scale. It was determined that participants aged 30 and above exhibited decreased levels of motivation and perseverance. Additionally, it was found that as years of work experience increased, deficiencies in learning levels and curiosity deficiencies decreased. Conclusion: Emergency ambulance workers need to be prepared and willing to improve their theoretical and practical skills as new evidence emerges in their field, where scientific knowledge is increasing. According the research findings should focus on promoting self-directed and team-based learning, improving access to up-to-date information, and providing easily accessible educational resources.