Life Sciences Research Articles

Morphologic transformation of ferrofluid during micropump driving under field control.

The superiority of ferrofluid pumps in the fields of biomedical, life science, energy, and power research has been experimentally demonstrated. However, the mechanisms underlying the morphological transformations of ferrofluid fusion and separation during pump driving are not completely understood. To bridge the gap between the theory and practical applications of ferrofluid pumps, we employed optical methods to record the dynamic morphological transformation of rotating and fixed ferrofluids under different magnetic field polarities, magnetic field distributions, and ferrofluid mass fractions. The magnetic field polarity causes dynamic differences in the fusion-separation process of the ferrofluid but does not affect the volume segmentation of the ferrofluid, which depends on the ratio of the magnetic field intensities. When this ratio deviates from one, the morphology of ferrofluid changes, reducing the pumping efficiency. Compared to external environmental factors, the mass fraction does not change the morphology of the ferrofluid. However, high mass fractions lead to wall-clinging of the ferrofluid, and low mass fractions induce bubbles, both of which detrimentally affect the pumping performance. This study reveals the properties of ferrofluid and the effects of external environmental conditions on the morphological transformation of ferrofluid, providing references for optimizing ferrofluid pumps.

List jako świadectwo życia kulturalno-naukowego na przykładzie spuścizny rękopiśmiennej ks. Jana Kudery

Father Jan Kudera, together with Father Emil Szramek and Konstanty Prus, belonged to the first generation of researchers of the Silesian literary regionalism. This paper presents the results of an analysis the correspondence of Fr. Jan Kudera, kept at the Archdiocesan Archives in Katowice. The analysed documents come from 9 fonds of the legacy bearing reference numbers from ZS 515 to ZS 523. The research of a historical and literary character was conducted mainly through content analysis. The letter was treated as a document and at the same time an autonomous statement. Correspondence from the legacy of the priest can be a valuable and unique source of information on cultural and scientific life in Silesia in the years 1922–1939 and about Kudera itself.

Energy-resolved neutron imaging study of a Japanese sword signed by Bishu Osafune Norimitsu.

Our research focuses on elucidating the crystallographic structure of Japanese swords in a nondestructive manner using the neutron imaging instrument RADEN at the Materials and Life Science Experimental Facility of the Japan Proton Accelerator Research Complex(J-PARC). We developed an analysis method combining wavelength-resolved Bragg-edge imaging and wavelength-selective neutron tomography with a new strategy and applied it to an approximately 45-cm blade length Japanese sword signed by Bishu Osafune Norimitsu. Computed tomography was performed, and the three-dimensional analysis captured the characteristic internal structure of Kobuse. Kobuse is the most famous steel-combining structure of Japanese swords, where an outer steel with high-carbon content (Kawagane) covers a core steel with low-carbon content (Shingane). The crystallite size distribution obtained through Bragg-edge analysis could consistently explain the internal structure of two steels observed in neutron tomograms. Our nondestructive imaging revealed deep hardening, forming a wavy pattern more than 5mm from the cutting edge.

Functional Organic Electrochemical Transistor-Based Biosensors for Biomedical Applications

Organic electrochemical transistors (OECTs), as an emerging device for the development of novel biosensors, have attracted more and more attention in recent years, demonstrating their promising prospects and commercial potential. Functional OECTs have been widely applied in the field of biosensors due to their decisive advantages, such as high transconductance, easy functionalization, and high integration capability. Therefore, this review aims to provide a comprehensive summary of the most recent advances in the application of functional OECT-based biosensors in biomedicine, especially focusing on those biosensors for the detection of physiological and biochemical parameters that are critical for the health of human beings. First, the main components and basic working principles of OECTs will be briefly introduced. In the following, the strategies and key technologies for the preparation of functional OECT-based biosensors will be outlined and discussed with regard to the applications of the detection of various targets, including metabolites, ions, neurotransmitters, electrophysiological parameters, and immunological molecules. Finally, the current main issues and future development trends of functional OECT-based biosensors will be proposed and discussed. The breakthrough in functional OECT-based biosensors is believed to enable such devices to achieve higher performance, and thus, this technology could provide new insight into the future field of medical and life sciences.

Deciphering supramolecular arrangements, micellization patterns, and antimicrobial potential of bacterial rhamnolipids under extreme treatments of temperature and electrolyte

The micellization properties of rhamnolipids (RLs) in extreme electrolyte concentrations and temperatures have gained considerable attention due to their broad industrial applications. In this study, the aggregation behavior, specifically the micellization pattern (critical micelle concentration (CMC)) of RLs produced from a newly isolated thermophilic strain of Pseudomonas aeruginosa from a harsh environment of an oil field, was investigated by a spectrophotometric method at various temperatures (293–393 K) and electrolyte concentrations (NaCl: 2–20%). The result indicated that the CMC values (0.267–0.140 mM⋅dm−3) were both electrolyte- and temperature-dependent exhibiting a U-shaped trend as temperature and NaCl concentration increased. Variations in NaCl concentration and temperature also affected the standard Gibbs free energy (ΔGomic), enthalpy (ΔHomic), and entropy (ΔSomic) of micellization. The molecule also showed stability at a broad range of temperatures, pH, and NaCl concentrations. Thin-layer chromatography (TLC) and Fourier-transform infrared (FTIR) analysis confirmed the similarity in composition between the crude extract and the commercial RL with Rf values of 0.72 for mono-rhamnolipids and 0.28 for di-rhamnolipids. FTIR analysis confirmed the chemical nature particularly key aliphatic functional groups present in the fatty acid tail of RLs and the -COC- bond in the structure of the rhamnose moiety. Additionally, LC-ESI-QTOF analysis confirmed corresponding ionic fragments of mono- and di-rhamnolipids congeners. Furthermore, the antimicrobial potential was determined against different human pathogens in the absence and presence of NaCl by measuring zones of inhibition. The result revealed enhanced inhibitory effects against Gram-positive pathogens (S. aureus, S. epidermidis, and L. monocytogene), with zones of inhibition of 26, 30, and 20 mm in the presence of NaCl. These findings underline the role of NaCl in the micellization of RL molecules and highlight their importance in environmental applications, pharmaceuticals, and various life science sectors.

Strategic policy options to improve quality and productivity of biomedical research.

Emerging societal expectations from biomedical research and intensifying international scientific competition are becoming existential matters. Based on a review of pertinent evidence, this article analyzes challenges and formulates public policy recommendations for improving productivity and impact of life sciences. Critical risks include widespread quality defects of research, particularly non-reproducible results, and narrow access to scientifically sound information giving advantage to health misinformation. In funding life sciences, the simultaneous shift to nondemocratic societies is an added challenge. Simply spending more on research will not be enough in the global competition. Considering the pacesetter role of the federal government, five national policy recommendations are put forward: (i) funding projects with comprehensive expectations of reproducibility; (ii) public-private partnerships for contemporaneous quality support in laboratories; (iii) making research institutions accountable for quality control; (iv) supporting new quality filtering standards for scientific journals and repositories, and (v) establishing a new network of centers for scientific health communications.

Burn your fat—a unique DIY board game about triacylglycerol breakdown

Understanding metabolic pathways is challenging for students due to their complex nature and intricate interdependencies. To address this problem, gamification has emerged as a promising approach to make scientific concepts accessible and engaging. We present a DIY educational activity focusing on lipid and glucose metabolism to clarify the basic principles of biochemical transformations and provide practical educational resources for lecturers. The activity is designed to be accessible and requires minimal resources, using physical media such as printed boards and dice. It consists of two parts: an introductory session where students build a triacylglycerol molecule and a board game where teams compete to metabolically break down triacylglycerols and obtain ATP. The process in governed by rules based on metabolic transformations, with players taking on roles as walkers, counters, transformers, and strategists to encourage cooperation. The game aligns with the theories of cognitivism and constructivism and emphasizes knowledge acquisition, the formation of mental structures, and active learning through experience. It promotes a socio-cultural perspective and the development of interpersonal skills by encouraging teamwork, cooperation, and problem-solving. The thematic focus on obesity provides real-world context, and the DIY concept, 3D printing capability, and potential for creating extensions encourage student engagement and customization. The activity effectively teaches metabolic networks to high school students, is helpful for graduate students in visualizing experimental problems, increased engagement in the classroom and interest in the life sciences. A simplified version has also been used for the general public at science fairs, highlighting its versatility and accessibility.

Into the Buzzing World: Integrating Artwork with Fieldwork to Learn About Bees

This article describes a pedagogical approach that integrated artistic expression with experiential fieldwork to enhance pupils’ learning of bees. Initially, pupils were tasked with creating an artwork of a bee or bees based on their preconceived notions, allowing teachers to identify their prior ideas of bees. Afterward, an immersive field activity was conducted to provide pupils with an opportunity to close-up observe live honey and native bees in canola fields and their pollination processes. Through teacher-guided explorations, pupils systematically inspected the morphology of various bee species, relating their external structures to associated functions. The fieldwork allowed for detailed observations of bees’ features, such as the proboscis, antennae, and legs, and their roles in nectar collection and pollen distribution. In addition, exposure to honey and native bees informed pupils of the differences in their stinging, nesting and sociality. Pupils’ summative reflections post-fieldwork suggest that the immersive fieldwork enabled multi-sensory modes of learning barely available in a traditional classroom setting. By juxtaposing the new insights from field observations with the prior ideas of bees embodied in their initial artworks, pupils achieved gains in both their conceptual understandings and perceptual knowledge about bees. Pupils acknowledged deeper understandings of bee anatomy, pollination processes and the diversity within bee species. This approach to learning about bees epitomizes the value of empirical investigation for advancing pupils’ scientific practices and understanding life science core ideas of how animals' external structures fulfill various functions and of how plants and pollinators are interdependent, as highlighted in <i>the Next Generation Science Standards</i>. The integration of artwork creation and experiential fieldwork exemplifies an effective pedagogical approach for enhancing pupils' engagement and learning of biological ideas.

Artificial Intelligence-Assisted Automatic Raman-Activated Cell Sorting (AI-RACS) System for Mining Specific Functional Microorganisms in the Microbiome.

The microbiome represents the natural presence of microorganisms, and exploring, understanding, and leveraging its functions will bring about significant breakthroughs in life sciences and applications. Raman-activated cell sorting (RACS) enables the correlation of phenotype and genotype at the single-cell level, offering a solution to the bottleneck in microbial community functional analysis caused by challenges in cultivating diverse microorganisms. However, current labor-intensive manual procedures fall short in catering to the demands of single-cell functional analysis in microbial communities. To address this issue, we developed an artificial intelligence-assisted Raman-activated cell sorting system (AI-RACS) that integrates precise single-cell positioning, automated data collection, optical tweezers capture, and single-cell printing to elevate microbial single-cell RACS from manual to automated, validating the efficacy of the system by isolating aluminum-tolerant microbes from acidic soil microbiota. Leveraging the AI-RACS framework, we sorted 13 strains from red soil samples under near-in situ conditions, with all demonstrating strong aluminum tolerance. AI-RACS efficiently segregates microbial cells from intricate environmental samples, investigating their functional attributes and presenting a novel tool for microbial research and applications.

Value-Based Healthcare in Practice: IDEATE, a Collaboration to Design and Test an Outcomes-Based Agreement for a Medicine in Wales.

To develop a sustainable, scalable methodology for the design of outcome-based agreements (OBAs) that works on the ground and dynamically overcomes historical challenges. Project IDEATE co-created solutions to known (and emergent) challenges via iterative workshops and real-world data analysis to develop and refine a hypothetical model for an OBA in a trusted research environment. A cross-disciplinary collaboration between National Health Service (NHS) Wales, industry and academia was developed. Data were collected from Welsh national datasets and used to construct a novel linked dataset. OBA scenarios, with different contract parameters, were analysed to assess impact on the proportion of contract payment due and the volatility of payments. An approved, in market, locally advanced and metastatic breast cancer treatment was selected as the test case. The total number of patients in the treatment cohort (2017-2020) was n = 99, and 286 in the control cohort (2014-2016). The final outcome variables selected were: (1) 1-year survival,( 2) intolerance to treatment (deferral), and (3) the total days disrupted by care. The primary scenario included all three outcomes measured at the population level and used a linear payment model. Volatility analyses demonstrated contract parameters can dramatically alter the contract output with greatest risk from a single, binary outcome contract design. The design of an OBA is a complex process that requires a multi-disciplinary approach. By assessing solutions to data, outcomes and contracting challenges, IDEATE provides a strong foundation for future success of OBAs in the UK. Outcome-based agreements (OBAs) are a way to pay for medicines if they help patienthealth in a specific way over time. These agreements can make it faster for people to get new medicines, but they also have challenges, like needing a lot of time and effort to manage them. A team from the NHS Wales, life sciences, and Swansea University created Project IDEATE to find a better way to design OBAs and solve some of these problems. Welsh datasets were used to create a new breast cancer dataset to test different OBAs and see how payments would change. A breast cancer treatment was used for the project. The project had 99 patients who got the medicine (2017-2020) and 286 patients who had breast cancer but did not get the medicine (2014-2016). Three health outcomes were measured: (1) living for one year after treatment, (2) patients needing to stop the medicine, and (3) days spent in care. The main OBA option we tested used all three health outcomes; the more the outcomes improved, the more the payments could go up until they hit the highest amount agreed. The analysis showed that the way an OBA is designed can make a big difference in how stable or risky it is, especially if one ofthe health outcomes has only two options. Project IDEATE showed that making an OBA can be hard, but when people from different fields work together, they can overcome many challenges and succeed.

BIOM-05. FUSION TRANSCRIPTOME LANDSCAPE IN GLIOBLASTOMA

Abstract BACKGROUND For most patients with glioblastoma (GBM), no truly effective salvage therapies exist. However, there are compelling examples in oncology where gene fusions lead to functioning oncoproteins that are highly effective targets (ie BCR-ABL1 in CML). Using a large genomic database, we sought to determine the prevalence of fusion transcripts in GBMs. METHODS A total of 4392 GBMs were tested at Caris Life Sciences (Phoenix, AZ). Mutations were detected by NextGen sequencing of DNA (592-gene panel or whole exome sequencing); gene fusions were detected by Whole Transcriptome Sequencing. All tumors were wild type for IDH1/2. Significance was tested by Fisher-Exact and ChiSquare and adjusted by Benjamini-Hochberg (q <0.05). RESULTS Overall, pathogenic fusions were found in 428 (9.7%). The most prevalent were FGFR3 [N=159 (37%), highest FGFR3:TACC3, N=134], MET [N=92 (21%), highest PTPRZ1:Met, N=31; ST7:MET N=25; CAPZA2: MET, N=23), EGFR [N=87(20%), highest EGFR:SETP14, N=21 and SEC61G: EGFR, N=19], NTRK2 (N=27), PDGFRA (N=23), ROS1 (N=14) and BRAF (N=10). Other actionable fusions including ALK (N=3), NTRK3 (N=3), RAF1(N=3), RET (N=3), NTRK1(N=2) were seen. Tumors with fusions were more likely to have pathogenic gene amplifications including: MET (7.5% vs. 0.7%), FGFR3 (5% vs. 0.2%), CDK4 (17% vs. 11%) and MDM2 (12% and 7.5%) but less frequent EGFR mutation (6.1% vs. 18%), amplification (6.1% vs. 18%) and EGFRvIII mutation (11.9% vs. 22.5%), RB1 (2.9% vs. 11.5%) and TP53mutation (22% vs. 30%) (all q<0.05). EGFR fusions are enriched with EGFR amplification (92%) and EGFRvIII mutation (51%); cMET fusion with MET amplification (40%). CONCLUSIONS Comprehensive molecular profiling reveals that approximately 10% of IDH WT GBMs carry oncogenic fusions that may be therapeutic targets. Broad spectrum of observed fusions underscores the need for novel clinical trial designs to allow efficient enrollment for prospective testing of potential targeted agents.

Database resources of the National Center for Biotechnology Information in 2025.

The National Center for Biotechnology Information (NCBI) provides online information resources for biology, including the GenBank® nucleic acid sequence repository and the PubMed® repository of citations and abstracts published in life science journals. NCBI provides search and retrieval operations for most of these data from 31 distinct repositories and knowledgebases. The E-utilities serve as the programming interface for most of these. Resources receiving significant updates in the past year include PubMed, PubMed Central, Bookshelf, the NIH Comparative Genomics Resource, BLAST, SequenceReadArchive, Taxonomy, iCn3D, Conserved Domain Database, Pathogen Detection, antimicrobial resistance resourcesand PubChem. These resources can be accessed through the NCBI home page at https://www.ncbi.nlm.nih.gov.

Experiences of treating problematic over the counter and prescription only medication use in substance misuse services

Abstract Introduction There are growing concerns about the misuse of over the counter (OTC) and prescription only medication (POM), due to the associated negative socioeconomic and health implications1. To improve care delivery, stigma needs to be addressed and more needs to be known about the perspectives of staff who are working in substance misuse services (SMS)2. Aims To use confidential semi-structured interviews, to explore the experiences of community English SMS staff who have supported adults that have misused OTC/POM. Methods This research was conducted across five community adult English SMS operated by a national treatment provider. Individuals were eligible for participating in semi-structured interviews if they had current experience of working in a community SMS role which involved supporting adults who had misused OTC/POM, were at least 18 years of age and provided informed consent. Potential participants self-identified as being eligible and interested in being included, after receiving the participant information sheet. The interviews explored their experience of working in SMS and supporting people who have misused OTC/POM, and potential changes to current SMS provision. Thematic analysis of the audio-recording transcripts was undertaken using NVivo®. This research was funded by a Research Award from Pharmacy Research UK and the College of Mental Health Pharmacy [PRUK_CMHP-2019-2-RG]. This research was approved by Humankind/EDP and Aston University’s Life and Health Sciences Ethics Committees (ID#1655). Results Organising interviews was challenging due to pandemic-related work pressures, between October 2020 and January 2021. Thematic analyses of twenty SMS staff transcripts was undertaken and data saturation was thought to have been achieved. Three overarching themes were identified: characteristics of OTC/POM misuse; different groups of people; and negative experiences (with six sub-themes: lack of awareness of risk of harm; unmanaged health conditions; withdrawal symptoms; side-effects; problematic behaviours; and treatment pathways). Oral dependent use of opioids (especially codeine products), benzodiazepines and gabapentinoids predominated. Polypharmacy and the use of other substances sometimes occurred. OTC/POM misuse was associated with a wide range of adverse socioeconomic, physical, and psychological effects. Unmanaged withdrawal symptoms caused concern, contributed to perpetuating misuse, and sudden cessation in supplies created risks. Discussion and Conclusion This study had a unique focus on the experiences of third sector English SMS staff. OTC/POM misuse (and withdrawal symptoms) should be routinely enquired about during healthcare reviews, and tailored harm reduction, pharmacological and psychosocial interventions offered. Increased awareness, vigilance for associated safeguarding issues, and more training is required. SMS should sensitively consider how to incorporate the views of staff with lived experience to further optimise service delivery co-production. As staff were all employed by the same SMS provider, future research, conducted in SMS operated by other providers, and covering a wider geography, may enable more information to be shared, more participants to be recruited, and the results more generalisable. Repeat interviews and member-checking may improve assurance in the findings. The possible need for different SMS for those who only misuse OTC/POM without exacerbating stigma requires further exploration. Future research should identify if demographic characteristics and treatment needs differ by OTC/POM type.

Biothermodynamics of Hemoglobin and Red Blood Cells: Analysis of Structure and Evolution of Hemoglobin and Red Blood Cells, Based on Molecular and Empirical Formulas, Biosynthesis Reactions, and Thermodynamic Properties of Formation and Biosynthesis.

Hemoglobin and red blood cells (erythrocytes) have been studied extensively from the perspective of life and biomedical sciences. However, no analysis of hemoglobin and red blood cells from the perspective of chemical thermodynamics has been reported in the literature. Such an analysis would provide an insight into their structure and turnover from the aspect of biothermodynamics and bioenergetics. In this paper, a biothermodynamic analysis was made of hemoglobin and red blood cells. Molecular formulas, empirical formulas, biosynthesis reactions, and thermodynamic properties of formation and biosynthesis were determined for the alpha chain, beta chain, heme B, hemoglobin and red blood cells. Empirical formulas and thermodynamic properties of hemoglobin were compared to those of other biological macromolecules, which include proteins and nucleic acids. Moreover, the energetic requirements of biosynthesis of hemoglobin and red blood cells were analyzed. Based on this, a discussion was made of the specific structure of red blood cells (i.e. no nuclei nor organelles) and its role as an evolutionary adaptation for more energetically efficient biosynthesis needed for the turnover of red blood cells.

MPharm students’ views and experiences of the teaching of pharmaceutical calculations and their importance in pharmacy practice

Abstract Introduction Accurately undertaking pharmaceutical calculations is an important professional skill as part of a pharmacist’s role ensuring patient safety1. Effective teaching in this area supports safe, high-quality care from future pharmacists. In the United Kingdom, the need to accurately perform calculations is stipulated in the General Pharmaceutical Council (GPhC) standards for initial education and training of pharmacists2. During a review of teaching in this area, the views and experiences of pharmacy students were sought, in order to inform course design. Aim This project aimed to explore undergraduate MPharm students’ views regarding the importance of pharmaceutical calculations to their future careers, experiences of current calculations teaching, and opinions on improving course design. Methods A questionnaire was developed and distributed to Years 1-4 pharmacy students (n=524) at the School of Pharmacy, Queen’s University Belfast (QUB), in December 2023. The questionnaire consisted of 45 questions, collecting demographic information, as well as students’ views and experiences of pharmaceutical calculations, various teaching methods, and student confidence with calculations. Data was transcribed into Microsoft Excel, followed by analysis using descriptive statistics in IBM SPSS. Ethical approval was received from the QUB Faculty of Medicine, Health & Life Sciences Research Ethics Committee (MHLS23_159). Results A total of 271 students responded to the questionnaire, providing a response rate of 52%. Students felt that being able to perform calculations is important to their future career, with 97% of respondents agreeing or strongly agreeing with this statement. Hospital pharmacy was considered the sector where calculations were most important (96% agree/ strongly agree). Overall, 75% of respondents agreed/ strongly agreed that they were confident in undertaking pharmaceutical calculations. Students felt most competent using provided formula, and with dose and dosage regimen calculations. Conversely, students felt least competent performing displacement value and dilutions questions. With respect to teaching design, students preferred blended-learning to self-directed study or traditional lectures. Eighty-seven percent of respondents reported blended-learning as beneficial/ very beneficial, compared to 57% and 46% for the other approaches. Students reported formative quizzes as the most beneficial activity for learning (92% beneficial/ very beneficial). This was followed by provision of worked examples, with a preference for handwritten solutions. Recommended textbooks and online discussion boards were considered least beneficial. Having dedicated introductory calculations teaching in Year 1, with repeated opportunities for practice and support in subsequent years had the most support for improving teaching, with 87% stating this would be beneficial/ very beneficial. There was also support for the NUMBAS3 e-assessment system for formative examples, along with video or pencasted worked examples, with 80% and 70% of respondents respectively classing these as beneficial/ very beneficial. Discussion / Conclusion QUB MPharm students appreciate the importance of pharmaceutical calculations to their future careers. They prefer a blended-learning approach to teaching, incorporating formative quizzes and worked examples. Dedicated Year 1 teaching, the use of NUMBAS, and pencasting were identified as methods to improve teaching. These findings will aid future course design, with the aim of improving patient safety relating to pharmaceutical calculations.

Exploring the role of primary care pharmacists in NHS Lanarkshire following the implementation of the pharmacotherapy hub: a service evaluation

Abstract Introduction The General Medical Service (GMS) contract was produced in Scotland in an attempt to tackle General Practitioner (GP) workforce issues. The pharmacotherapy work stream is part of this and provides a framework for pharmacy teams, dividing services into level 1 (core), level 2 (advanced) and level 3 (specialist).1 A statement by the Royal Pharmaceutical Society (RPS) and the British Medical Association (BMA) discussed the introduction of pharmacotherapy hubs to complete level 1 work for multiple practices, centralising the medicines reconciliation process. Theoretically, this would release pharmacists to carry out clinical and more advanced/specialist services.2 There are currently 8 pharmacotherapy hubs within NHS Lanarkshire (NHSL), incorporating level 2 and 3 services into job plans for General Practice Clinical Pharmacists (GPCPs). No work has been undertaken to explore changes in practice since the implementation of these hubs. The qualitative study explored drivers and motivators for pharmacists in practice, shaping future pharmacotherapy services in NHSL and Scotland. Aim To explore the views and attitudes of primary care pharmacists towards their practice role in NHSL following the implementation of the pharmacotherapy hub. Methods Ethical approval was granted from the School of Pharmacy and Life Sciences Ethics Committee (Robert Gordon University). Pharmacists working within the 8 hub localities were invited to participate via email correspondence if they had over 6 months experience in practice pre-hub implementation and then 6 months post-hub implementation. Participants attended a one-to-one interview to share their views towards their practice role since the hub was implemented. Interviews were recorded, transcribed verbatim and thematically analysed. Results Twelve pharmacists across six localities participated. The five key themes identified were job role, managing time, professional development, professional belonging and patient care. The implementation of the hub was an enabler for pharmacists to partake in advanced pharmacotherapy tasks. Pharmacists negatively perceived level 1 core pharmacotherapy tasks in comparison to level 3 clinical patient-facing time. Participants linked the hubs impact on practice with role development, an increase in autonomy, confidence and job satisfaction. Discussion/Conclusion The hub has enabled in-practice pharmacists to move to a more sustainable balance between core and advanced/specialist tasks. Collaboration is required between pharmacy teams and practices to understand the pharmacist role in pharmacotherapy. Further recommendations include continuing the roll-out of pharmacotherapy hubs, standardisation of pharmacist clinical time balanced with core tasks and implementation of protected learning time for pharmacy teams. The study was open to response bias but this was addressed by using semi-structure interview guides in the one-to-one interviews. Researcher bias was minimised by two researchers analysing the data and practicing reflexivity. The study was open to recall bias due to participant’s retrospective thinking. In NHSL prioritising the hub roll-out and collaboration with stakeholders are essential. This will ensure the balance between efficient pharmacotherapy services and maintaining attractive roles, contributing to a sustainable workforce.

Change point detection in temporal networks based on graph snapshot similarity measures

This paper addresses the challenge of change point detection in temporal networks, a critical task across various domains, including life sciences and socioeconomic activities. Continuous analysis and problem-solving within dynamic networks are essential in these fields. While much attention has been given to binary cases, this study extends the scope to include change point detection in weighted networks, an important dimension of edge analysis in dynamic networks. We introduce a novel distance metric called the Interval Sum Absolute Difference Distance (ISADD) to measure the distance between two graph snapshots. Additionally, we apply a Gaussian radial basis function to transform this distance into a similarity score between graph snapshots. This similarity score function effectively identifies individual change points. Furthermore, we employ a bisection detection algorithm to extend the method to detect multiple change points. Experimental results on both simulated and real-world data demonstrate the efficacy of the proposed framework.

Necessary reforms in the Greek academic system

Motivated by our EU funded ERA chair projects, that require proposing reforms to yield the Greek academic system more competitive with a particular emphasis on fostering brain gain and preventing brain drain, we propose policy changes on a wide range of topics, based on our joint academic management experience inside and outside of Greece in conjunction with our diverse backgrounds in the humanities, computer science, and the life sciences. The proposed reforms can contribute to increase the competitiveness of Greece in research and development (R & D). We classify proposals by their associated costs and include a plethora of zero cost proposals that do have the potential to increase efficiency and net research time as well as to improve current working conditions in Greek academia. However, to yield Greece more competitive at the international level, reverse brain drain, and foster brain gain, substantial investments and increases of R&D expenditure are required which depend on political willingness and require a long term strategic development plan for Greece beyond being a tourist destination in the European periphery.

Recent Advances in NIR-Switchable Multi-Redox Systems Based on Organic Molecules.

In recent years, near-infrared (NIR) dyes, exhibiting absorption in the NIR region (750-2500 nm), have been applied to various optical applications such as security marking, photovoltaic cells and chemotherapy of deep tissues in vivo. Electrochromic systems capable of switching NIR absorption are attractive from the viewpoint of applications for material and life science, and thus several examples have been reported to date. The development of organic-based materials is needed to reduce the environmental impact and improve biocompatibility, however, the switching of NIR absorption based on redox interconversion is still a challenging issue regarding reversibility and durability during interconversion. To overcome this potential instability, several studies on organic electrochromic systems that allow ON/OFF switching of NIR absorption have been developed in recent years. In this review, we focus on redox-active well-defined small molecules that enable ON/OFF switching of NIR absorption, and present recent studies on their intrinsic electrochemical and spectroscopic properties and/or structural characterization of their charged states. We also address more sophisticated electrochromic systems that can modulate their properties in response to external stimuli such as light, heat, and electric potential.

Case report: High grade serous fallopian tube carcinoma with rare NRG1 gene fusion presenting as widespread peritoneal carcinomatosis

The discovery of gene fusions involving Neuregulin-1 (NRG1) within solid tumors has important therapeutic implications, as they are being actively explored as targets for emerging ERBB/ERBB2/ERBB3-directed anti-cancer agents. NRG1 fusions are very uncommon across all tumor types and are infrequently documented in the medical literature. We report a female patient presenting with widespread peritoneal carcinomatosis diagnosed as high grade serous fallopian tube carcinoma, which harbored a previously undescribed MYH10::NRG1 fusion. Moreover, we queried the whole transcriptome sequencing results of neoplasms analyzed by a commercial laboratory (Caris Life Sciences) to determine the overall incidence of NRG1 fusions in carcinomas of the ovary, fallopian tube, and peritoneum (0.18%). Twenty-five additional tumors were found to demonstrate NRG1 fusions, including 20 new genes partners that had not been previously identified in gynecologic carcinomas. Overall, NRG1 fusion events are rare in ovarian, fallopian tube, and primary peritoneal carcinomas, but they may carry diagnostic significance in the context of borderline/low grade serous tumors, which demonstrated exclusively CLU::NRG1 fusions, and could have important predictive implications for response to ERBB/ERBB2/ERBB3-directed therapies.