Life Sciences Research Articles

The Tropic-Concepts of Life: Jacques Derrida’s Contribution to the Question of Metaphor in the Life Sciences

In the field of life science there is a growing awareness of the metaphorical tenor of many of its key concepts and the problems that arise from them, as in the now-controversial case of the genetic ‘program’. In the seminar Life Death (2019) Derrida addresses this question from Georges Canguilhem’s difficulties in establishing a clear distinction between metaphor and concept, precisely with regard to the notion of ‘program’, concluding that this difficulty ‘demand[s] a recasting of this division and of the law of this division’ between metaphor and concept. Beginning with these pages from Life Death, the present essay aims to reconstruct the deconstruction of this opposition and its epistemological consequences by going back to the Derridean essay ‘White Mythology’ (1971), to which Derrida refers in the seminar and in which the question of metaphor in the life sciences had been addressed in a critical confrontation with Bachelard and Canguilhem.

Knee Joint Dynamics: An Experiment to Introduce the Concept of Torque to Biomedical and Life Sciences Students

Knee Joint Dynamics: An Experiment to Introduce the Concept of Torque to Biomedical and Life Sciences Students

Next‐Generation Time‐Resolved Electron Paramagnetic Resonance for Direct Visualizing Mechanisms in Radical Chemistry

AbstractRadical chemistry is an important research field that plays a vital role in life science, material science, and energy conversions. However, directly visualizing the transient open‐shell intermediates unambiguously in radical chemistry is a significant and long‐standing challenge. Recently, we have developed an innovative ultrawide single‐sideband phase‐sensitive detection (U‐PSD) time‐resolved electron paramagnetic resonance (TREPR) technique, which enables the characterization of diverse transient radical intermediates and mechanistic studies close to reaction conditions. In this brief review, we will offer an outline of the principles underlying our U‐PSD TREPR technique, and present examples highlighting the technique's ability to accurately characterize and track free radical intermediates in photocatalytic reactions.

5.E. Round table: Crafting Policies for Health Promotion: evidence from local settings

Abstract As communities vary in their needs, challenges, and resources, tailoring health promotion policies to specific contexts becomes imperative for effectiveness. Drawing upon the expertise of seasoned practitioners, policymakers, and researchers, this workshop offers a multifaceted perspective on navigating the complexities inherent in designing, implementing, and evaluating locally tailored health promotion policies, with a specific focus on environmental and social factors. The workshop will make it possible to compare different experiences from various European countries, with high-level speakers who have different backgrounds and roles in their countries and regions, thus providing an interesting and interactive way of comparing and examining real-world examples and best practices. The examples provided delve into: participatory techniques to design healthier environments, to identifying, collecting and elaborating evidence upon which to build policies and actions that are effective in addressing specific health challenges. Through the examples presented, we will seek to make the case for multi-actor and multisectoral collaboration. In addition, we will try to understand How do subnational authorities and partners identify priorities for action. A common question asked to the speakers will therefore: how can a variety of actors (local and regional authorities, university and research centres...) come together to support cross-sectoral action? How can policymakers be supported towards creating environments that prioritize the well-being of all, ensuring a healthier and more prosperous future for everyone? The end goal will be to enable attendees to be equipped with actionable strategies and tools to foster meaningful change within their own communities, by understanding the intricate interplay between community dynamics, stakeholder engagement, evidence-based practices, and policy implementation strategies. The workshop is being organized by the WHO Regions for Health Network (RHN). Key messages • Tailoring health promotion policies to specific contexts is imperative for effectiveness, considering the diverse needs, challenges, and resources of different communities. • Subnational authorities and partners must identify priorities for action and collaborate across sectors to address specific health challenges effectively. Speakers/Panelists Klara Dokova Medical University of Varna, Varna, Bulgaria Camilla Ihlebaek Norwegian University of Life Sciences, Aas, Norway Jo Peden Public Health Wales, Cardiff, UK Odile Mekel NRW Centre for Health, Bochum, Germany Liesbeth van Holten Province of Utrecht, Utrecht, Netherlands

On the Use of Image Analysis for Hematocrit Evaluation in Dried Blood Spots

Dried blood spots (DBSs) are formed by collecting a small sample of blood on specialized filter paper and allowing it to dry naturally. Various domains of life sciences and drug research extensively use DBSs as a sampling technique. The “Hematocrit (Ht) effect” affects assay bias, and several strategies have been put forth to deal with it, including the correction of quantified concentrations using an appropriate correction factor. The approach was previously applied, following the utilization of an image processing algorithm developed in Matlab® to derive a reliable equation correlating DBS areas to Ht% values. The present work looks more closely at the application of image analysis to the evaluation of Ht in DBS samples. Utilizing image analysis software, DBS samples with known Ht values were processed. Preparation of cards has followed a previously developed protocol for the appropriate formation of uniform area DBSs, irrespective of Ht. The resulting areas showed close resemblance to the respective theoretical areas calculated by applying the correlation equation. Following that, the equation was utilized to determine the Ht values for each sample, and a comprehensive comparison of measured versus calculated Ht was carried out using various statistical approaches for method comparison. The results demonstrated a strong correlation, suggesting the method’s viability in estimating Ht for unknown DBS samples.

All-cause, cancer and cardiovascular mortality among South Asians living in the UK: Follow-up of the London Life Sciences Population (LOLIPOP) cohort

Abstract Background Although South Asians are the largest ethnic minority group in the UK, and the largest ethnic group worldwide, few studies have investigated patterns of mortality in South Asians. Methods We investigated 16,391 South Asian and 6,970 European men and women living in the UK, and participating in the London Life Sciences Prospective Population study. Participants were recruited in 2002-2008, and survival determined to 2019. We examined relationships of clinical, anthropometric, and lifestyle risk factors to mortality outcomes. Findings: Compared to Europeans, South Asians had higher prevalence of diabetes, cardiovascular disease, and hypertension, higher waist-hip ratio, fasting glucose, insulin and triglycerides, but lower smoking rates and HDL cholesterol (all P<0.001). Age and sex adjusted all-cause mortality was lower (Hazard Ratio (HR): 0.75 [0.68-0.81], P<0.001), all-cancer mortality was lower (HR 0.50 [0.43-0.58], P<0.001), and coronary heart disease (CHD) mortality was higher (HR 1.29 [1.05-1.60], P<0.01) in South Asians compared to Europeans. Mortality rates were ~50% lower for 9 of the 10 most common cancers in South Asians. After adjusting for risk factors, ethnic differences were attenuated only for CHD mortality. Interpretation: All-cause and cancer mortality are lower, but CHD mortality is higher, in South Asians compared to Europeans. Measured risk factors do not explain lower all-cause and all-cancer mortality, but do account for higher CHD mortality. Our findings provide evidence for population-specific exposures protective against cancers in South Asians, as well as the motivation to further improve cardiovascular risk reduction in this major ethnic group.

Procrastination and risky behaviors: preliminary results among Italian university students

Abstract Background The study aims to investigate if the tendency to procrastinate has a relationship with engaging in risky behaviors among university students. Methods A cross-sectional study was conducted at the ‘Magna Græcia’ University of Catanzaro, in the Southern part of Italy, using a stratified random sample technique. Undergraduate students (ages 18 to 30) were provided with an anonymous online survey via the institutional email. The survey comprised five sections to assess procrastination levels, sleep quality, breakfast habits, alcohol and tobacco use, and social and demographic characteristics. Results Of the 404 participants, more than half were enrolled in medicine or life science majors. The procrastination mean score was 14.8(IQR 10-20). Poor sleep quality was found in 57.2% of students and women were the most affected part of the sample. The logistic regression model revealed that higher procrastination levels increased the likelihood of engaging in unhealthy behaviors such as breakfast skipping, alcohol abuse, and poor sleep quality. Furthermore, the study found that people who skipped breakfast were more likely to engage in smoking and alcohol abuse, suggesting the interconnectedness nature of unhealthy behaviors. Conclusions The study’s findings shed light on the correlation between procrastination and unhealthy habits. Moreover, the results can offer recommendations for how universities might successfully support students by implementing integrated, evidence-based programs to assist students who struggle with procrastination. Indeed, universities can serve as centers for promoting wellness by increasing awareness of the need to change deeply rooted behavioral patterns. Hence, breaking the vicious cycle of procrastination can ultimately lead to improved academic performance and overall well-being for students. This will benefit both individuals’ and the general public’s health. Key messages • The novel correlation between procrastination and risky behaviors among university students was shown. • Targeted interventions must include procrastination tendency to improve academic results and general well-being.

Benefits of cross-border access to human genomes at scale for research and healthcare

Abstract Genomics data will soon be routinely generated and integrated into national healthcare systems. To maximise the potential of genomic medicine, data should be accessible for research where possible. This is the remit of ELIXIR, an intergovernmental organisation that brings together life science resources from across Europe. Innovative solutions are needed to ensure the validated research findings for disease or preventative medicine are then integrated into healthcare. To tackle this, the 1+MG initiative, a joint initiative of 25 EU countries, the UK, and Norway, aims to enable secure access to genomics and the corresponding clinical data across Europe for better research, personalised healthcare and health policy making. In the design and scale-up phase (B1MG project), recommendations and guidelines to advance towards the deployment of personalised medicine at a European scale have been produced, adopted by 1+MG and developed into a 1+MG framework. This includes guidance on data governance, standards, quality and infrastructure, recommendations on how to approach citizen engagement and a tool for countries to self-assess implementation into healthcare. The European Genomic Data Infrastructure (GDI) project supports the scale-up and sustainability phase of the 1+MG initiative, to deploy infrastructure across 24 countries to support the overall ambition. Recommendations are being used to promote governance and technical interoperability across European initiatives including the European Health Data Space (EHDS), and European Cancer Image Initiative (EUCAIM). The 1+MG will be established as a European Data Infrastructure Consortia in 2025 and will act as an Authorised Participant in the EHDS providing access to a permanent high-quality federated data collection of genomic and health data that will accelerate research, innovation and policymaking facilitating the deployment of genomic medicine across Europe.

Improving ncRNA family prediction using Multi-Modal contrastive learning of sequence and structure.

Recent advancements in high-throughput sequencing technology have significantly increased the focus on non-coding RNA (ncRNA) research within the life sciences. Despite this, the functions of many ncRNAs remain poorly understood. Research suggests that ncRNAs within the same family typically share similar functions, underlining the importance of understanding their roles. There are two primary methods for predicting ncRNA families: biological and computational. Traditional biological methods are not suitable for large-scale data prediction due to the significant human and resource requirements. Concurrently, most existing computational methods either rely solely on ncRNA sequence data or are exclusively based on the secondary structure of ncRNA molecules. These methods fail to fully utilize the rich multimodal information available from ncRNAs, thereby preventing them from learning more comprehensive and in-depth feature representations. To tackle these problems, we proposed MM-ncRNAFP, a multi-modal contrastive learning framework for ncRNA family prediction. We first used a pre-trained language model to encode the primary sequences of a large mammalian ncRNA dataset. Then, we adopted a contrastive learning framework with an attention mechanism to fuse the secondary structure information obtained by graph neural networks. The MM-ncRNAFP method can effectively fuse multi-modal information. Experimental comparisons with several competitive baselines demonstrated that MM-ncRNAFP can achieve more comprehensive representations of ncRNA features by integrating both sequence and structural information. This integration significantly enhances the performance of ncRNA family prediction. Ablation experiments and qualitative analyses were performed to verify the effectiveness of each component in our model. Moreover, since our model is pre-trained on a large amount of ncRNA data, it has the potential to bring significant improvements to other ncRNA-related tasks. MM-ncRNAFP and the datasets are available at https://github.com/xuruiting2/MM-ncRNAFP.

Examining the potential of in- and extra-curricular challenge-based learning in higher education: A delphi study

ABSTRACT Educating students in higher education who are capable of addressing societal challenges requires innovative learning journeys. In the current study, we explore challenge-based learning (CBL) as one way to realise such education. A Delphi study has been conducted at a Dutch University for Life Sciences to identify key characteristics, learning activities, learning outcomes and support of in- and extra-curricular CBL from a stakeholder perspective. Data were collected in two rounds (round 1; N = 47; round 2; N = 29). In-curricular CBL allows students to experience guided teamwork in relation to societal issues as part of their formal curriculum. Extra-curricular CBL provides opportunities for students to have their own, customised learning journey, allowing for strong societal involvement and a variety of learning gains. In- and extra-curricular CBLs have similarities but serve different learning outcomes. Both could be part of developmental lines preparing students to be change agents for sustainability in their future life.

A new species of the genus Thelcticopis Karsch, 1884 (Araneae: Sparassidae: Sparianthinae) from Guangdong, China

A new species of the genus Thelcticopis Karsch, 1884 is described from Guangdong Province, China: T. recta sp. nov. (♂, ♀). Diagnosis, morphological description, photos and distribution map of the new species are provided. Specimens are deposited in the Centre for Behavioral Ecology and Evolution, College of Life Sciences, Hubei University in Wuhan.

The Influence of Crossbreeding on the Composition of Protein and Fat Fractions in Milk: A Comparison Between Purebred Polish Holstein Friesian and Polish Holstein Friesian × Swedish Red Cows

Background/Objectives: In this study, the differences in protein and fat bioactive components between the milk from purebred Polish Holstein Friesian (PHF) cows and PHF cows crossbred with Swedish Red (SRB) were investigated. The objective was to assess the impact of genetic variation on the nutritional quality of their milk. Methods: This study was conducted at the Warsaw University of Life Sciences’ (WULS) experimental dairy farm in Warsaw, Poland, and involved 60 primiparous cows divided into two groups: 30 PHF×SRB crossbred cows and 30 purebred PHF cows. All cows were housed in a free-stall system with an average lactation yield exceeding 10,000 kg/lactation. The milk composition analyses included total protein, casein, whey protein, fatty acid profiles, and vitamin content. Results: Milk from the PHF×SRB hybrids showed a significantly greater total protein content (3.53%) compared to that from the purebred PHF cows (3.28%). The casein content was higher in the hybrids’ milk (2.90%) than the purebreds’ milk (2.78%), while the whey protein levels were lower in the purebred milk (0.50%) than in the hybrid milk (0.63%). The hybrids exhibited higher concentrations of certain saturated fatty acids in their milk, while the purebreds’ milk contained greater amounts of beneficial unsaturated fatty acids and fat-soluble vitamins—E, D, and K. Conclusions: These results indicate that genetic selection through crossbreeding can enhance the nutritional quality of milk. The differences observed in protein, fatty-acid, and vitamin content underscore the role of the genotype in milk composition, suggesting that breeding strategies can optimize dairy products’ health benefits.

Advancing quinoxaline chemistry: Sustainable and green synthesis using L-arabinose as a catalyst

In the present study, an innovative and eco-friendly methodology for synthesizing quinoxaline derivatives has been introduced, leveraging L-arabinose as a novel catalyst. This approach not only underscores the feasibility of green chemistry in synthesizing high-purity quinoxaline derivatives but also highlights the enhanced sustainability of the process. Detailed mechanistic investigations revealed the synergistic interactions between L-arabinose and the reactants, providing a deeper understanding of the catalytic process. The synthesized compounds, due to their unique structural and electronic properties, may find promising applications in life and material sciences such as optoelectronics. Furthermore, our environmental impact assessment demonstrated significant reduction in generated waste, solvent use, and overall carbon footprint, aligning with the global agenda for sustainable development. This research marks a significant step forward in quinoxaline chemistry, promoting the adoption of green synthesis pathways while reinforcing the principles of sustainable and environment-friendly chemical practices.

Investigation of 3rd Grade Life Science Textbook Texts and Activities According to Lipman's Critical Thinking Theory

Aim: Individuals need to have a philosophical critical thinking skill in order to have a reasonable approach to the situations and events they will encounter throughout their lives and to benefit themselves and the society Philosophical critical thinking is a process that enhances individuals' capacity to interrogate, analyse, and assess. In this regard, an inquisitive critical approach acquired at an early age makes important contributions to personality development. The current study aims to examine the text and activity contents in the 3rd grade Life Science textbook according to Matthew Lipman's critical thinking theory. Methodology: Methodologically, document analysis was used in the study. In the 3rd grade Life Science textbook, the main approach in the data on text contents, activity questions and statements are that the statements have both a philosophical and critical meaning. The relevant data were analyzed according to the categories in Lipman's critical thinking approach and presented in tables. Findings: The findings revealed that the questions and statements that meet Lipman's critical thinking categories were insufficient. While the expressions among the categories were predominantly judgment formation and being criterion/measure based, the least common categories were context sensitivity and self-correction. Highlights: In this context, it is essential to consider the developmental stages of primary education students and contemporary educational requirements, while integrating a philosophical critical thinking approach more thoroughly into the curriculum and practices to foster an active and inquisitive student personality.

Strong coupling induced ultrasensitive chiral detection by metal-dielectric hybrid metasurface

Circular dichroism (CD) spectroscopy struggles to detect weak signals from small, chiral molecules due to limitations in light absorption. This study introduces a method for significantly enhancing CD signals using a metal-dielectric hybrid metasurface. The metasurface creates a superchiral hotspot that amplifies the chirality of nearby molecules. Two techniques are proposed to overcome signal cancellation between the intrinsic and induced signals to achieve an overall enhancement of the CD signal by up to 23,630 times. This approach has the potential for ultrasensitive detection of chiral molecules at the nanoscale, with applications in pharmacology, toxicology, and life sciences.

First report of tomato ringspot virus infection of Krymsk 86 (Prunus cerasifera × P. persica), a hybrid rootstock, in California.

California produces 99% of prunes (Prunus domestica) in the U.S.A., valued at $148 million and accounting for 40% of world prune production. In the last decade, the rootstock Krymsk 86 (K86; P. cerasifera x P. persica) is increasingly used in prune production because of its high vigor, excellent anchorage and graft compatibility with a wide variety of Prunus crops. An estimated >50% of new prune plantings in the Sacramento Valley are on K86. In late spring of 2023, 'Improved French' prune trees in two Northern California counties, grafted on K86 and Lovell (P. persica) rootstocks, were declining with a pale colored canopy. One orchard was ~ 9-years-old and in the other, trees varied from ~ 20 years to newly planted. The percentage of affected trees in one orchard was 3.6% (n=1,824) and 4.6% (n=1,295) in trees on K86 and Lovell, respectively. Symptomatic trees were scattered throughout this orchard, with a higher density of affected trees in the northeast quadrant. Examination of the trunk revealed a necrotic brown line at the graft union, typical of prune brown line (PBL) disease (Mircetich and Hoy, 1981), caused by infection of rootstock by tomato ringspot virus (ToRSV), a member species of Nepovirus lycopersici (family Secoviridae. The virus is vectored by dagger nematodes (Teliz et al. 1967) and its presence was confirmed in one of the orchards. To confirm infection by ToRSV in declining trees, total RNA from leaf samples, feeder roots, and cambial tissue from bark samples from scion and rootstock of one tree each on K86 and Lovell, was obtained using RNeasy Plant Mini kit (www.qiagen.com) and tested by one step reverse transcription-polymerase chain reaction (RT-PCR) assay using primers previously described (Tang et al., 2014). The reaction conditions included RT at 54oC using random hexamers and Superscript III (Thermo Fisher Scientific, USA), followed by a denaturation step at 95°C for 5 min, and 35 amplification cycles of 95°C for 30 s, 55°C for 30 s, and 72°C for 45 sec; and a final extension of 72°C for 5 min. The expected size amplicons (176 bp) were visualized by agarose gel electrophoresis only from RNA obtained from the cambial tissue below the graft union confirming the ToRSV infection in K86 rootstock. Extracts from bark scrapings of the rootstocks of three symptomatic trees also tested positive for ToRSV with immunostrips (www.agdia.com). In subsequent RT-PCR tests, RNA extracts from cambial tissue from 9 of 10 symptomatic trees on K86 tested positive for ToRSV. The amplicons were purified using a gel extraction kit (Qiagen Inc., Valencia, CA), and subjected to Sanger sequencing (Azenta Life Sciences (South Plainfield, NJ, USA). The sequence of the ToRSV isolates 316 (GenBank accession PQ282959) and 318 (GenBank accession PQ2829560 exhibited 99.4% (175/176 base pairs) and 98.9 % (175/177) sequence identity, respectively, with ToRSV isolate Rasp1-2014 segment RNA2 (GenBank accession KM083895). Our results indicate that ToRSV can infect K86 and cause PBL. ToRSV and its nematode vectors have a wide host range that includes several crop plants and weeds, but this virus is not currently part of registration and phytosanitory certification of Prunus species in the state of California. This is the first report of ToRSV infection of the K86 rootstock. This information is important for the selection of a rootstock in prune orchards where the virus is endemic.

How to introduce a new bacteriophage on the block: a short guide to phage classification.

Bacteriophage (phage) studies established the field of molecular biology and continue to propel life science research forward due to their diversity, abundance, and potential applications. In this Gem article, we orient newcomers to four common ways phages are currently classified: infection cycle, morphology, taxonomy, and supergroup. By using these classifications, researchers can determine where any novel phage fits into the scheme of the known "phage-verse".

The coverage of basic and applied research in press releases on EurekAlert!

Research organisations routinely reach out to the media via press releases to announce research news and promote advances in science. This paper explores the presence of basic and applied research in press releases issued through EurekAlert!. Using a scientometric approach to classify research papers featured in press releases into basic and applied research, we found that more than half of the press releases in our dataset were related to basic research. This trend was particularly notable in life and earth sciences, physical sciences, and engineering fields. In contrast, press releases in the biomedical and health sciences, as well as in social sciences and humanities, were more frequently associated with applied research. Additionally, we present findings on the similarity and readability of press releases compared to their corresponding research papers, which confirm the role of institutional press officers in making research papers more accessible to the public and media. This adaptation appears to be more pronounced for basic research.

Biological Theories of Morphogenesis Based on Holistic Biophysical Thinking

AbstractThe roles played by physics in the study of the life sciences have taken many forms over the past 100 years. Here we analyze how physics can be brought to bear on the contemporary study of morphogenesis, where new tissue-scale forms arise out of simpler, more homogenous, initial structures. We characterize how morphogenesis has been studied through reductionist approaches and discuss their limitations. We suggest that an alternative way of approaching morphogenesis that begins with a consideration of the whole may also serve as a fruitful mode of scientific inquiry. Through historical analysis of concepts relevant for contemporary systems biology, we illustrate how physical thinking oriented toward the biological whole (“holistic biophysical thinking”) was exemplified in the biological theories presented earlier by D’Arcy Thompson (1860–1948) and Pere Alberch (1954–1998). We contrast the use of physics to develop the conceptual foundations of holistically oriented systems biology with the more prevalent contemporary use of physics that focuses on technological development and quantification, embedded within reductionist strategies. As an in-depth contemporary case study, we describe how in our research we have used holistic biophysical thinking to develop biological theories of vertebrate morphogenesis that account for and extend beyond genetic, molecular, and cellular processes. We propose a theory of skin development where both molecular and morphological patterns are orchestrated by emergent physical processes at the supracellular scale. We further posit that holistic biophysical thinking at the supracellular scale may advance theories of diseases such as cancer.

High Fluorescence of Phytochromes Does Not Require Chromophore Protonation

Fluorescing proteins emitting in the near-infrared region are of high importance in various fields of biomedicine and applied life sciences. Promising candidates are phytochromes that can be engineered to a small size and genetically attached to a target system for in vivo monitoring. Here, we have investigated two of these minimal single-domain phytochromes, miRFP670nano3 and miRFP718nano, aiming at a better understanding of the structural parameters that control the fluorescence properties of the covalently bound biliverdin (BV) chromophore. On the basis of resonance Raman and time-resolved fluorescence spectroscopy, it is shown that in both proteins, BV is deprotonated at one of the inner pyrrole rings (B or C). This protonation pattern, which is unusual for tetrapyrroles in proteins, implies an equilibrium between a B- and C-protonated tautomer. The dynamics of the equilibrium are slow compared to the fluorescence lifetime in miRFP670nano3 but much faster in miRFP718nano, both in the ground and excited states. The different rates of proton exchange are most likely due to the different structural dynamics of the more rigid and more flexible chromophore in miRFP670nano3 and miRFP718nano, respectively. We suggest that these structural properties account for the quite different fluorescent quantum yields of both proteins.