The ANN with back propagation algorithm is a multi-layer feed-forward neural network, which is suitable to study unsteady frost formation with multiple factors. The back propagation ANN algorithm is used to study frost layer growth on cold flat surface, where four feature variables including temperature of cold flat surface, the velocity, relative humidity, and temperature of air are adopted. The frost growth experiment generates the database, which is good for training frost growth due to its fast speed and high precision based on Levenberg-Marquardt learning rule. The establishment of neural network model in this paper can quickly and accurately predict the frost layer height on cold flat surface of different control variables, which is helpful for the implementation of defrosting.