Blood Serotonin Levels Research Articles

Clinical, developmental and serotonemia phenotyping of a sample of 70 Italian patients with Phelan-McDermid Syndrome

BackgroundPhelan-McDermid syndrome (PMS) is caused by monoallelic loss or inactivation at the SHANK3 gene, located in human chr 22q13.33, and is often associated with Autism Spectrum Disorder (ASD).ObjectivesTo assess the clinical and developmental phenotype in a novel sample of PMS patients, including for the first time auxometric trajectories and serotonin blood levels.Methods70 Italian PMS patients were clinically characterized by parental report, direct medical observation, and a thorough medical and psychodiagnostic protocol. Serotonin levels were measured in platelet-rich plasma by HPLC.ResultsOur sample includes 59 (84.3%) cases with chr. 22q13 terminal deletion, 5 (7.1%) disruptive SHANK3 mutations, and 6 (8.6%) ring chromosome 22. Intellectual disability was present in 69 (98.6%) cases, motor coordination disorder in 65 (92.9%), ASD in 20 (28.6%), and lifetime bipolar disorder in 12 (17.1%). Prenatal and postnatal complications were frequent (22.9%-48.6%). Expressive and receptive language were absent in 49 (70.0%) and 19 (27.1%) cases, respectively. Decreased pain sensitivity was reported in 56 (80.0%), hyperactivity in 49 (80.3%), abnormal sleep in 45 (64.3%), congenital dysmorphisms in 35 (58.3%), chronic stool abnormalities and especially constipation in 29 (41.4%). Parents reported noticing behavioral abnormalities during early childhood immediately after an infective episode in 34 (48.6%) patients. Brain MRI anomalies were observed in 53 (79.1%), EEG abnormalities in 16 (23.5%), kidney and upper urinary tract malformations in 18 (28.1%). Two novel phenotypes emerged: (a) a subgroup of 12/44 (27.3%) PMS patients displays smaller head size at enrollment (mean age 11.8 yrs) compared to their first year of neonatal life, documenting a deceleration of head growth (p < 0.001); (b) serotonin blood levels are significantly lower in 21 PMS patients compared to their 21 unaffected siblings (P < 0.05), and to 432 idiopathic ASD cases (p < 0.001).ConclusionsWe replicate and extend the description of many phenotypic characteristics present in PMS, and report two novel features: (1) growth trajectories are variable and head growth appears to slow down during childhood in some PMS patients; (2) serotonin blood levels are decreased in PMS, and not increased as frequently occurs in ASD. Further investigations of these novel features are under way.

A Systematic Review on Autism and Hyperserotonemia: State-of-the-Art, Limitations, and Future Directions.

Hyperserotonemia is one of the most studied endophenotypes in autism spectrum disorder (ASD), but there are still no unequivocal results about its causes or biological and behavioral outcomes. This systematic review summarizes the studies investigating the relationship between blood serotonin (5-HT) levels and ASD, comparing diagnostic tools, analytical methods, and clinical outcomes. A literature search on peripheral 5-HT levels and ASD was conducted. In total, 1104 publications were screened, of which 113 entered the present systematic review. Of these, 59 articles reported hyperserotonemia in subjects with ASD, and 26 presented correlations between 5-HT levels and ASD-core clinical outcomes. The 5-HT levels are increased in about half, and correlations between hyperserotonemia and clinical outcomes are detected in a quarter of the studies. The present research highlights a large amount of heterogeneity in this field, ranging from the characterization of ASD and control groups to diagnostic and clinical assessments, from blood sampling procedures to analytical methods, allowing us to delineate critical topics for future studies.

Evaluation of serotonin levels in full-term newborns of diabetic mothers

BACKGROUND: The growth of neurological and mental diseases in the offspring of patients with pre- and gestational diabetes mellitus determines the need to study the regulatory function of the serotoninergic system of the brain in newborns. This plays a key role in its morphofunctional development in early ontogenesis, which is necessary for timely diagnosis of disorders and prevention of long-term consequences.&#x0D; AIM: The aim of this study was to evaluate serotonin levels in full-term newborns with diabetic fetopathy from mothers with pre- and gestational diabetes mellitus.&#x0D; MATERIALS AND METHODS: The main group consisted of 45 newborns with diabetic fetopathy, of whom 30 individuals were from mothers with type 1 diabetes mellitus and 15 ones from mothers with gestational diabetes mellitus. The control group comprised 20 healthy full-term newborns from healthy mothers without pregnancy complications. Serotonin concentrations were determined in platelet-rich plasma of blood from the umbilical cord vein, and in a platelet suspension prepared from venous blood taken on the first day of life, using high-performance liquid chromatography with electrochemical detection.&#x0D; RESULTS: Platelet-rich plasma serotonin level in umbilical cord blood taken from newborns of the main group was more than two times lower compared to children of healthy mothers. This parameter in venous blood taken from mothers with type 1 diabetes (0.744 ± 0.117 µmol/l) corresponded to that in healthy patients, while in mothers with gestational diabetes mellitus, it was significantly lower and amounted to 0.331 ± 0.071 µmol/l (p 0.05). Moreover, platelet-rich plasma serotonin level in all newborns correlated with that in their mothers (R = 0.505; p 0.05). Serotonin levels in venous blood platelets of newborns of the main group was almost 2.5 times lower than in healthy ones.&#x0D; CONCLUSIONS: The data obtained indicate the need to use platelet serotonin values as a biochemical marker of disorders of functional brain development in children with diabetic fetopathy.

The impact of 5-hydroxytryptophan supplementation on sleep quality and gut microbiota composition in older adults: A randomized controlled trial

Background and AimsSleep quality is a pivotal part of health and there is growing evidence on the association between gut microbiota composition and sleep quality. 5-Hydroxytryptophan (5-HTP) is known as a precursor of the sleep regulating neurotransmitter and hormone however, efficacy of 5-HTP supplementation for improving sleep quality in older adults is unclear. Hence, the aim of this study is to assess the impact of 5-HTP supplementation on sleep quality and gut microbiota composition in older adults. MethodsThis is a single-blinded, 12-week parallel randomized controlled. Thirty older adults (66 ± 3 years) in Singapore were randomly assigned to either consume or not consume 100 mg 5-HTP daily. Every 4 weeks, sleep quality was assessed via both subjective (Pittsburg Sleep Quality Index) and objective (actigraphy watch) measures. A global sleep score (GSS) was obtained from the PSQI, where a GSS>5 defines as poor sleeper while a GSS≤5 defines as good sleeper. Blood serotonin level, urine melatonin concentration, gut microbiota composition and stool short chain fatty acids (SCFA) content was assessed at week 0 and 12. This study was registered in clinicaltrials.gov as NCT04078724 (https://clinicaltrials.gov/ct2/show/NCT04078724) Results5-HTP supplementation showed an overall favorable effect on certain sleep quality components and an increase in serum serotonin concentration. In particular, at week 12, not good sleepers but poor sleepers with 5-HTP supplementation were able to significantly improve subjective GSS (ΔSL5-HTP: -2.80 ± 1.10 mins, p-value=0.005). In addition, they showed an increase in microbiota diversity (Simpson5-HTP vs. SimpsonControl: 0.037 ± 0.032 a.u. vs. -0.007 ± 0.022 a.u.; pinteraction: 0.013) and relative abundance of SCFA producing bacteria in the gut. Conclusions5-HTP supplementation can improve certain sleep quality components in older adults and this benefit was more prominently observed in poor sleepers. 5-HTP was also able to improve the gut microbiota composition in poor sleepers.

SEROTONIN INFLUENCE ON THE CLINICAL COURSE OF PREGNANCY IN THE FIRST TREMESTER

Changes of melatonin and serotonin levels in the plasma can be associated with thedevelopment of the threatened abortion. Hormones of the pineal gland stimulate thesecretion of progesterone, which reduces the contractile activity of the uterus and preventsthe immunological rejection of the trophoblast. The real observations motivated us tostudy serotonin plasma concentrations in cases of the threatened abortion.The aim of the study – to study changes in serotonin levels in pregnant women withclinical manifestations of the threatened abortion before 12 weeks of gestation comparedto women with normal pregnancies.Research material and methods. Two groups were formed: the 1st main group –40 pregnant women with signs of threatened abortion, the 2nd control group – 44 healthypregnant women. An obstetric study, enzyme immunoassay to determine serotonin, andultrasound were carried out. Statistical analysis was conducted according to the generallyaccepted methods of variational statistics.The approval of the scientific work was obtained from the Bioethical Commission of theBukovinian State Medical University (Ukraine).Research results. Results of serotonin levels in venous blood showed their extremeincrease in women with threatened abortion 739.89±111.27 ng/ml compared to normalpregnancy 511.05±140.36 ng/ml (p˂ 0.003). In the pregnant women of the main group,consisting of 40 women, almost all of them had an increased serotonin, except 4 women(10%). This can be explained by hypoprogesteronemia in women with a threat of abortion.Therefore, we consider the change in serotonin concentration as a secondary process thatdoes not require specific treatment. Attention is also drawn to the fact of a reduced levelof serotonin in the control group in cases of further complicated course. Conclusions. Serotonin levels were higher in patients with threatened abortion comparedto uncomplicated pregnancies. Changes in serotonin plasma in cases of threatenedabortion are a consequence, not a cause of abortion, therefore pregnant women needtreatment of obstetric pathology, namely the prescription of progesterone.

An exploratory study on the association between serotonin and sleep breathing disorders

This exploratory observational study aimed to evaluate whether the blood levels of serotonin and enzymes involved in serotonin synthesis are associated with sleep breathing parameters. A total of 105 patients were included in this study, who were subjected to single-night polysomnography with simultaneous audio–video recordings. Peripheral blood samples were collected to estimate the serum levels of serotonin, tryptophan hydroxylase 1 (TPH1), and aromatic l-amino acid decarboxylase (AADC). Results showed a negative correlation between blood serotonin levels, and oxygen desaturation index (ODI) (p = 0.027), central apnea (p = 0.044) and obstructive apnea (OA) (p = 0.032) scores. Blood TPH1 levels were negatively correlated with average (p = 0.003) and minimal saturation (p = 0.035) and positively correlated with apnea–hypopnea index (p = 0.010), OA (p = 0.049), and hypopnea index (p = 0.007) scores. A tendency to sleep-disordered breathing seemed to co-occur with lower blood serotonin and higher TPH1 levels.Clinical Trial Registration :www.ClinicalTrials.gov, identifier NCT04214561

Unlocking the Secrets: Exploring the Biochemical Correlates of Suicidal Thoughts and Behaviors in Adults with Autism Spectrum Conditions.

Involving 1 million people a year, suicide represents one of the major topics of psychiatric research. Despite the focus in recent years on neurobiological underpinnings, understanding and predicting suicide remains a challenge. Many sociodemographical risk factors and prognostic markers have been proposed but they have poor predictive accuracy. Biomarkers can provide essential information acting as predictive indicators, providing proof of treatment response and proposing potential targets while offering more assurance than psychological measures. In this framework, the aim of this study is to open the way in this field and evaluate the correlation between blood levels of serotonin, brain derived neurotrophic factor, tryptophan and its metabolites, IL-6 and homocysteine levels and suicidality. Blood samples were taken from 24 adults with autism, their first-degree relatives, and 24 controls. Biochemical parameters were measured with enzyme-linked immunosorbent assays. Suicidality was measured through selected items of the MOODS-SR. Here we confirm the link between suicidality and autism and provide more evidence regarding the association of suicidality with increased homocysteine (0.278) and IL-6 (0.487) levels and decreased tryptophan (-0.132) and kynurenic acid (-0.253) ones. Our results suggest a possible transnosographic association between these biochemical parameters and increased suicide risk.

Neuroprotective Effect of White Nelumbo nucifera Gaertn. Petal Tea in Rats Poisoned with Mancozeb.

Nelumbo nucifera Gaertn. (N. nucifera) tea is used as food and folk medicine to reduce toxicity in Southeast Asia. Mancozeb (Mz) is used for controlling fungi in agriculture and contains heavy metals. This study aimed to examine the effect of white N. nucifera petal tea on cognitive behavior, hippocampus histology, oxidative stress, and amino acid metabolism in rats poisoned with mancozeb. Seventy-two male Wistar rats were divided into nine groups (n = 8 in each). Y-maze spontaneous alternation test was used to assess cognitive behavior, and amino acid metabolism was investigated by nuclear magnetic resonance spectroscopy (1H-NMR) from blood. There was a significant increase in relative brain weight in the Mz co-administered with the highest dose (2.20 mg/kg bw) of white N. nucifera group. The levels of tryptophan, kynurenine, picolinic acid, and serotonin in blood showed a significant decrease in the Mz group and a significant increase in the Mz co-administered with low dose (0.55 mg/kg bw) of white N. nucifera group. However, there was no significant difference in cognitive behavior, hippocampus histology, oxidative stress, and corticosterone. This study demonstrated that a low dose of white N. nucifera petal tea has a neuroprotective effect against mancozeb.

Bronchial Typical Carcinoid with Carcinoid Syndrome Successfully Treated with Everolimus and Octreotide: a Case Report

Background. Bronchopulmonary typical carcinoid tumors with distant metastasis and carcinoid syndrome are rare. Case. We report the case of a 77-year-old woman diagnosed with a bronchial typical carcinoid in the right middle lobe bronchus and multiple liver metastases. The patient presented with facial flushing and diarrhea, and the serotonin blood levels were elevated, indicative of carcinoid syndrome. Combination therapy with everolimus and octreotide maintained the patient in a healthy condition for approximately two years until she died in 2019. Conclusion. Bronchial typical carcinoids with multiple liver metastases and carcinoid syndrome in our patient was well-managed by combination therapy with everolimus and long-acting release octreotide.

Effectiveness of Motivational Counseling for Lifestyle Modification in Obese Patients Using a Patient-centered Approach

Obesity is a predictor of a significant number of non-communicable diseases. Reduction of body weight by 5–10 % in 6 months, has a positive effect on the prognosis of life and is one of the goals of treatment. To achieve such goals, there is a need for a patient-oriented approach, where the degree of individualization corresponds to the personal risks and needs of the patient with his involvement in the process of choosing a treatment strategy and forming new flexible life habits through the use of motivational counseling. The objective: is to determine the effectiveness of motivational counseling for lifestyle modification in obese patients of prime working age using a patient-oriented approach. Materials and methods. 37 patients with obesity I (27 people) and II (10 people) degree, aged 38.03±1.27 years (20 women and 17 men) were examined. Patients were given motivational counseling according to the «5 As» system, the patient-oriented approach included recommendations regarding the regime and caloric content of food, physical activity (monitoring with a pedometer), compliance with sleep hygiene, correction of psycho-emotional disorders. Clinical laboratory examination in dynamics after 1 month, 3 months and 6 months included measurements of BMI, waist and hip circumference, body surface area, waist/hip ratio, conicity index, body shape index, abdominal volume index, blood pressure, levels of blood glucose, insulin, HOMA index, cholesterol, lipidogram indicators, serotonin and leptin. In addition, surveys were conducted using the HADS hospital anxiety and depression scale, Beck’s scale, Hamilton scale, Dutch Eating Behavior Questionnaire, Epworth Sleepiness Scale, Pittsburgh Sleep Quality Questionnaire, SF-36, The International Physical Activity Questionnaire, The Finnish Diabetes Risk Score. Statistical analysis was performed using IBM SPSS Statistics, Statistica 12, descriptive statistics Excel 2010. Results. During 6 months of observation, patients lost more than 5 % of their body weight (p&lt;0.05), which was accompanied by a significant decrease in BMI (p&lt;0.01) and an increase in the level of physical activity (p&lt;0.001). The levels of lipid and carbohydrate metabolism exceeded the recommended values and had a positive tendency to decrease during treatment (p&gt;0.05). Assessments of the manifestations of anxiety and depression according to the HADS depression and Hamilton scales, eating disorders, serotonin and leptin levels, quality of sleep, quality of life of patients improved. The prediction of the risk of developing diabetes had significant positive dynamics (p&lt;0.05). Conclusions. The obtained results make prove that the application of of motivational counseling according to the step-bystep system «5 As» with a patient-oriented approach in patients of working age with obesity is an effective method, which is confirmed by reliable positive results and allows to reduce body weight by more than 5 % in 6 months, reduce levels of metabolic disorders, blood pressure, increase physical activity, reduce the manifestations of depression and anxiety, eating disorders, improve the quality of sleep and life of patients, improve the prognosis and reduce the risk of non-infectious diseases. If it is necessary to achieve stricter targets of indicators in obese patients and/or in a shorter time, it is necessary to carry out a further search for effective measures and consider the additional use of pharmaceutical products in prevention.

Serotonin levels in adolescents with obesity and non­alcoholic fatty liver disease

The prevalence of obesity has reached epidemic proportions not only among adults, but also in the pediatric population. Expectedly the number of obesity-associated diseases, including non­alcoholic fatty liver disease (NAFLD) etc., increases, too. The pathogenesis of these conditions has common links, some of them are well known, others are being investigated. The latter include hormones of the gastrointestinal tract. They are considered as a part of the body’s humoral regulation. These compounds regulate energy balance of a body, and insulin resistance. It is assumed they can affect obesity and NAFLD pathogenesis.&#x0D; Objective — to determine serotonin levels in adolescents with obesity and NAFLD.&#x0D; Materials and methods. An examination of 108 adolescents aged 12—17 years with obesity (55 boys and 53 girls) was carried out. The control group consisted of 32 healthy adolescents (18 boys and 14 girls). Depending on the hepatobiliary pathology all patients were divided into two groups: 1st group included 29 (26.9 %) adolescents with functional disorders of the biliary tract (FDBT), and 2nd group consisted of 79 patients (73.1 %) with NAFLD and FDBT.All patients underwent a comprehensive clinical and anamnestic examination, clinical and biochemical blood tests, including determination of the levels of γ-glutamyl transpeptidase (GGT), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (AP), total bilirubin and its fractions, triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), very low-density lipoprotein cholesterol (VLDL-C), atherogenic coefficient (AC) value. Levels of fasting glucose and immunoreactive insulin (IRI), HOMA-IR index, and blood serotonin level were also determined. An ultrasound examination of the abdominal cavity was performed.&#x0D; Results and discussion. According to the clinical and anamnestic data, there were no differences between the groups of adolescents with obesity, depending on the existing hepatobiliary pathology, except for a higher value of the WC/Height in patients with NAFLD and FDBT (p &lt; 0.01). The results of laboratory investigations demonstrated that in patients with NAFLD and FDBT, compared to patients with FDBT, the following parameters were higher: fasting glucose level (p &lt; 0.05), IRI (p &lt; 0.01), the value of the HOMA-IR index and the frequency of its increase (p &lt; 0.01), levels of ALT and AST activity (p &lt; 0.05), the levels of triglycerides and VLDL-­C (p &lt; 0.05). The level of serotonin was higher in the group of patients with NAFLD and FDBT compared to the control group (p &lt; 0.015).&#x0D; Conclusions. Adolescents with obesity, accompanied by NAFLD and FDBT, had higher rates characterizing abdominal obesity, carbohydrate and lipid metabolism, liver enzyme activity compared to patients with FDBT. Serotonin levels were higher in the group of patients with NAFLD and FDBT compared to the control group.

Sex-Specific Effects of Blood Serotonin on Reproductive Effort in a Small Passerine.

AbstractLaboratory animal models have shown that blood serotonin levels reflect consistent individual differences in behavioral decision-making and maternal behavior. Serotonin could also help to understand intraspecific variation in reproductive strategies, although the mechanisms are poorly understood. In this study, the relationships of plasma serotonin with breeding parameters and parental behavior were examined in wild great tits (Parus major). Females who laid eggs earlier had higher levels of serotonin in the second half of the nestling period, while no significant relationship of serotonin with clutch size, brood size, and body size was detected. In males, serotonin levels were negatively related to clutch size and brood size and positively related to body size. The association of serotonin with provisioning behavior was sex specific, and acute fear stress induced by a predator presentation did not change this relationship. Food provisioning was positively related to size-corrected serotonin levels in females and negatively related to size-corrected serotonin levels in males. These results suggest that peripheral serotonin is a sensitive marker of parental behavior and reproductive effort in wild birds, while the mechanisms linking this neurotransmitter to reproduction are probably mediated by interplay between the serotonergic system, sex hormones, and other neurotransmitters.

Genetically predicted circulating levels of glycine, glutamate, and serotonin in relation to the risks of three major neurodegenerative diseases: A Mendelian randomization analysis.

It has been previously postulated that blood neurotransmitters might affect risks of neurodegenerative diseases. Here, a Mendelian Randomization (MR) study was conducted to explore whether genetically predicted concentrations of glycine, glutamate and serotonin were associated with risks of Alzheimer’s disease (AD), Parkinson’s disease (PD), and amyotrophic lateral sclerosis (ALS). From three genome-wide association studies of European ancestry, single nucleotide polymorphisms strongly associated with glycine, glutamate and serotonin were selected as genetic instrumental variables. Corresponding summary statistics were also obtained from the latest genome-wide association meta-analyses of AD, PD and ALS. The inverse-variance weighted MR and multiple sensitivity analyses were performed to evaluate causal effects of genetically predicted levels of neurotransmitters on risks of neurodegenerative diseases. The statistical significance threshold was set at P < 0.0056 using the Bonferroni-correction, while 0.0056 < P < 0.05 was considered suggestive evidence for a causal association. There was a causal association of elevated blood glutamate levels with higher AD risks. The odds ratio (OR) of AD was 1.311 [95% confidence interval (CI), 1.087–1.580; P = 0.004] per one standard deviation increase in genetically predicted glutamate concentrations. There was suggestive evidence in support of a protective effect of blood serotonin on AD (OR = 0.607; 95% CI, 0.396–0.932; P = 0.022). Genetically predicted glycine levels were not associated with the risk of AD (OR = 1.145; 95% CI, 0.939–1.396; P = 0.180). Besides, MR analyses indicated no causal roles of three blood neurotransmitters in PD or ALS. In conclusion, the MR study provided evidence supporting the association of elevated blood glutamate levels with higher AD risks and the association of increased blood serotonin levels with lower AD risks. Triangulating evidence across further study designs is still warranted to elucidate the role of blood neurotransmitters in risks of neurodegenerative diseases.

Climatic and chemical health risk factors for people living in arctic and subarctic regions: population and sub-population levels

The article dwells on climatic and chemical risk factors that influence health of people living in the RF arctic and sub-arctic regions on population and sub-population levels. We used a model describing cause-effect relations between environmental factors and life expectancy at birth based on an artificial neural network to predict a future medical and demographic situation in territories with arctic and sub-arctic climate in the RF. Children’s health was examined profoundly due to a participating representative sampling. We comparatively analyzed clinical, biochemical and general clinical indicators in the test and reference groups using standard statistical procedures and statistical software packages. We established that average monthly temperatures in July grew on average by 3.4 % over 2010–2019 on the examined territories in the RF; precipitations in January and July grew by 13.0–15.1 %. The article presents differentiated estimates of emerging influence on life expectancy at birth (LEB) exerted by weather and climatic conditions on the analyzed territories with arctic and sub-arctic climate. Losses in LEB vary from 164 days in Yakutia to 349 days in Chukotka. Aggregated influence of weather and climatic factors in the arctic and sub-arctic zones in 2010–2019 produced variable effects on LEB, starting from negative ones that resulted in its decline in the Magadan region, the Nenets Autonomous Area, Chukotka, and the Yamal-Nenets Autonomous Area (-254; -211; -109 and -8 days accordingly) and to positive ones that led to the growth in LEB by up to 111 days in Yakutia. Children who are simultaneously exposed to adverse weather and climatic factors in the sub-arctic zone and substantial chemical pollution in ambient air have more frequent and more apparent negative changes in their health indicators in comparison with children from the reference group. Thus, respiratory diseases and diseases of the nervous system were by 5.6 times more frequent in the test group; levels of leukocytes, ESR, TSH, Apo-B and Apo-B/ApoA1 in blood were by 1.3–1.7 times higher, р = 0.0001. Levels of Apo A1, hydrocortisone, and serotonin in blood were by 1.2–2.5 times lower, р = 0.0001–0.040, etc. A share contribution made by chemical factors to associated respiratory diseases and diseases of the nervous system amounted to 25–31 %; adverse climatic factors, 10–15 % .

Климатические и химические факторы риска здоровью населения регионов арктической и субарктической зон: популяционный и субпопуляционный уровни

The article dwells on climatic and chemical risk factors that influence health of people living in the RF arctic and sub-arctic regions on population and sub-population levels. We used a model describing cause-effect relations between environmental factors and life expectancy at birth based on an artificial neural network to predict a future medical and demographic situation in territories with arctic and sub-arctic climate in the RF. Children’s health was examined profoundly due to a participating representative sampling. We comparatively analyzed clinical, biochemical and general clinical indicators in the test and reference groups using standard statistical procedures and statistical software packages. We established that average monthly temperatures in July grew on average by 3.4 % over 2010–2019 on the examined territories in the RF; precipitations in January and July grew by 13.0–15.1 %. The article presents differentiated estimates of emerging influence on life expectancy at birth (LEB) exerted by weather and climatic conditions on the analyzed territories with arctic and sub-arctic climate. Losses in LEB vary from 164 days in Yakutia to 349 days in Chukotka. Aggregated influence of weather and climatic factors in the arctic and sub-arctic zones in 2010–2019 produced variable effects on LEB, starting from negative ones that resulted in its decline in the Magadan region, the Nenets Autonomous Area, Chukotka, and the Yamal-Nenets Autonomous Area (-254; -211; -109 and -8 days accordingly) and to positive ones that led to the growth in LEB by up to 111 days in Yakutia. Children who are simultaneously exposed to adverse weather and climatic factors in the sub-arctic zone and substantial chemical pollution in ambient air have more frequent and more apparent negative changes in their health indicators in comparison with children from the reference group. Thus, respiratory diseases and diseases of the nervous system were by 5.6 times more frequent in the test group; levels of leukocytes, ESR, TSH, Apo-B and Apo-B/ApoA1 in blood were by 1.3–1.7 times higher, р = 0.0001. Levels of Apo A1, hydrocortisone, and serotonin in blood were by 1.2–2.5 times lower, р = 0.0001–0.040, etc. A share contribution made by chemical factors to associated respiratory diseases and diseases of the nervous system amounted to 25–31 %; adverse climatic factors, 10–15 %

Traumatic brain injury alters the gut-derived serotonergic system and associated peripheral organs

Most efforts to understand the pathology of traumatic brain injury (TBI) have been centered on the brain, ignoring the role played by systemic physiology. Gut-derived serotonin is emerging as a major regulator of systemic homeostasis involving various organs and tissues throughout the body. Here, we shed light on the roles occupied by gut-derived serotonin and its downstream metabolic targets in the systemic pathogenesis of TBI. Male C57BL/6J mice were subjected to a fluid percussion injury (FPI) and RT-qPCR was used to examine mRNA levels in intestine, liver, and adipose tissue. In the intestinal tract, TBI transiently downregulated enteric neuronal markers Chat and Nos1 in the duodenum and colon, and altered colonic genes related to synthesis and degradation of serotonin, favoring an overall serotonin downregulation. There also was a decrease in serotonin fluorescence intensity in the colonic mucosa and reduced circulating blood serotonin levels, with concurrent alterations in serotonin-associated gene expression in downstream tissues after TBI (i.e., upregulation of serotonin receptor Htr2a and dysregulation of genes associated with lipid metabolism in liver and adipose). Levels of commensal bacterial species were also altered in the gut and were associated with TBI-mediated changes in the colonic serotonin system. Our findings suggest that TBI alters peripheral serotonin homeostasis, which in turn may impact gastrointestinal function, gut microbiota, and systemic energy balance. These data highlight the importance of building an integrative view of the role of systemic physiology in TBI pathogenesis to assist in the development of effective TBI treatments.

Possible relations between arterial hypertension and cervical spine fibromyalgias (literature review)

The literature review analyses and systematises currently available information on fi-bromyalgia etiology and pathogenesis, hypertension classification and causes, and considers pos-sible relationships between cervical fibromyalgia and hypertension. Statistical data on mortality in&#x0D; Ukraine related to cardiovascular diseases, including hypertension, are highlighted. A concise clas-sification of arterial hypertension according to the International Classification of Diseases-10 (ICD-10) and the International Classification of Diseases-11 (ICD-11) is provided, and its pathogenesis&#x0D; (emission hypertension, resistance hypertension and hypervolemia) is outlined. Physiological rela-tions between cervical spine and cardiovascular system are considered. The main diagnostic criteria&#x0D; for fibromyalgia classification and its development stages are described. Prevalence of fibromyalgiais identified and its forms are classified in accordance with ICD-11. The available data on the likelycauses of fibromyalgia pain and any established mechanisms of fibromyalgia pathogenesis, including,but not limited to peripheral and central sensitisation, neurogenic inflammatory processes occurringin the disease-associated peripheral tissues, spinal cord and brain, as well as potential involvementof genetic, endocrine, psychopathological factors and sleep disorders in fibromyalgia development&#x0D; are discussed. The current formal criteria for fibromyalgia classification (revised in 2016), togeth-er with assessment of the main pharmacological and non-pharmacological fibromyalgia treatment&#x0D; methods and approaches based upon available published clinical trials outcomes are described. The&#x0D; main directions of fibromyalgia research are identified, and further prospective studies covering dif-ferent relationship aspects between hypertension and cervical fibromyalgia are evaluated. Among&#x0D; the available literature sources, the only study of patients with comorbid fibromyalgia (fibromyalgiawas diagnosed using 1990 American College of Rheumatology criteria and evaluated by Revisedfibromyalgia impact questionnaire (FIQR)) and hypertension (blood pressure levels were assessedusing the WHO protocol) has been reported. However, this group has only studied the dynamics ofchanges in cortisol, melatonin and serotonin blood levels, which accompany these pathologies, andthese authors have found that fibromyalgia tender points’ quantity and FIQR scores are much higher&#x0D; in hypertensive patients with fibromyalgia in comparison with the rest, which has led them to the con-clusion that hypertension prevalence in patients with fibromyalgia could be related to fibromyalgia severity. Thus, this thorough literature review demonstrates that clinical studies examining possiblerelationships between hypertension and cervical spine fibromyalgia are at lack and require furtherconsiderations.

Intestinal Predictors of Whole Blood Serotonin Levels in Children With or Without Autism.

Hyperserotonemia, or elevated levels of whole blood serotonin (WB5-HT), was the first biomarker linked to autism spectrum disorder (ASD). Despite numerous studies investigating the etiology of hyperserotonemia, results have been inconsistent. Recent findings suggest a relationship between the immune system and hyperserotonemia. The current study investigated whether intestinal 5-HT levels, 5-HT gene expression, or intestinal cell types predict WB5-HT. Participants included thirty-one males aged 3-18 who were classified into one of three groups: ASD and functional GI issues, typically developing with GI issues, and typically developing without GI issues. Samples from a lower endoscopy were analyzed to examine the pathways in predicting WB-5HT. Results demonstrated an association between T-Lymphocytes and WB5-HT.

Analysis of the relationships between the content of serotonin and noradrenalinе in the peripheral blood and the suicidal behavior

Laboratory blood testing is one of the most promising methods for determining the suicidal risk biomakers because some neurotransmitters and neuropeptides such as norepinephrine and serotonin can penetrate through the bloodbrain barrier. Three groups were formed: the persons who used the highly lethal methods of self-harm (PUHMS); the persons who made a suicidal attempt in different ways (PSA); the persons who experienced psychosocial stress and previously did not commit suicide attempts (comparison group, GS). The content of serotonin and norepinephrine in blood plasma was determined by the enzyme immunoassay on a Stat Fax 2100 spectrophotometer. The depression severity was assessed using the Montgomery‒Asbrega scale, the motivation to commit suicide was assessed using an analog scale. The highest level of serotonin and noradrenaline was observed in the PSA; in half of this group (50 %), depression was not determined, the motivation severity was statistically significantly lower than in the PUHMS, but higher than in the GS where the noradrenaline content in the peripheral blood was significantly higher than in the PUHMS. The lowest norepinephrine level was noted in the GLIVS where the serotonin level was within the physiological norm (the mean value is 60.4 ng/ml, the physiological norm is 50–220 ng/ml). In the GS, the serotonin level was below the physiological norm (36 ng/ml), and the depression severity corresponded to the PUHMS indicators, however, in the PUHMS, persons with severe depressive disorder predominated (PUHMS – 23.1 %, GS –12.3 %). The low levels of serotonin and norepinephrine in the peripheral blood may indicate the risk of committing suicide.

Meso-Forte — Innovative Method for Musical-Acoustic Psychotherapy and Neurohormonal Correction with Anti-Aging Effect: Clinical Study

This article is devoted to Meso-Forte therapy (MFT) — the innovative scientific method of music therapy. In a long time of clinical studies, music therapy has identified various therapeutic and health-improving effects. Our team also studied regenerative possibilities of musical acoustic impacts on different cell cultures in vitro, including blood cells. Authors found an exciting phenomenon that specific acoustic parameters can intensify the activity of various cell cultures while others inhibit this activity. That data brought us to join healing factors in one technology. As a result, authors have developed MFT, which uses Digital algorithmic music therapy for stress-related mental disorders treatment and anti-aging; as well as local impacts of acoustic vibrations magnetic energy for skin rejuvenation. Aim. To learn the Meso-forte therapy effectiveness in treating stress-related neurosis with a concomitant skin disorder in the form of accelerated aging. Material and methods. The study involved 52 women aged 30 to 60 years with various signs of stress-related neurosis combined with accelerating face skin aging. All examined patients were randomly divided into two groups. The Leading group of 27 persons underwent MFT with the cosmetic masks: 10 procedures, 20–25 minutes each day. The Control group of 25 persons experienced listening to usual background music with the same cosmetic masks. The comprehensive research program included tests carried out before and after ten interventions. Psychological tests: WAM (Well-Being, Activity, Mood), Taylor’s anxiety questionnaire. Medical tests: enzyme immunoassay method to measure cortisol and serotonin blood levels, laser doppler flowmetry to study blood flow in subcutaneous vessels, an objective device assessment of the skin condition. Results and discussion. In the Leading group, authors found reliable signs of mental-emotional improvement, optimization of the hormonal system, skin rejuvenation with the augmentation of the subcutaneous blood flow. In the Control group, there were no significant dynamics. Conclusion. Clinical study shows that MFT provides complex effects on the organism. Due to the integration of science, art, medicine, and modern technology, authors managed to create an effective high-tech tool for psychotherapy, healing, and rejuvenation that will be helpful to a doctor of any specialty or a psychologist.