Levels Of Phosphorus Fertilization Research Articles

Assessing the impact of organic resources and phosphorus on nutrient uptake, soil traits and seed potato productivity

AbstractImproving tuber yield size to boost seed potato (Solanum tuberosum) production efficiency is a paramount goal, particularly in Punjab, India, a major contributor to the nation's seed supply. To optimize nutrient absorption, soil quality, and yield of seed‐sized tubers, research was conducted in Ludhiana and Jalandhar over two years. Employing a split‐plot design, three organic treatments (farmyard manure [FYM], biofertilizer, control) were tested in main plots, while five phosphorus fertilizer levels (46.9, 62.5, 93.8, 125, 0 kg ha−1 P2O5) were examined in subplots. Results revealed that FYM notably enhanced nitrogen, phosphorus, and potassium uptake in both haulm and tubers compared to other organics. Moreover, higher phosphorus levels, particularly 125 kg ha−1 P2O5, maximized nutrient uptake. Soil attributes like available nitrogen remained unaffected, whereas phosphorus availability increased with FYM and higher phosphorus levels. Available potassium remained consistent across treatments. Soil pH, electrical conductivity, and organic carbon were unchanged with 125 kg ha−1 P2O5 treatments. FYM and biofertilizer significantly increased total tuber yields, with FYM showing a remarkable 30% boost compared to the control, and 125 kg ha−1 P2O5 phosphorus levels resulting in a notable 24% increase, indicating enhanced seed potato production strategies. Principal component analysis underscored the positive correlation between tuber yield and soil attributes, particularly favoring FYM and 125 kg ha−1 P2O5 treatments. Overall, FYM application and optimal phosphorus fertilizer levels are pivotal for augmenting nutrient uptake, soil health, and yield in seed potato cultivation.

Effects of Sowing Dates and Phosphorus Application on Seed Yield and Yield Components of Alfalfa

Background: Alfalfa (Medicago sativa L.) is a globally important cool-season forage grass for semiarid regions and the demand for seeds is increasing with the increase of alfalfa planting area. However, its seed yield is often low or inconsistent. Methods: A field experiment was conducted to investigate the response of seed yield and yield components of alfalfa to different sowing dates and phosphorus (P) fertilization levels from 2020 to 2022. This research was conducted using a split-plot design in a randomized complete block, with four replications. The main plots were three sowing dates (20 July, 5 and 20 August) and subplots were five different rates (0, 40, 70, 100 and 130 kg P2O5 ha-1). Result: Our results revealed that delaying the sowing dates reduced seed yield, by influencing its yield components, except for the 1000-seed weight, but P fertilization enhanced the seed yield components affected by late sowing and increased seed yield, it is recommended to apply P fertilizer at 77.6~144.8 kg ha-1. Stem density and seeds per pod were the primary factors determining seed yield and they may be useful to consider in developing traits for high-seed-yield alfalfa.

Optimization of Crop Establishment Methods and Phosphorus Fertilizer Levels on Growth and Economic Efficiency of Groundnut under Semi-Arid Region of Afghanistan

An experiment was conducted at the farm of Afghanistan National Agricultural Science and Technology University (ANASTU), Kandahar Province, Afghanistan in cropping season of 2020 to investigate the Optimization of Crop Establishment methods and Phosphorus Fertilizer levels on Growth and Economic Efficiency of Groundnut under Semi-arid region of Afghanistan. The experimentation was conducted in split-plot design with 15 treatment combinations and replicated thrice. main-plot consisted of crop establishment methods, viz. ridge and furrow (RF), broad bed and furrow (BBF) and flatbed (FB), while the sub-plots comprised of phosphorus levels, viz. absolute control, 20, 40, 60 and 80 kg P2O5/ ha. The results of the investigation revealed that the growth parameters in terms of plant height in and leave area were highest in BBF, followed by FB and minimum was in RF. Adoption of BBF recorded significantly higher gross return (238928 AFN/ha), net return (202728 AFN/ha) and net benefit cost of ratio (5.2) were in Broad Bed and Furrow, followed in ridged and furrow and the minimum was in flat bed method. It can be concluded, cultivating of groundnut with Broad Bed and Furrow with application of 60 kg P2O5/ha was found beneficial for reaching higher productivity and profitability under semi-arid region of Afghanistan.

Effect of Potassium Humate, Phosphorus and Copper on Onion Quantitative and Qualitative Yield

Previous research has been limited regarding the combined impacts of copper and potassium humate under various phosphorus fertilization levels on onion crops. So, a field trials were performed during two successive winter seasons of 2021 - 2022 and 2022 - 2023 aiming to evaluate three levels of calcium superphosphate (representing 100 %, 75 % and 50 % of the phosphorus recommended dose) as main plot treatments, along with three levels of potassium humate soil addition ( 0.0 %, 1.0 % and 2.0 % ) as sub-plot treatments. Additionally, foliar spraying of copper was carried out at three different rates (0.0 %, 0.1 % and 0.2 %) as sub-subplot treatments. These factors were examined individually or in combination to assess their impact on onion performance, quantitative yield, and qualitative attributes. Firstly, in terms of the individual effect of phosphorus recommended dose (PRD), the application of 100% of PRD demonstrated superior impacts on onion growth performance, quantitative and qualitative yield compared to 75% and 50% of PRD, respectively. Secondly, focusing on the individual effect of soil addition of potassium humate, all parameters related to growth performance, quantitative and qualitative yield, such as bulb weight and diameter, neck diameter, marketable bulb yield, total dissolved solids (TDS), total sugar (%), carbohydrates, and crude protein, exhibited an increase with higher rates of added potassium humate. Thirdly, the values of all parameters studied increased as the rate of copper application increased, indicating a positive correlation between copper application and onion crop performance. Lastly, the combined treatment of 100% of PRD with 2.0% potassium humate and 0.2% copper recorded the highest values across all measured parameters. Additionally, the combined treatment of 75% of PRD with potassium humate at rates of 1.0% or 2.0%, along with copper at rates of 0.1% and 0.2%, outperformed the individual application of 100% PRD in terms of the measured parameters. Therefore, it can be concluded that the combined addition of potassium humate (as soil addition) and Cu (as foliar application) has a vital role in improving the quantitative and qualitative yield of the as well as raising the efficiency of phosphate fertilizers.

Metabolomics Reveals the Effects of Nitrogen/Phosphorus/Potassium (NPK) Fertilizer Levels on Cucumber Fruit Raised in Different Nutrient Soils.

Fertilizers are widely used to improve the quality of fruits and vegetables. However, the overuse of fertilizers has become an issue because it causes environmental problems and negatively affects productivity and fruit quality. In this study, we examined the effects of nitrogen, phosphorus, and potassium (NPK) fertilizer levels on the metabolism of cucumber fruit in low- and high-nutrient soils using mass-spectrometry-based metabolomics approaches. Cucumber metabolite content was notably different depending on the initial soil nutrient status. Most amino acids and phenylpropanoids were abundant in the cucumbers raised in low-nutrient soil, whereas organic acids, some amino acids (aspartate, glutamate, and ornithine), and carbohydrates were comparatively higher in fruits from high-nutrient soil. The fertilizer supply resulted in an alteration in the metabolite profile, while no change in fruit yield was observed in either low- or high-nutrient soils. Fertilizer treatment perturbed the metabolite contents in cucumbers from low-nutrient soil. In contrast, treatment with higher concentrations of fertilizer in high-nutrient soil increased phenylpropanoid content in the cucumbers, while most metabolites decreased. In conclusion, fertilization levels should be carefully determined, considering culture conditions such as the original soil status, to increase product yield and fruit quality and avoid environmental problems.

Interactive effects of long-term management of crop residue and phosphorus fertilization on wheat productivity and soil health in the rice–wheat

In the context of degradation of soil health, environmental pollution, and yield stagnation in the rice–wheat system in the Indo-Gangetic Plains of South Asia, an experiment was established in split plot design to assess the long-term effect of crop residue management on productivity and phosphorus requirement of wheat in rice–wheat system. The experiment comprised of six crop residue management practices as the main treatment factor with three levels (0, 30 and 60 kg P2O5 ha–1) of phosphorus fertilizer as sub-treatments. Significant improvement in soil aggregation, bulk density, and infiltration rate was observed under residue management (retention/incorporation) treatments compared to residue removal or residue burning. Soil organic carbon (SOC), available nutrient content (N, P, and K), microbial count, and enzyme activities were also significantly higher in conservation tillage and residue-treated plots than without residue/burning treatments. The residue derived from both crops when was either retained/incorporated improved the soil organic carbon (0.80%) and resulted in a significant increase in SOC (73.9%) in the topsoil layer as compared to the conventional practice. The mean effect studies revealed that crop residue management practices and phosphorus levels significantly influenced wheat yield attributes and productivity. The higher grain yield of wheat was recorded in two treatments, i.e. the basal application of 60 kg P2O5 ha–1 without residue incorporation and the other with half the P-fertilizer (30 kg P2O5 ha–1) with rice residue only. The grain yield of wheat where the rice and wheat residue were either retained/incorporated without phosphorus application was at par with 30 and 60 kg P2O5ha–1. Phosphorus levels also significantly affected wheat productivity and available P content in the soil. Therefore, results suggested that crop residue retention following the conservation tillage approach improved the yield of wheat cultivated in the rice–wheat cropping system.

Grain yield productivity, nutrient uptake and use efficiency and profitability of oat (Avena sativa L.) as influenced by variety and levels of nitrogen and phosphorus fertilizers in the central highlands of Ethiopia

Grain yield productivity, nutrient uptake and use efficiency and profitability of oat (Avena sativa L.) as influenced by variety and levels of nitrogen and phosphorus fertilizers in the central highlands of Ethiopia

Effects of phosphorus fertilizer and nano-magnesium spray on the vegetative and reproductive of Datura stramonium L.

In 2021, a field experiment was conducted at the College of Agriculture, University of Kerbala, Kerbala, Iraq, to study the effect of phosphate fertilization1 and nano-magnesium spraying on Datura plants' vegetative and reproductive traits. The experiment was conducted with three replications according to randomized complete block design (RCBD) with two factors. The first factor included four levels of Phosphorus fertilizer: 0, 25, 50, and 75 kg P h-1. The second factor included spraying of nano-magnesium in four concentrations: 0 (distilled water only) and 60, 120, and 180 mg Mg L-1. The results showed that phosphorous caused a significant increase in the studied traits, as the treatment 50 kg P ha-1 gave the highest mean plant height, number of branches, number of leaves, leaf area, leaf yield, seed yield, alkaloids percentage in leaves and seeds, number of fruits and number of seeds, which amounted to 4953 cm and 27.62 plant-1, 124.49 leaves Plant-1, 1989.12 cm2, 501.36 kg ha-1, 501.49 kg ha-1, 13.16%, 13.18%, 26.02 fruit plant-1 and 228.14 seed plant-1, respectively. The treatment 180 kg Mg h-1 gave the highest average plant height, number of branches, number of leaves, leaf area, yield of leaves, yield of seeds, percentage of alkaloids in leaves and seeds, number of fruits and number of seeds reaching 58.27 cm And 21.90 branches Plant-1, 122.94 leaves Plant-1, 1996.62 cm2, 886.59 kg h-1, 486.17 kg h-1, 14.86%, 15.10%, 26.56 fruits plant-1, 24.63 seeds plant-1. Adding phosphorous and spraying nano-magnesium improves the vegetative and reproductive traits of the Datura plant. Keywords. Fertilization; Nano–Nutrients; Medicinal Plant; Yield; alkaloids.

The Effect of Phosphorus Fertilization on Transcriptome Expression Profile during Lentil Pod and Seed Development

Seed coat hardness and water permeability, which are determined by the accumulation of tannins through the phenylpropanoid pathway in the seed, are important lentil quality characteristics. The impact of seeds’ developmental stage and phosphorus (P) fertilization levels on tannin accumulation is still under research. Through RNA sequencing, this study explored the effect of three P treatments (P0, 6 mg kg−1; P1, 15 mg kg−1; and P2, 21 mg kg−1) and three seed maturity stages (S1, immature 2 mm seed in a flat pod; S2, fully developed seed within the pod; and S3, mature seed at the beginning of the pod’s discoloration) on lentil gene expression. The key findings highlighted a significant influence of the seed maturity stage on phenylpropanoid genes, with S1 displaying the highest expression levels, and on phosphorus-related Gene Ontology (GO) terms that presented the highest number of downregulated genes in the S3 to S1 comparison. P exhibited a targeted effect on the flavanone 3-hydroxylase (F3H) and flavonol synthase (FLS) genes and specific gene clusters, as shown by the differential gene expression analysis. This study investigates the molecular mechanisms related to phosphorus fertilization and seed maturity stages that influence tannin accumulation, offering valuable information for the enhancement of lentil product quality through breeding programs.

Barley (Hordeum vulgare L.) photosynthetic and water use efficiency response to dairy‐amended biochar

AbstractBarley (Hordeum vulgare L.) was grown in a greenhouse under a combination of treatments that tested the effects of two types of biochar amended with fermentation solids and dairy lagoon effluent at high and low levels of phosphorus fertilization. We hypothesized that a modified biochar dosed with iron in combination with a dairy amendment would yield the greatest photosynthetic assimilation rates of CO2 and water use efficiency. Furthermore, we hypothesized that these rates may differ between photosynthate demand represented by plant developmental stages, specifically the tillering, stem elongation, and heading stages. We found that photosynthetic assimilation was greatest at the tillering stage. Plant water use efficiency increased with commercially available fertilizer. Plant biomass at harvest was greatest among the chemical fertilizer (high and low levels of phosphorus fertilization) and low‐level fertilization of dairy lagoon, fermentation solid, and an iron dosed biochar. The results suggest that while the dairy‐amended biochar can maintain plant growth, it is not necessarily achieved through improved photosynthesis or water use.

Straw Mulching Combined with Phosphorus Fertilizer Increases Fertile Florets of Wheat by Enhancing Leaf Photosynthesis and Assimilate Utilization

Lack of soil moisture and phosphorus deficiency limits wheat grain yield in dryland areas. However, the moisture-conserving effect of straw mulching combined with phosphor fertilization on fertile florets per spike (FFS) and grain yield remains unclear. During the 2020–2021 and 2021–2022 growing seasons, we investigated the combined effects of straw mulching (0 and 8000 kg ha−1) and phosphorus fertilization (0, 75, and 120 kg P2O5 ha−1) on spike development, assimilates’ availability, and the photosynthetic properties of flag leaves by conducting a field experiment. Compared with no straw mulch control, straw mulching increased fertile spike, grain number per spike (15.6%), and grain yield (22.6%), and grain number per spike was the most important contribution to increasing wheat grain yield (46%). An increase in grain number per spike is associated with FFS. Compared with no straw mulch control, straw mulching increased FFS by 19.5%, and it increased with increasing phosphorus fertilization levels. Moreover, straw mulching combined with phosphorus fertilization promoted the light compensation point (LCP), light saturation point (LSP), net photosynthetic rate (Pn), Chl b, and the maximal photochemical efficiency of photosystem II (Fv/Fm) of flag leaves to produce carbohydrates. Our study has shown that the primary factor for the divergence in FFS under straw mulching and phosphorus application was the efficiency of assimilate utilization in the spike, which ultimately led to increased grain number per spike and grain yield.

Effect of Elevated CO2 and Temperature on Phosphorus Harvest Index of Wheat (Triticum aestivum L.) at Various Levels of Phosphorus Fertilization

The possible alteration in plant growth, productivity and phosphorus (P) demand of wheat under rising atmospheric CO2 and temperature is expected to influence the partitioning of P to different plant parts of wheat. A phytotron experiment was conducted to study the effects of elevated atmospheric CO2 (650 µmol mol-1) and elevated temperature (ambient + 3 °C) on phosphorus harvest index of wheat at various levels (control, 100% and 200% of recommended P) of P fertilization. While there was a slight increase (5.6%) in P harvest index of wheat under elevated CO2, the reverse trend (12.7% decline) was observed under elevated temperature with no consistent effect at various levels of P fertilization. Interestingly, there was a moderate decline (7.7%) in P harvest index of wheat under combined elevation of CO2 and temperature as compared to their ambient combination. The results suggest that temperature could be the dominant factor as compared to the atmospheric CO2 in deciding the overall impact of projected increase in atmospheric CO2 and temperature on P harvest index of wheat. As there is logically possible relationship between P harvest index of wheat and various physical and nutritional qualities of wheat grains such as grain Zn and Fe content and their bio-availability, grain protein content, seedling vigour, etc., the decline in wheat’s P harvest index under projected levels of CO2 and temperature in our study underscores the need of undertaking elaborate experimentations to investigate the probable effects of climate change on quality parameters associated with P harvest index of wheat.

Rye Production under Acid Soils and Drought Conditions: An Alternative for the Sustainability of High Andean Livestock Farming in Peru

The rye (Secale cereale L.) crop shows a high potential to contribute to the sustainability of high Andean livestock because it supports the agroclimatic conditions and acid soils in the Peruvian Andes. The production of green forage, hay, and grain from the rye crop in acid soils was studied with the use of different levels of phosphorus and potassium fertilization in four local rye ecotypes (CBI-001, CSM-001, CJS-001, and CCE-001). The green forage yield (GFY) ranged from 32.35 to 53.62 t ha−1, dry matter from 6.05 to 8.56 t ha−1, and hay from 7.0 to 10.36 t ha−1; nutritional levels ranged from 9.02% to 13.56% protein and 6.50% to 7.75% ash levels, mainly with differences between ecotypes (p < 0.05). No differences existed between fertilization levels for the number of stems per plant, spikes per plant, and grains per ear (p > 0.05). Also, CBI-001 and CCE-001 were superior with 1868.4 and 1797.8 kg ha−1 of grain, respectively (p = 0.0072); the use of 60 kg ha of nitrogen, 120 kg ha−1 of P2O5, and 80 kg ha−1 of K2O gave higher grain and residue yields. The high nutritional value and yield of the rye ecotypes studied in acid soil conditions and without irrigation can be an alternative for livestock feeding and grain production in the rainy season in the Andes as a dual-purpose crop.

Sustainable production ofSaussurea costus under different levels of nitrogen, phosphorus and potassium fertilizers in cold desert region of Western Himalaya.

Saussurea costus, an important critically endangered medicinal herb native to the Himalayan region, is commonly used in various ailments, viz. asthma, ulcer, inflammation, and stomach problems. In the international market, the dry roots and essential oil of S. costus has become an important drug. The lack of appropriate fertilizer dose recommendations is one of the limiting factors for its ex-situ conservation and large-scale cultivation, as plant nutrition is vital in determining crop growth and productivity. The study aimed to understand the comparative impact of different levels of fertilizer nutrients on growth, dry root and essential oil yield, and essential oil profile of S. costus. A field experiment was conducted in Himachal Pradesh, India's cold desert region (Lahaul valley), during 2020-21. The experiment comprised three levels of nitrogen (60, 90, and 120 kg ha-1), three levels of phosphorus (20, 40, 60 kg ha-1), and two levels of potassium (20 and 40 kg ha-1) in a factorial randomized block design. The fertilizer application had an immense effect on growth attributes, root yield attributes, dry root yield, and essential oil yield over control. The treatment combination N120, P60, and K40 had the largest effect on the plant height, number of leaves per plant, leaf length and width, root length and diameter, dry matter per plant, dry root yield, and essential oil yield. However, the results were at par with the treatment comprising N90, P40, and K20. Dry root yield increased by 108.9%, and essential oil yield increased by 210.3% with fertilizer applications over unfertilized plots. The regression curve shows an increasing trend in dry root yield till N90, P40, and K20; after that, it nearly stabilized. The heat map showed that applying fertilizer significantly affected the chemical constituents of S. costus essential oil. Similarly, the plots fertilized with the highest level of NPK recorded the utmost value of available N, P, and K, as compared to unfertilized plots. The results emphasize that for sustainable cultivation of S. costus, the application of N90, P40, and K20 combinations is the most suitable one.

Quantitative and Qualitative Yield of Groundnut (Arachis Hypogaea L.) in Response to Intercropping Pattern and Integrated Application of Chemical and Biological Phosphorus Fertilizers

ABSTRACT Intercropping between cereals and legumes can increase resource use efficiency, agricultural productivity, and yield sustainability and reduce chemical inputs. For these reasons, this experiment was performed as factorial arrangement based on randomized complete block design with three replications in experimental station of Guilan Agricultural and Natural Resources Research and Education Center, Rasht, Iran in 2016 and 2017 cropping seasons. Experimental treatments included five levels of phosphorus fertilizer (PF) as triple super phosphate (TSP) including : 1- Zero (as control), 2–50 kg ha-1 TSP, 3–100 kg ha-1 TSP, 4–50 kg ha-1 TSP+200 g ha-1 Barvar2 phosphate bio-fertilizer (BPB) and 5–100 kg ha-1 TSP+200 g ha-1 BPB, and five IPs (IP) including: 1- maize (Zea mays L.) sole cropping, 2- groundnut sole cropping, 3- intercropped groundnut-maize with the ratio of 1:1, 2:1 and 1:2 rows, . Although, maximum grain yield of maize and groundnut was obtained in response to the application of 100 kg ha-1 TSP plus 200 g ha-1 BPB under sole cropping, the highest land equivalent ratio (LER) was observed under zero usage of TSP and intercropped maize-groundnut with the ratio of 1:1 rows. Crops in intercropping system have a complementary effect together. In this experiment, LER values greater than 1.00 indicated an intercrop advantage to sole crop. Results showed that IP increases the quantity and quality of products by increment in plant diversity, ecological balance, more utilization of resources, and reduction of damage by pests, diseases, and weeds in agricultural ecosystems.

Response of Sweet Sudan Grass to Graded Levels of Phosphorus and Potassium Fertilization

Background: Green fodder yield potential of the landmark multi-cut sweet sudan grass (SSG) variety SSG 59-3 was influenced due to the poor fertility status at many places in Haryana. The performance of sweet sudan grass under different P and K treatments was assessed by measuring fodder yield, agronomic efficiency, physiological efficiency and net income. Methods: A two-year (2018 and 2019) field study was conducted at Hisar, Haryana to assess the effect of four P levels (0, 6.6, 13.2 and 19.8 kg/ha P) and four K levels (0, 12.5, 25.0 and 37.5 kg/ha K) on growth, yield and quality of sweet sudan grass (SSG-59-3). The sixteen treatment combinations were replicated thrice in a factorial RBD. The sandy-loam soils of the experimental field was low in organic carbon (0.47%) and available nitrogen (141 kg/ha) and medium in available P (12.9 kg/ha) and K (225 kg/ha). The nutrients P and K were applied through di-ammonium phosphate and muriate of potash just before sowing. Result: The graded levels of P and K gave significantly better results up to 13.2 kg/ha P and 37.5 kg/ha K. The agronomic efficiency, physiological effecicency and apparent nutrient recovery (%) of nutrients were highest with lower levels of P and K (6.6 and 12.5 kg). The application of K had complementary effect on the uptake of P as appropriate ratios of N, P and K fertilizer improved absorption and utilization of nutrients.Therefore, farmers can apply 13.2 kg P/ha plus 37.5 kg K/ha along with recommend N for increased dry fodder yield and net income in sweet sudan grass.

Effect of spraying with potassium silicate and phosphorus fertilizer levels on growth, yield and fruit quality of eggplant plants under low temperatures stress.

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Role of Arbuscular Mycorrhizal Fungi and Phosphate Solubilizing Bacteria in Improving Yield, Yield Components, and Nutrients Uptake of Barley under Salinity Soil

Barley (Hordeum vulgare, L.) is the fourth most important cereal crop in the world. Salinity decreases the productivity of plants grown under salinity conditions. It leads to deficiency and limited absorption of water and nutrients, ionic stress, oxidative stress, and osmotic imbalance. In saline soil, a field experiment was conducted to verify the effects of nine combinations among three levels of bio-fertilizers, i.e., control (without), arbuscular mycorrhizal fungi (AMF), and phosphate solubilizing bacteria (PSB), as well as three levels of phosphorus fertilizer recommended dose (RDP) on barley yield, its components and nutrients uptake, to evaluate the useful influences of these combinations to improve P management under salinity stress related to yield and its components as well as N, P, and K uptake in barley. Findings revealed that the combination AMF + 100% RDP improved plant height, length of spike, spikes weight, number of spikes plant−1, weight of 1000-grain, straw yield, grain yield, uptake of nitrogen (N), phosphorus (P), potassium (K) in grain and uptake of nitrogen (N), phosphorus (P), potassium (K) in straw by 19.76, 33.21, 40.08, 33.76, 14.82, 24.95, 47.52, 104.54, 213.47, 168.24, 124.30, 183.59, and 160.84% in the first season, respectively. Meanwhile, the increase was 19.86, 29.73, 40.47, 39.94, 14.92, 24.95, 47.94, 104.73, 213.33, 168.64, 124.47, 183.86, and 161.09% in the second season, respectively. AMF showed greater efficiency and effectiveness compared to PSB in improving yield and its components for all studied traits. The results of principle component analysis indicated that all combinations except AMF + zero% RDP, PSB + zero% RDP, control + zero% RDP, and control + 66% RDP showed high scores on positive PC1, where all studied traits were high. Therefore, it is recommended to inoculate the soil with AMF or PSB with the addition of phosphate fertilizer at the recommended dose under salinity conditions, i.e., AMF + 100% RDP (T1) or AMF + 66% RDP (T2) or PSB + 100% RDP (T4). The use of bio-fertilizers has increased plant tolerance to salt stress, and this was evident from the increase in different traits with the use of treatments that include bio-fertilizers.

Impact of nitrogen and phosphorus fertilizers on Cry1Ac protein contents in transgenic cotton.

Application of different fertilizers to check the efficiency of expression of Bt (Bacillus thuringiensis) gene in one of the leading commercialized crops (cotton) against Lepidopteran species is of great concern. The expression of Cry protein level can be controlled by the improvement of nutrients levels. Therefore, the myth of response of Cry toxin to different combinations of NP fertilizers was explored in three Bt cotton cultivars. Combinations include three levels of nitrogen and three levels of phosphorus fertilizers. Immunostrips and Cry gene(s) specific primer based PCR (Polymerase Chain Reaction) analysis were used for the presence of Bt gene that unveiled the presence of Cry1Ac gene only. Further, the ELISA (enzyme-linked immunosorbent assay) kit was used to quantify the expression of Cry1Ac protein. Under various NP fertilizers rates, the level of toxin protein exhibited highly significant differences. The highest toxin level mean was found to be 2.3740 and 2.1732 µg/g under the treatment of N150P75 kg ha-1 combination while the lowest toxin level mean was found to be 0.9158 and 0.7641 µg/g at the N50P25 kg ha-1 level at 80 and 120 DAS (Days After Sowing), respectively. It was concluded from the research that the usage of NP fertilizers has a positive relation with the expression of Cry1Ac toxin in Bt cotton. We recommend using the N150P50 kg ha-1 level as the most economical and practicable fertilizer instead of the standard dose N100P50 kg ha-1 to get the desired level of Cry1Ac level for long lasting plant resistance (<1.5). The revised dose of fertilizer may help farmers to avoid the cross-resistance development in contradiction of insect pests.

USING PHOSPHATE SOLUBILIZING BACTERIA TO ENHANCE PRODUCTIVITY OF TWO LENTIL VARIETIES

Two field experiments were conductedunder surface irrigation system during two successive winter growing seasons 2017/2018 and 2018/2019in private farm atEl Qantra-West, Ismailia Governorate,Egyptto evaluate the effect of the combinations of three levels of phosphorus fertilizer (0, 15.5 and 31.0 kg P