Levels Of Peroxidation Products Research Articles

Improvement of high-density lipoprotein atheroprotective properties in patients with systemic lupus erythematosus after belimumab treatment.

Chronic inflammatory diseases, like Systemic Lupus Erythematosus (SLE), carry an increased risk for atherosclerosis and cardiovascular events, accompanied by impairment of atheroprotective properties of high-density lipoprotein (HDL). In SLE, serum BAFF (B cell-activating factor), a cytokine implicated in disease progression, has been correlated with subclinical atherosclerosis. We investigated the impact of treatment with belimumab -an anti-BAFF monoclonal antibody- on HDL atheroprotective properties and composition in SLE patients. Serum samples were collected from 35 SLE patients with active disease despite conventional therapy, before and after 6-month add-on treatment with belimumab, and 26 matched healthy individuals. We measured cholesterol efflux and antioxidant capacities, paraoxonase-1 activity, serum amyloid A1, myeloperoxidase and lipid peroxidation product levels of HDL. LC-MS/MS was performed to analyze the HDL lipidome. Following treatment with belimumab, cholesterol efflux and antioxidant capacities of HDL were significantly increased in SLE patients and restored to levels of controls. HDL-associated paraoxonase-1 activity was also increased, whereas lipid peroxidation products were decreased following treatment. HDL cholesterol efflux and antioxidant capacities correlated negatively with the disease activity. Changes were noted in the HDL lipidome of SLE patients following belimumab treatment, as well as between SLE patients and healthy individuals, and specific changes in lipid species correlated with functional parameters of HDL. HDL of SLE patients with active disease displays impaired atheroprotective properties accompanied by distinct lipidomic signature compared with controls. Belimumab treatment may improve the HDL atheroprotective properties and modify the HDL lipidomic signature in SLE patients, thus potentially mitigating atherosclerosis development.

Thermal tolerance of a freshwater amphipod Gammarus lacustris can be enhanced by acclimation to higher mineralization

Temperature and mineralization are among the most important environmental factors affecting all processes of aquatic ecosystems, including geographical distribution of water animals. Previously we showed that a brackish water population of Gammarus lacustris, a widespread amphipod, demonstrates substantially higher thermotolerance than a freshwater population. A possible reason for this difference is the fact that brackish water conditions are closer to internal media mineralization. Here we aimed to test this hypothesis and relate the observed effects in animal survival under the heat shock to the status of cellular defence systems. We acclimated four groups of amphipods from the same freshwater population to 0.5 ‰ and 15 ‰ at the temperatures of 6°С or 15°С. Acclimation at 6°С, but not at 15°С, to 15 ‰ significantly increased resistance of the amphipods to heat shock at 30°C. At 6°С activities of antioxidant enzymes and levels of the lipid peroxidation products in G. lacustris did not react to the increase in mineralization and the heat shock, while the level of HSP70 elevated two-fold in amphipods acclimated to mineralization of 15 ‰ compared to animals acclimated to 0.5 ‰. Thus, the observed increase in thermotolerance could be explained by the higher initial level of HSP70 and potentially other heat shock proteins caused by a less energy-demanding, more isotonic level.

EFFECT OF ACTG(4-7)-PRO-GLY-PRO AND ACTG(6-9)-PRO-GLY-PRO ON THE INTENSITY OF REDOX PROCESSES AND BEHAVIOURAL STATUS OF RATS UNDER THYROID HYPERFUNCTION CONDITIONS

The aim of the work is to study the effect of melanocortins on the intensity of redox processes in the prefrontal region of the brain and the behavioral status of rats against the background of thyroid hyperfunction. Material and methods. In the experiment, the test animals were divided into the following groups (n = 10): intact rats (control); animals treated with sodium salt pentahydrate L-thyroxine (hyperthyroidism); rats receiving ACTH (4-7)-Pro-Gly-Pro and individuals receiving ACTH (6-9)-Pro-Gly-Pro, re-spectively, intraperitoneally daily for 21 days, starting from day 22 of administration of sodium salt pentahydrate L-thyroxine. Hyperthyroidism was simulated in animals by intragastric administration of L-thyroxine sodium salt pentahydrate for 42 days. The intensity of lipoperoxidation processes was studied by the content of diene and triene conjugates in the prefrontal zone tissue, the initial level of TBА-reactive products, and the rates of spon-taneous and ascorbic peroxidation of lipids. The level of protein peroxidation products was determined by the reaction of oxidized amino acid residues of proteins with 2,4-dinitrophenyl hydrazone. Evaluation of the enzymatic unit of the antioxidant system of the prefrontal region was carried out by de-termining the activity of superoxide dismutase and catalase. The behavioral status of laboratory rodents was studied using the «Hole board» test. Results. The use of ACTH(4-7)-Pro-Gly-Pro and ACTH(6-9)-Pro-Gly-Pro compounds under experimental hyperthyroidism led to a decrease in the intensity of the processes of peroxidation of lipids, proteins and the restoration of levels of activity of antioxidant enzymes – superoxide dismutase and catalase in the tissue of the prefrontal region of the brain of animals, as well as correction behavioral indicators in the «Hole board» test. Conclusion. The obtained experimental data indicate that ACTH(4-7)-Pro-Gly-Pro and ACTH(6-9)-Pro-Gly-Pro show antiperoxy and antioxidant activity against lipoperoxidation parameters and oxidative modification of proteins and enzymatic protection systems in the prefrontal brain zone tissue, as well as exert psychocorregating action on behavioral responses of white rats against the background of thyroid hyperfunction.

Combined effects of norfloxacin and polystyrene nanoparticles on the oxidative stress and gut health of the juvenile horseshoe crab Tachypleus tridentatus

Pollution with anthropogenic contaminants including antibiotics and nanoplastics leads to gradual deterioration of the marine environment, which threatens endangered species such as the horseshoe crab Tachypleus tridentatus. We assessed the potential toxic mechanisms of an antibiotic (norfloxacin, 0, 0.5, 5 μg/L) and polystyrene nanoparticles (104 particles/L) in T. tridentatus using biomarkers of tissue redox status, molting, and gut microbiota. Exposure to single and combined pollutants led to disturbance of redox balance during short-term (7 days) exposure indicated by elevated level of a lipid peroxidation product, malondialdehyde (MDA). After prolonged (14–21 days) exposure, compensatory upregulation of antioxidants (catalase and glutathione but not superoxide dismutase) was observed, and MDA levels returned to the baseline in most experimental exposures. Transcript levels of molting-related genes (ecdysone receptor, retinoic acid X alpha receptor and calmodulin A) and a molecular chaperone (cognate heat shock protein 70) showed weak evidence of response to polystyrene nanoparticles and norfloxacin. The gut microbiota T. tridentatus was altered by exposures to norfloxacin and polystyrene nanoparticles shown by elevated relative abundance of Bacteroidetes. At the functional level, evidence of suppression by norfloxacin and polystyrene nanoparticles was found in multiple intestinal microbiome pathways related to the genetic information processing, metabolism, organismal systems, and environmental information processing. Future studies are needed to assess the physiological and health consequences of microbiome dysbiosis caused by norfloxacin and polystyrene nanoparticles and assist the environmental risk assessment of these pollutants in the wild populations of the horseshoe crabs.

Piceatannol protects against myocardial ischemia/reperfusion injury by inhibiting ferroptosis via Nrf-2 signaling-mediated iron metabolism

Myocardial tissue ischemia damages myocardial cells. Although reperfusion is an effective technique to rescue myocardial cell damage, it may also exacerbate myocardial cell damage. Ferroptosis, an iron-dependent cell death, occurs following myocardial ischemia-reperfusion (I/R). Piceatannol (PCT) is a natural stilbene compound with excellent antioxidant properties that protect against I/R injury and exerts protective effects against ferroptosis-induced cardiomyocytes following I/R injury; however, the exact mechanism remains to be elucidated. PurposeThis study aims to investigate the protective effect and mechanism of PCT on myocardial ischemia-reperfusion injury. MethodsAn ischemia-reperfusion model was established via ligation of the left anterior descending branch of mice's hearts and hypoxia-reoxygenation (H/R) of cardiomyocytes. ResultsDuring ischemia-reperfusion, Nuclear factor E2-related factor 2 (Nrf-2) expression was downregulated, the left ventricular function was impaired, intracellular iron and lipid peroxidation product levels were elevated, and cardiomyocytes underwent ferroptosis. Furthermore, ferroptosis was enhanced following treatment with an Nrf-2 inhibitor. After PCT treatment, Nrf-2 expression significantly increased, intracellular ferrous ions and lipid peroxidation products significantly reduced, Ferroportin1 (FPN1) expression increased, and transferrin receptor-1 (TfR-1) expression was inhibited. ConclusionsPCT regulates iron metabolism through Nrf-2 to protect against myocardial cell ferroptosis induced by myocardial I/R injury.

The effect of a medicinal plant preparation on the frequency of episodes of exacerbation of recurrent cystitis and metabolic parameters in patients with type 2 diabetes mellitus taking glyphlosins

The problem of recurrent urinary tract infections (UTI) in patients with type 2 diabetes mellitus (DM 2) is relevant, especially when there is a combination of predisposing factors, such as female gender, history of UTI episodes, and therapy with sodium glucose cotransporter type 2 (SGLT-2) inhibitors, and the choice of effective and safe means could cause some difficulties, including ina terms of the burden of antibiotic resistance. To evaluate the effectiveness and safety of the phytoproduct Canephron® N for the prevention of exacerbations of recurrent cystitis and the effect on metabolic parameters in patients with type 2 diabetes taking SGLT-2 inhibitors. Prospective, randomized, open, parallel group study in 60 women. The main group took the drug Canephron® N for 3 months. The main parameters for evaluating were the frequency of recurrence of cystitis, level of albuminuria and LDL-cholesterol peroxidation product - malondialdehyde. Within 3 months of taking Canephron® N, exacerbations of chronic cystitis were diagnosed 2 times less often, a decrease in albuminuria was found in the form of an increase in the proportion of patients with an optimal level of albuminuria by 20%, a 50% reduction in the frequency of the initial increase in albuminuria, and the absence of moderate albuminuria in all patients at the end of course of therapy. A decrease in the level of MDA by 1.4 times was noted (p=0.019). Thus, the herbal drug Canephron® N can be used for accompanying therapy and prophylactic treatment in patients with recurrent cystitis on the background of DM 2, taking SGLT-2 inhibitors. The course of therapy should last at least 3 months.

PAK2/beta-catenin/c-Myc/PKM2 signal transduction suppresses ovarian granulosa cell apoptosis in polycystic ovary syndrome

Polycystic ovary syndrome (PCOS) exhibits the highest morbidity among endocrine diseases in women ranging from age 18 to 44. However, its pathogenesis remains unclear. The imbalance between systemic and ovarian oxidative stress (OS) is a key characteristic of PCOS, and accumulating evidence indicates that the antioxidative protein nuclear factor erythroid-2-related factor 2 (Nrf2) is implicated in cell apoptosis and inflammation caused by OS. The activated kinase 2 (PAK2)/-catenin/c-Myc/pyruvate kinase M2 (PKM2) axis is a newly identified signaling pathway that may regulate Nrf2 expression and thereby influence OS. In this study, we sought to identify PAK2 expression and function in PCOS cells. PAK2 and downstream PKM2 expression in KGN cells and tissues were detected by microarray and qPCR. Cell viability was determined using CCK-8 and colony formation assays (CFAs). Apoptosis was examined by flow cytometry. qPCR and ELISA were used to examine cell inflammation. Oxidant and OS-related enzymes were examined by ELISA. We found that PAK2 and PKM2 expression levels were reduced in KGN cells and PCOS ovarian cortex tissues. PAK2 overexpression activated β-catenin/c-Myc/PKM2 while PAK2 silencing deactivated it. PAK2 overexpression was reduced, whereas PAK2 silencing promoted, KGN cell proliferation and colony formation. Cell apoptosis and inflammation were also induced by PAK2 overexpression but were alleviated by its silencing. Furthermore, increased peroxidation product levels decreased antioxidative protein activities, and deactivated antioxidative Nrf2/HO-1 pathway were detected in PAK2-overexpressing KGN cells, whereas these effects were counteracted in PAK2 silenced cells. Our data suggest that PAK2 and its associated β-catenin/c-Myc/PKM2 inhibited cell viability and induced apoptosis and inflammation by triggering OS by deactivating the Nrf2/HO-1 pathway, suggesting the potential of PAK2 as a therapeutic PCOS treatment target.

Bioactivity of Falkenbergia rufolanosa Methanolic Extract: Assessment of Its Effect on Methyl-Thiophanate Induced Bone and Blood Disorders.

This study aimed to evaluate the potentiality of a mineral and antioxidant-rich methanolic extract of the red marine alga Falkenbergia rufolanosa (FRE) against methyl-thiophanate (MT)-induced toxicity in adult rats. The animals were allocated into four groups: controls, MT (300 mg/kg), MT + FRE, and FRE-treated group for 7 days. Our results demonstrated severe mineral perturbations due to MT treatment, especially in calcium and phosphorus levels in plasma, urine, and bone. Similarly, the hematological analysis revealed increased red blood cells, platelets, and white blood cells associated with striking genotoxicity. Interestingly, a significant rise in lipid peroxidation and advanced oxidation protein products level in erythrocytes and bone were noted. Meanwhile, a depletion of the antioxidant status in both tissues occurred. These biochemical alterations were in harmony with DNA degradation and histological variation in bone and blood. In the other trend, data showed that treatment with alga improved MT-induced hematotoxicity, genotoxicity, and oxidative stress in the blood and bone. Osteo-mineral metabolism and bone histo-architecture were also noted. In conclusion, these findings demonstrated that the red alga Falkenbergia rufolanosa is a potent source of antioxidant and antibacterial agents, as revealed by the in vitro analysis.

Homeostatic effect of silicon waters

Introduction. There are considered drinking and mineral waters in terms of homeostatic action (hormesis) of water-soluble silicon and the trends of its biological activity and possible negative effects on the human body.
 Materials and methods. In studied waters the concentration of the systemic homeostatic regulator – peroxide anion-radical was measured by the kinetic chemiluminescent method. The water biological activity was assessed by the extrasubstrate synthesis of adenosine triphosphate by the ATP synthase of microorganisms E.Coli K12 TG1(pF1) with built-in genes for the complete CDABE lux operon of the bacteria V. fischeri 6E luminescent system. The parametrization of the water structural-energy state was carried out according to the cryophysical capillary technique. The study object were low-mineralized drinking water packed in 9-liter polymer containers with an initial silicon content of 12 mg/dm3.
 Results. The hormetic effect of silicon waters due to self-induction of peroxide anion radicals in water manifests itself in the form of mitochondrial activity and enzyme complexes stimulation, accompanied by activation of the central nervous system, maintenance of nonspecific immunity, and the reproductive system functioning. The degree of silicon water beneficial effect due to the electron-donor and regulatory action of peroxide anion-radicals associates, depends on activation processes during the production and drinking water storage.
 Limitations. Recommendations for maintaining the biological activity of silica waters do not go beyond the limits established by existing regulatory documents on the safety of drinking waters.
 Conclusion. The levels of radical anion peroxide production (2.6–5.2 μg/dm3), obtained in the course of this study, correspond to the maximum changes in the biological activity of water activation in the range of 1.4–3.0 (relative to the control – distilled water) in the mode of implementing the conditions of non-local activation. With long-term storage in a polymer container, the ability to activate is lost – such water becomes biologically inert.

Correlation analysis between inflammatory biomarkers and significant clinical phenotypes of chronic obstructive pulmonary disease

Introduction: Chronic obstructive pulmonary disease (COPD) is the leading cause of mortality. Using the evidence obtained about various clinical phenotypes of patients with the same disease allowed us to expand our understanding of the treatment of COPD. Nowadays the only option for solving the problem will be the definition of the clinical phenotype of COPD, and the receipt of expanded data on its correlation with respiratory and other significant biomarkers. Materials and methods: We analyzed the correlations between FKN, CRP and TBA-active lipid peroxidation products in 373 patients with various COPD phenotypes and 60 healthy volunteers. Enzyme immunoassay was used to study the levels of inflammatory biomarkers. Results: We have identified a statistically significant increase in the levels of inflammatory biomarkers in patients with COPD compared with the control. The FKN level in the group of patients with COPD was 1.3 ng/ml, which was higher (p<0.001) than in the control (FKN level of 0.3 ng/ml, p<0.001). The CRP level in patients with COPD was 27.8 mg/L, whereas in control the CRP level was 1.2 mg/L (p<0.001). The TBA-active lipid peroxidation products level in patients with COPD was 14.5 mmol/L, which was higher when compared to the control (p<0.001). Discussion: The correlation analysis revealed very strong relationships between the levels of all the biomarkers studied. The highest values of the Kendall rank correlation coefficient (τ) were determined between the levels of all the inflammatory biomarkers in subgroups of patients with chronic bronchitis and mixed COPD phenotypes. Conclusion: Detection of the COPD phenotype will help actively monitor the therapy of COPD exacerbations. Graphical Abstract

Influence of Methyl Jasmonate and Salicylic Acid as Components of the Cultivation Medium for Rhizobium on Formation of Different-Efficiency Symbiotic Systems Glycine max — Bradyrhizobium japonicum

Phytohormones are important in the main pathways of transduction of symbiotic signals between macro- and microsymbionts, and understanding of their participation in integration with other metabolic pathways, including prooxidant-antioxidant systems, is crucial in the formation of different-efficiency symbiotic systems. Aim. To investigate the effect of salicylic acid (SA, 50 μM) and methyl jasmonate (MJ, 0.75 μM) as components of the cultivation media of different in the activity and virulence rhizobia 604k and B1-20 on the peculiarities of the formation of symbiotic systems in terms of intensity and activity of catalase, as well as on the course of nodulation and nitrogen fixation. Methods. Microbiological (cultivation of nitrogen-fixing microorganisms, seed inoculation), physiological (vegetation experiment), biochemical (spectrophotometry, gas chromatography), and statistical. Results. It was found that the use of SA (50 μM) as an additional component of the culture medium of active rhizobia Tn5-mutant B1-20 for seed inoculation induces increased levels of peroxide production and catalase activity in soybean roots in the early stages of symbiosis, which contributes to the effectiveness of its symbiotic apparatus. Addition of MJ (0.75 μM) to the culture medium of rhizobia B1-20 does not affect changes in peroxide content and catalase activity in the roots, however, stimulates the processes of nodulation and reduces nitrogen fixation. It has been shown that modification of the cultivation medium of inactive rhizobia of the highly virulent strain 604k using SA (50 μM) or MJ (0.75 μM) does not change the peroxide content and leads to an increase in catalase activity in soybean roots during the formation of an ineffective symbiotic system with activation of nodulation processes. Conclusions. When using SA (50 μM) or MJ (0.75 μM) as components of the culture medium for rhizobia of different activity and virulence (604k and B1-20), differences were recorded in the levels of functioning of pro-antioxidant systems, in particular, in the production of peroxides and active catalase complexes, at the early stages of the formation of symbiotic systems of Glycine max — Bradyrhizobium japonicum, which affects the intensity of the processes of nodulation and nitrogen fixation.

Physiological response of coriander plant - grown in Duba, Saudi Arabia - to NaCl stress

A pot experiment was conducted (in Duba, Saudi Arabia) to evaluate the impact of NaCl salinity (100, 200, 300 and 400 mM) on some physiological traits of coriander plants grown for 30 days in the greenhouse. Irrigating plants with saline water decreased N, P, K and Mg contents, but increased Na content in leaves. Raising salinity decreased chlorophyll (Chl) concentrations of leaves as well as Chl a to b ratio. Salinity induced a marked increase in total-soluble sugars, total-soluble proteins and proline concentrations of leaves. NaCl stress caused an increase in H2O2 and malondialdehyde (a peroxidation product) levels coupled with significant enhancement in the activities of antioxidant enzymes (superoxide dismutase, peroxidase and catalase), reflecting the peroxidation of membrane lipids which led to the loss of membrane selective permeability. Shoot growth (height, fresh and dry masses) and leaf number were inhibited by increasing salinity, while both root growth and root to shoot ratio were increased. Therefore, the test variety of coriander seemed to be moderate salt-tolerant and is not promising to be cultivated in salinized soils with salt levels more than 200 mM, although it has been grown until 300 mM and died at 400 mM NaCl.

Diet and SIRT1 Genotype Interact to Modulate Aging-Related Processes in Patients with Coronary Heart Disease: From the CORDIOPREV Study.

We investigated whether long-term consumption of two healthy diets (low-fat (LF) or Mediterranean (Med)) interacts with SIRT1 genotypes to modulate aging-related processes such as leucocyte telomere length (LTL), oxidative stress (OxS) and inflammation in patients with coronary heart disease (CHD). LTL, inflammation, OxS markers (at baseline and after 4 years of follow-up) and SIRT1-Single Nucleotide Polymorphisms (SNPs) (rs7069102 and rs1885472) were determined in patients from the CORDIOPREV study. We analyzed the genotype-marker interactions and the effect of diet on these interactions. Regardless of the diet, we observed LTL maintenance in GG-carriers for the rs7069102, in contrast to carriers of the minor C allele, where it decreased after follow-up (p = 0.001). The GG-carriers showed an increase in reduced/oxidized glutathione (GSH/GSSG) ratio (p = 0.003), lower lipid peroxidation products (LPO) levels (p < 0.001) and a greater decrease in tumor necrosis factor-alpha (TNF-α) levels (p < 0.001) after follow-up. After the LF diet intervention, the GG-carriers showed stabilization in LTL which was significant compared to the C allele subjects (p = 0.037), although the protective effects found for inflammation and OxS markers remained significant after follow-up with the two diets. Patients who are homozygous for the SIRT1-SNP rs7069102 (the most common genotype) may benefit from healthy diets, as suggested by improvements in OxS and inflammation in patients with CHD, which may indicate the slowing-down of the aging process and its related diseases.

Parameters of the Blood Oxidant/Antioxidant System in Elderly Patients with Acute Poisoning

The aim of the study was to assess the oxidant/antioxidant status in elderly patients in the early period of acute poisoning by psychotropic drugs or corrosive substances.Material and methods. An open prospective observational study with retrospective control was conducted in 80 patients (age ≥ 60 years) with acute poisoning, of which 49 patients aged 72.1±9.55 years had psychotropic drug poisoning (PDP) and 31 subjects aged 73.0±10.3 years had corrosive substance poisoning (CSP). Patients with mild poisoning were excluded from the study. The control group consisted of 39 volunteers aged 68.3±6.3 years. Total antioxidant status (TAS), blood levels of malondialdehyde (MDA), stable nitric oxide metabolites (nitrite/nitrate, NOx), and oxidative stress index (MDA/TAS) were measured on days 1, 3 and 5 after hospital admission.Results. When analyzing the changes in the parameters of the oxidant/antioxidant system, we observed lower values of the studied parameters in patients with both PDP and CSP compared to the control group. In patients with PDP, several parameters were reduced: MDA by 1.2 times on days 1 and 3 (P=0.002; P=0.008, respectively), NOx by 1.7 times (P&lt;0.001) at all stages of the study, MDA/TAS by 2.4–2.9 times (P&lt;0.001). In patients with CSP, MDA level decreased by 1.1–1.2 times at all study timepoints (P=0.003; P=0.010; P=0.046, respectively), NOx dropped 1.4–1.6-fold (P=0.012; P=0.004; P=0.023, respectively), and MDA/TAS decreased by 2.3–2.4 times (P&lt;0.001). While comparing patients with favorable and fatal outcome, we found that in survived patients an increase of MDA/TAS along with growing NOx level was seen by day 5 with no significant changes of MDA and TAS, while in non-survivors MDA/TAS dropped continuously due to progressive fall of NOx level, reaching values 2.8–2.9 times (P&lt;0.001) lower than those of the controls.Conclusion. In elderly patients with acute poisonings due to psychotropic drugs or corrosive substances, an inadequate response of the oxidant/antioxidant system occurs manifesting as a reduced blood level of peroxidation products with simultaneous normal or slightly decreased concentration of antioxidant protection system components. Thus, the oxidative stress develops, which contributes to the death of the patients.

Изменение антиоксидантно/прооксидантного равновесия у пациентов с приобретенными укорочениями костей голени на этапах удлинения по Илизарову

Introduction Optimization of the conditions of distraction osteogenesis using the method of G.A. Ilizarov remains an important challenge to ensure a reduction in the number of complications and an increase in the effectiveness of this technology. The aim of the study was to study the changes in the antioxidant/prooxidant balance in patients with acquired shortening of the lower leg bones at the stages of the Ilizarov lengthening procedure. Methods The antioxidant/prooxidant status was studied in 12 patients with shortening of one of the lower legs resulting from hematogenous osteomyelitis (group 1) and in 13 patients with post-traumatic shortening of one of the lower legs (group 2). The level of peroxidation products, the activity of superoxide dismutase and vitamins E and A in the blood of these patients were assessed in the dynamics of monofocal bone lengthening using the Ilizarov method. Results It was found that in patients with acquired shortening of the shin bones, regardless of the shortening etiology, during surgical lengthening, an increase in the level of peroxidation products in the blood was observed with a decrease in the level of vitamin E and an increase in superoxide dismutase activity. Discussion The data obtained, together with the current findings from fundamental research, allow us to note that the use of antioxidants (AOs) in patients undergoing segment length correction using the Ilizarov technology is possible from two positions. 1. The positive effect of AOs in terms of stimulation of distraction osteogenesis has been proven. 2. AOs have a systemic effect in the regulation of the recovery processes in the paraosseous tissues of the limb segment under lengthening. Conclusion Peroxidation is activated in the course of surgical lengthening of the lower leg bones in patients with acquired shortenings, regardless of the shortening etiology. The use of antioxidants in such patients can specifically inhibit peroxidation, thereby reducing the risk of provoking bone formation disorders.

Local and Systemic Oxidative Stress Biomarkers for Male Infertility: The ORION Study.

Infertility problems occur in around 10% of all couples worldwide, with male-factor infertility as the sole contributor in 20–30% of these cases. Oxidative stress (OS) is suggested to be associated with the pathophysiology of male infertility. In spermatozoa, OS can lead to damage to the cell membrane, resulting in disruption of DNA integrity and a decrease in motility. Established biomarkers for OS include free thiols and malondialdehyde (MDA), both representing different components of the reactive species interactome (RSI). This exploratory study aimed to investigate seminal plasma-free thiol and MDA levels in relation to semen parameters as defined by the World Health Organization (WHO) to determine if these markers are adequate to define local OS status. Furthermore, this study investigated if there is a relation between systemic and local OS status by comparing seminal concentrations of free thiol (R-SH, sulfhydryl groups, representing the extracellular redox status) and MDA (lipid peroxidation product) levels to those measured in serum. Free thiol and MDA measurements in both serum and semen plasma were performed in 50 males (18–55 y) of couples seeking fertility treatment. A significant positive correlation was found between seminal plasma-free thiol levels and sperm concentration and progressive motility (r = 0.383, p = 0.008 and r = 0.333, p = 0.022, respectively). In addition, a significant positive correlation was found between MDA levels in seminal plasma and sperm concentration (r = 0.314, p = 0.031). This study supports that seminal plasma-free thiols may be promising as local OS biomarkers. No associations were observed between local and systemic OS biomarker concentrations.

The Effect of Lithium Salt with Ascorbic Acid on the Antioxidant Status and Productivity of Gestating Sows.

Simple SummaryThis research is aimed at studying the effect of lithium ascorbate on increasing the antioxidant status of gestational sows and reducing the level of lipid peroxidation products. The studies were conducted on gestational sows of the Irish Landrace breed. Thirty days after successful insemination, the sows of the experimental groups, E10, E5 and E2, began to receive lithium ascorbate powder together with compound feed in doses of 10, 5, and 2 mg/kg of live weight, respectively. Weighing and biochemical examination were carried out before the introduction of the substance, as well as on the 60th and 110th days of gestation. In the blood plasma of sows, the following were determined: malondialdehyde, reduced glutathione, oxidized glutathione, SH/SS ratio, activity of superoxide dismutase and glutathione peroxidase. The use of lithium ascorbate b caused a significant increase in the level of SH (reduced glutathione) by 21% (p < 0.05), a decrease in the level of SS (oxidized glutathione) by 17% (p < 0.05) and a decrease in the level of malondialdehyde by 60% (p < 0.05). The results obtained cause activation of the antioxidant defense system, reduce the risk of oxidative stress, and have a positive effect on the gestation process.This research is aimed at the influence of different doses of lithium ascorbate on pigs’ diet estimation, at farrowing sows’ antioxidant status increase, and at lipid peroxidation product level decrease. The research was conducted in farrowing sows of the Irish landrace breed during the second farrow. Three groups of animals were formed, with ten livestock units in each. Thirty days after successful insemination, the sows of the E10, E5 and E2 experimental groups started receiving lithium ascorbate powder together with feed stuff in dosages of 10, 5 and 2 mg/kg of body weight, respectively. Their weighing and biochemical examinations were performed before the substance introduction as well as on the 60th and 110th days of pregnancy. The following were detected in sows’ blood plasma: malondialdehyde, reduced glutathione, oxidized glutathione, SH/SS ratio, superoxide dismutase and glutathione peroxidase activity. Lithium ascorbate usage during sows’ breeding cycle caused a significant increase in SH (reduced glutathione) level by 21% (p < 0.05), SS (oxidized glutathione) level decrease by 17% (p < 0.05), and malondialdehyde level decrease by 60% (p < 0.05). These data outline antioxidant defense system activization, reducing the risk of oxidative stress under the influence of feeding with lithium ascorbate. Lithium ascorbate in dosages of 10 mg/kg per body weight given together with feed stuff shows prominent adaptogene and stress protective features in the most effective way. The research conducted regarding lithium ascorbate usage for farrowing sows can reduce the negative consequences of oxidative stress, increase sows’ health preservation level, and contribute to fertility boost.

Evaluation of antioxidative enzymes levels and lipid peroxidation products levels in diabetic and non diabetic senile cataract patients.

The current study aimed to investigate the Superoxide dismutase, Glutathione Peroxidase activity, Advanced oxidation Protein products levels, Malondialdehyde levels, Baseline Conjugated Diene concentration, and 8-Isoprostaglandin F2α (8-IPG-F2α) quantification in diabetic and non-diabetic senile cataract patients to find out the pathomechanism of early onset of cataract in diabetic patients. This case-control study was performed on 184 subjects undergoing cataract surgery. For 8-IPG-F2α quantification, 35 diabetics and non-diabetic lenses and the entire study included 22 patients with diabetic senile cataracts and non-diabetic senile cataracts of aqueous humor age ≥ 40years. Ninety-two patients with diabetic senile cataract and non-diabetic senile cataract lenses aged ≥ 40years were incorporated for all other studies. The student's t-test was used for statistical analysis. From the study population, the mean age was 63.82 ± 0.6, and 75% of them were female. Higher female prevalence in both groups was observed. The results revealed that the superoxide dismutase and glutathione peroxidase activity were significantly reduced in diabetic patients compared to non-diabetic patients (p < 0.001) in both lenses and aqueous humor. Malondialdehyde, conjugated diene, and 8-IPG-F2α levels significantly increased in diabetic patients when compared to non-diabetic (p < 0.01), and advanced oxidation protein products levels also significantly increased in diabetic patients when compared to non-diabetics in both lenses and aqueous humor (p < 0.001). The current study revealed that oxidative stress and Lipid per-oxidation have an imperative role in the diabetic-related complication, specifically in the lens, may have a responsibility in the pathomechanism of early onset of cataracts coupled with diabetes mellitus.

Paraquat modulates immunological function in bone marrow-derived macrophages.

The herbicide paraquat (PQ) is known to affect the immune system. Many reports have indicated that PQ impacts on the viability and functions of the immune cells, however, the underlying mechanism in detail is still unknown. The aim of this study was to evaluate the effects of PQ on the free radical production, oxidative stress, cell death and pro-inflammatory gene expression of murine bone marrow-derived macrophages (BMDMs) from C57BL/6NJcl mice in vitro. BMDMs were incubated with PQ at 0, 200 and 400 µM concentrations for 24 h. Intracellular reactive oxygen species (ROS) production, apoptosis, cell viability, nitric oxide, inducible nitric oxide synthase (iNOS), and IL-6 expression levels were measured. The results revealed that PQ treatments led to a decrease in the cell viability and induced apoptotic cell death in a dose-dependent manner. Additionally, PQ also induced the generation of ROS. The mRNA level of the pro-inflammatory mediator genes iNOS and IL-6 were also elevated, while the level of lipid peroxide (malondialdehyde) production remained unaltered. Interestingly, the PQ treatment led to a decrease in the nitric oxide production. These results indicate that the increased cellular ROS production, due to the PQ treatment, induces apoptosis and the herbicide triggers production of iNOS and IL-6 in BMDMs.

Oxidative stress and biochemical indicators in blood of patients addicted to alcohol treated for acute ethylene glycol poisoning

Ethylene glycol (EG), in addition to its neurotoxic and nephrotoxic effects, evokes oxidative stress. The aim of this study was to assess the influence of the ethylene glycol on the biochemical indicators and oxidoreductive balance of patients treated for acute poisoning. The total study group consisted of 56 persons including 26 alcoholics who took EG as a substitute for ethyl alcohol in the course of alcohol dependence syndrome and 30 controls. Severity of poisoning, results of acid-base parameters, biochemical, and toxicological tests as well as biomarkers of the oxidative stress in blood were analyzed during the patients' hospitalization. The key issue was to assess the oxidative stress and biochemical disturbances caused by EG and the type of treatment applied in the course of poisoning. Significant changes in some parameters were found both at time of diagnosis and after treatment initiation (ethanol as an antidote and hemodialysis). The most important differences included the activity of hepatic parameters (aspartate aminotransferase, AST) and oxidative stress markers like catalase (CAT); correlation of the lipid peroxidation products level (TBARS) with urea concentration has been shown. On the last day of the hospitalization, in some cases, the mutual correlation between the evaluated markers were observed, for example, between alanine transaminase (ALT) and glutathione reductase (GR), and urea concentration and glutathione level (GSH/GSSG). The concentration of ions (H+) had a major impact on the oxidoreductive balance, correlating with the elevated GR and GSH/GSSG levels.