Levels Of Contractile Force Research Articles

Activity of right premotor-parietal regions dependent upon imagined force level: an fMRI study.

In this study, we utilized functional magnetic resonance imaging (fMRI) to measure blood oxygenation level-dependent (BOLD) signals. This allowed us to evaluate the relationship between brain activity and imagined force level. Subjects performed motor imagery of repetitive right hand grasping with three different levels of contractile force; 10%, 30%, and 60% of their maximum voluntary contraction (MVC). We observed a common activation among each condition in the following brain regions; the dorsolateral prefrontal cortex (DLPFC), ventrolateral prefrontal cortex (VLPFC), supplementary motor area (SMA), premotor area (PM), insula, and inferior parietal lobule (IPL). In addition, the BOLD signal changes were significantly larger at 60% MVC than at 10% MVC in the right PM, the right IPL, and the primary somatosensory cortex (SI). These findings indicate that during motor imagery right fronto-parietal activity increases as the imagined contractile force level is intensified. The present finding that the right brain activity during motor imagery is clearly altered depending on the imagined force level suggests that it may be possible to decode intended force level during the motor imagery of patients or healthy subjects.

Mechanosensing by Tropomyosin-Controlled Myosin Contractions

Control of cell growth, death or differentiation involves the integration of microenvironmental signals through cell motile processes to produce the desired cellular responses. In the case of cell-matrix interactions, the cell tests the matrix by asking several if-then questions such as is the matrix clustered, can force be generated and later what is the rigidity of the matrix. Using lipid-linked matrix ligands, we found that cells would not spread on the surface unless the liganded integrins were clustered and cells generated forces on the ligands by pulling to barriers (Yu et al., 2011. PNAS 108:20565). Upon activation of cell spreading, the flattening of the cells removed the folds in the membranes until a rise in membrane tension activated contraction to sense rigidity (Gauthier et al., 2011. PNAS 108:14467). Rigidity sensing involved local contraction units that pulled about 100 nm independent of rigidity (Ghassemi et al., 2012 PNAS 109:5325. Recently, we found that local contraction units resembled dynamic sarcomeres with alpha actinin at ends and myosin in the middle (Meacci et al., submitted). Further, generation of force involved repeated step-wise movements of myosin controlled by tropomyosin-1 (Wolfenson et al., submitted). Angstrom level measurements of fibroblasts pulling on elastomeric PDMS pillars showed that pillar displacement occurred in discrete steps of ∼1 nm. In contractile pairs, simultaneous steps of opposing pillars had a total step size of ∼2.2 nm, independent of rigidity. Changes in the stepping patterns on different rigidities indicated that the level of contractile force was critical for sensing pillar stiffness. Importantly, knockdown of tropomyosin-1 caused larger steps and increased forces that resulted in aberrant rigidity sensing. This indicates that the process of cell-matrix adhesion formation involves multiple, sequential steps with if-then decisions based upon the physical as well as biochemical properties of the matrix.

Changes in the centrifugal gating effect on somatosensory evoked potentials depending on the level of contractile force

In this study, we investigated the somatosensory evoked potentials (SEPs) during the preparatory period of self-initiated plantar flexion at different force levels of muscle contraction and elucidated the mechanism behind the centrifugal gating effect on somatosensory information processing. We recorded SEPs following stimulation of the tibial nerve at the popliteal fossa during the preparatory period of a 20% maximal voluntary contraction (MVC) and 50% MVC. The preparatory period was divided into two sub-periods based on the components of movement-related cortical potentials, the negative slope (NS sub-period) and the Bereitschaftspotential (BP sub-period). The subjects were instructed to concentrate on the movement and not to pay attention to the continuous electrical stimulation. Pre-movement SEPs were averaged separately during the two sub-periods under each MVC condition. The mean amplitudes of BP and NS were larger during the 50% MVC than the 20% MVC. As for the components of SEPs, during the NS sub-period the amplitude of P30 under the 50% MVC and N40 under both conditions were significantly smaller than that in the stationary sequence, and N40 amplitude was significantly smaller during the 50% MVC than the 20% MVC. During the BP sub-period, the amplitude of P30 and N40 during the 50% MVC was significantly smaller than during the stationary sequence, while it was not significantly different between the 20% and 50% MVCs. In conclusion, the extent of the centrifugal gating effect on SEPs was dependent on the activities of motor-related areas, which generated the NS and BP.

Motor unit firing rates and firing rate variability in the detection of neuromuscular disorders

We have used automatic decomposition electromyography (ADEMG) to study 41 muscles in 29 patients with well-defined peripheral and central motor disorders. In motor neuron diseases motor unit action potentials (MUAPs) showed increased amplitudes, firing rates and firing variability. Relatively large MUAPs sometimes were not identified by the computer program if they lacked sufficient high-frequency signal content, or were too variable in shape. In myopathies the MUAPs showed reduced amplitudes, durations and turns, and sometimes dramatic increases in firing rates. Also, the mean number of MUAPs per recording site was often increased, indicating excessive recruitment. In polymyositis (the best studied myopathy) the nature and magnitude of the MUAP shape and firing abnormalities were usually similar at different levels of contractile force, suggesting that motor units are affected without regard to recruitment order. In upper motor neuron paresis (multiple sclerosis), the shape properties of the MUAPs were normal, but mean firing rates were reduced, and firing variability increased. These findings confirm many of the traditional criteria for distinguishing neurogenic from myopathic disease electrophysiologically at the level of the individual MUAP. In addition, they demonstrate the potential diagnostic sensitivity of MUAP firing rate measurements for detecting neuromuscular dysfunction, and for differentiating between some cases of central and peripheral paresis, but not for distinguishing peripheral neurogenic from myopathic weakness, since firing rates tend to increase in both. Increased firing rate variability may be a marker of central or peripheral neurogenic weakness.

Decreased endothelium-dependent relaxation in New Zealand genetic hypertensive rats.

The relaxation response to endothelium-dependent (acetylcholine and the calcium ionophore A23187) and independent (sodium nitroprusside) vasodilators was examined in isolated aortic ring segments from age-matched genetically hypertensive (GH) and normotensive (N) rats (New Zealand strain). Tissues were initially contracted with methoxamine to achieve similar levels of contractile force. The IC20, IC40 and IC50 values for acetylcholine, A23187 and sodium nitroprusside were shifted significantly to the right (P less than 0.05) in aortic rings from GH rats compared to the corresponding values in N rats. The maximal relaxation achieved by acetylcholine and A23187 was significantly depressed in aortas from GH rats (P less than 0.05). Sodium nitroprusside elicited the maximal relaxation in both groups of tissues. These results demonstrate that there exists a generalized defect in the relaxant ability of vascular smooth muscle from GH rats. In addition, our findings suggest that this defect is coupled with a decreased responsiveness to endothelium-dependent vasodilators in this particular animal model of hypertension.